Furanone derivative

ABSTRACT

To provide a novel furanone derivative, and a medicine including the same. The furanone derivative is represented by the formula (I): 
                         
wherein A represents —COOR1 or a hydrogen atom; R1 represents a hydrogen atom, an optionally substituted hydrocarbon group, or an optionally substituted heterocycle; R2 and R3 are the same or different and each independently represent a hydrogen atom, an optionally substituted hydrocarbon group, an optionally substituted phenyl group, an optionally substituted heterocycle, an optionally substituted heterocyclic fused ring, or an optionally substituted amino group; or alternatively, R2 and R3, taken together with the nitrogen atom to which they are attached, may form an optionally substituted heterocycle or an optionally substituted heterocyclic fused ring; and R4 represents a hydrogen atom or a halogen atom; with the proviso that when A represents —COOR1, R2 and R3 are not optionally substituted amino groups at the same time, and when A represents a hydrogen atom, R3 represents a hydrogen atom.

The present application is a Reissue of application Ser. No. 14/008,488, filed Mar. 30, 2012, issued as U.S. Pat. No. 8,742,113 on Jun. 3, 2014.

TECHNICAL FIELD

The present invention relates to a medicine, particularly a novel furanone derivative having an inhibitory effect on Cdc7 or a pharmaceutically acceptable salt thereof.

BACKGROUND ART

Cancer is a group of diseases caused by uncontrolled, unlimited growth of cells within a living body. Since cancer cells usually grow faster than normal cells, cancers would be capable of being treated by controlling the replication of DNA during the cell division, particularly during the division of chromosomes. Actually, gemcitabine, which has the effect of inhibiting DNA replication, is widely used in the treatment of non-small cell lung cancer, pancreatic cancer, biliary tract cancer, bladder cancer, breast cancer, ovarian cancer, or others.

Cdc7 is a serine-threonine protein kinase and is an enzyme which is essential for the initiation of DNA replication in the cell cycle. Specifically, Cdc7 forms a complex with cofactors such as Dbf4 (ASK), and phosphorylates its substrate, MCM (mini-chromosome maintenance) proteins. It is supposed that this phosphorylation results in assembly of Cdc45 and a DNA polymerase on the DNA to form an MCM complex, thereby initiating the DNA replication (see Non Patent Literature 1). Furthermore, it has been shown in a recent study that Cdc7 plays an important role not only in the replication of DNA, but also in DNA damaging pathways (see Non Patent Literature 2).

Recently, Cdc7 has drawn attention as a target of anticancer agents, and active researches on Cdc7 have been made. For example, it was found that CDC7 is overexpressed not only in common established cell lines derived from human tumors, but also in cells taken from live tissues, such as breast cancer, colon cancer, and lung cancer (see Non Patent Literature 3). Particularly, it was shown, in more recent days, that CDC7 is overexpressed in p53-mutated triple negative (ER−/PR−/Her2−) breast cancer cells (see Non Patent Literature 4), and thus it has been expected that Cdc7 will be a promising target molecule against a triple negative type of breast cancer, which has been considered to be difficult to treat. Actually, it was observed that in experiments for suppressing the expression of Cdc7 using RNA interference techniques, the arresting of the cell cycle was induced when the expression of Cdc7 was inhibited. More importantly, the Cdc7 inhibition using RNA interference techniques suppressed the growth of human tumor cells, such as HeLa and HCT116 cells, and exhibited only limited effects on normal cells (normal human skin fibroblasts) (see Non Patent Literature 5).

Therefore, selective inhibitors of Cdc7 can be expected to have an effective therapeutic effect against various types of cancer. Although various compounds having an inhibitory effect on Cdc7 have been reported in the past, there are no reports in which novel furanone derivatives of the present invention or pharmaceutically acceptable salts thereof have an inhibitory effect on Cdc7.

CITATION LIST Non Patent Literature

[Non Patent Literature 1]

-   H. Masai et al., Journal of Cellular Ohysiology, 190, 2002, 287-296

[Non Patent Literature 2]

-   J M. Kim et al., Oncogene, 27, 2008, 3475-3482

[Non Patent Literature 3]

-   D Bonte et al., Neoplasia, 10, 2008, 920-931

[Non Patent Literature 4]

-   S. Rodriguez-Acebes et al., The American Journal of Pathology, 177,     2010, 2034-2045

[Non Patent Literature 5]

-   A. Montagnoli et al., Cancer Research, 64, 2004, 7110-7116

SUMMARY OF INVENTION Technical Problem

An object of the present invention is to provide a medicine, particularly a novel furanone derivative having an inhibitory effect on Cdc7 or a pharmaceutically acceptable salt thereof.

Solution to Problem

The present invention is achieved by the following (1) to (3):

(1) A furanone derivative or a pharmaceutically acceptable salt thereof, represented by the formula (I):

wherein A represents —COOR1 or a hydrogen atom; R1 represents a hydrogen atom, an optionally substituted hydrocarbon group, or an optionally substituted heterocycle; R2 and R3 are the same or different and each independently represent a hydrogen atom, an optionally substituted hydrocarbon group, an optionally substituted phenyl group, an optionally substituted heterocycle, an optionally substituted heterocyclic fused ring, or an optionally substituted amino group; or alternatively, R2 and R3, taken together with the nitrogen atom to which they are attached, may form an optionally substituted heterocycle or an optionally substituted heterocyclic fused ring; and R4 represents a hydrogen atom or a halogen atom; with the proviso that when A represents —COOR1, R2 and R3 are not optionally substituted amino groups at the same time, and when A represents a hydrogen atom, R3 represents a hydrogen atom; (2) The furanone derivative or a pharmaceutically acceptable salt thereof according to (1), wherein A is —COOR1; and (3) The furanone derivative or a pharmaceutically acceptable salt thereof according to (1), wherein A is a hydrogen atom.

Advantageous Effects of Invention

The present inventors have made various studies in order to solve the above-mentioned problem, and found that novel furanone derivatives represented by the formula (I) and pharmaceutically acceptable salts thereof had excellent inhibitory effect on Cdc7, resulting in completion of the present invention. The compounds provided by the present invention are capable of controlling the growth of cells. Therefore, the compounds of the present invention having an inhibitory effect on Cdc7 will be useful as a medicine, particularly an agent for the treatment of diseases derived from abnormal growth of cells, such as cancers.

DESCRIPTION OF EMBODIMENTS

The present invention will be described in detail below.

A novel furanone derivative of the present invention is a compound represented by the formula (I):

wherein A represents —COOR1 or a hydrogen atom; R1 represents a hydrogen atom, an optionally substituted hydrocarbon group, or an optionally substituted heterocycle; R2 and R3 are the same or different and each independently represent a hydrogen atom, an optionally substituted hydrocarbon group, an optionally substituted phenyl group, an optionally substituted heterocycle, an optionally substituted heterocyclic fused ring, or an optionally substituted amino group; or alternatively, R2 and R3, taken together with the nitrogen atom to which they are attached, may form an optionally substituted heterocycle or an optionally substituted heterocyclic fused ring; and R4 represents a hydrogen atom or a halogen atom; with the proviso that when A represents —COOR1, R2 and R3 are not optionally substituted amino groups at the same time, and when A represents a hydrogen atom, R3 represents a hydrogen atom. In the formula (I), an optionally substituted hydrocarbon group includes, for example, a) a linear or branched alkyl group having 1 to 6 carbons (for example, methyl, ethyl, isopropyl, tert-butyl, hexyl, etc.); b) a linear or branched alkenyl group having 1 to 6 carbons (for example, vinyl, allyl, isopropenyl, 2-butenyl, etc.); c) an alkynyl group having 2 to 6 carbons (for example, ethynyl, propargyl, 2-butynyl, etc.); d) a cycloalkyl group having 3 to 8 carbons (for example, cyclopropyl, cyclopentyl, cyclohexyl, cycloheptyl, etc.); e) a cycloalkenyl group having 3 to 8 carbons (for example, cyclohexenyl, cycloheptenyl, etc.); f) an aralkyl group, the aryl moiety of which is an aryl having 6 to 14 carbons (for example, phenyl, naphthyl, indenyl, etc.) and the alkylene moiety of which has the same meaning as a group where one hydrogen atom has removed from the above-mentioned alkyl group; and others.

The heterocyclic moiety of an optionally substituted heterocycle includes an alicyclic heterocyclic group and an aromatic heterocyclic group. An alicyclic heterocyclic group is, for example, a 3- to 8-membered heterocyclic group containing at least one heteroatom selected from a nitrogen atom, a sulfur atom, and an oxygen atom. Specific examples of the alicyclic heterocyclic group include pyrrolidinyl, piperidyl, piperazinyl, morpholinyl, thiomorpholinyl, etc. An aromatic heterocyclic group is, for example, a 5- or 6-membered monocyclic aromatic heterocyclic group containing at least one heteroatom selected from a nitrogen atom, a sulfur atom, and an oxygen atom. Specific examples of the aromatic heterocyclic group include imidazolyl, pyrazolyl, thienyl, thiazolyl, pyridyl, etc.

The heterocyclic fused ring moiety of an optionally substituted heterocyclic fused ring is, for example, a fused heterocyclic group which is bicyclic by fusing 3- to 8-membered rings and which contains at least one heteroatom selected from a nitrogen atom, a sulfur atom, and an oxygen atom. Specific examples of the fused heterocyclic group include benzothiophenyl, benzimidazolyl, indazolyl, benzoxazolyl, benzothiazolyl, indolyl, isoquinolyl, phthalimide, etc.

An optionally substituted amino group is, for example, an amino group having a linear, branched, or cyclic alkyl, aryl, or heteroaryl group which is substituted or unsubstituted and containing 1 to 6 carbons, for example, an amino group to which an alkyl group, an alkylamino group, an aryl group, a heteroaryl group, a heterocyclic group, a heterocyclic fused ring group, or the like which is unsubstituted or substituted with one or more substituents may be attached. The “one or more substituents” in these groups attached to an amino group may be any one or more substituents which are the same or different, unless otherwise specified, and include, for example, a halogen atom, a substituted or unsubstituted alkyl group, a substituted or unsubstituted alkenyl group, a substituted or unsubstituted alkynyl group, a substituted or unsubstituted alkoxy group, an amino group, a nitro group, a cyano group, a hydroxy group, a substituted or unsubstituted alkylamino group, a carbamoyl group, a carboxyl group, a formyl group, an acetyl group, a benzoyl group, etc.

The “substituent(s)” in an optionally substituted hydrocarbon group, an optionally substituted heterocycle, an optionally substituted phenyl group, or an optionally substituted heterocyclic fused ring is/are be one or more substituents of any type which are allowed to be located at any chemically acceptable positions, unless otherwise specified. When two or more substituents are present, these substituents may be the same or different and include, for example, a halogen atom, a substituted or unsubstituted alkyl group, a substituted or unsubstituted alkoxy group, a substituted or unsubstituted amino group, a nitro group, a cyano group, a hydroxy group, a substituted or unsubstituted alkylamino group, a carbamoyl group, a carboxyl group, a formyl group, an acetyl group, a benzoyl group, etc.

The heterocyclic group in the case where R2 and R3, taken together with the nitrogen atom to which they are attached, form an optionally substituted heterocycle or an optionally substituted heterocyclic fused ring is, for example, a 3- to 8-membered heterocyclic group containing at least one heteroatom selected from a nitrogen atom, a sulfur atom, and an oxygen atom, or a fused alicyclic heterocyclic group which is bicyclic by fusing 3- to 8-membered rings and which contains at least one heteroatom selected from a nitrogen atom, a sulfur atom, and an oxygen atom. Specific examples of such heterocyclic groups include pyrrolidinyl, piperidyl, morpholinyl, thiomorpholinyl, azepinyl, diazepinyl, dihydroisoquinolyl, tetrahydroisoquinolyl, tetrahydroquinolyl, isoindolinyl, indolinyl, tetrahydrobenzazepinyl, benzazepinyl, benzodiazepinyl, benzoxyazepinyl, benzothiazepinyl, etc.

A halogen atom includes, for example, fluorine, chlorine, bromine, and the like.

A compound (I) of the present invention may have isomers, for example, depending on the type of its substituents. In the specification, such a compound is sometimes described by the chemical structure of only one of its isomeric forms. However, the present invention includes all of the structurally possible isomers of such a compound (geometrical isomers, optical isomers, tautomers, etc.), and also include its individual isomers or mixtures thereof.

Also, the present invention encompasses stereoisomers of a compound of the present invention represented specifically by the formulae (I-Z) and (I-E), and mixtures thereof.

A pharmaceutically acceptable salt of a compound (I) of the present invention includes a salt with an inorganic acid, such as hydrochloric acid, sulfuric acid, carbonic acid, and phosphoric acid, and a salt with an organic acid, such as formic acid, acetic acid, fumaric acid, maleic acid, methanesulfonic acid, and p-toluenesulfonic acid. Also included in the present invention are, for example, a salt with an alkali metal, such as sodium and potassium, a salt with an alkaline earth metal, such as magnesium and calcium, a salt with an organic amine, such as a lower alkyl amine and a lower alcohol amine, and a salt with a basic amino acid, such as lysine, arginine, and ornithine, and in addition, an ammonium salt.

Compounds (I) of the present invention and pharmaceutically acceptable salts thereof can be produced, for example, by the methods mentioned below. In the production methods mentioned below, when a defined group is changed under conditions where the method is performed, or when a defined group is not suitable for performing the method, the production can be easily achieved by applying methods usually used in organic synthetic chemistry, such as procedures for protection and deprotection of functional groups [T. W. Greene, Protective Groups in Organic Synthesis, 3rd Edition, John Wiley & Sons, Inc., 1999]. In addition, the order of reaction steps, for example, those for introducing a substituent or substituents, may be changed as needed.

A compound (Ia) wherein A is —COOR1 can be produced, for example, by scheme 1:

wherein R1, R2, R3 and R4 have the same meaning as mentioned above.

A compound (Ia) of the present invention can be obtained by heating and reacting a compound (III) and 1 to 5, preferably 1 to 1.5, molar equivalents of a compound (IV) in a solvent under conditions for a Knoevenagel condensation reaction, that is, in the presence of a catalytic base such as piperidine. The solvent can be any solvent which is inert in the reaction, and is not limited in particular. For example, a lower alcohol, preferably ethanol, can be used as the solvent. As the base, piperidine or proline, for example, can be used in an amount of from a catalytic amount to an equivalent amount relative to the compound (III). The reaction can be carried out in the range of from room temperature to reflux temperature and for a period of 3 hours to 2 weeks. Preferably, the reaction can be carried out for 1 to 3 days under conditions of reflux in ethanol, thereby to synthesize the compound. In addition, this reaction can also be performed under other usual conditions used in the Knoevenagel condensation reaction, for example, under acidic conditions using hydrochloric acid, acetic acid, or the like, to produce the compound.

The compound (IV) which can be used as one starting material in scheme 1 is commercially available (for example, from SIGMA-ALDRICH) or can be obtained by known methods (see, for example, Rajesh H. Bahekar et al., Bioorganic & Medicinal Chemistry, 15 (21), 6782-6795 (2007); and Seung-Jun Oh et al., Bioorganic & Medicinal Chemistry, 12 (21), 5505-5513 (2004)).

On the other hand, the compound (III) which can be used as the other starting material in scheme 1 can be produced, for example, by the procedures shown in scheme 2 or 3.

wherein R1, R2, and R3 have the same meaning as mentioned above, and R1′ represents a substituted or unsubstituted lower alkyl group. Step 2-1

A compound (VII) can be obtained by converting a malonic diester (V) to its enolate with a base, such as sodium hydride, in a solvent, such as anhydrous tetrahydrofuran, followed by reaction with chloroacetyl chloride (VI).

Step 2-2

The compound (III) can be obtained by reacting the compound (VII) obtained in the previous step and an amine (VIII) in an amount of from an equivalent amount to an excess amount, preferably in an amount of 1.2 to 3 molar equivalents, in a solvent at room temperature or at heated temperature.

The solvent can be any solvent which is inert in the reaction, and is not limited in particular. For example, tetrahydrofuran, dimethylformamide, ethanol, and the like can be used as the solvent.

The reaction is dependent on the reactivity of the amine (VIII) used, and generally is completed in a period of 1 hour to 1 day at a temperature of from room temperature to the reflux temperature of the solvent. When the reaction progresses at a significantly slow rate, the desired product can be obtained by adding a base, such as sodium hydride, sodium hydroxide, triethylamine, or the like, in an amount of from an equivalent amount to an excess amount.

Further, the compound (III) can also be produced by mean of a sequence of reactions without isolating the compound (VII), by known methods (see, for example, Sheng-Chu Kuo et al., Chem. Pharm. Bull., 36 (11), 4403-4407 (1988)) or their modified methods. That is, the compound (III) can be obtained by adding the amine (VIII) in an amount of from an equivalent amount to an excess amount, preferably in an amount of 1.2 to 3 molar equivalents, to the solution after the reaction in step 2-1, and performing the reaction at room temperature or at heated temperature.

The malonic diester (V) and the amine (VIII) which can be used as the starting materials in scheme 2 are commercially available or can be obtained by known methods.

wherein R1 and R2 have the same meaning as mentioned above.

Among the compounds (III), a compound (IIIa) wherein R3 is a hydrogen atom can be produced by known methods (see, for example, Robert A. Mack et al., J. Med. Chem., 31 (10), 1910-1918 (1988)) or their modified methods. That is, the compound (IIIa) can be obtained by cyclocondensation of a compound (IX) with 1 to 5, preferably 1 to 1.5, molar equivalents of isocyanate (X) in a solvent in the presence of a base, such as triethylamine.

The solvent can be any solvent which is inert in the reaction, and is not limited in particular. For example, diethyl ether or ethyl acetate, or mixed solvents thereof can be used as the solvent.

The reaction can be carried out in the range of from ice-cooled temperature to reflux temperature and for a period of 30 minutes to 1 day. Preferably, the reaction can be carried out at room temperature for 1 to 3 hours, thereby to synthesize the compound.

The compound (IX) and isocyanate (X) that are the starting materials in scheme 3 are commercially available or can be obtained by known methods.

Further, a compound (Ia) of the present invention can also be produced by a transesterification reaction of a compound (Ia′), which is a lower alkyl ester compound, as shown in scheme 4:

wherein R1, R2, R3, and R4 have the same meaning as mentioned above, and R1′ represents a substituted or unsubstituted lower alkyl group.

A compound (Ia) of the present invention can be produced by a transesterification reaction of the compound (Ia′) by known methods (see, for example, Takanori Iwasaki et al., J. Org. Chem., 73 (13), 5147-5150 (2008)) or their modified methods. That is, the compound (Ia) can be obtained by heating and reacting the compound (Ia′) and an excess amount, preferably more than 10 molar equivalents, of an alcohol (XI) in a solvent in the presence of a tetranuclear zinc cluster catalyst. The solvent can be any solvent which is inert in the reaction, and is not limited in particular. For example, dimethylacetamide, 1,4-dioxane, diisopropyl ether, or the like can be used as the solvent. Alternatively, the alcohol (XI) may be used as the solvent. As the tetranuclear zinc cluster catalyst, catalysts which are commercially available (for example, under a product name of ZnTAC24, from STREM CHEMICALS) or are produced by the method described in the above-mentioned reference are used and added in a catalytic amount, preferably in an amount of 1 to 10% molar equivalents. Further, it is also possible that a tertiary amine, such as dimethylaminopyridine or triethylamine, is added in an amount of from a catalytic amount to 1 molar equivalent to accelerate the reaction.

The reaction can be carried out in the range of from room temperature to reflux temperature and for a period of 1 hour to 1 week. Preferably, the reaction can be carried out for 1 to 3 days under reflux conditions, thereby to synthesize the compound. Alternatively, the reaction may also be carried out using a microwave reactor, for example, for a period of from several minutes to several hours under temperature conditions of 60 to 150° C., thereby to synthesize the compound.

This reaction can also be carried out under other usual conditions used in the transesterification reaction, as shown in scheme 5, for example, under acidic or basic conditions, or under conditions using a catalyst, such as tetravalent titanium.

wherein R1′, R2, R3, and R4 have the same meaning as mentioned above.

A compound (Ib) of the present invention can be obtained by hydrolysis of a compound (Ia′), which is a lower alkyl ester compound, under reaction conditions used in usual organic synthetic chemistry (using methods described in, for example, T. W. Greene, Protective Groups in Organic Synthesis, 3rd Edition, John Wiley & Sons, Inc., 1999, p. 377, or their modified methods). That is, the compound (Ib) can be obtained by reacting the compound (Ia′) and a base or acid in an amount of from an equivalent amount to an excess amount in a solvent at a temperature between 0° C. and the boiling point of the solvent used.

The solvent can be any solvent which is inert in the reaction, and is not limited in particular. For example, 1,4-dioxane, tetrahydrofuran, various alcohols, or the like can be used as the solvent.

As the base or acid, use can be made of, for example, sodium hydroxide, potassium hydroxide, or hydrochloric acid.

The reaction can be carried out at a temperature between 0° C. and the boiling point of the solvent used and for a period of 1 hour to 1 week. Preferably, the reaction can be carried out for 1 hour to 1 day under reflux conditions, thereby to synthesize the compound.

Among the compounds (I) of the present invention, a compound (Ic) wherein A and R3 are hydrogen atoms can be produced, for example, as shown in scheme 6:

wherein R2 and R4 have the same meaning as mentioned above, and R1″ represents a hydrogen atom or a substituted or unsubstituted lower alkyl group.

A compound (Ic) of the present invention can be synthesized from a compound (Ia″), which is a compound (Ia) wherein R1 is a substituted or unsubstituted lower alkyl ester group and R3 is hydrogen, using known methods (see, for example, Sheng-Chu Kuo et al., Chem. Pharm. Bull., 38 (2), 340-341 (1990)) or their modified methods.

The reaction can be carried out by heating a solution or suspension of a compound (Ia″) in N,N-dimethylformamide or N,N-dimethylacetamide at a temperature between 100° C. and the boiling point of the solvent used and for a period of 1 to 24 hours. Preferably, the reaction can be carried out for 1 to 12 hours under reflux conditions, thereby to synthesize the compound.

This reaction can also be carried out by heating and stirring in the presence of a base. For example, the reaction can be carried out by heating to reflux in an alcohol solvent, such as ethanol, in the presence of a highly-concentrated aqueous solution of potassium hydroxide, thereby to synthesize compound.

Moreover, a compound (I) of the present invention which has a desired functional group at a desired position can be obtained by combining the above-mentioned methods as appropriate and carrying out procedures usually used in organic synthetic chemistry (for example, reactions for alkylation of amino groups, reactions for oxidation of an alkylthio group to the corresponding sulfoxide or sulfone group, reactions for converting an alkoxy group to a hydroxy group or vice versa).

Compounds (I) of the present invention or pharmaceutically acceptable salts thereof can be used as medicines, particularly anti-tumor agents, in the form of conventional pharmaceutical preparations for oral administration or for parenteral administration, such as instillation.

Pharmaceutical preparations for oral administration include solid formulations, such as tablets, granules, powders, and capsules, and liquid formulations, such as syrups. These formulations can be prepared by conventional methods. Solid formulations can be prepared using conventional pharmaceutical carriers like lactose, starches such as corn starch, crystalline cellulose such as microcrystalline cellulose, hydroxypropylcellulose, calcium carboxymethylcellulose, talc, magnesium stearate, and others. Capsules can be prepared by encapsulating the granules or powders thus prepared. Syrups can be prepared by dissolving or suspending a compound (I) of the present invention or a pharmaceutically acceptable salt thereof in an aqueous solution containing sucrose, carboxymethylcellulose, or the like.

Pharmaceutical preparations for parenteral administration include formulations for injection, such as instillation. Formulations for injection can also be prepared by conventional methods, and may be incorporated in tonicity adjusting agents (for example, mannitol, sodium chloride, glucose, sorbitol, glycerol, xylitol, fructose, maltose, mannose), stabilizing agents (for example, sodium sulfite, albumin), antiseptics (for example, benzyl alcohol, methyl p-hydroxybenzoate) as appropriate.

Compounds (I) of the present invention or pharmaceutically acceptable salts thereof are effective for medicines, particularly for the treatment of tumors. Tumors include solid tumors, such as breast cancer, colon cancer, and lung cancer, and hematological cancers, such as leukemia, lymphoma, and myeloma.

The amount of dosage of a compound (I) of the present invention or a pharmaceutically acceptable salt thereof can be varied, according to the severeness of the disease, the age and body weight of the patient, its dosage form, and others, and usually is in the range of 1 mg to 1,000 mg per day for adult humans, which can be administered once, or twice or thrice by oral route or by parenteral route.

EXAMPLES

The present invention will be more specifically described below by way of Examples and Test Examples, which are not intended to limit the present invention.

The identification of compounds was performed with hydrogen nuclear magnetic resonance (¹H-NMR) spectra and mass spectra (MS). ¹H-NMR spectra were measured at 400 MHz, unless otherwise specified. In the ¹H-NMR spectra, exchangeable hydrogens are sometimes not clearly observed, depending on the compound being measured and on the measurement conditions, and br denotes a broad signal. HPLC preparative chromatography was carried out using a commercial available ODS column, and fractions were collected using a water/methanol system (containing formic acid) as an eluent in a gradient mode, unless otherwise specified.

Example 1 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-4-oxo-2-(phenylamino)-4,5-dihydrofuran-3-carboxylate

To a solution of 7-azaindole-3-carboxaldehyde (0.10 g, 0.70 mmol) and ethyl 4-oxo-2-(phenylamino)-4,5-dihydrofuran-3-carboxylate (0.18 g, 0.70 mmol) in ethanol (3.0 mL), piperidine (0.083 mL, 0.84 mmol) was added at ambient temperature. The mixture was refluxed for 12 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with cold ethanol and isopropyl ether then dried to afford the titled compound (0.13 g, y. 48%).

¹H NMR (DMSO-d₆) δ (ppm) 12.30 (br. s, 1H), 10.58 (s, 1H), 8.23 (dd, J=1.25, 4.52 Hz, 1H), 7.98 (d, J=7.28 Hz, 1H), 7.79 (d, J=2.76 Hz, 1H), 7.52-7.64 (m, 4H), 7.43-7.51 (m, 1H), 6.80-6.99 (m, 2H), 4.28 (q, J=7.03 Hz, 2H), 1.30 (t, J=7.03 Hz, 3H); LCMS (m/z): 376.0 [M+H]⁺.

Example 2 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-methoxyphenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

First Step

Under a nitrogen atmosphere, to a mixed solution of ethyl 4-chloroacetoacetate (0.82 mL, 6.0 mmol) and 4-methoxyphenyl isocyanate 1.1 g, 7.2 mmol) in diethyl ether/ethyl acetate (10 mL/1.0 mL) that cooled with ice bath, triethylamine (0.96 mL, 6.9 mmol) was added dropwise. The reaction mixture was stirred at ambient temperature for 1.5 h then the precipitate was collected by filtration, washed with diethyl ether, 1M hydrochloric acid solution, water and diethyl ether, and then dried to afford ethyl 2-[(4-methoxyphenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate as solid (1.3 g, y. 78%).

¹H NMR (CDCl₃) δ (ppm) 10.11 (br. s, 1H), 7.24-7.33 (m, 2H), 6.90-6.96 (m, 2H), 4.65 (s, 2H), 4.38 (q, J=7.07 Hz, 2H), 3.83 (s, 3H), 1.40 (t, J=7.20 Hz, 3H); LCMS (m/z): 277.9 [M+H]⁺.

Second Step

To a solution of ethyl 2-[(4-methoxyphenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.28 g, 1.0 mmol) and 7-azaindole-3-carboxaldehyde (0.15 g, 1.0 mmol) in ethanol (4.0 mL), piperidine (0.12 mL, 1.2 mmol) was added at ambient temperature. The mixture was refluxed for 12 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol and diisopropyl ether then dried to afford the titled compound (0.092 g, y. 22%).

¹H NMR (DMSO-d₆) δ (ppm) 12.31 (br. s, 1H), 10.47 (s, 1H), 8.24 (dd, J=1.51, 4.52 Hz, 1H), 7.94 (d, J=7.53 Hz, 1H), 7.77 (d, J=2.76 Hz, 1H), 7.50 (d, J=9.03 Hz, 2H), 7.09 (d, J=8.78 Hz, 2H), 6.81-6.92 (m, 2H), 4.26 (q, J=7.03 Hz, 2H), 3.87 (s, 3H), 1.29 (t, J=7.03 Hz, 3H); LCMS (m/z): 406.0 [M+H]⁺.

Example 3 Ethyl 5-[(5-chloro-1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-4-oxo-2-(phenylamino)-4,5-dihydrofuran-3-carboxylate

First Step

To a mixed solution of 2-amino-5-chloropyridine (2.6 g, 0.020 mol) in acetic acid/water (7.8 mL/1.8 mL) that cooled with ice bath, conc.sulfuric acid (0.26 mL, 4.9 mmol) was added dropwise, and then periodic acid (0.95 g, 4.2 mmol) and iodine (2.0 g, 8.0 mmol) were added. The reaction mixture was stirred at 80° C. for 6 h. Cooled to ambient temperature, the reaction mixture was poured into ice water, and neutralized by aqueous 5M sodium hydroxide solution. After removal of the precipitate by filtration, the filtrate was extracted with ethyl acetate. The organic layer was washed with saturated sodium thiosulfate solution, aqueous 1M sodium hydroxide solution and brine then dried over sodium sulfate and concentrated to afford 2-amino-5-chloro-3-iodopyridine as solid (4.4 g, y. 85%).

¹H NMR (CDCl₃) δ (ppm) 7.96 (s, 1H), 7.82 (s, 1H), 4.93 (s, 2H); LCMS (m/z) 255.2 [M+H]⁺.

Second Step

Under a nitrogen atmosphere, to a solution of 2-amino-5-chloro-3-iodopyridine (4.2 g, 0.017 mol), copper(I) iodide (0.032 g, 0.17 mmol) and triethylamine (7.0 mL, 0.050 mol) in anhydrous tetrahydrofuran (10 mL), dichlorobis(triphenylphosphine)palladium(II) (0.12 g, 0.17 mmol) and ethynyl trimethylsilane (3.0 mL, 0.021 mol) were added and the mixture was stirred at ambient temperature for 2 h. The reaction mixture was diluted with diethyl ether and removed the precipitate by filtration with C elite. The filtrate was concentrated to afford 2-amino-5-chloro-3-[(trimethylsilyl)ethynyl]pyridine as solid (4.2 g).

¹H NMR (CDCl₃) δ (ppm) 7.94 (d, 1H, J=2.28 Hz), 7.49 (d, 1H, J=2.32 Hz), 5.02 (s, 2H), 0.18-0.25 (m, 9H); LCMS (m/z): 225.5 [M+H]⁺.

Third Step

Under a nitrogen atmosphere, to a solution of potassium tert-butoxide (4.0 g, 0.036 mol) in 1-methyl-2-pyrrolidone (8.0 mL) which stirred with heating at 80° C., a solution of 2-amino-5-chloro-3-[(trimethylsilyl)ethynyl]pyridine (4.0 g, 0.018 mol) in 1-methyl-2-pyrrolidone (22 mL) was added dropwise and the mixture was stirred for 1 h. Cooled to ambient temperature, the reaction mixture was diluted with brine and stirred, then extracted with diethyl ether for 3 times. The organic layer was washed with brine, dried over sodium sulfate and concentrated to afford 5-chloro-7-azaindole as solid (2.3 g, y. 85%).

¹H NMR (CDCl₃) δ (ppm) 9.46 (s, 1H), 8.26 (s, 1H), 7.91 (d, 1H, J=1.12 Hz), 7.35 (s, 1H), 6.46 (s, 1H); LCMS (m/z): 153.2 [M+H]⁺.

Fourth Step

To a solution of 5-chloro-7-azaindole (0.50 g, 3.3 mmol) in acetic acid (5.0 mL), hexamethylenetetramine (0.69 g, 4.9 mmol) was added at ambient temperature. The mixture was refluxed for 8 h. Cooled to ambient temperature, the reaction mixture was diluted with water, extracted with ethyl acetate for 2 times. The organic layer was washed with water and brine, dried over sodium sulfate and concentrated. The residue was purified by chromatography on silica gel(hexane/ethyl acetate) to afford 5-chloro-7-azaindole-3-carboxaldehyde as solid (0.13 g, y. 22%).

¹H NMR (CDCl₃) δ (ppm) 10.01 (s, 1H), 9.77 (br. s, 1H), 8.61 (d, 1H, J=2.04 Hz), 8.37 (d, 1H, J=2.08 Hz), 7.99 (d, 1H, J=2.64 Hz); LCMS (m/z): 181.2 [M+H]⁺.

Fifth Step

To a solution of 5-chloro-7-azaindole-3-carboxaldehyde (0.019 g, 0.11 mmol) and ethyl 4-oxo-2-(phenylamino)-4,5-dihydrofuran-3-carboxylate (0.025 g, 0.10 mmol) in ethanol (1.0 mL), piperidine (0.012 mL, 0.12 mmol) was added at ambient temperature. The mixture was refluxed for 2 days. Cooled to ambient temperature, solvents were removed under reduced pressure then the residue was purified by preparative HPLC to afford the titled compound (0.0020 g, y. 4.6%).

¹H NMR (DMSO-d₆) δ (ppm) 12.52 (br. s, 1H), 10.58 (s, 1H), 8.42 (d, J=2.26 Hz, 1H), 8.26 (d, J=2.26 Hz, 1H), 7.71 (d, J=2.51 Hz, 1H), 7.55-7.63 (m, 2H), 7.51 (t, J=7.78 Hz, 2H), 7.35-7.44 (m, 1H), 7.00 (s, 1H), 4.28 (q, J=7.03 Hz, 2H), 1.30 (t, J=7.15 Hz, 3H); LCMS (m/z): 410.0 [M+H]⁺.

Example 4 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-4-oxo-2-piperidino-4,5-dihydrofuran-3-carboxylate

First Step

Diethyl malonate (5.0 mL, 0.033 mol) was added dropwise to a solution of sodium hydride (60% w/w in oil, 2.7 g, 0.066 mol) in anhydrous tetrahydrofuran (50 mL) that cooled with ice bath. The mixture was refluxed for 5 min. The reaction mixture was cooled with ice bath, chloroacetyl chloride (2.8 mL, 0.035 mol) was added dropwise to the reaction mixture and the mixture was stirred for 1 h then stirred at 45° C. for 1 h. The reaction mixture was cooled with ice bath again then piperidine (3.9 mL, 0.040 mol) was added dropwise and stirred at ambient temperature for further 12 h. The reaction mixture was diluted with aqueous saturated sodium bicarbonate solution, and extracted with ethyl acetate for 2 times then extracted with chloroform. The organic layer was washed with brine, dried over sodium sulfate and concentrated. The residue was purified by chromatography on silica gel(chloroform/methanol) to afford ethyl 4-oxo-2-piperidino-4,5-dihydrofuran-3-carboxylate as solid (3.5 g, y. 45%).

¹H NMR (DMSO-d₆) δ (ppm) 4.53 (s, 2H), 4.10 (q, J=7.03 Hz, 2H), 3.48-3.60 (m, 4H), 1.56-1.67 (m, 6H), 1.20 (t, J=7.03 Hz, 16H); LCMS (m/z): 240.0 [M+H]⁺.

Second Step

To a solution of ethyl 4-oxo-2-piperidino-4,5-dihydrofuran-3-carboxylate (0.10 g, 0.42 mmol) and 7-azaindole-3-carboxaldehyde (0.061 g, 0.42 mmol) in ethanol (2.0 mL), piperidine (0.041 mL, 0.42 mmol) was added at ambient temperature. The mixture was refluxed for 24 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol and diisopropyl ether then dried to afford the titled compound (0.045 g, y. 29%).

¹H NMR (DMSO-d₆) δ (ppm) 12.29 (br. s, 1H), 8.37 (dd, J=1.25, 8.03 Hz, 1H), 8.31 (dd, J=1.51, 4.52 Hz, 1H), 7.96 (d, J=2.76 Hz, 1H), 7.19 (dd, J=4.64, 7.91 Hz, 1H), 6.84 (s, 1H), 4.16 (q, J=7.19 Hz, 2H), 3.70-3.80 (m, 4H), 1.65-1.78 (m, 6H), 1.24 (t, J=7.03 Hz, 3H); LCMS (m/z): 368.0 [M+H]⁺.

Example 5 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(3-methoxyphenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-[(3-methoxyphenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.28 g, 1.0 mmol) which similarly prepared according to the procedure described in the Example 2, First step and 7-azaindole-3-carboxaldehyde (0.15 g, 1.2 mmol) in ethanol (4.0 mL), piperidine (0.12 mL, 1.2 mmol) was added at ambient temperature. The mixture was refluxed for 2 days. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol and diisopropyl ether then dried to afford the titled compound (0.17 g, y. 29%).

¹H NMR (DMSO-d₆) δ (ppm) 12.34 (br. s, 1H), 10.52 (s, 1H), 8.25 (dd, J=1.38, 4.64 Hz, 1H), 8.03 (d, J=7.03 Hz, 1H), 7.84 (d, J=2.76 Hz, 1H), 7.45 (t, J=8.03 Hz, 1H), 7.13-7.26 (m, 2H), 7.04 (dd, J=2.01, 8.28 Hz, 1H), 6.86-6.95 (m, 2H), 4.27 (q, J=7.03 Hz, 2H), 3.77 (s, 3H), 1.30 (t, J=7.15 Hz, 3H); LCMS (m/z): 406.0 [M+H]⁺.

Example 6 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-4-oxo-2-(2-pyridinylamino)-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 4-oxo-2-(2-pyridinylamino)-4,5-dihydrofuran-3-carboxylate (0.080 g, 0.32 mmol) which similarly prepared according to the procedure described in the Example 4, First step and 7-azaindole-3-carboxaldehyde (0.047 g, 0.32 mmol) in ethanol (2.0 mL), piperidine (0.038 mL, 0.39 mmol) was added at ambient temperature. The mixture was refluxed for 10 h. Cooled to ambient temperature, the precipitate was removed by filtration and the filtrate was purified by preparative HPLC to afford the titled compound as solid (0.0030 g, y. 2.5%).

¹H NMR (DMSO-d₆) δ (ppm) 12.41 (br. s, 1H), 10.74 (br. s, 1H), 8.60 (d, J=3.26 Hz, 1H), 8.35 (d, J=7.78 Hz, 1H), 8.30 (dd, J=1.38, 4.64 Hz, 1H), 8.24 (br. s, 1H), 7.97 (dt, J=1.88, 7.84 Hz, 1H), 7.61 (d, J=8.03 Hz, 1H), 7.36-7.45 (m, 1H), 7.02-7.14 (m, 2H), 4.27 (q, J=7.03 Hz, 2H), 1.29 (t, J=7.03 Hz, 3H); LCMS (m/z): 377.0 [M+H]⁺.

Example 7 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(2-methoxyphenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-[(2-methoxyphenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.28 g, 1.0 mmol) which similarly prepared according to the procedure described in the Example 2, First step and 7-azaindole-3-carboxaldehyde (0.15 g, 1.2 mmol) in ethanol (4.0 mL), piperidine (0.12 mL, 1.2 mmol) was added at ambient temperature. The mixture was refluxed for 2 days. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol and diisopropyl ether then dried to afford the titled compound (0.30 g, y. 74%).

¹H NMR (DMSO-d₆) δ (ppm) 12.34 (br. s, 1H), 10.35 (s, 1H), 8.25 (dd, J=1.38, 4.64 Hz, 1H), 8.02 (d, J=7.03 Hz, 1H), 7.85 (d, J=2.76 Hz, 1H), 7.66 (dd, J=1.38, 7.91 Hz, 1H), 7.41-7.51 (m, 1H), 7.28 (d, J=7.53 Hz, 1H), 7.12 (t, J=7.65 Hz, 1H), 6.88-6.99 (m, 2H), 4.28 (q, J=7.03 Hz, 2H), 3.85 (s, 3H), 1.30 (t, J=7.15 Hz, 3H); LCMS (m/z): 406.0 [M+H]⁺.

Example 8 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-chlorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-[(4-chlorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.18 g, 0.61 mmol) which similarly prepared according to the procedure described in the Example 2, First step and 7-azaindole-3-carboxaldehyde (0.088 g, 0.61 mmol) in ethanol (10 mL), piperidine (0.0044 mL, 0.044 mmol) was added at ambient temperature. The mixture was refluxed for 6 days. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol and hexane then dried to afford the titled compound (0.061 g, y. 23%).

¹H NMR (DMSO-d₆) δ (ppm) 12.33 (br. s, 1H), 10.61 (s, 1H), 8.26 (d, J=3.26 Hz, 1H), 7.96 (d, J=7.53 Hz, 1H), 7.82 (d, J=2.76 Hz, 1H), 7.58-7.67 (m, 4H), 6.87-6.95 (m, 2H), 4.27 (q, J=7.03 Hz, 2H), 1.29 (t, J=7.03 Hz, 3H); LCMS (m/z): 409.9 [M+H]⁺.

Example 9 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(3-chlorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-[(3-chlorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.10 g, 0.36 mmol) which similarly prepared according to the procedure described in the Example 2, First step and 7-azaindole-3-carboxaldehyde (0.051 g, 0.35 mmol) in ethanol (3.0 mL), piperidine (0.0035 mL, 0.035 mmol) was added at ambient temperature. The mixture was refluxed for 6 days. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol then dried to afford the titled compound (0.061 g, y. 23%).

¹H NMR (DMSO-d₆) δ (ppm) 12.36 (br. s, 1H), 10.63 (s, 1H), 8.26 (dd, J=1.38, 4.64 Hz, 1H), 8.01 (d, J=7.28 Hz, 1H), 7.82 (d, J=2.76 Hz, 1H), 7.76 (s, 1H), 7.50-7.61 (m, 3H), 6.89-6.96 (m, 2H), 4.28 (q, J=7.03 Hz, 2H), 1.30 (t, J=7.03 Hz, 3H); LCMS (m/z): 409.9 [M+H]⁺.

Example 10 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(benzylamino)-4-oxo-4,5-dihydrofuran-3-carboxylate hydrochloride

A solution of ethyl 2-(benzylamino)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.026 g, 0.10 mmol) which similarly prepared according to the procedure described in the Example 2, First step and 7-azaindole-3-carboxaldehyde (0.015 g, 0.10 mmol) in 2M hydrochloric acid in ethanol (1.0 mL) was refluxed for 4 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol and diisopropyl ether then dried to afford the titled compound (0.024 g, y. 56%).

¹H NMR (DMSO-d₆) δ (ppm) 12.25-12.56 (m, 1H), 9.54 (br. s, 1H), 8.25-8.42 (m, 2H), 7.86 (s, 1H), 7.35-7.48 (m, 4H), 7.24-7.33 (m, 1H), 7.08-7.18 (m, 1H), 6.87 (s, 1H), 4.86 (d, J=6.53 Hz, 2H), 4.23 (q, J=7.19 Hz, 2H), 1.27 (t, J=7.03 Hz, 3H); LCMS (m/z): 390.0 [M+H]⁺.

Example 11 5-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-4-oxo-2-piperidino-4,5-dihydrofuran-3-carboxylic acid

To a solution of the compound of Example 4 in dioxane (0.5 mL), 2M sodium hydroxide solution (0.50 mL, 1.0 mmol) was added at ambient temperature. The mixture was stirred at 110° C. for 1.5 h. Cooled to ambient temperature, the reaction mixture was neutralized by 2M hydrochloric acid solution then the solvent was removed under reduced pressure. The residue was purified by preparative HPLC to afford the titled compound (0.0044 g, y. 45%).

¹H NMR (DMSO-d₆) δ (ppm) 12.24 (br. s, 1H), 8.39 (d, J=8.03 Hz, 1H), 8.30 (dd, J=1.38, 4.64 Hz, 1H), 7.92 (d, J=2.26 Hz, 1H), 7.19 (dd, J=4.77, 8.03 Hz, 1H), 6.73 (s, 1H), 3.35-3.62 (m, 4H), 1.45-1.69 (m, 6H); LCMS (m/z): 339.9 [M+H]⁺.

Example 12 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(ethylamino)-4-oxo-4,5-dihydrofuran-3-carboxylate hydrochloride

To a solution of ethyl 2-(ethylamino)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.033 g, 0.17 mmol) which similarly prepared according to the procedure described in the Example 2, First step and 7-azaindole-3-carboxaldehyde (0.024 g, 0.17 mmol) in ethanol (1.5 mL), 2M hydrochloric acid in ethanol (0.17 mL, 0.34 mmol) was added at ambient temperature. The mixture was refluxed for 4 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol and diisopropyl ether then dried to afford the titled compound (0.026 g, y. 42%).

¹H NMR (DMSO-d₆) δ (ppm) 12.46 (br. s, 1H), 9.03 (t, J=6.02 Hz, 1H), 8.45-8.54 (m, 1H), 8.35 (dd, J=1.25, 4.77 Hz, 1H), 8.01 (d, J=1.25 Hz, 1H), 7.25 (dd, J=4.77, 8.03 Hz, 1H), 6.90 (s, 1H), 4.25-4.50 (m, 1H), 4.21 (q, J=7.19 Hz, 2H), 3.61-3.71 (m, 2H), 1.30 (t, J=7.15 Hz, 3H), 1.26 (t, J=7.03 Hz, 3H); LCMS (m/z): 328.0 [M+H]⁺.

Example 13 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-4-oxo-2-(phenethylamino)-4,5-dihydrofuran-3-carboxylate hydrochloride

To a solution of ethyl 4-oxo-2-(phenethylamino)-4,5-dihydrofuran-3-carboxylate (0.041 g, 0.15 mmol) which similarly prepared according to the procedure described in the Example 4, First step and 7-azaindole-3-carboxaldehyde (0.022 g, 0.15 mmol) in ethanol (1.0 mL), 2M hydrochloric acid in ethanol (0.16 mL, 0.32 mmol) was added at ambient temperature. The mixture was refluxed for 3 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol and diisopropyl ether then dried to afford the titled compound (0.023 g, y. 32%).

¹H NMR (DMSO-d₆) δ (ppm) 12.45 (br. s, 1H), 9.02 (t, J=6.15 Hz, 1H), 8.40 (d, J=6.78 Hz, 1H), 8.33 (dd, J=1.38, 4.64 Hz, 1H), 7.94 (d, J=2.26 Hz, 1H), 7.23-7.33 (m, 4H), 7.11-7.22 (m, 2H), 6.84 (s, 1H), 4.21 (q, J=7.19 Hz, 2H), 3.85 (q, J=6.94 Hz, 2H), 3.00 (t, J=7.28 Hz, 2H), 1.25 (t, J=7.03 Hz, 3H); LCMS (m/z): 404.0 [M+H]⁺.

Example 14 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-4-oxo-2-pyrrolidinyl-4,5-dihydrofuran-3-carboxylate hydrochloride

To a solution of ethyl 4-oxo-2-pyrrolidinyl-4,5-dihydrofuran-3-carboxylate (0.034 g, 0.15 mmol) which similarly prepared according to the procedure described in the Example 4, First step and 7-azaindole-3-carboxaldehyde (0.022 g, 0.15 mmol) in ethanol (1.0 mL), 2M hydrochloric acid in ethanol (0.16 mL, 0.32 mmol) was added at ambient temperature. The mixture was refluxed for 3 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol and diisopropyl ether then dried to afford the titled compound (0.038 g, y. 61%).

¹H NMR (DMSO-d₆) δ (ppm) 12.38 (br. s, 1H), 8.46 (d, J=8.03 Hz, 1H), 8.33 (dd, J=1.38, 4.64 Hz, 1H), 7.95 (d, J=2.26 Hz, 1H), 7.23 (dd, J=4.77, 7.78 Hz, 1H), 6.84 (s, 1H), 4.16 (q, J=7.03 Hz, 2H), 3.79-3.98 (m, 3H), 3.63-3.73 (m, 2H), 1.92-2.05 (m, 4H), 1.24 (t, J=7.03 Hz, 3H); LCMS (m/z): 354.0 [M+H]⁺.

Example 15 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(2-chlorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2[(2-chlorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.14 g, 0.50 mmol) which similarly prepared according to the procedure described in the Example 2, First step and 7-azaindole-3-carboxaldehyde (0.073 g, 0.50 mmol) in ethanol (2.0 mL), piperidine (0.054 mL, 0.55 mmol) was added at ambient temperature. The mixture was refluxed for 12 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol and diisopropyl ether then dried to afford the titled compound (0.099 g, y. 48%).

¹H NMR (DMSO-d₆) δ (ppm) 12.31 (br. s, 1H), 10.65 (s, 1H), 8.22 (dd, J=1.38, 4.64 Hz, 1H), 7.70-7.83 (m, 4H), 7.51-7.64 (m, 2H), 6.92 (s, 1H), 6.81 (dd, J=4.64, 7.91 Hz, 1H), 4.29 (q, J=7.11 Hz, 2H), 1.30 (t, J=7.03 Hz, 3H); LCMS (m/z): 409.8 [M+H]⁺.

Example 16 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{[4-(dimethylamino)phenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate hydrochloride

To a solution of ethyl 2-{[4-(dimethylamino)phenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate (0.073 g, 0.20 mmol) which similarly prepared according to the procedure described in the Example 2, First step and 7-azaindole-3-carboxaldehyde (0.029 g, 0.20 mmol) in ethanol (1.5 mL), 2M hydrochloric acid in ethanol (0.33 mL, 0.66 mmol) was added at ambient temperature. The mixture was refluxed for 1 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol and diisopropyl ether then dried to afford the titled compound (0.034 g, y. 35%).

¹H NMR (DMSO-d₆) δ (ppm) 12.51 (br. s, 1H), 10.47 (br. s, 1H), 8.23-8.33 (m, 1H), 8.10 (d, J=7.78 Hz, 1H), 7.81 (s, 1H), 7.50 (d, J=8.03 Hz, 2H), 7.16 (br. s, 2H), 6.95 (dd, J=4.77, 7.78 Hz, 1H), 6.90 (s, 1H), 4.40-5.20 (m, 1H), 4.27 (q, J=7.03 Hz, 2H), 3.08 (s, 6H), 1.29 (t, J=7.15 Hz, 3H); LCMS (m/z): 419.0 [M+H]⁺.

Example 17 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(diethylamino)-4-oxo-4,5-dihydrofuran-3-carboxylate hydrochloride

To a solution of ethyl 2-(diethylamino)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.034 g, 0.15 mmol) which similarly prepared according to the procedure described in the Example 4, First step and 7-azaindole-3-carboxaldehyde (0.022 g, 0.15 mmol) in ethanol (1.0 mL), 2M hydrochloric acid in ethanol (0.16 mL, 0.32 mmol) was added at ambient temperature. The mixture was refluxed for 3 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol and diisopropyl ether then dried to afford the titled compound (0.023 g, y. 38%).

¹H NMR (DMSO-d₆) δ (ppm) 12.36 (br. s, 1H), 8.41 (d, J=7.78 Hz, 1H), 8.33 (dd, J=1.25, 4.77 Hz, 1H), 7.93 (d, J=2.26 Hz, 1H), 7.22 (dd, J=4.77, 7.78 Hz, 1H), 6.84 (s, 1H), 4.18 (q, J=7.11 Hz, 2H), 3.50-3.84 (m, 5H), 1.11-1.38 (m, 9H); LCMS (m/z): 356.0 [M+H]⁺.

Example 18 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(cyclohexylamino)-4-oxo-4,5-dihydrofuran-3-carboxylate hydrochloride

To a solution of ethyl 2-(cyclohexylamino)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.019 g, 0.075 mmol) which similarly prepared according to the procedure described in the Example 2, First step and 7-azaindole-3-carboxaldehyde (0.011 g, 0.075 mmol) in ethanol (1.0 mL), 2M hydrochloric acid in ethanol (0.079 mL, 0.16 mmol) was added at ambient temperature. The mixture was refluxed for 4 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol and diisopropyl ether then dried to afford the titled compound (0.0070 g, y. 22%).

¹H NMR (DMSO-d₆) δ (ppm) 12.40 (br. s, 1H), 8.53 (d, J=8.53 Hz, 1H), 8.46 (d, J=7.78 Hz, 1H), 8.34 (dd, J=1.26, 4.52 Hz, 1H), 7.99 (d, J=2.26 Hz, 1H), 7.22 (dd, J=4.64, 7.91 Hz, 1H), 6.90 (s, 1H), 4.21 (q, J=7.03 Hz, 2H), 3.91-4.09 (m, 1H), 1.98 (d, J=9.79 Hz, 2H), 1.79 (d, J=13.05 Hz, 2H), 1.55-1.70 (m, 3H), 1.35-1.49 (m, 2H), 1.16-1.29 (m, 4H); LCMS (m/z): 382.4 [M+H]⁺.

Example 19 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-bromophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-[(4-bromophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.15 g, 0.46 mmol) which similarly prepared according to the procedure described in the Example 4, First step and 7-azaindole-3-carboxaldehyde (0.067 g, 0.46 mmol) in ethanol (5.0 mL), piperidine (0.85 mL, 0.92 mmol) was added at ambient temperature. The mixture was refluxed for 24 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol then dried to afford the titled compound (0.075 g, y. 36%).

¹H NMR (DMSO-d₆) δ (ppm) 12.34 (br. s, 1H), 10.61 (br. s, 1H), 8.26 (d, J=3.42 Hz, 1H), 7.95 (d, J=7.34 Hz, 1H), 7.84 (br. s, 1H), 7.72 (d, J=8.31 Hz, 2H), 7.57 (d, J=8.31 Hz, 2H), 6.77-7.05 (m, 2H), 4.07-4.42 (m, 2H), 1.29 (t, J=6.85 Hz, 3H); LCMS (m/z): 382.4 [M+H]⁺.

Example 20 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-4-oxo-2-(p-tolylamino)-4,5-dihydrofuran-3-carboxylate

The titled compound was similarly prepared according to the procedure described in the Example 19.

¹H NMR (DMSO-d₆) δ (ppm) 12.30 (br. s, 1H), 10.51 (br. s, 1H), 8.24 (d, J=3.42 Hz, 1H), 7.97 (d, J=7.34 Hz, 1H), 7.80 (br. s, 1H), 7.46 (d, J=7.82 Hz, 2H), 7.34 (d, J=8.31 Hz, 2H), 6.74-7.02 (m, 2H), 4.14-4.37 (m, 2H), 2.44 (s, 3H), 1.29 (t, J=6.85 Hz, 3H); LCMS (m/z): 390.6 [M+H]⁺.

Example 21 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 19.

¹H NMR (DMSO-d₆) δ (ppm) 12.28 (br. s, 1H), 10.57 (br. s, 1H), 8.24 (br. s, 1H), 7.95 (d, J=7.82 Hz, 1H), 7.75 (br. s, 1H), 7.60-7.68 (m, 2H), 7.38 (t, J=8.31 Hz, 2H), 6.85-6.93 (m, 2H), 4.11-4.50 (m, 2H), 1.29 (t, J=6.85 Hz, 3H); LCMS (m/z): 394.2 [M+H]⁺.

Example 22 5-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-4-oxo-2-(phenylamino)-4,5-dihydrofuran-3-carboxylic acid

To a solution of the compound (0.050 g, 0.13 mmol) of Example 1 in ethanol (1.0 mL), 50% potassium hydroxide solution (0.5 mL, 0.13 mmol) was added at ambient temperature. The mixture was refluxed for 1 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol. The crude material was dissolved in water (0.5 mL) and tetrahydrofuran (0.5 mL), then 2M hydrochloric acid (0.023 mL, 0.046 mmol) was added and the mixture was stirred for 30 min. The precipitate was collected by filtration, washed with water and diethyl ether then dried to afford the titled compound (0.012 g, y. 26%).

¹H NMR (DMSO-d₆) δ (ppm) 12.11 (br. s, 2H), 8.21 (dd, J=1.25, 4.52 Hz, 1H), 8.06 (d, J=7.78 Hz, 1H), 7.73 (d, J=2.26 Hz, 1H), 7.38-7.49 (m, 2H), 7.32 (d, J=7.53 Hz, 2H), 7.19-7.27 (m, 1H), 6.91 (dd, J=4.64, 7.91 Hz, 1H), 6.66 (s, 1H); LCMS (m/z): 347.9 [M+H]⁺.

Example 23 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(2-hydroxyphenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-[(2-hydroxyphenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.050 g, 0.19 mmol) which similarly prepared according to the procedure described in the Example 4 and 7-azaindole-3-carboxaldehyde (0.023 g, 0.16 mmol) in ethanol (1.0 mL), piperidine (0.0010 mL, 0.010 mmol) was added at ambient temperature. The mixture was refluxed for 2 days. The precipitate was collected by filtration, washed with hot ethanol. The solid was washed hexane then dried to afford the titled compound (0.008 g, y. 11%).

¹H NMR (DMSO-d₆) δ (ppm) 12.33 (br. s, 1H), 10.35 (s, 1H), 10.30 (s, 1H), 8.25 (dd, J=1.38, 4.64 Hz, 1H), 8.06 (d, J=7.03 Hz, 1H), 7.87 (d, J=2.76 Hz, 1H), 7.58 (d, J=7.03 Hz, 1H), 7.24-7.33 (m, 1H), 7.07 (dd, J=1.00, 8.28 Hz, 1H), 6.90-7.01 (m, 3H), 4.28 (q, J=7.03 Hz, 2H), 1.30 (t, J=7.15 Hz, 3H); LCMS (m/z): 392.0 [M+H]⁺.

Example 24 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(2,4-dimethoxyphenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-[(2,4-dimethoxyphenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.35 g, 1.1 mmol) which similarly prepared according to the procedure described in the Example 4, First step and 7-azaindole-3-carboxaldehyde (0.12 g, 1.1 mmol) in ethanol (10 mL), piperidine (0.5 mL, 4.6 mmol) was added at ambient temperature. The mixture was refluxed for 16 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol then dried to afford the titled compound (0.13 g, y. 26%).

¹H NMR (DMSO-d₆) δ (ppm) 12.31 (br. s, 1H), 10.14 (br. s, 1H), 8.24 (br. s, 1H), 7.94 (d, J=7.82 Hz, 1H), 7.80 (br. s, 1H), 7.51 (d, J=8.80 Hz, 1H), 6.78-6.93 (m, 3H), 6.68 (d, J=8.31 Hz, 1H), 4.26 (d, J=6.85 Hz, 2H), 3.89 (s, 3H), 3.81 (s, 3H), 1.29 (t, J=6.60 Hz, 3H); LCMS (m/z): 436.2 [M+H]⁺.

Example 25 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-carbamoylphenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 24.

¹H NMR (DMSO-d₆) δ (ppm) 12.34 (br. s, 1H), 10.67 (br. s, 1H), 8.24 (br. s, 1H), 7.95-8.13 (m, 4H), 7.84 (br. s, 1H), 7.67 (d, J=7.34 Hz, 2H), 7.49 (br. s, 1H), 6.75-7.08 (m, 2H), 4.28 (d, J=6.85 Hz, 2H), 1.30 (t, J=6.36 Hz, 3H); LCMS (m/z): 419.0 [M+H]⁺.

Example 26 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(2,4-dimethylphenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 24.

¹H NMR (DMSO-d₆) δ (ppm) 12.26 (br. s, 1H), 10.35 (br. s, 1H), 8.21 (d, J=3.91 Hz, 1H), 7.65-7.80 (m, 2H), 7.39 (d, J=8.31 Hz, 1H), 7.27 (s, 1H), 7.20 (d, J=7.82 Hz, 1H), 6.84 (s, 1H), 6.73 (dd, J=4.40, 7.83 Hz, 1H), 4.27 (q, J=7.01 Hz, 2H), 2.44 (s, 3H), 2.23 (s, 3H), 1.30 (t, J=6.85 Hz, 3H); LCMS (m/z): 404.0 [M+H]⁺.

Example 27 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-4-oxo-2-(3-pyridinylamino)-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 24.

¹H NMR (DMSO-d₆) δ (ppm) 12.33 (br. s, 1H), 10.72 (br. s, 1H), 8.82 (br. s, 1H), 8.65 (br. s., 1H), 8.24 (br. s, 1H), 7.90-8.11 (m, 2H), 7.73 (br. s, 1H), 7.54-7.62 (m, 1H), 6.87-6.97 (m, 2H), 4.28 (d, J=6.85 Hz, 2H), 1.30 (t, J=6.36 Hz, 3H); LCMS (m/z): 377.6 [M+H]⁺.

Example 28 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(3,4-dimethoxyphenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 24.

¹H NMR (DMSO-d₆) δ (ppm) 12.33 (br. s, 1H), 10.44 (s, 1H), 8.24 (d, J=3.91 Hz, 1H), 7.96 (d, J=7.82 Hz, 1H), 7.82 (br. s, 1H), 7.24 (s, 1H), 7.06-7.14 (m, 2H), 6.78-6.94 (m, 2H), 4.27 (q, J=7.01 Hz, 2H), 3.87 (s, 3H), 3.71 (s, 3H), 1.29 (t, J=7.09 Hz, 3H); LCMS (m/z): 436.5 [M+H]⁺.

Example 29 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{[2-(2-hydroxyethoxy)phenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate

First Step

A solution of ethyl 2-[(2-hydroxyphenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.10 g, 0.38 mmol) which similarly prepared according to the procedure described in the Example 4, First step, 2-chloroethanol (0.038 mL, 0.57 mmol) and potassium carbonate (0.11 g, 0.80 mmol) in N,N-dimethylformamide (2.0 mL) was stirred at 90° C. for 2 days. Cooled to ambient temperature, ethyl acetate was added to the reaction mixture, the organic layer was washed with aqueous 5% citric acid solution, water and brine, dried over magnesium sulfate and concentrated. To the residue, tert-butyl methyl ether was added to precipitate the product. The precipitate was collected by filtration, washed with hexane then dried to afford ethyl 2-{[2-(2-hydroxyethoxy)phenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate as solid (0.041 g, y. 35%).

¹H NMR (DMSO-d₆) δ (ppm) 10.40 (s, 1H), 7.66 (d, J=7.53 Hz, 1H), 7.16-7.25 (m, 2H), 6.97-7.05 (m, 1H), 4.82 (br. s, 1H), 4.75 (s, 2H), 4.24 (q, J=7.03 Hz, 2H), 4.15 (t, J=5.14 Hz, 2H), 3.72-3.80 (m, 2H), 1.26 (t, J=7.15 Hz, 3H); LCMS (m/z): 308.0 [M+H]⁺.

Second Step

To a solution of ethyl 2-{[2-(2-hydroxyethoxy)phenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate (0.021 g, 0.068 mmol) and 7-azaindole-3-carboxaldehyde (0.0087 g, 0.060 mmol) in ethanol (0.5 mL), 2M hydrochloric acid in ethanol (0.030 mL, 0.059 mmol) was added at ambient temperature. The mixture was refluxed for 4 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol and hexane then dried to afford the titled compound as solid (0.021 g, y. 72%).

¹H NMR (DMSO-d₆) δ (ppm) 12.36 (br. s, 1H), 10.33 (s, 1H), 8.24-8.29 (m, 1H), 8.07 (d, J=6.78 Hz, 1H), 7.85 (s, 1H), 7.65-7.70 (m, 1H), 7.38-7.46 (m, 1H), 7.29 (d, J=7.78 Hz, 1H), 7.11 (t, J=7.65 Hz, 1H), 6.92-6.99 (m, 2H), 4.41 (br. s, 1H), 4.28 (q, J=7.20 Hz, 2H), 4.10 (t, J=5.14 Hz, 2H), 3.65 (t, J=5.02 Hz, 2H), 1.30 (t, J=7.03 Hz, 3H); LCMS (m/z): 436 [M+H]⁺.

Example 30 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{[2-(2-morpholinoethoxy)phenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-{[2-(2-morpholinoethoxy)phenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate (0.021 g, 0.054 mmol) which similarly prepared according to the procedure described in the Example 29, First step and 7-azaindole-3-carboxaldehyde (0.0070 g, 0.048 mmol) in ethanol (0.5 mL), 2M hydrochloric acid in ethanol (0.024 mL, 0.048 mmol) was added at ambient temperature. The mixture was refluxed for 4 h. The reaction mixture was cooled with ice bath. Aqueous 2M sodium hydroxide solution (0.024 mL, 0.048 mmol) was added dropwise to neutralize and the mixture was stirred for 1 h. The precipitate was collected by filtration then purified by preparative HPLC to afford the titled compound as solid (0.0054 g, y. 21%).

¹H NMR (DMSO-d₆) δ (ppm) 12.31 (br. s, 1H), 10.35 (br. s, 1H), 8.24 (dd, J=1.25, 4.52 Hz, 1H), 7.94 (d, J=7.78 Hz, 1H), 7.83 (d, J=2.01 Hz, 1H), 7.62 (d, J=7.03 Hz, 1H), 7.42-7.50 (m, 1H), 7.28 (d, J=7.78 Hz, 1H), 7.12 (t, J=7.28 Hz, 1H), 6.92 (s, 1H), 6.88 (dd, J=4.64, 7.91 Hz, 1H), 4.27 (q, J=7.03 Hz, 2H), 4.14 (t, J=5.14 Hz, 2H), 3.36-3.42 (m, 4H), 2.59 (t, J=5.02 Hz, 2H), 2.25-2.35 (m, 4H), 1.30 (t, J=7.03 Hz, 3H); LCMS (m/z): 505.4 [M+H]⁺.

Example 31 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(2-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-[(2-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.10 g, 0.38 mmol) which similarly prepared according to the procedure described in the Example 4, First step and 7-azaindole-3-carboxaldehyde (0.050 g, 0.34 mmol) in ethanol (2.0 mL), piperidine (0.0034 mL, 0.034 mmol) was added at ambient temperature. The mixture was refluxed for 36 h. The precipitate was collected by filtration, washed with hot ethanol. The solid was washed with hexane then dried to afford the titled compound as solid (0.037 g, y. 27%).

¹H NMR (DMSO-d₆) δ (ppm) 12.31 (br. s, 1H), 10.55 (s, 1H), 8.22 (dd, J=1.38, 4.64 Hz, 1H), 7.84 (d, J=7.78 Hz, 1H), 7.67-7.77 (m, 2H), 7.55-7.63 (m, 1H), 7.45-7.53 (m, 1H), 7.37-7.44 (m, 1H), 6.92 (s, 1H), 6.83 (dd, J=4.64, 7.91 Hz, 1H), 4.28 (q, J=7.03 Hz, 2H), 1.30 (t, J=7.03 Hz, 3H); LCMS (m/z): 393.9 [M+H]⁺.

Example 32 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-4-oxo-2-(o-tolylamino)-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 4-oxo-2-(o-tolylamino)-4,5-dihydrofuran-3-carboxylate (0.098 g, 0.38 mmol) which similarly prepared according to the procedure described in the Example 4, First step and 7-azaindole-3-carboxaldehyde (0.050 g, 0.34 mmol) in ethanol (2.0 mL), piperidine (0.0034 mL, 0.034 mmol) was added at ambient temperature. The mixture was refluxed for 32 h. The precipitate was collected by filtration, washed with hot ethanol. The solid was washed with hexane then dried to afford the titled compound as solid (0.019 g, y. 27%).

¹H NMR (DMSO-d₆) δ (ppm) 12.26 (br. s, 1H), 10.43 (s, 1H), 8.18-8.22 (m, 1H), 7.69-7.75 (m, 2H), 7.54 (d, J=7.78 Hz, 1H), 7.45-7.49 (m, 2H), 7.37-7.44 (m, 1H), 6.85 (s, 1H), 6.78 (dd, J=4.64, 7.91 Hz, 1H), 4.28 (q, J=7.19 Hz, 2H), 2.28 (s, 3H), 1.30 (t, J=7.03 Hz, 3H); LCMS (m/z): 390 [M+H]⁺.

Example 33 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-({2-([2-(dimethylamino)ethoxy]phenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-({2-[2-(dimethylamino)ethoxy]phenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.010 g, 0.031 mmol) which similarly prepared according to the procedure described in the Example 29, First step and 7-azaindole-3-carboxaldehyde (0.0042 g, 0.029 mmol) in 2-propanol (0.3 mL), piperidine (0.00028 mL, 0.0028 mmol) was added at ambient temperature. The mixture was refluxed for 24 h. The precipitate was collected by filtration, washed with hot ethanol. The solid was washed with hexane then dried to afford the titled compound as solid (0.0029 g, y. 22%).

¹H NMR (DMSO-d₆) δ (ppm) 12.24 (br. s, 1H), 8.23 (dd, J=1.26, 4.52 Hz, 1H), 7.98 (d, J=7.53 Hz, 1H), 7.79 (s, 1H), 7.58 (d, J=7.28 Hz, 1H), 7.36-7.45 (m, 1H), 7.28 (d, J=7.53 Hz, 1H), 7.13 (t, J=7.53 Hz, 1H), 6.89 (dd, J=4.64, 7.91 Hz, 1H), 6.83 (s, 1H), 4.26 (q, J=7.19 Hz, 2H), 4.15 (t, J=5.52 Hz, 2H), 2.55-2.65 (m, 2H), 2.19 (s, 6H), 1.29 (t, J=7.03 Hz, 3H); LCMS (m/z): 463 [M+H]⁺.

Example 34 Methyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-4-oxo-2-piperidino-4,5-dihydrofuran-3-carboxylate

To a solution of methyl 4-oxo-2-(phenylamino)-4,5-dihydrofuran-3-carboxylate (0.047 g, 0.20 mmol) which similarly prepared according to the procedure described in the Example 2, First step using methyl 4-chloroacetoacetate and phenyl isocyanate, and 7-azaindole-3-carboxaldehyde (0.029 g, 0.20 mmol) in methanol (1.0 mL), piperidine (0.022 mL, 0.22 mmol) was added at ambient temperature. The mixture was refluxed for 5 days. Cooled to ambient temperature, the precipitate was collected by filtration, then purified by preparative HPLC, collected former fraction to afford the titled compound as solid (0.0075 g, y. 11%).

¹H NMR (DMSO-d₆) δ (ppm) 12.30 (br. s, 1H), 8.35-8.43 (m, 1H), 8.31 (dd, J=1.38, 4.64 Hz, 1H), 7.97 (d, J=2.51 Hz, 1H), 7.19 (dd, J=4.64, 7.91 Hz, 1H), 6.87 (s, 1H), 3.70-3.82 (m, 4H), 3.68 (s, 3H), 1.64-1.80 (m, 6H); LCMS (m/z): 354.0 [M+H]⁺

Example 35 Methyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-4-oxo-2-(phenylamino)-4,5-dihydrofuran-3-carboxylate

To a solution of methyl 4-oxo-2-(phenylamino)-4,5-dihydrofuran-3-carboxylate (0.047 g, 0.20 mmol) which similarly prepared according to the procedure described in the Example 2, First step using methyl 4-chloroacetoacetate and phenyl isocyanate, and 7-azaindole-3-carboxaldehyde (0.029 g, 0.20 mmol) in 2-propanol (1.0 mL), piperidine (0.022 mL, 0.22 mmol) was added at ambient temperature. The mixture was refluxed for 5 days. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol and diisopropyl ether then dried to afford the titled compound as solid (0.031 g, y. 43%).

¹H NMR (DMSO-d₆) δ (ppm) 12.29 (br. s, 1H), 10.59 (s, 1H), 8.23 (dd, J=1.38, 4.64 Hz, 1H), 7.98 (d, J=7.53 Hz, 1H), 7.77 (d, J=2.51 Hz, 1H), 7.52-7.64 (m, 4H), 7.44-7.51 (m, 1H), 6.93 (s, 1H), 6.89 (dd, J=4.64, 7.91 Hz, 1H), 3.77 (s, 3H); LCMS (m/z): 361.9 [M+H]⁺.

Example 36 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(4-methylpiperazinyl)-4-oxo-4,5-dihydrofuran-3-carboxylate hydrochloride

To a solution of ethyl 2-(4-methylpiperazinyl)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.038 g, 0.15 mmol) which similarly prepared according to the procedure described in the Example 4, First step and 7-azaindole-3-carboxaldehyde (0.022 g, 0.15 mmol) in ethanol (1.0 mL), 2M hydrochloric acid in methanol (0.17 mL, 0.34 mmol) was added at ambient temperature. The mixture was refluxed for 40 min. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol then dried to afford the titled compound as solid (0.0069 g, y. 9%).

¹H NMR (DMSO-d₆) δ (ppm) 12.32-12.49 (m, 1H), 10.69 (br. s, 1H), 8.39 (d, J=8.03 Hz, 1H), 8.29-8.36 (m, 1H), 8.08 (d, J=2.76 Hz, 1H), 7.22 (dd, J=4.64, 7.91 Hz, 1H), 6.95 (s, 1H), 4.19 (q, J=7.19 Hz, 2H), 3.57-3.75 (m, 4H), 3.33-3.40 (m, 4H), 2.87 (br. s., 3H), 1.16-1.34 (m, 3H); LCMS (m/z): 383.0 [M+H]⁺.

Example 37 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-isopropylphenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-[(4-isopropylphenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.26 g, 0.90 mmol) which similarly prepared according to the procedure described in the Example 4, First step and 7-azaindole-3-carboxaldehyde (0.13 g, 0.90 mmol) in ethanol (6.0 mL), piperidine (0.045 mL, 0.45 mmol) was added at ambient temperature. The mixture was refluxed for 16 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol then dried to afford the titled compound as solid (0.13 g, y. 35%).

¹H NMR (DMSO-d₆) δ (ppm) 12.35 (br. s, 1H), 10.50 (br. s, 1H), 8.24 (d, J=3.42 Hz, 1H), 8.04 (d, J=7.82 Hz, 1H), 7.82 (br. s, 1H), 7.33-7.58 (m, 4H), 6.82-6.96 (m, 2H), 4.08-4.44 (m, 2H), 2.86-3.17 (m, 1H), 1.31 (s, 3H), 1.29 (s, 6H); LCMS (m/z): 418.0 [M+H]⁺.

Example 38 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-4-oxo-2-(5-pyrimidinylamino)-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 37.

¹H NMR (DMSO-d₆) δ (ppm) 12.34 (br. s, 1H), 10.80 (br. s, 1H), 9.19 (s, 1H), 9.02-9.12 (m, 2H), 8.25 (d, J=4.40 Hz, 1H), 8.03 (d, J=7.82 Hz, 1H), 7.71 (br. s, 1H), 6.86-7.00 (m, 2H), 4.28 (q, J=6.85 Hz, 2H), 1.30 (t, J=6.00 Hz, 3H); LCMS (m/z): 378.2 [M+H]⁺.

Example 39 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(1,1′-biphenyl)-4-ylamino]-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 37.

¹H NMR (DMSO-d₆) δ (ppm) 12.34 (br. s, 1H), 10.63 (br. s, 1H), 8.20 (br. s, 1H), 8.09 (d, J=6.85 Hz, 1H), 7.75-7.93 (m, 5H), 7.62-7.74 (m, 2H), 7.50-7.58 (m, 2H), 7.39-7.48 (m, 1H), 6.94 (br. s, 1H), 6.83-6.91 (m, 1H), 4.29 (d, J=6.36 Hz, 2H), 1.31 (br. s, 3H); LCMS (m/z): 452.6 [M+H]⁺.

Example 40 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-nitrophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 37.

¹H NMR (DMSO-d₆) δ (ppm) 12.39 (br. s, 1H), 10.89 (br. s, 1H), 8.35 (d, J=8.80 Hz, 2H), 8.27 (d, J=3.91 Hz, 1H), 8.17 (d, J=7.82 Hz, 1H), 7.88 (d, J=8.80 Hz, 2H), 7.84 (d, J=2.45 Hz, 1H), 7.03 (s, 1H), 6.98 (dd, J=4.65, 8.07 Hz, 1H), 4.28 (q, J=7.17 Hz, 2H), 1.29 (t, J=7.09 Hz, 3H); LCMS (m/z): 421.4 [M+H]⁺.

Example 41 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-aminophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate hydrochloride

First Step

To a solution of ethyl 2-({4-[(tert-butoxycarbonyl)amino]phenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.32 g, 0.88 mmol) which similarly prepared according to the procedure described in the Example 4, First step and 7-azaindole-3-carboxaldehyde (0.13 g, 0.88 mmol) in ethanol (5.0 mL), piperidine (0.18 mL, 1.8 mmol) was added at ambient temperature. The mixture was refluxed for 16 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol then dried to afford ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-({4-[(tert-butoxycarbonyl)amino]phenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate as solid (0.095 g, y. 21%).

¹H NMR (DMSO-d₆) δ 12.30 (br. s, 1H), 10.44 (br. s, 1H), 9.60 (s, 1H), 8.20 (d, J=3.76 Hz, 1H), 7.91 (d, J=7.72 Hz, 1H), 7.82 (s, 1H), 7.60 (d, J=8.40 Hz, 2H), 7.45 (d, J=8.56 Hz, 2H), 6.82-6.92 (m, 2H), 4.26 (q, J=6.86 Hz, 2H), 1.53 (s, 9H), 1.29 (t, J=6.90 Hz, 3H); LCMS (m/z): 491.4 [M+H]⁺.

Second Step

A solution of ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{[4-(tert-butoxycarbonyl)aminophenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate (0.090 g, 0.18 mmol) in 4M hydrochloric acid in dioxane (3.0 mL) was stirred at ambient temperature for 2 h. The solvent was removed under reduced pressure, and diethyl ether was added to precipitate the product. The precipitate was collected by filtration, washed with diethyl ether then dried to afford the titled compound as solid (0.066 g, y. 92%).

¹H NMR (DMSO-d₆) δ (ppm) 12.44 (br. s, 1H), 10.61 (br. s, 1H), 8.26 (d, J=3.91 Hz, 1H), 8.02 (d, J=7.82 Hz, 1H), 7.79 (s, 1H), 7.61 (d, J=8.31 Hz, 2H), 7.40 (d, J=7.34 Hz, 2H), 7.01 (dd, J=4.89, 7.83 Hz, 1H), 6.92 (s, 1H), 4.68 (br. s, 3H), 4.27 (q, J=7.01 Hz, 2H), 1.29 (t, J=6.85 Hz, 3H); LCMS (m/z): 391.2 [M+H]⁺.

Example 42 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-morpholinophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 41, First step.

¹H NMR (DMSO-d₆) δ (ppm) 12.31 (br. s, 1H), 10.39 (br. s, 1H), 8.24 (br. s, 1H), 7.99 (d, J=7.34 Hz, 1H), 7.79 (br. s, 1H), 7.42 (d, J=8.31 Hz, 2H), 7.07 (d, J=8.31 Hz, 2H), 6.81-6.96 (m, 2H), 4.26 (d, J=6.85 Hz, 2H), 3.28-3.40 (m, 4H), 3.15-3.28 (m, 4H), 1.29 (t, J=6.85 Hz, 3H); LCMS (m/z): 461.4 [M+H]⁺.

Example 43 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-4-oxo-2-(4-pyridinylamino)-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 41, First step.

¹H NMR (DMSO-d₆) δ (ppm) 12.30 (br. s, 1H), 11.18 (br. s, 1H), 8.59 (d, J=3.91 Hz, 2H), 8.28 (d, J=3.91 Hz, 1H), 8.19 (d, J=7.34 Hz, 1H), 7.88 (br. s, 1H), 7.55 (br. s, 2H), 7.02 (dd, J=4.40, 7.83 Hz, 1H), 6.93 (br. s, 1H), 4.23 (d, J=6.85 Hz, 2H), 1.26 (t, J=6.85 Hz, 3H); LCMS (m/z): 377.6 [M+H]⁺.

Example 44 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-4-oxo-2-(6-quinolinylamino)-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 41, First step.

¹H NMR (DMSO-d₆) δ (ppm) 12.29 (br. s, 1H), 10.82 (s, 1H), 9.01 (d, J=3.42 Hz, 1H), 8.39 (d, J=8.31 Hz, 1H), 8.23 (br. s, 1H), 8.17 (d, J=8.80 Hz, 1H), 8.11 (d, J=3.91 Hz, 1H), 8.00 (d, J=8.80 Hz, 1H), 7.88 (d, J=7.34 Hz, 1H), 7.83 (br. s, 1H), 7.62 (dd, J=4.16, 8.07 Hz, 1H), 6.94 (s, 1H), 6.26-6.51 (m, 1H), 4.30 (q, J=6.85 Hz, 2H), 1.32 (t, J=6.85 Hz, 3H); LCMS (m/z): 427.4 [M+H]⁺.

Example 45 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-fluorobenzyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 41, First step.

¹H NMR (DMSO-d₆) δ (ppm) 12.31 (br. s, 1H), 9.51 (br. s, 1H), 8.22-8.37 (m, 2H), 7.86 (br. s, 1H), 7.47 (t, J=6.30 Hz, 2H), 7.22 (t, J=8.31 Hz, 2H), 7.05-7.15 (m, 1H), 6.87 (s, 1H), 4.84 (d, J=5.87 Hz, 2H), 4.23 (q, J=6.52 Hz, 2H), 1.27 (t, J=6.85 Hz, 3H); LCMS (m/z): 408.2 [M+H]⁺.

Example 46 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(2-fluorobenzyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 41, First step.

¹H NMR (DMSO-d₆) δ (ppm) 12.35 (br. s, 1H), 9.44 (br. s, 1H), 8.15-8.42 (m, 2H), 7.86 (s, 1H), 7.45 (t, J=7.34 Hz, 1H), 7.33-7.40 (m, 1H), 7.18-7.32 (m, 2H), 7.07 (dd, J=5.14, 7.58 Hz, 1H), 6.87 (s, 1H), 4.91 (s, 2H), 4.23 (q, J=7.17 Hz, 2H), 1.27 (t, J=6.85 Hz, 3H); LCMS (m/z): 408.0 [M+H]⁺.

Example 47 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-fluorophenyl)-N-methylamino]-4-oxo-4,5-dihydrofuran-3-carboxylate hydrochloride

First Step

A solution of ethyl 2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.10 g, 0.38 mmol) which similarly prepared according to the procedure described in the Example 4, First step, potassium carbonate (0.063 g, 0.45 mmol) and methyliodide (0.026 mL, 0.42 mmol) in anhydrous N,N-dimethylformamide (2.0 mL) was stirred at ambient temperature for 12 h. The reaction mixture was diluted with water then extracted with ethyl acetate for 3 times. The organic layer was dried over sodium sulfate and concentrated to afford ethyl 2-[(4-fluorophenyl)-N-methylamino]-4-oxo-4,5-dihydrofuran-3-carboxylate as solid (0.13 g).

¹H NMR (DMSO-d₆) δ (ppm) 7.38-7.47 (m, 2H), 7.21-7.32 (m, 2H), 4.65 (s, 2H), 3.60 (q, J=7.03 Hz, 2H), 3.43 (s, 3H), 0.96 (t, J=7.15 Hz, 3H); LCMS (m/z): 279.9 [M+H]⁺.

Second Step

To a solution of ethyl 2-[(4-fluorophenyl)-N-methylamino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.050 g, 0.18 mmol) and 7-azaindole-3-carboxaldehyde (0.026 g, 0.18 mmol) in ethanol (1.0 mL), 2M hydrochloric acid in ethanol (0.19 mL, 0.38 mmol) was added at ambient temperature. The mixture was refluxed for 2 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol and diisopropyl ether then dried to afford the titled compound as solid (0.011 g, y. 13%).

¹H NMR (DMSO-d₆) δ (ppm) 12.38 (br. s, 1H), 8.28 (dd, J=1.25, 4.77 Hz, 1H), 8.12 (d, J=7.78 Hz, 1H), 7.81 (d, J=2.01 Hz, 1H), 7.53-7.62 (m, 2H), 7.31-7.38 (m, 2H), 7.05 (dd, J=4.77, 7.78 Hz, 1H), 6.90 (s, 1H), 3.80 (q, J=7.03 Hz, 2H), 3.63 (s, 3H), 1.01-1.10 (m, 3H); LCMS (m/z): 407.9 [M+H]⁺.

Example 48 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(2-methoxyethyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-[(2-methoxyethyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.50 g, 2.2 mmol) which similarly prepared according to the procedure described in the Example 4, First step and 7-azaindole-3-carboxaldehyde (0.32 g, 2.2 mmol) in ethanol (3.0 mL), piperidine (5 drops) was added at ambient temperature. The mixture was refluxed for 16 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol then dried to afford the titled compound as solid.

¹H NMR (DMSO-d₆) δ (ppm) 12.34 (br. s, 1H), 8.89 (br. s, 1H), 8.43 (d, J=7.34 Hz, 1H), 8.31 (d, J=3.91 Hz, 1H), 8.03 (s, 1H), 7.19 (dd, J=4.40, 7.83 Hz, 1H), 6.90 (s, 1H), 4.21 (q, J=7.17 Hz, 2H), 3.79 (d, J=4.40 Hz, 2H), 3.62 (t, J=5.14 Hz, 2H), 3.28 (s, 3H), 1.26 (t, J=7.09 Hz, 3H); LCMS (m/z): 358.4 [M+H]⁺.

Example 49 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-4-oxo-2-[(2-piperidinoethyl)amino]-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 48.

¹H NMR (DMSO-d₆) δ (ppm) 12.35 (br. s, 1H), 8.83 (br. s, 1H), 8.38-8.47 (m, 1H), 8.31 (br. s, 1H), 8.02 (br. s, 1H), 7.20 (br. s, 1H), 6.88 (br. s, 1H), 4.12-4.28 (m, 2H), 3.67-3.80 (m, 2H), 2.25-2.70 (m, 6H), 1.32-1.53 (m, 6H), 1.18-1.32 (m, 3H); LCMS (m/z): 411.5 [M+H]⁺.

Example 50 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(benzyl-N-methylamino)-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 48.

¹H NMR (DMSO-d₆) δ (ppm) 12.28 (br. s, 1H), 8.24-8.39 (m, 2H), 7.88 (br. s, 1H), 7.29-7.47 (m, 5H), 7.10 (br. s, 1H), 6.87 (s, 1H), 5.01 (s, 2H), 4.14 (d, J=6.85 Hz, 2H), 3.25 (br. s, 3H), 1.19 (t, J=6.36 Hz, 3H); LCMS (m/z): 404.6 [M+H]⁺.

Example 51 Methyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of methyl 2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.25 g, 1.0 mmol) which similarly prepared according to the procedure described in the Example 2, First step using methyl 4-chloroacetoacetate and 4-fluorophenyl isocyanate, and 7-azaindole-3-carboxaldehyde (0.15 g, 1.0 mmol) in 2-propanol (5.0 mL), L-proline (0.023 g, 0.20 mmol) was added at ambient temperature. The mixture was refluxed for 2 days. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol and diisopropyl ether then dried to afford the titled compound as solid (0.26 g, y. 68%).

¹H NMR (DMSO-d₆) δ (ppm) 12.28 (br. s, 1H), 10.58 (s, 1H), 8.25 (dd, J=1.38, 4.64 Hz, 1H), 7.96 (d, J=8.03 Hz, 1H), 7.74 (d, J=2.51 Hz, 1H), 7.59-7.69 (m, 2H), 7.36-7.43 (m, 2H), 6.92 (s, 1H), 6.89 (dd, J=4.77, 8.03 Hz, 1H), 3.76 (s, 3H); LCMS (m/z): 379.8 [M+H]⁺.

Example 52 n-Butyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

First Step

Under a nitrogen atmosphere, a solution of methyl 2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.13 g, 0.50 mmol) which similarly prepared according to the procedure described in the Example 2, First step using methyl 4-chloroacetoacetate and 4-fluorophenyl isocyanate, and zinc cluster catalyst (Zn₄(OCOCF₃)₆O) (0.0062 g, 0.0065 mmol) in 1-butanol (2.0 mL) was stirred at 80° C. for 3 days then stirred at ambient temperature for further 3 days. The precipitate was collected by filtration, washed with 2-propanol and diisopropyl ether then dried to afford n-butyl 2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate as solid (0.078 g, y. 53%).

¹H NMR (DMSO-d₆) δ (ppm) 10.25 (s, 1H), 7.45-7.55 (m, 2H), 7.21-7.31 (m, 2H), 4.65 (s, 2H), 4.17 (t, J=6.53 Hz, 2H), 1.57-1.67 (m, 2H), 1.33-1.45 (m, 2H), 0.92 (t, J=7.40 Hz, 3H); LCMS (m/z): 293.8 [M+H]⁺.

Second Step

To a solution of n-butyl 2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.035 g, 0.12 mmol) and 7-azaindole-3-carboxaldehyde (0.018 g, 0.12 mmol) in 2-propanol (1.0 mL), L-proline (0.0028 g, 0.024 mmol) was added at ambient temperature. The mixture was refluxed for 2 days. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol and diisopropyl ether then dried to afford the titled compound as solid (0.019 g, y. 36%).

¹H NMR (DMSO-d₆) δ (ppm) 12.28 (br. s, 1H), 10.56 (s, 1H), 8.25 (dd, J=1.38, 4.64 Hz, 1H), 7.95 (d, J=7.78 Hz, 1H), 7.75 (d, J=2.76 Hz, 1H), 7.60-7.68 (m, 2H), 7.35-7.42 (m, 2H), 6.85-6.95 (m, 2H), 4.22 (t, J=6.65 Hz, 2H), 1.60-1.72 (m, 2H), 1.37-1.49 (m, 2H), 0.94 (t, J=7.40 Hz, 3H); LCMS (m/z): 421.9 [M+H]⁺.

Example 53 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(2,4-difluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-[(2,4-difluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.15 g, 0.54 mmol) which similarly prepared according to the procedure described in the Example 2, First step and 7-azaindole-3-carboxaldehyde (0.074 g, 0.50 mmol) in 2-propanol (2.5 mL), 2M hydrochloric acid in ethanol (0.25 mL, 0.50 mmol) was added at ambient temperature then refluxed for 24 h. Cooled with ice bath, aqueous 2M sodium hydroxide solution (0.024 mL, 0.048 mmol) was added dropwise to neutralize and the mixture was refluxed for 1 h. The precipitate was collected by filtration, washed with ethanol and hexane then dried to afford the titled compound as solid (0.14 g, y. 69%).

¹H NMR (DMSO-d₆) δ (ppm) 12.27 (br. s, 1H), 10.51 (s, 1H), 8.24 (dd, J=1.38, 4.64 Hz, 1H), 7.84 (d, J=7.78 Hz, 1H), 7.66-7.77 (m, 2H), 7.50-7.59 (m, 1H), 7.29 (t, J=7.78 Hz, 1H), 6.80-6.89 (m, 2H), 4.26 (q, J=7.03 Hz, 2H), 1.29 (t, J=7.03 Hz, 3H); LCMS (m/z): 411.8 [M+H]⁺.

Example 54 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(3,4-difluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-[(3,4-difluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.084 g, 0.30 mmol) which similarly prepared according to the procedure described in the Example 4, First step and 7-azaindole-3-carboxaldehyde (0.041 g, 0.28 mmol) in 2-propanol (2.0 mL), 2M hydrochloric acid in ethanol (0.14 mL, 0.28 mmol) was added at ambient temperature. The mixture was refluxed for 18 h. Cooled with ice bath, aqueous 2M sodium hydroxide solution (0.13 mL, 0.26 mmol) was added dropwise to neutralize, ethanol (5.0 mL) was added and the mixture was refluxed for 1.5 h. The precipitate was collected by filtration, washed with hot ethanol. The solid was washed with hexane then dried to afford the titled compound as solid (0.051 g, y. 44%).

¹H NMR (DMSO-d₆) δ (ppm) 12.07 (br. s, 1H), 10.63 (br. s, 1H), 8.22 (dd, J=1.38, 4.64 Hz, 1H), 8.00 (d, J=7.78 Hz, 1H), 7.65 (s, 1H), 7.33-7.51 (m, 2H), 7.13 (br. s, 1H), 6.88 (dd, J=4.64, 7.91 Hz, 1H), 6.56 (br. s, 1H), 4.16 (q, J=6.86 Hz, 2H), 1.24 (t, J=7.15 Hz, 3H); LCMS (m/z): 411.8 [M+H]⁺.

Example 55 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-4-oxo-2-{[4-(1-propoxy)phenyl]amino}-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 4-oxo-2-{[4-(1-propoxy)phenyl]amino}-4,5-dihydrofuran-3-carboxylate (0.15 g, 0.50 mmol) which similarly prepared according to the procedure described in the Example 4, First step and 7-azaindole-3-carboxaldehyde (0.072 g, 0.50 mmol) in ethanol (6.0 mL), L-proline (0.0060 g, 0.05 mmol) was added at ambient temperature. The mixture was refluxed for 16 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol then dried to afford the titled compound as solid (0.060 g, y. 28%).

¹H NMR (DMSO-d₆) δ (ppm) 12.30 (br. s, 1H), 10.44 (br. s, 1H), 8.22 (br. s, 1H), 7.85-7.99 (m, 1H), 7.79 (br. s, 1H), 7.48 (d, J=6.85 Hz, 2H), 7.07 (d, J=7.34 Hz, 2H), 6.86 (br. s, 2H), 4.18-4.37 (m, 2H), 3.93-4.11 (m, 2H), 1.70-1.90 (m, 2H), 1.22-1.38 (m, 3H), 0.98-1.12 (m, 3H); LCMS (m/z): 433.9 [M+H]⁺.

Example 56 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(isopropylamino)-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-(isopropylamino)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.50 g, 2.3 mmol) which similarly prepared according to the procedure described in the Example 4, First step and 7-azaindole-3-carboxaldehyde (0.34 g, 2.3 mmol) in ethanol (15 mL), L-proline (0.027 g, 0.23 mmol) was added at ambient temperature. The mixture was refluxed for 16 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol then dried to afford the titled compound as solid (0.45 g, y. 56%).

¹H NMR (DMSO-d₆) δ (ppm) 12.32 (br. s, 1H), 8.56 (br. s, 1H), 8.41 (d, J=7.82 Hz, 1H), 8.32 (d, J=3.91 Hz, 1H), 7.99 (s, 1H), 7.20 (dd, J=4.40, 7.83 Hz, 1H), 6.90 (s, 1H), 4.39 (br. s, 1H), 4.21 (q, J=7.01 Hz, 2H), 1.38 (d, J=6.36 Hz, 6H), 1.26 (t, J=6.85 Hz, 3H); LCMS (m/z): 342.3 [M+H]⁺.

Example 57 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{[2-(dimethylamino)ethyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 56.

¹H NMR (DMSO-d₆) δ (ppm) 12.37 (br. s, 1H), 8.82 (br. s, 1H), 8.42 (d, J=7.82 Hz, 1H), 8.31 (d, J=3.91 Hz, 1H), 8.03 (s, 1H), 7.20 (dd, J=4.89, 7.83 Hz, 1H), 6.90 (s, 1H), 4.20 (q, J=7.34 Hz, 2H), 3.68-3.80 (m, 2H), 2.38-2.65 (m, 2H), 2.23 (s, 6H), 1.26 (t, J=7.09 Hz, 3H); LCMS (m/z): 371.2 [M+H]⁺.

Example 58 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(3-fluorobenzyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 56.

¹H NMR (DMSO-d₆) δ (ppm) 12.33 (br. s, 1H), 9.54 (br. s, 1H), 8.28 (d, J=5.87 Hz, 2H), 7.88 (s, 1H), 7.44 (q, J=7.34 Hz, 1H), 7.20-7.33 (m, 2H), 7.03-7.18 (m, 2H), 6.87 (s, 1H), 4.87 (br. s, 2H), 4.23 (q, J=6.85 Hz, 2H), 1.27 (t, J=6.85 Hz, 3H); LCMS (m/z): 408.0 [M+H]⁺.

Example 59 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(cycloheptylamino)-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 56.

¹H NMR (DMSO-d₆) δ (ppm) 12.37 (br. s, 1H), 8.56 (d, J=8.16 Hz, 1H), 8.42 (d, J=7.82 Hz, 1H), 8.32 (d, J=3.91 Hz, 1H), 7.99 (s, 1H), 7.19 (dd, J=4.89, 7.82 Hz, 1H), 6.90 (s, 1H), 4.21 (q, J=6.85 Hz, 2H), 3.39-3.50 (m, 1H), 1.94-2.08 (m, 2H), 1.77-1.91 (m, 2H), 1.48-1.76 (m, 8H), 1.26 (t, J=7.09 Hz, 3H); LCMS (m/z): 396.0 [M+H]⁺.

Example 60 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-4-oxo-2-[(2-thienylmethyl)amino]-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 56.

¹H NMR (DMSO-d₆) δ (ppm) 12.36 (br. s, 1H), 9.55 (br. s, 1H), 8.37 (d, J=7.82 Hz, 1H), 8.30 (d, J=3.91 Hz, 1H), 8.03 (s, 1H), 7.45 (d, J=4.89 Hz, 1H), 7.09-7.22 (m, 2H), 7.00 (t, J=3.96 Hz, 1H), 6.91 (s, 1H), 5.01 (br. s, 2H), 4.22 (q, J=7.01 Hz, 2H), 1.26 (t, J=6.85 Hz, 3H); LCMS (m/z): 395.8 [M+H]⁺.

Example 61 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(cyclopropylamino)-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 56.

¹H NMR (DMSO-d₆) δ (ppm) 12.33 (br. s, 1H), 8.86 (br. s, 1H), 8.57 (d, J=7.82 Hz, 1H), 8.31 (d, J=3.91 Hz, 1H), 8.06 (s, 1H), 7.21 (dd, J=4.89, 7.82 Hz, 1H), 6.92 (s, 1H), 4.19 (q, J=7.01 Hz, 2H), 3.08-3.20 (m, 1H), 1.24 (t, J=6.85 Hz, 3H), 0.84-1.01 (m, 4H); LCMS (m/z): 340.0 [M+H]⁺.

Example 62 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(3,5-dimethylpiperidino)-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 56.

¹H NMR (DMSO-d₆) δ (ppm) 12.32 (br. s, 1H), 8.38 (d, J=7.82 Hz, 1H), 8.31 (d, J=3.91 Hz, 1H), 7.96 (s, 1H), 7.11-7.26 (m, 1H), 6.84 (s, 1H), 4.00-4.25 (m, 4H), 2.81 (t, J=11.98 Hz, 2H), 1.74-1.93 (m, 3H), 1.24 (t, J=6.85 Hz, 3H), 0.82-1.02 (m, 7H); LCMS (m/z): 396.0 [M+H]⁺.

Example 63 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-azepinyl-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 56.

¹H NMR (DMSO-d₆) δ (ppm) 12.29 (br. s, 1H), 8.38 (d, J=7.78 Hz, 1H), 8.31 (dd, J=1.51, 4.77 Hz, 1H), 7.93 (d, J=2.26 Hz, 1H), 7.19 (dd, J=4.64, 7.91 Hz, 1H), 6.84 (s, 1H), 4.17 (q, J=7.19 Hz, 2H), 3.73-3.84 (m, 4H), 1.75-1.93 (m, 4H), 1.51-1.64 (m, 4H), 1.24 (t, J=7.03 Hz, 3H); LCMS (m/z): 382.0 [M+H]⁺.

Example 64 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(cyclopropylmethyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 56.

¹H NMR (DMSO-d₆) δ (ppm) 12.33 (br. s, 1H), 9.06 (br. s, 1H), 8.41 (d, J=7.82 Hz, 1H), 8.31 (d, J=3.91 Hz, 1H), 8.00 (br. s, 1H), 7.19 (dd, J=4.65, 7.58 Hz, 1H), 6.89 (s, 1H), 4.22 (q, J=7.01 Hz, 2H), 3.52 (t, J=6.36 Hz, 2H), 1.15-1.35 (m, 4H), 0.52 (d, J=6.85 Hz, 2H), 0.36 (d, J=4.40 Hz, 2H); LCMS (m/z): 354.2 [M+H]⁺.

Example 65 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(1-methyl-4-piperidinyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 56.

¹H NMR (DMSO-d₆) δ (ppm) 12.36 (br. s, 1H), 8.58 (br. s, 1H), 8.42 (d, J=7.83 Hz, 1H), 8.33 (d, J=3.42 Hz, 1H), 7.98 (s, 1H), 7.19 (dd, J=4.65, 7.58 Hz, 1H), 6.90 (s, 1H), 4.21 (q, J=6.85 Hz, 2H), 3.90-4.07 (m, 1H), 2.79 (d, J=10.27 Hz, 2H), 2.21 (s, 3H), 2.08 (t, J=10.27 Hz, 2H), 1.80-1.99 (m, 4H), 1.26 (t, J=6.85 Hz, 3H); LCMS (m/z): 397.2 [M+H]⁺.

Example 66 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(4-acetylpiperazinyl)-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 56.

¹H NMR (DMSO-d₆) δ (ppm) 12.37 (br. s, 1H), 8.38 (d, J=7.34 Hz, 1H), 8.32 (d, J=3.42 Hz, 1H), 8.01 (s, 1H), 7.21 (dd, J=4.89, 7.82 Hz, 1H), 6.89 (s, 1H), 4.18 (q, J=7.34 Hz, 2H), 3.77-3.95 (m, 4H), 3.62-3.77 (m, 4H), 2.07 (s, 3H), 1.25 (t, J=6.85 Hz, 3H); LCMS (m/z): 411.4 [M+H]⁺.

Example 67 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(3-pyrazolyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-[(3-pyrazolyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.30 g, 1.3 mmol) which similarly prepared according to the procedure described in the Example 4, First step, and 7-azaindole-3-carboxaldehyde (0.19 g, 1.3 mmol) in ethanol (6.0 mL), L-proline (0.015 g, 0.13 mmol) was added at ambient temperature. The mixture was refluxed for 16 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol then dried to afford the titled compound as solid (0.20 g, y. 35%).

¹H NMR (DMSO-d₆) δ (ppm) 13.07 (br. s, 1H), 12.34 (br. s, 1H), 10.46 (br. s, 1H), 8.27 (d, J=3.91 Hz, 1H), 8.14 (d, J=7.82 Hz, 1H), 7.91-8.20 (m, 2H), 7.08 (dd, J=4.65, 7.58 Hz, 1H), 6.94 (s, 1H), 6.47 (br. s, 1H), 4.26 (q, J=6.85 Hz, 2H), 1.29 (t, J=7.09 Hz, 3H); LCMS (m/z): 366.0 [M+H]⁺.

Example 68 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(6-indazolyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 67.

¹H NMR (DMSO-d₆) δ (ppm) 13.22 (br. s, 1H), 12.24 (br. s, 1H), 10.66 (br. s, 1H), 8.22 (br. s, 1H), 8.12 (br. s, 1H), 7.91 (d, J=8.31 Hz, 1H), 7.71-7.86 (m, 3H), 7.33 (d, J=7.83 Hz, 1H), 6.89 (s, 1H), 6.33 (br. s, 1H), 4.29 (d, J=6.85 Hz, 2H), 1.31 (t, J=6.60 Hz, 3H); LCMS (m/z): 416.4 [M+H]⁺.

Example 69 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[4-(hydroxymethyl)piperidino]-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 67.

¹H NMR (DMSO-d₆) δ (ppm) 12.30 (br. s, 1H), 8.37 (d, J=7.82 Hz, 1H), 8.31 (d, J=3.91 Hz, 1H), 7.97 (br. s, 1H), 7.19 (dd, J=4.40, 7.82 Hz, 1H), 6.84 (s, 1H), 4.57 (t, J=4.89 Hz, 1H), 4.08-4.32 (m, 4H), 3.22-3.40 (m, 4H), 1.70-1.95 (m, 3H), 1.37 (q, J=11.09 Hz, 2H), 1.24 (t, J=6.85 Hz, 3H); LCMS (m/z): 398.0 [M+H]⁺.

Example 70 2-Methoxyethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

First Step

A solution of methyl 2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.063 g, 0.25 mmol) which similarly prepared according to the procedure described in the Example 2, First step using methyl 4-chloroacetoacetate and 4-fluorophenyl isocyanate, 2-methoxyethanol (0.2 mL, 2.5 mmol) and zinc cluster catalyst (Zn₄(OCOCF₃)₆O) (0.0048 g, 0.0050 mmol) in anhydrous dioxane (1.0 mL) was stirred with the microwave synthesizer (Biotage Initiator™) at 120° C. for 1 h. Cooled to ambient temperature, the reaction mixture was concentrated under reduced pressure. The residue was purified by chromatography on silica gel(chloroform/ethyl acetate) to afford 2-methoxyethyl 2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate as solid (0.024 g, y. 33%).

¹H NMR (DMSO-d₆) δ (ppm) 10.22 (s, 1H), 7.43-7.56 (m, 2H), 7.27 (t, J=8.78 Hz, 2H), 4.66 (s, 2H), 4.22-4.37 (m, 2H), 3.52-3.66 (m, 2H), 3.30 (s, 3H); LCMS (m/z): 295.8 [M+H]⁺

Second Step

To a solution of 2-methoxyethyl 2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.023 g, 0.078 mmol) and 7-azaindole-3-carboxaldehyde (0.011 g, 0.078 mmol) in 2-propanol (1.0 mL), L-proline (0.0018 g, 0.016 mmol) was added at ambient temperature. The mixture was refluxed for 2 days. Cooled to ambient temperature, the precipitate was collected by filtration. The solid was purified by preparative HPLC to afford the titled compound as solid (0.046 g, y. 14%).

¹H NMR (DMSO-d₆) δ (ppm) 12.22 (br. s, 1H), 10.65 (br. s, 1H), 8.24 (dd, J=1.25, 4.52 Hz, 1H), 7.96 (d, J=7.53 Hz, 1H), 7.72 (d, J=2.26 Hz, 1H), 7.52-7.63 (m, 2H), 7.35 (t, J=8.78 Hz, 2H), 6.89 (dd, J=4.77, 8.03 Hz, 1H), 6.82 (br. s, 1H), 4.32 (t, J=4.77 Hz, 2H), 3.54-3.72 (m, 2H), 3.33 (s, 3H); LCMS (m/z): 423.8 [M+H]⁺.

Example 71 2-(Dimethylamino)ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate formate

Under a nitrogen atmosphere, a solution of methyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.038 g, 0.10 mmol) which afforded in the Example 51, 2-(dimethylamino)ethanol (0.10 mL, 1.0 mmol) and zinc cluster catalyst (Zn₄(OCOCF₃)₆O) (0.0019 g, 0.0020 mmol) in N,N-dimethylacetamide (0.9 mL) was stirred with the microwave synthesizer (Biotage Initiator™) at 130° C. for 1 h. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC to afford the titled compound as solid (0.016 g, y. 36%).

¹H NMR (DMSO-d₆) δ (ppm) 12.15 (br. s, 1H), 8.23 (dd, J=1.38, 4.64 Hz, 1H), 8.14 (s, 1H), 7.97 (d, J=7.53 Hz, 1H), 7.68 (d, J=2.01 Hz, 1H), 7.45 (dd, J=5.14, 8.41 Hz, 2H), 7.27-7.36 (m, 2H), 6.89 (dd, J=4.64, 7.91 Hz, 1H), 6.72 (s, 1H), 4.30 (t, J=5.77 Hz, 2H), 2.80 (t, J=5.77 Hz, 2H), 2.41 (s, 6H); LCMS (m/z): 436.9 [M+H]⁺.

Example 72 2-Hydroxyethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

Under a nitrogen atmosphere, a solution of the compound (0.038 g, 0.10 mmol) of Example 51, ethylene glycol (0.50 mL, 9.0 mmol) and zinc cluster catalyst (Zn₄(OCOCF₃)₆O) (0.0019 g, 0.0020 mmol) in N,N-dimethylacetamide (0.5 mL) was stirred with the microwave synthesizer (Biotage Initiator™) at 150° C. for 1 h. Cooled to ambient temperature, the precipitate was removed by filtration. The filtrate was purified by preparative HPLC to afford the titled compound as solid (0.0040 g, y. 10%).

¹H NMR (DMSO-d₆) δ (ppm) 12.24 (br. s, 1H), 10.52 (br. s, 1H), 8.21-8.28 (m, 1H), 7.96 (d, J=7.78 Hz, 1H), 7.74 (d, J=2.40 Hz, 1H), 7.58 (br. s, 2H), 7.36 (t, J=8.66 Hz, 2H), 6.80-6.94 (m, 2H), 4.90 (br. s, 1H), 4.22 (t, J=5.27 Hz, 2H), 3.67 (t, J=5.27 Hz, 2H); LCMS (m/z): 409.8 [M+H]⁺.

Example 73 2-Morpholinoethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate formate

Under a nitrogen atmosphere, a solution of the compound (0.038 g, 0.10 mmol) of Example 51, N-(2-hydroxyethyl)morpholine (0.20 mL, 1.6 mmol) and zinc cluster catalyst (Zn₄(OCOCF₃)₆O) (0.0019 g, 0.0020 mmol) in N,N-dimethylacetamide (0.8 mL) was stirred with the microwave synthesizer (Biotage Initiator™) at 150° C. for 40 min. Cooled to ambient temperature, the precipitate was removed by filtration. The filtrate was purified by preparative HPLC to afford the titled compound as solid (0.0075 g, y. 15%).

¹H NMR (DMSO-d₆) δ (ppm) 12.25 (br. s, 1H), 8.24 (d, J=4.02 Hz, 1H), 8.15 (s, 1H), 7.95 (d, J=7.78 Hz, 1H), 7.72 (s, 1H), 7.60 (br. s, 2H), 7.33-7.43 (m, 2H), 6.84-6.93 (m, 2H), 4.31 (t, J=5.77 Hz, 2H), 3.47-3.58 (m, 4H), 2.78 (s, 2H), 2.62-2.70 (m, 2H), 2.35-2.42 (m, 2H); LCMS (m/z): 478.9 [M+H]⁺.

Example 74 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{[4-(2-hydroxyethoxy)-2-methylphenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate

First Step

A solution of 4-nitro-m-cresol (1.4 g, 9.4 mmol), 2-bromoethanol (1.0 mL, 0.014 mol) and potassium carbonate (3.3 g, 0.024 mol) in N,N-dimethylformamide (10 mL) was stirred with heating at 70° C. for 12 h. Cooled to ambient temperature, the reaction mixture was poured into ice water and extracted with ethyl acetate. The organic layer was washed with water and brine, dried over sodium sulfate, filtered and concentrated. The residue was purified by chromatography on silica gel(hexane/ethyl acetate) to afford 2-(3-methyl-4-nitrophenoxy)ethanol as solid (1.3 g, y. 70%).

¹H NMR (DMSO-d₆) δ (ppm) 8.04 (d, J=9.08 Hz, 1H), 7.03 (br. s, 1H), 6.98 (dd, J=2.44, 9.04 Hz, 1H), 4.92 (t, J=5.42 Hz, 1H), 4.11 (t, J=4.80 Hz, 2H), 3.73 (q, J=4.99 Hz, 2H), 2.55 (s, 3H); LCMS (m/z): 198.3 [M+H]⁺.

Second Step

Under a nitrogen atmosphere, 2-(3-methyl-4-nitrophenoxy)ethanol (1.0 g, 5.3 mmol) was dissolved in methanol/tetrahydrofuran (30 mL/30 mL) and 10% palladium on carbon (0.37 g) was added at ambient temperature. The reaction mixture was agitated under a hydrogen atmosphere for 4 h. Palladium on carbon was removed by filtration with Celite and the solvent was removed under reduced pressure to afford 4-(2-hydroxyethoxy)-2-methylaniline as solid (0.80 g, y. 90%).

¹H NMR DMSO-d₆) δ (ppm) 6.57 (br. s, 1H), 6.52 (br. s, 2H), 4.74 (t, J=5.56 Hz, 1H), 4.34 (br. s, 2H), 3.82 (t, J=5.16 Hz, 2H), 3.63 (q, J=5.30 Hz, 2H), 2.02 (s, 3H); LCMS (m/z): 168.0 [M+H]⁺.

Third Step

Diethyl malonate (8.0 mL, 0.052 mol) was added dropwise to a solution of sodium hydride (60% w/w in oil, 4.2 g, 0.11 mol) in anhydrous tetrahydrofuran (130 mL) that cooled with ice bath. The mixture was refluxed for 7 min. The reaction mixture was cooled with ice bath, chloroacetyl chloride (4.2 mL, 0.052 mol) was added dropwise to the reaction mixture and stirred for 1 h then stirred at 45° C. for 1 h. Cooled to ambient temperature, the reaction mixture was diluted with water, and extracted with chloroform for 4 times. The organic layer was dried over magnesium sulfate and concentrated. The residue was purified by chromatography on silica gel (chloroform/methanol) to afford ethyl 2-ethoxy-4-oxo-4,5-dihydrofuran-3-carboxylate as solid (5.0 g, y. 48%).

¹H NMR (CDCl₃) δ (ppm) 4.58-4.70 (m, 4H), 4.30 (q, J=7.03 Hz, 2H), 1.53 (t, J=7.03 Hz, 3H), 1.33 (t, J=7.15 Hz, 3H)

Fourth Step

A solution of 4-(2-hydroxyethoxy)-2-methylaniline (0.69 g, 4.1 mmol) and ethyl 2-ethoxy-4-oxo-4,5-dihydrofuran-3-carboxylate (0.83 g, 4.2 mmol) in ethanol (8.3 mL) was stirred at ambient temperature for 16 h. The solvent was removed under reduced pressure, and ethanol was added to precipitate the product. The precipitate was collected by filtration then dried to afford ethyl 2-{[4-(2-hydroxyethoxy)-2-methylphenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate as solid (0.55 g, y. 42%).

¹H NMR (DMSO-d₆) δ (ppm) 9.99 (br. s, 1H), 7.24 (d, J=7.84 Hz, 1H), 6.89 (br. s, 1H), 6.80 (d, J=8.40 Hz, 1H), 4.83-4.91 (m, 1H), 4.56 (br. s, 2H), 4.20 (q, J=6.95 Hz, 2H), 3.97 (t, J=4.88 Hz, 2H), 3.65-3.74 (m, 2H), 2.20 (s, 3H), 1.24 (t, J=7.04 Hz, 3H); LCMS (m/z): 322.2 [M+H]⁺.

Fifth Step

To a solution of 7-azaindole-3-carboxaldehyde (0.16 g, 1.1 mmol) and ethyl 2-{[4-(2-hydroxyethoxy)-2-methylphenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate (0.36 g, 1.1 mmol) in ethanol (11 mL), L-proline (0.022 g, 0.19 mmol) was added at ambient temperature. The mixture was refluxed for 2 days. Cooled to ambient temperature, the precipitate was collected by filtration, washed with diethyl ether then dried to afford the titled compound as solid (0.24 g, y. 48%).

¹H NMR (DMSO-d₆) δ (ppm) 12.27 (br. s, 1H), 10.29 (br. s, 1H), 8.19 (d, J=3.91 Hz, 1H), 7.75 (br. s, 1H), 7.68 (d, J=7.83 Hz, 1H), 7.41 (d, J=8.31 Hz, 1H), 7.03 (br. s, 1H), 6.94 (d, J=8.31 Hz, 1H), 6.72-6.86 (m, 2H), 4.97 (t, J=5.38 Hz, 1H), 4.26 (q, J=6.85 Hz, 2H), 4.09 (t, J=4.65 Hz, 2H), 3.80 (dd, J=5.14, 9.98 Hz, 2H), 2.23 (s, 3H), 1.29 (t, J=7.09 Hz, 3H); LCMS (m/z): 450.2 [M+H]⁺.

Example 75 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(benzo[d]thiazol-6-ylamino)-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-(benzo[d]thiazol-6-ylamino)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.070 g, 0.23 mmol) which similarly prepared according to the procedure described in the Example 4, First step and 7-azaindole-3-carboxaldehyde (0.034 g, 0.23 mmol) in ethanol (6.0 mL), L-proline (0.0030 g, 0.023 mmol) was added at ambient temperature. The mixture was refluxed for 16 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol then dried to afford the titled compound as solid (0.045 g, y. 45%).

¹H NMR (DMSO-d₆) δ (ppm) 12.25 (br. s, 1H), 10.77 (br. s, 1H), 9.52 (s, 1H), 8.40 (s, 1H), 8.23 (d, J=8.80 Hz, 1H), 8.12 (d, J=3.42 Hz, 1H), 7.69-7.85 (m, 3H), 6.89 (s, 1H), 6.36 (t, J=5.94 Hz, 1H), 4.16-4.47 (m, 2H), 1.31 (t, J=6.85 Hz, 3H); LCMS (m/z): 433.2 [M+H]⁺.

Example 76 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[4-(2-hydroxyethyl)piperazinyl]-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-[4-(2-hydroxyethyl)piperazinyl]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.35 g, 1.2 mmol) which similarly prepared according to the procedure described in the Example 74, Fourth step and 7-azaindole-3-carboxaldehyde (0.18 g, 1.2 mmol) in ethanol (6.0 mL), piperidine (0.12 mL, 1.4 mmol) was added at ambient temperature. The mixture was refluxed for 16 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol then dried to afford the titled compound as solid (0.050 g, y. 10%).

¹H NMR (DMSO-d₆) δ (ppm) 12.34 (br. s, 1H), 8.37 (d, J=7.82 Hz, 1H), 8.31 (d, J=3.91 Hz, 1H), 7.99 (s, 1H), 7.20 (dd, J=4.89, 7.82 Hz, 1H), 6.87 (s, 1H), 4.50 (br. s, 1H), 4.16 (q, J=7.34 Hz, 2H), 3.71-3.88 (m, 4H), 3.55 (t, J=5.87 Hz, 2H), 2.57-2.72 (m, 4H), 2.40-2.58 (m, 2H), 1.24 (t, J=7.09 Hz, 3H); LCMS (m/z): 413.2 [M+H]⁺.

Example 77 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(4-hydroxypiperidino)-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 76.

¹H NMR (DMSO-d₆) δ (ppm) 12.30 (br. s, 1H), 8.37 (d, J=7.82 Hz, 1H), 8.31 (d, J=3.42 Hz, 1H), 7.98 (s, 1H), 7.19 (dd, J=4.40, 7.83 Hz, 1H), 6.85 (s, 1H), 4.92 (d, J=3.91 Hz, 1H), 4.17 (q, J=6.85 Hz, 2H), 3.92-4.02 (m, 2H), 3.80-3.90 (m, 1H), 3.58 (t, J=9.29 Hz, 2H), 1.94 (d, J=3.42 Hz, 2H), 1.52-1.69 (m, 2H), 1.24 (t, J=6.85 Hz, 3H); LCMS (m/z): 384.2 [M+H]⁺.

Example 78 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(4-methylpiperidino)-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 76.

¹H NMR (DMSO-d₆) δ (ppm) 12.30 (br. s, 1H), 8.37 (d, J=7.34 Hz, 1H), 8.31 (d, J=3.91 Hz, 1H), 7.97 (s, 1H), 7.19 (dd, J=4.40, 7.82 Hz, 1H), 6.84 (s, 1H), 4.08-4.29 (m, 4H), 3.22-3.40 (m, 2H), 1.68-1.92 (m, 3H), 1.28-1.42 (m, 2H), 1.24 (t, J=6.85 Hz, 3H), 0.96 (d, J=5.87 Hz, 3H); LCMS (m/z): 382.2 [M+H]⁺.

Example 79 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-morpholino-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 76.

¹H NMR (DMSO-d₆) δ (ppm) 12.34 (br. s, 1H), 8.37 (d, J=7.82 Hz, 1H), 8.31 (d, J=3.42 Hz, 1H), 8.00 (s, 1H), 7.20 (dd, J=4.40, 7.82 Hz, 1H), 6.89 (s, 1H), 4.16 (q, J=7.17 Hz, 2H), 3.73-3.89 (m, 8H), 1.24 (t, J=7.09 Hz, 3H); LCMS (m/z): 370.4 [M+H]⁺.

Example 80 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{[2-fluoro-4-(2-methoxyethoxy)phenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-{[2-fluoro-4-(2-methoxyethoxy)phenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate (0.011 g, 0.032 mmol) which similarly prepared according to the procedure described in the Example 29, First step and 7-azaindole-3-carboxaldehyde (0.0045 g, 0.031 mmol) in ethanol (0.2 mL), 2M hydrochloric acid in ethanol (0.016 mL, 0.032 mmol) was added at ambient temperature. The mixture was refluxed for 4 h. Cooled with ice bath, 2M sodium hydroxide solution (0.015 mL, 0.029 mmol) was added dropwise to neutralize, and ethanol (1.5 mL) was added. The mixture was refluxed for further 30 min. The precipitate was collected by filtration, washed with hot ethanol. The solid was washed with hexane then dried to afford the titled compound as solid (0.0051 g, y. 32%).

¹H NMR (DMSO-d₆) δ (ppm) 12.34 (br. s, 1H), 10.38 (s, 1H), 8.21 (dd, J=1.38, 4.64 Hz, 1H), 7.81 (d, J=7.53 Hz, 1H), 7.75 (d, J=2.51 Hz, 1H), 7.58 (t, J=9.03 Hz, 1H), 7.13 (dd, J=2.51, 12.05 Hz, 1H), 6.98 (dd, J=2.26, 8.78 Hz, 1H), 6.89 (s, 1H), 6.82 (dd, J=4.64, 7.91 Hz, 1H), 4.20-4.31 (m, 4H), 3.71-3.77 (m, 2H), 3.37 (br. s, 3H), 1.29 (t, J=7.03 Hz, 3H); LCMS (m/z): 467.9 [M+H]⁺.

Example 81 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{[2-fluoro-4-(2-hydroxyethoxy)phenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-{[2-fluoro-4-(2-hydroxyethoxy)phenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate (0.017 g, 0.052 mmol) which similarly prepared according to the procedure described in the Example 29, First step and 7-azaindole-3-carboxaldehyde (0.0075 g, 0.051 mmol) in ethanol (0.2 mL), 2M hydrochloric acid in ethanol (0.052 mL, 0.10 mmol) was added at ambient temperature. The mixture was refluxed for 8.5 h. Cooled with ice bath, 2M sodium hydroxide solution (0.047 mL, 0.094 mmol) was added dropwise to neutralize, and ethanol (0.5 mL) was added and refluxed for further 16 min. The precipitate was collected by filtration, washed with hot ethanol. The solid was washed with hexane then dried to afford the titled compound as solid (0.014 g, y. 59%).

¹H NMR (DMSO-d₆) δ (ppm) 12.14 (br. s, 1H), 10.38 (s, 1H), 8.21 (dd, J=1.20, 4.80 Hz, 1H), 7.87 (d, J=7.20 Hz, 1H), 7.65 (br. s, 1H), 7.38 (br. s, 1H), 6.95-7.07 (m, 1H), 6.85-6.95 (m, 1H), 6.83 (dd, J=4.80, 8.00 Hz, 1H), 6.64 (br. s, 1H), 4.96 (t, J=5.40 Hz, 1H), 4.15-4.25 (m, 2H), 4.09 (t, J=4.80 Hz, 2H), 3.78 (q, J=5.07 Hz, 2H), 1.26 (t, J=7.00 Hz, 3H); LCMS (m/z): 453.8 [M+H]⁺.

Example 82 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{[2-(2-methoxyethoxy)-4-methylphenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-{[2-(2-methoxyethoxy)-4-methylphenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate (0.015 g, 0.045 mmol) which similarly prepared according to the procedure described in the Example 29, First step and 7-azaindole-3-carboxaldehyde (0.0067 g, 0.046 mmol) in ethanol (0.2 mL), 2M hydrochloric acid in ethanol (0.023 mL, 0.045 mmol) was added at ambient temperature. The mixture was refluxed for 4 h. Cooled to ambient temperature, the solvent was removed under reduced pressure. The residue was purified by preparative HPLC to afford the titled compound as solid (0.0090 g, y. 43%).

¹H NMR (DMSO-d₆) δ (ppm) 12.29 (br. s, 1H), 10.30 (br. s, 1H), 8.25 (dd, J=1.38, 4.64 Hz, 1H), 8.00 (d, J=7.03 Hz, 1H), 7.83 (s, 1H), 7.49 (d, J=7.78 Hz, 1H), 7.10 (s, 1H), 6.84-6.95 (m, 3H), 4.26 (q, J=7.03 Hz, 2H), 4.09-4.17 (m, 2H), 3.53-3.60 (m, 2H), 3.17 (s, 3H), 2.43 (s, 3H), 1.29 (t, J=7.03 Hz, 3H); LCMS (m/z): 463.9 [M+H]⁺.

Example 83 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-({2-[2-(dimethylamino)ethoxy]-4-methylphenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate

To a stirred solution of ethyl 2-({2-[2-(dimethylamino)ethoxy]-4-methylphenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.011 g, 0.030 mmol) which similarly prepared according to the procedure described in the Example 29, First step and 7-azaindole-3-carboxaldehyde (0.0044 g, 0.030 mmol) in ethanol (0.2 mL), 2M hydrochloric acid in ethanol (0.045 mL, 0.091 mmol) was added at ambient temperature. The mixture was refluxed for 9 h. Cooled with ice bath, 2M sodium hydroxide solution (0.041 mL, 0.082 mmol) was added dropwise to neutralize, and ethanol (0.5 mL) was added. The mixture was refluxed for further 16 min. The precipitate was collected by filtration, washed with hot ethanol. The solid was washed with hexane then dried to afford the titled compound as solid (0.00079 g, y. 5%).

¹H NMR (DMSO-d₆) δ (ppm) 12.27 (br. s, 1H), 8.24 (d, J=4.52 Hz, 1H), 8.16 (s, 1H), 7.96 (d, J=8.03 Hz, 1H), 7.79-7.82 (m, 1H), 7.46 (d, J=7.53 Hz, 1H), 7.09-7.12 (m, 1H), 6.93 (d, J=7.53 Hz, 1H), 6.81-6.89 (m, 2H), 4.25 (q, J=6.94 Hz, 2H), 4.10 (t, J=4.64 Hz, 2H), 2.52-2.58 (m, 2H), 2.43 (s, 3H), 2.13 (s, 6H), 1.29 (t, J=7.03 Hz, 3H); LCMS (m/z): 477.0 [M+H]⁺.

Example 84 Isopropyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

Under a nitrogen atmosphere, a solution of the compound (0.039 g, 0.10 mmol) of Example 21, 4-dimethylaniline (0.0024 g, 0.020 mmol) and zinc cluster catalyst (Zn₄(OCOCF₃)₆O) (0.0012 g, 0.0013 mmol) in 2-propanol (1.0 mL) and N,N-dimethylacetamide (1.0 mL) was stirred with the microwave synthesizer (Biotage Initiator™) at 150° C. for 1.5 h. Cooled to ambient temperature, the precipitate was removed by filtration. The filtrate was purified by preparative HPLC to afford the titled compound as solid (0.011 g, y. 27%).

¹H NMR (DMSO-d₆) δ (ppm) 12.29 (br. s, 1H), 10.54 (s, 1H), 8.25 (dd, J=1.51, 4.77 Hz, 1H), 7.94 (d, J=7.03 Hz, 1H), 7.76 (d, J=2.76 Hz, 1H), 7.56-7.70 (m, 2H), 7.28-7.44 (m, 2H), 6.80-6.98 (m, 2H), 5.08-5.17 (m, 1H), 1.31 (d, J=6.27 Hz, 6H); LCMS (m/z): 408.0 [M+H]⁺.

Example 85 Cyclopropylmethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

Under a nitrogen atmosphere, a solution of the compound (0.038 g, 0.10 mmol) of Example 51, cyclopropyl carbinol (0.10 mL, 1.3 mmol) and zinc cluster catalyst (Zn₄(OCOCF₃)₆O) (0.0019 g, 0.0020 mmol) in N,N-dimethylacetamide (0.9 mL) was stirred with the microwave synthesizer (Biotage Initiator™) at 150° C. for 30 min. Cooled to ambient temperature, the precipitate was removed by filtration. The filtrate was purified by preparative HPLC to afford the titled compound as solid (0.0080 g, y. 19%).

¹H NMR (DMSO-d₆) δ (ppm) 12.29 (br. s, 1H), 10.57 (s, 1H), 8.25 (d, J=3.51 Hz, 1H), 7.95 (d, J=7.78 Hz, 1H), 7.75 (d, J=2.51 Hz, 1H), 7.60-7.69 (m, 2H), 7.34-7.43 (m, 2H), 6.85-6.95 (m, 2H), 4.08 (d, J=7.03 Hz, 2H), 1.16-1.31 (m, 1H), 0.55 (dd, J=1.51, 8.03 Hz, 2H), 0.36 (d, J=5.02 Hz, 2H); LCMS (m/z): 419.8 [M+H]⁺.

Example 86 Methyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(2,4-difluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of methyl 2-[(2,4-difluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.27 g, 1.0 mmol) which similarly prepared according to the procedure described in the Example 2, First step using methyl 4-chloroacetoacetate and 2,4-difluorophenyl isocyanate, and 7-azaindole-3-carboxaldehyde (0.15 g, 1.0 mmol) in 2-propanol (5.0 mL), L-proline (0.023 g, 0.20 mmol) was added at ambient temperature. The mixture was refluxed for 12 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol and diisopropyl ether then dried to afford the titled compound as solid (0.30 g, y. 75%).

¹H NMR (DMSO-d₆) δ (ppm) 12.32 (br. s, 1H), 10.53 (s, 1H), 8.24 (dd, J=1.38, 4.64 Hz, 1H), 7.74-7.87 (m, 2H), 7.70 (d, J=2.51 Hz, 1H), 7.54-7.63 (m, 1H), 7.27-7.37 (m, 1H), 6.94 (s, 1H), 6.84 (dd, J=4.77, 8.03 Hz, 1H), 3.77 (s, 3H); LCMS (m/z): 397.9 [M+H]⁺.

Example 87 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[bis(2-methoxyethyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-[bis(2-methoxyethyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.30 g, 1.0 mmol) which similarly prepared according to the procedure described in the Example 4, First step and 7-azaindole-3-carboxaldehyde (0.15 g, 1.0 mmol) in ethanol (5.0 mL), L-proline (0.023 g, 0.20 mmol) was added at ambient temperature. The mixture was refluxed for 2 days. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol then dried to afford the titled compound as solid (0.048 g, y. 11%).

¹H NMR (DMSO-d₆) δ (ppm) 12.36 (br. s, 1H), 8.38 (d, J=7.82 Hz, 1H), 8.31 (d, J=4.89 Hz, 1H), 7.98 (s, 1H), 7.19 (dd, J=4.65, 8.07 Hz, 1H), 6.85 (s, 1H), 4.16 (q, J=6.85 Hz, 2H), 3.97 (t, J=5.14 Hz, 4H), 3.57-3.67 (m, 4H), 3.25 (s, 6H), 1.24 (t, J=7.09 Hz, 3H); LCMS (m/z): 416.2 [M+H]⁺.

Example 88 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{[4-(hydroxymethyl)phenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 87.

¹H NMR (DMSO-d₆) δ (ppm) 12.27 (br. s, 1H), 10.66 (br. s, 1H), 8.22 (d, J=3.42 Hz, 1H), 7.97 (d, J=7.34 Hz, 1H), 7.79 (br. s, 1H), 7.42-7.55 (m, 4H), 6.86-6.94 (m, 1H), 6.84 (br. s, 1H), 5.34 (br. s, 1H), 4.62 (d, J=4.40 Hz, 2H), 4.26 (d, J=6.85 Hz, 2H), 1.29 (t, J=6.85 Hz, 3H); LCMS (m/z): 406.4 [M+H]⁺.

Example 89 3-Hydroxypropyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

A solution of the compound of Example 51 and zinc cluster catalyst (Zn₄(OCOCF₃)₆O) (0.0019 g, 0.0020 mmol) in 1,3-propanediol (0.5 mL) and N,N-dimethylacetamide (0.5 mL) was stirred at 120° C. for 2 h then at 100° C. for 12 h. Cooled to ambient temperature, the reaction mixture was diluted with water, and the precipitate was collected by filtration. Ethyl acetate was added to the filtrate, and the precipitate was collected by filtration. Those precipitates were combined then purified by preparative HPLC to afford the titled compound as solid (0.0067 g, y. 16%).

¹H NMR (DMSO-d₆) δ (ppm) 12.22 (br. s, 1H), 10.72 (br. s, 1H), 8.24 (dd, J=1.38, 4.64 Hz, 1H), 7.97 (d, J=7.03 Hz, 1H), 7.72 (d, J=2.51 Hz, 1H), 7.50-7.60 (m, 2H), 7.35 (t, J=8.78 Hz, 2H), 6.89 (dd, J=4.52, 8.03 Hz, 1H), 6.81 (br. s, 1H), 4.77 (br. s, 1H), 4.25 (t, J=6.27 Hz, 2H), 3.59 (t, J=5.77 Hz, 2H), 1.79-1.87 (m, 2H); LCMS (m/z): 423.8 [M+H]⁺.

Example 90 Isopropyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(2,4-difluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

A solution of the compound (0.040 g, 0.10 mmol) of Example 86 and zinc cluster catalyst (Zn₄(OCOCF₃)₆O) (0.0012 g, 0.0013 mmol) in 2-propanol (0.5 mL) was stirred at 95° C. for 4 days. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC to afford the titled compound as solid (0.0061 g, y. 13%).

¹H NMR (DMSO-d₆) δ (ppm) 12.32 (br. s, 1H), 10.47 (s, 1H), 8.24 (dd, J=1.51, 4.52 Hz, 1H), 7.67-7.88 (m, 3H), 7.54-7.63 (m, 1H), 7.26-7.38 (m, 1H), 6.88 (s, 1H), 6.83 (dd, J=4.64, 7.91 Hz, 1H), 5.10-5.18 (m, 1H), 1.31 (d, J=6.27 Hz, 6H); LCMS (m/z): 426.2 [M+H]⁺.

Example 91 2-(Dimethylamino)ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(2,4-difluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

A solution of the compound (0.040 g, 0.10 mmol) of Example 86, 2-dimethylaminoethanol (0.10 mL, 1.0 mmol) and zinc cluster catalyst (Zn₄(OCOCF₃)₆O) (0.0019 g, 0.0020 mmol) in N,N-dimethylacetamide (0.9 mL) was stirred at 100° C. for 24 h. Cooled to ambient temperature, the precipitate was removed by filtration. The filtrate was purified by preparative HPLC to afford the titled compound as solid (0.010 g, y. 22%).

¹H NMR (DMSO-d₆) δ (ppm) 11.97 (br. s, 1H), 8.19 (dd, J=1.51, 4.52 Hz, 1H), 8.14 (s, 1H), 7.93 (d, J=7.78 Hz, 1H), 7.52 (br. s, 1H), 7.16-7.36 (m, 2H), 7.03-7.13 (m, 1H), 6.83 (dd, J=4.89, 7.91 Hz, 1H), 6.42 (s, 1H), 4.35 (t, J=5.27 Hz, 2H), 3.22 (t, J=5.27 Hz, 2H), 2.67-2.87 (m, 6H); LCMS (m/z): 455.2 [M+H]⁺.

Example 92 5-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(2,4-difluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylic acid

To a solution of the compound (0.20 g, 0.45 mmol) of Example 53 in ethanol (2.0 mL), aqueous 50% w/v potassium hydroxide solution (0.5 mL) was added at ambient temperature. The mixture was stirred at 95° C. for 3 h. Cooled to ambient temperature, diluted with water and conc. hydrochloric acid was added dropwise to neutralize. The precipitate was collected by filtration, washed with ethanol and diisopropyl ether then dried to afford the titled compound as solid (0.19 g, y. 98%).

¹H NMR (DMSO-d₆) δ (ppm) 12.36 (br. s, 1H), 8.25 (dd, J=1.51, 4.52 Hz, 1H), 7.86 (d, J=7.03 Hz, 1H), 7.69-7.82 (m, 2H), 7.57 (ddd, J=2.76, 8.97, 10.35 Hz, 1H), 7.25-7.37 (m, 1H), 7.01 (s, 1H), 6.85 (dd, J=4.77, 8.03 Hz, 1H); LCMS (m/z): 381.9 [M−H]⁻

Example 93 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{[2-(2-hydroxyethoxy)-4-methylphenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(2,4-difluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.017 g, 0.052 mmol) which similarly prepared according to the procedure described in the Example 29, First step and 7-azaindole-3-carboxaldehyde (0.0075 g, 0.051 mmol) in ethanol (0.2 mL), 2M hydrochloric acid in ethanol (0.052 mL, 0.10 mmol) was added at ambient temperature. The mixture was refluxed for 8.5 h. Cooled with ice bath, 2M sodium hydroxide solution (0.047 mL, 0.094 mmol) was added dropwise to neutralize then the solvent was removed under reduced pressure. The residue was purified by preparative HPLC to afford the titled compound as solid (0.0066 g, y. 28%).

¹H NMR (DMSO-d₆) δ (ppm) 12.30 (br. s, 1H), 10.22 (br. s, 1H), 8.25 (d, J=3.51 Hz, 1H), 8.03 (d, J=7.78 Hz, 1H), 7.83 (br. s, 1H), 7.43-7.56 (m, 1H), 7.10 (s, 1H), 6.85-6.95 (m, 3H), 4.79 (br. s, 1H), 4.26 (q, J=7.19 Hz, 2H), 4.06 (t, J=5.14 Hz, 2H), 3.62 (t, J=5.20 Hz, 2H), 2.42 (s, 3H), 1.29 (t, J=7.15 Hz, 3H); LCMS (m/z): 449.5 [M+H]⁺.

Example 94 Methyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(cyclopropylamino)-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of methyl 2-(cyclopropylamino)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.50 g, 2.5 mmol) which similarly prepared according to the procedure described in the Example 4, First step using dimethyl malonate, chloroacetyl chloride and cyclopropylamine, and 7-azaindole-3-carboxaldehyde (0.37 g, 2.5 mmol) in ethanol (6.0 mL), L-proline (0.029 g, 0.25 mmol) was added at ambient temperature. The mixture was refluxed for 16 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with methanol then dried to afford the titled compound as solid (0.012 g, y. 30%).

¹H NMR (DMSO-d₆) δ (ppm) 12.33 (br. s, 1H), 8.88 (br. s, 1H), 8.57 (d, J=7.82 Hz, 1H), 8.31 (d, J=3.91 Hz, 1H), 8.06 (s, 1H), 7.20 (dd, J=4.89, 7.82 Hz, 1H), 6.94 (s, 1H), 3.69 (s, 3H), 3.08-3.20 (m, 1H), 0.84-1.00 (m, 4H); LCMS (m/z): 326.0 [M+H]⁺.

Example 95 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(2-hydroxy-4-methylphenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-[(2-hydroxy-4-methylphenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.10 g, 0.36 mmol) which similarly prepared according to the procedure described in the Example 74, Fourth step and 7-azaindole-3-carboxaldehyde (0.061 g, 0.42 mmol) in ethanol (2.5 mL), 2M hydrochloric acid in ethanol (0.56 mL, 1.1 mmol) was added at ambient temperature. The mixture was refluxed for 19 h. Cooled with ice bath, 2M sodium hydroxide solution (0.49 mL, 0.97 mmol) was added dropwise to neutralize, and ethanol (8.0 mL) was added and the mixture was refluxed for 30 min. The precipitate was collected by filtration, washed with hot ethanol. The solid was washed with hexane then dried to afford the titled compound as solid (0.027 g, y. 18%).

¹H NMR (DMSO-d₆) δ (ppm) 12.32 (br. s, 1H), 10.21 (s, 1H), 10.19 (s, 1H), 8.22-8.30 (m, 1H), 8.04 (d, J=7.53 Hz, 1H), 7.87 (d, J=2.26 Hz, 1H), 7.44 (d, J=8.03 Hz, 1H), 6.84-6.94 (m, 3H), 6.77 (d, J=8.03 Hz, 1H), 4.27 (q, J=7.03 Hz, 2H), 2.35 (s, 3H), 1.30 (t, J=7.03 Hz, 3H); LCMS (m/z): 405.9 [M+H]⁺.

Example 96 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-hydroxy-2-methylphenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-[(4-hydroxy-2-methylphenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.10 g, 0.37 mmol) which similarly prepared according to the procedure described in the Example 74, Fourth step and 7-azaindole-3-carboxaldehyde (0.063 g, 0.43 mmol) in ethanol (2.5 mL), 2M hydrochloric acid in ethanol (0.46 mL, 0.92 mmol) was added at ambient temperature. The mixture was refluxed for 2.5 days. Cooled with ice bath, 2M sodium hydroxide solution (0.46 mL, 0.92 mmol) was added dropwise to neutralize, and ethanol (11 mL) was added and the mixture was refluxed for further 40 min. The precipitate was collected by filtration, washed with hot ethanol. The solid was washed with hexane then dried to afford the titled compound as solid (0.10 g, y. 70%).

¹H NMR (DMSO-d₆) δ (ppm) 12.27 (br. s, 1H), 10.20 (s, 1H), 9.76 (s, 1H), 8.20 (dd, J=1.25, 4.52 Hz, 1H), 7.81 (d, J=2.51 Hz, 1H), 7.64 (d, J=7.53 Hz, 1H), 7.28 (d, J=8.28 Hz, 1H), 6.72-6.87 (m, 4H), 4.26 (q, J=7.03 Hz, 2H), 2.16 (s, 3H), 1.29 (t, J=7.03 Hz, 3H); LCMS (m/z): 405.8 [M+H]⁺.

Example 97 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-fluoro-2-methylphenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-[(4-fluoro-2-methylphenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.30 g, 1.1 mmol) which similarly prepared according to the procedure described in the Example 4, First step and 7-azaindole-3-carboxaldehyde (0.16 g, 1.1 mmol) in ethanol (7.0 mL), L-proline (0.013 g, 0.10 mmol) was added at ambient temperature. The mixture was refluxed for 3 days. Cooled to ambient temperature, the precipitate was collected by filtration, washed with methanol then dried to afford the titled compound as solid (0.25 g, y. 58%).

¹H NMR (DMSO-d₆) δ (ppm) 12.28 (br. s, 1H), 10.40 (s, 1H), 8.22 (d, J=3.91 Hz, 1H), 7.66-7.76 (m, 2H), 7.59 (dd, J=5.62, 8.56 Hz, 1H), 7.36 (dd, J=2.45, 9.78 Hz, 1H), 7.18-7.28 (m, 1H), 6.85 (s, 1H), 6.78 (dd, J=4.89, 7.83 Hz, 1H), 4.27 (q, J=7.01 Hz, 2H), 2.27 (s, 3H), 1.30 (t, J=7.09 Hz, 3H); LCMS (m/z): 408.4 [M+H]⁺.

Example 98 Methyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(2-methoxyethyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 97.

¹H NMR (DMSO-d₆) δ (ppm) 12.36 (br. s, 1H), 8.94 (br. s, 1H), 8.44 (d, J=7.83 Hz, 1H), 8.31 (d, J=4.40 Hz, 1H), 8.03 (s, 1H), 7.19 (dd, J=4.40, 7.82 Hz, 1H), 6.92 (s, 1H), 3.76-3.84 (m, 2H), 3.71 (s, 3H), 3.62 (t, J=5.14 Hz, 2H), 3.31 (br. s, 3H); LCMS (m/z): 344.2 [M+H]⁺.

Example 99 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{[4-(2-methoxyethoxy)-2-methylphenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate

First Step

To a solution of ethyl 2-[(4-hydroxy-2-methylphenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.10 g, 0.36 mmol) which similarly prepared according to the procedure described in the Example 74, Fourth step, 2-methoxyethanol (0.051 mL, 0.65 mmol) and triphenylphosphine (0.19 g, 0.72 mmol) in dichloromethane (2.0 mL) that cooled with ice bath, 2.2M diethyl azodicarboxylate (40% in toluene, 0.33 mL, 0.73 mmol) was added dropwise then the mixture was stirred at ambient temperature for 24 h. The reaction mixture was diluted with water, and extracted with chloroform. The organic layer was washed with 1M sodium hydroxide solution and brine, dried over magnesium sulfate and concentrated to afford ethyl 2-{[4-(2-methoxyethoxy)-2-methylphenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate as oil (0.30 g, crude material).

Second Step

To a solution of ethyl 2-{[4-(2-methoxyethoxy)-2-methylphenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate (0.12 g, 0.36 mmol, crude material) which afforded in the previous step and 7-azaindole-3-carboxaldehyde (0.043 g, 0.30 mmol) in ethanol (4.0 mL), 2M hydrochloric acid in ethanol (0.30 mL, 0.60 mmol) was added at ambient temperature. The mixture was refluxed for 24 h. Cooled with ice bath, 2M sodium hydroxide solution (0.30 mL, 0.60 mmol) was added dropwise to neutralize, and the solvent was removed under reduced pressure. The residue was suspended in chloroform, then the precipitate was collected by filtration, washed with water, ethanol and hexane then dried to afford the titled compound as solid (0.028 g, y. 12%).

¹H NMR (DMSO-d₆) δ (ppm) 11.78 (br. s, 1H), 8.13 (d, J=3.51 Hz, 1H), 7.89 (d, J=7.53 Hz, 1H), 7.47 (s, 1H), 6.77-6.90 (m, 3H), 6.72 (d, J=8.28 Hz, 1H), 6.12 (s, 1H), 4.05-4.14 (m, 2H), 3.66-3.74 (m, 2H), 3.36 (s, 3H), 3.28-3.35 (m, 2H), 2.08 (s, 3H), 1.22 (t, J=7.03 Hz, 3H); LCMS (m/z): 464.0 [M+H]⁺.

Example 100 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{[2-methyl-4-(2-morpholinoethoxy)phenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate

To a stirred solution of ethyl 2-({2-methyl-4-[2-(N-morpholino)ethoxy]phenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.14 g, 0.36 mmol) which similarly prepared according to the procedure described in the Example 99, First step and 7-azaindole-3-carboxaldehyde (0.038 g, 0.26 mmol) in ethanol (4.0 mL), 2M hydrochloric acid in ethanol (0.60 mL, 1.2 mmol) was added at ambient temperature. The mixture was refluxed for 2 days. Cooled with ice bath, 2M sodium hydroxide solution (0.57 mL, 1.1 mmol) was added dropwise to neutralize and refluxed for further 10 min. Then the solvent was removed under reduced pressure. The precipitate was collected by filtration, washed with water and ethyl acetate then dried to afford the titled compound as solid (0.010 g, y. 5%).

¹H NMR (DMSO-d₆) δ (ppm) 12.28 (br. s, 1H), 10.29 (s, 1H), 8.19 (dd, J=1.26, 4.52 Hz, 1H), 7.77 (s, 1H), 7.67 (d, J=7.78 Hz, 1H), 7.42 (d, J=8.53 Hz, 1H), 7.05 (br. s, 1H), 6.95 (d, J=8.28 Hz, 1H), 6.83 (s, 1H), 6.74 (dd, J=4.77, 7.78 Hz, 1H), 4.27 (q, J=7.03 Hz, 2H), 4.15-4.23 (m, 2H), 3.58-3.68 (m, 4H), 2.74-2.82 (m, 2H), 2.50-2.60 (m, 4H), 2.23 (s, 3H), 1.30 (t, J=7.03 Hz, 3H); LCMS (m/z): 519.0 [M+H]⁺.

Example 101 Methyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(2-fluorobenzyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of methyl 2-[(2-fluorobenzyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.35 g, 1.5 mmol) which similarly prepared according to the procedure described in the Example 4, First step using dimethyl malonate, chloroacetyl chloride and 2-fluorobenzylamine, and 7-azaindole-3-carboxaldehyde (0.22 g, 1.5 mmol) in ethanol (10 mL), L-proline (0.018 g, 0.15 mmol) was added at ambient temperature. The mixture was refluxed for 3 days. Cooled to ambient temperature, the precipitate was collected by filtration, washed with methanol then dried to afford the titled compound as solid (0.13 g, y. 24%).

¹H NMR (DMSO-d₆) δ (ppm) 12.36 (br. s, 1H), 9.49 (br. s, 1H), 8.28 (br. s, 2H), 7.86 (br. s, 1H), 7.16-7.53 (m, 4H), 7.07 (br. s, 1H), 6.90 (br. s, 1H), 4.91 (br. s, 2H), 3.73 (br. s, 3H); LCMS (m/z): 394.4 [M+H]⁺.

Example 102 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(3,4-dihydroisoquinolin-2(1H)-yl)-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-(3,4-dihydroisoquinolin-2(1H-)yl)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.15 g, 0.60 mmol) which similarly prepared according to the procedure described in the Example 4, First step and 7-azaindole-3-carboxaldehyde (0.091 g, 0.60 mmol) in ethanol (5.0 mL), L-proline (0.0070 g, 0.060 mmol) was added at ambient temperature. The mixture was refluxed for 3 days. Cooled to ambient temperature, the precipitate was collected by filtration, washed with methanol then dried to afford the titled compound as solid (0.037 g, y. 14%).

¹H NMR (DMSO-d₆) δ (ppm) 12.36 (br. s, 1H), 8.42 (d, J=7.82 Hz, 1H), 8.33 (d, J=3.91 Hz, 1H), 8.07 (s, 1H), 7.17-7.37 (m, 5H), 6.90 (s, 1H), 4.98 (br. s, 2H), 4.21 (q, J=6.85 Hz, 2H), 3.97 (br. s, 2H), 3.09 (t, J=5.62 Hz, 2H), 1.27 (t, J=7.09 Hz, 3H); LCMS (m/z): 416.2 [M+H]⁺.

Example 103 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(cyclopentylamino)-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 102.

¹H NMR (DMSO-d₆) δ (ppm) 12.32 (br. s, 1H), 8.53 (br. s, 1H), 8.42 (d, J=7.82 Hz, 1H), 8.32 (d, J=4.40 Hz, 1H), 8.01 (s, 1H), 7.20 (dd, J=4.40, 7.83 Hz, 1H), 6.90 (s, 1H), 4.50 (br. s, 1H), 4.21 (q, J=6.85 Hz, 2H), 2.02-2.15 (m, 2H), 1.57-1.71 (m, 6H), 1.26 (t, J=7.09 Hz, 3H); LCMS (m/z): 368.0 [M+H]⁺.

Example 104 Methyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(cyclopropylmethyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of methyl 2-[(cyclopropylmethyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.30 g, 1.4 mmol) which similarly prepared according to the procedure described in the Example 4, First step using dimethyl malonate, chloroacetyl chloride and cyclopropylmethylamine, and 7-azaindole-3-carboxaldehyde (0.21 g, 1.4 mmol) in ethanol (10 mL), L-proline (0.016 g, 0.14 mmol) was added at ambient temperature. The mixture was refluxed for 3 days. Cooled to ambient temperature, the precipitate was collected by filtration, washed with methanol then dried to afford the titled compound as solid (0.065 g, y. 14%).

¹H NMR (DMSO-d₆) δ (ppm) 12.33 (br. s, 1H), 9.08 (br. s, 1H), 8.42 (d, J=7.82 Hz, 1H), 8.31 (d, J=3.42 Hz, 1H), 8.00 (s, 1H), 7.19 (dd, J=4.40, 7.34 Hz, 1H), 6.92 (s, 1H), 3.72 (s, 3H), 3.52 (d, J=6.36 Hz, 2H), 1.23 (br. s, 1H), 0.52 (d, J=7.34 Hz, 2H), 0.37 (d, J=3.91 Hz, 2H); LCMS (m/z): 340.4 [M+H]⁺.

Example 105 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-isoindolinyl-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-isoindolinyl-4-oxo-4,5-dihydrofuran-3-carboxylate (0.20 g, 0.70 mmol) which similarly prepared according to the procedure described in the Example 4, First step and 7-azaindole-3-carboxaldehyde (0.11 g, 0.70 mmol) in ethanol (10 mL), L-proline (0.0080 g, 0.070 mmol) was added at ambient temperature. The mixture was refluxed for 3 days. Cooled to ambient temperature, the precipitate was collected by filtration, washed with methanol then dried to afford the titled compound as solid (0.080 g, y. 27%).

¹H NMR (DMSO-d₆) δ (ppm) 12.39 (br. s, 1H), 8.44 (d, J=7.34 Hz, 1H), 8.33 (d, J=3.91 Hz, 1H), 8.08 (s, 1H), 7.43-7.53 (m, 2H), 7.33-7.43 (m, 2H), 7.23 (dd, J=4.89, 7.82 Hz, 1H), 6.91 (s, 1H), 5.37 (br. s, 2H), 5.17 (br. s, 2H), 4.23 (q, J=7.34 Hz, 2H), 1.29 (t, J=7.09 Hz, 3H); LCMS (m/z): 402.0 [M+H]⁺.

Example 106 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-4-oxo-2-[(1-phenylethyl)amino]-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 4-oxo-2-[(1-phenylethyl)amino]-4,5-dihydrofuran-3-carboxylate (0.40 g, 1.5 mmol) which similarly prepared according to the procedure described in the Example 4, First step and 7-azaindole-3-carboxaldehyde (0.21 g, 1.5 mmol) in ethanol (20 mL), L-proline (0.017 g, 0.15 mmol) was added at ambient temperature. The mixture was refluxed for 3 days. Cooled to ambient temperature, the precipitate was collected by filtration, washed with methanol then dried to afford the titled compound as solid (0.20 g, y. 34%).

¹H NMR (DMSO-d₆) δ (ppm) 12.35 (br. s, 1H), 8.97 (br. s, 1H), 8.25-8.34 (m, 2H), 7.82 (br. s, 1H), 7.49-7.57 (m, 2H), 7.38-7.45 (m, 2H), 7.24-7.33 (m, 1H), 7.12-7.19 (m, 1H), 6.86 (s, 1H), 5.41 (br. s, 1H), 4.24 (q, J=6.68 Hz, 2H), 1.70 (d, J=6.36 Hz, 3H), 1.27 (t, J=6.85 Hz, 3H); LCMS (m/z): 403.8 [M+H]⁺.

Example 107 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[3-(2,6-dimethylpyridinyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 106.

¹H NMR (DMSO-d₆) δ (ppm) 12.27 (br. s, 1H), 10.47 (s, 1H), 8.21 (d, J=3.91 Hz, 1H), 7.81 (d, J=7.82 Hz, 1H), 7.64-7.76 (m, 2H), 7.27 (d, J=7.83 Hz, 1H), 6.84 (s, 1H), 6.74 (dd, J=4.65, 7.58 Hz, 1H), 4.26 (q, J=7.17 Hz, 2H), 2.57 (s, 3H), 2.41 (s, 3H), 1.29 (t, J=7.09 Hz, 3H); LCMS (m/z): 405.0 [M+H]⁺.

Example 108 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[N-methyl-N-(2-thienylmethyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 106.

¹H NMR (DMSO-d₆) δ (ppm) 12.33 (br. s, 1H), 8.37 (d, J=7.82 Hz, 1H), 8.30 (d, J=3.91 Hz, 1H), 8.03 (s, 1H), 7.53 (d, J=4.89 Hz, 1H), 7.14-7.23 (m, 2H), 7.01-7.07 (m, 1H), 6.91 (s, 1H), 5.20 (s, 2H), 4.18 (q, J=7.34 Hz, 2H), 3.26 (br. s, 3H), 1.23 (t, J=7.09 Hz, 3H); LCMS (m/z): 409.8 [M+H]⁺.

Example 109 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-4-oxo-2-thiomorpholino-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 106.

¹H NMR (DMSO-d₆) δ (ppm) 12.33 (br. s, 1H), 8.27-8.45 (m, 2H), 7.94-8.04 (m, 1H), 7.20 (dd, J=4.65, 7.58 Hz, 1H), 6.88 (s, 1H), 4.17 (q, J=6.85 Hz, 2H), 4.02 (br. s, 4H), 2.89 (br. s, 4H), 1.25 (t, J=7.09 Hz, 3H); LCMS (m/z): 386.2 [M+H]⁺.

Example 110 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(3-hydroxypiperidino)-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 106.

¹H NMR (DMSO-d₆) δ (ppm) 12.32 (br. s, 1H), 8.39 (d, J=7.82 Hz, 1H), 8.31 (d, J=3.91 Hz, 1H), 7.93-8.00 (m, 1H), 7.20 (dd, J=4.40, 7.83 Hz, 1H), 6.85 (s, 1H), 5.11 (br. s, 1H), 4.17 (q, J=6.85 Hz, 2H), 3.85-3.94 (m, 1H), 3.72-3.84 (m, 2H), 3.54-3.65 (m, 1H), 3.41-3.53 (m, 1H), 1.83-2.02 (m, 2H), 1.51-1.69 (m, 2H), 1.25 (t, J=7.09 Hz, 3H); LCMS (m/z): 384.0 [M+H]⁺.

Example 111 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-({4-[2-(dimethylamino)ethoxy]-2-methylphenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate formate

To a solution of ethyl 2-({4-[2-(dimethylamino)ethoxy]-2-methylphenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.070 g, 0.20 mmol) which similarly prepared according to the procedure described in the Example 74, Fourth step and 7-azaindole-3-carboxaldehyde (0.029 g, 0.20 mmol) in ethanol (2.0 mL), piperidine (0.020 mL, 0.20 mmol) was added at ambient temperature. The mixture was refluxed for 5 days. Cooled to ambient temperature, the precipitate was collected by filtration then purified by preparative HPLC to afford the titled compound as solid (0.016 g, y. 16%).

¹H NMR (DMSO-d₆) δ (ppm) 12.28 (br. s, 1H), 10.53 (br. s, 1H), 8.18 (dd, J=1.63, 4.64 Hz, 1H), 8.16 (s, 1H), 7.76 (s, 1H), 7.67 (d, J=7.28 Hz, 1H), 7.41 (d, J=8.78 Hz, 1H), 7.04 (d, J=3.01 Hz, 1H), 6.94 (dd, J=2.89, 8.66 Hz, 1H), 6.82 (s, 1H), 6.75 (dd, J=4.77, 8.03 Hz, 1H), 4.26 (q, J=7.03 Hz, 2H), 4.16 (t, J=5.77 Hz, 2H), 2.74 (t, J=5.65 Hz, 2H), 2.30 (s, 6H), 2.28 (s, 3H), 1.29 (t, J=7.03 Hz, 3H); LCMS (m/z): 477.1 [M+H]⁺.

Example 112 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-4-oxo-2-[(2-phenyl-2-propinyl)amino]-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 4-oxo-2-[(2-phenyl-2-propinyl)amino]-4,5-dihydrofuran-3-carboxylate (0.40 g, 1.4 mmol) which similarly prepared according to the procedure described in the Example 4, First step and 7-azaindole-3-carboxaldehyde (0.20 g, 1.4 mmol) in ethanol (10 mL), L-proline (0.016 g, 0.14 mmol) was added at ambient temperature. The mixture was refluxed for 3 days. Cooled to ambient temperature, the precipitate was collected by filtration, washed with methanol then dried to afford the titled compound as solid (0.15 g, y. 26%).

¹H NMR (DMSO-d₆) δ (ppm) 12.22 (br. s, 1H), 8.92 (br. s, 1H), 8.25 (d, J=3.42 Hz, 1H), 8.10 (d, J=7.82 Hz, 1H), 7.58 (d, J=7.34 Hz, 2H), 7.44-7.53 (m, 2H), 7.23-7.33 (m, 1H), 7.06 (dd, J=4.65, 7.09 Hz, 1H), 6.90 (br. s, 1H), 6.78 (br. s, 1H), 4.27 (q, J=6.52 Hz, 2H), 1.85 (br. s, 6H), 1.29 (t, J=6.85 Hz, 3H); LCMS (m/z): 418.0 [M+H]⁺.

Example 113 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(2-adamantylamino)-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 112.

¹H NMR (DMSO-d₆) δ (ppm) 12.37 (br. s, 1H), 8.71 (d, J=6.85 Hz, 1H), 8.42 (d, J=7.82 Hz, 1H), 8.32 (d, J=3.91 Hz, 1H), 7.99 (s, 1H), 7.20 (dd, J=4.89, 7.83 Hz, 1H), 6.92 (s, 1H), 4.30-4.40 (m, 1H), 4.24 (q, J=7.01 Hz, 2H), 2.10-2.19 (m, 2H), 1.81-1.99 (m, 8H), 1.67-1.80 (m, 4H), 1.27 (t, J=7.09 Hz, 3H); LCMS (m/z): 433.8 [M+H]⁺.

Example 114 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[3-(6-methylpyridinyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 112.

¹H NMR (DMSO-d₆) δ (ppm) 12.23 (br. s, 1H), 8.41-8.61 (m, 2H), 8.25 (d, J=4.40 Hz, 1H), 8.02 (br. s, 1H), 7.79 (d, J=1.96 Hz, 1H), 7.50 (br. s, 1H), 6.89-7.00 (m, 1H), 6.78-6.89 (m, 1H), 4.08-4.28 (m, 2H), 2.27 (s, 3H), 1.18-1.30 (m, 3H); LCMS (m/z): 391.4 [M+H]⁺.

Example 115 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[4-(3-methylpyridinyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 112.

¹H NMR (DMSO-d₆) δ (ppm) 12.23 (br. s, 1H), 8.41-8.61 (m, 2H), 8.25 (d, J=4.40 Hz, 1H), 8.02 (br. s, 1H), 7.79 (d, J=1.96 Hz, 1H), 7.50 (br. s, 1H), 6.89-7.00 (m, 1H), 6.83 (br. s, 1H), 4.19 (br. s, 2H), 2.27 (s, 3H), 1.24 (br. s, 3H); LCMS (m/z): 391.4 [M+H]⁺.

Example 116 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(trans-4-hydroxycyclohexyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-[(trans-4-hydroxycyclohexyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.30 g, 1.1 mmol) which similarly prepared according to the procedure described in the Example 4, First step and 7-azaindole-3-carboxaldehyde (0.16 g, 1.1 mmol) in ethanol (20 mL), L-proline (0.013 g, 0.11 mmol) was added at ambient temperature. The mixture was refluxed for 2 days. Cooled to ambient temperature, the precipitate was collected by filtration, washed with methanol then dried to afford the titled compound as solid (0.14 g, y. 40%).

¹H NMR (DMSO-d₆) δ (ppm) 12.36 (br. s, 1H), 8.39-8.54 (m, 2H), 8.33 (d, J=4.40 Hz, 1H), 7.99 (s, 1H), 7.20 (dd, J=4.89, 7.83 Hz, 1H), 6.89 (s, 1H), 4.69 (d, J=3.91 Hz, 1H), 4.20 (q, J=7.34 Hz, 2H), 3.97 (br. s, 1H), 3.41-3.53 (m, 1H), 1.86-2.03 (m, 4H), 1.61-1.76 (m, 2H), 1.29-1.42 (m, 2H), 1.25 (t, J=7.09 Hz, 3H); LCMS (m/z): 398.1 [M+H]⁺.

Example 117 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[4-(2-fluoropyridinyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 116.

¹H NMR (DMSO-d₆) δ (ppm) 12.44 (br. s, 1H), 10.86 (br. s, 1H), 8.30 (d, J=5.38 Hz, 2H), 8.21 (d, J=7.82 Hz, 1H), 7.94 (d, J=1.96 Hz, 1H), 7.63 (d, J=5.38 Hz, 1H), 7.44 (s, 1H), 7.00-7.09 (m, 2H), 4.29 (q, J=7.01 Hz, 2H), 1.30 (t, J=7.09 Hz, 3H); LCMS (m/z): 395.0 [M+H]⁺.

Example 118 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(5-indazolyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 116.

¹H NMR (DMSO-d₆) δ (ppm) 13.36 (s, 1H), 12.25 (br. s, 1H), 10.62 (br. s, 1H), 8.17 (s, 1H), 8.09 (d, J=3.91 Hz, 1H), 7.99 (s, 1H), 7.78 (d, J=1.96 Hz, 1H), 7.63-7.72 (m, 2H), 7.52 (d, J=7.82 Hz, 1H), 6.85 (s, 1H), 6.12-6.27 (m, 1H), 4.28 (q, J=7.01 Hz, 2H), 1.30 (t, J=6.85 Hz, 3H); LCMS (m/z): 416.2 [M+H]⁺.

Example 119 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(5-benzimidazolyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-[(5-benzimidazolyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.30 g, 1.0 mmol) which similarly prepared according to the procedure described in the Example 74, Fourth step and 7-azaindole-3-carboxaldehyde (0.15 g, 1.0 mmol) in ethanol (5.0 mL), piperidine (0.20 mL, 3.1 mmol) was added at ambient temperature. The mixture was refluxed for 24 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with methanol then dried to afford the titled compound as solid (0.015 g, y. 4%).

¹H NMR (DMSO-d₆) δ (ppm) 12.28 (br. s, 1H), 10.82 (s, 1H), 9.55 (s, 1H), 8.16 (d, J=3.91 Hz, 1H), 8.08 (s, 1H), 7.98 (d, J=8.80 Hz, 1H), 7.87 (d, J=7.34 Hz, 1H), 7.59-7.89 (m, 1H), 7.74 (s, 1H), 6.93 (s, 1H), 6.53 (dd, J=4.89, 7.34 Hz, 1H), 4.30 (q, J=7.34 Hz, 2H), 1.31 (t, J=7.09 Hz, 3H); LCMS (m/z): 416.2 [M+H]⁺.

Example 120 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-indolinyl-4-oxo-4,5-dihydrofuran-3-carboxylate

First Step

To a solution of potassium tert-butoxide (0.56 g, 5.0 mmol) in tetrahydrofuran (5.0 mL) that cooled with ice bath, a solution of indoline (0.36 g, 3.0 mmol) in tetrahydrofuran (5.0 mL) was added dropwise. The mixture was stirred at ambient temperature for 30 min. Then the reaction mixture was cooled with ice bath, a solution of ethyl 2-ethoxy-4-oxo-4,5-dihydrofuran-3-carboxylate (0.50 g, 2.5 mmol) which afforded in the Example 74, Third step dissolved in tetrahydrofuran (5.0 mL) was added dropwise. The mixture was stirred at ambient temperature for further 16 h. The reaction mixture was poured into ice water, extracted with ethyl acetate for 3 times. The combined organic layer was dried over sodium sulfate and concentrated. The residue was purified by chromatography on silica gel. Eluted with hexane/ethyl acetate to afford 2-indolinyl-4-oxo-4,5-dihydrofuran-3-carboxylate as solid (0.095 g, y. 8%).

¹H NMR (DMSO-d₆) δ (ppm) 7.49 (d, J=8.00 Hz, 1H), 7.35 (d, J=7.60 Hz, 1H), 7.24 (t, J=7.46 Hz, 1H), 7.13 (t, J=7.46 Hz, 1H), 4.73 (s, 2H), 4.05-4.20 (m, 4H), 3.20 (t, J=8.12 Hz, 2H), 1.21 (t, J=7.08 Hz, 3H); LCMS (m/z): 274.0 [M+H]⁺.

Second Step

To a solution of 2-indolinyl-4-oxo-4,5-dihydrofuran-3-carboxylate (0.090 g, 0.32 mmol) and 7-azaindole-3-carboxaldehyde (0.048 g, 0.32 mmol) in ethanol (5.0 mL), L-proline (0.0040 g, 0.030 mmol) was added at ambient temperature. The mixture was refluxed for 2 days. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol then dried to afford the titled compound as solid (0.076 g, y. 58%).

¹H NMR (DMSO-d₆) δ (ppm) 12.40 (br. s, 1H), 8.36 (d, J=6.85 Hz, 1H), 8.31 (d, J=3.91 Hz, 1H), 8.01 (s, 1H), 7.56-7.63 (m, 1H), 7.44 (d, J=7.34 Hz, 1H), 7.33 (t, J=7.58 Hz, 1H), 7.21-7.27 (m, 1H), 7.12 (dd, J=4.89, 7.83 Hz, 1H), 7.00 (s, 1H), 4.26 (t, J=7.82 Hz, 2H), 4.19 (q, J=7.17 Hz, 2H), 3.24-3.28 (m, 2H), 1.22 (t, J=7.09 Hz, 3H); LCMS (m/z): 402.0 [M+H]⁺.

Example 121 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(1,1-dioxidothiomorpholino)-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-(1,1-dioxidothiomorpholino)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.027 g, 0.092 mmol) which similarly prepared according to the procedure described in the Example 74, Fourth step and 7-azaindole-3-carboxaldehyde (0.014 g, 0.092 mmol) in ethanol (0.5 mL), piperidine (0.00092 mL, 0.0093 mmol) was added at ambient temperature. The mixture was refluxed for 2 days. The precipitate was collected by filtration, washed with hot ethanol. The solid was washed with hexane then dried to afford the titled compound as solid (0.037 g, y. 47%).

¹H NMR (DMSO-d₆) δ (ppm) 12.40 (br. s, 1H), 8.38 (d, J=8.03 Hz, 1H), 8.32 (dd, J=1.51, 4.77 Hz, 1H), 8.03 (d, J=2.51 Hz, 1H), 7.22 (dd, J=4.64, 7.91 Hz, 1H), 6.94 (s, 1H), 4.12-4.25 (m, 6H), 3.47-3.55 (m, 4H), 1.25 (t, J=7.03 Hz, 3H); LCMS (m/z): 418.0 [M+H]⁺.

Example 122 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[3-(hydroxymethyl)piperidino]-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-[(3-hydroxymethyl)piperidino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.30 g, 1.0 mmol) which similarly prepared according to the procedure described in the Example 74, Fourth step and 7-azaindole-3-carboxaldehyde (0.16 g, 1.0 mmol) in ethanol (5.0 mL), L-proline (0.0040 g, 0.030 mmol) was added at ambient temperature. The mixture was refluxed for 2 days. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol then dried to afford the titled compound as solid (0.045 g, y. 10%).

¹H NMR (DMSO-d₆) δ (ppm) 12.33 (br. s, 1H), 8.38 (d, J=7.82 Hz, 1H), 8.31 (d, J=4.40 Hz, 1H), 7.98 (s, 1H), 7.19 (dd, J=4.89, 7.82 Hz, 1H), 6.84 (s, 1H), 4.72 (br. s, 1H), 4.12-4.24 (m, 2H), 3.24-3.50 (m, 4H), 3.17 (t, J=11.74 Hz, 2H), 1.74-1.91 (m, 2H), 1.60-1.72 (m, 2H), 1.29-1.41 (m, 1H), 1.24 (t, J=6.85 Hz, 3H); LCMS (m/z): 398.2 [M+H]⁺.

Example 123 2-(Dimethylamino)ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-azepinyl-4-oxo-4,5-dihydrofuran-3-carboxylate formate

A solution of the compound (0.052 g, 0.14 mmol) of Example 63, 2-dimethylaminoethanol (0.14 mL, 1.4 mmol), zinc cluster catalyst (Zn₄(OCOCF₃)₆O) (0.0019 g, 0.0020 mmol) and 4-dimethylaminopyridine (0.0049 g, 0.040 mmol) in N,N-dimethylacetamide (1.0 mL) was stirred with the microwave synthesizer (Biotage Initiator™) at 150° C. for 1 h. The reaction mixture was purified by preparative HPLC to afford the titled compound as solid (0.018 g, y. 24%).

¹H NMR (DMSO-d₆) δ (ppm) 12.31 (br. s, 1H), 8.39 (dd, J=1.25, 7.78 Hz, 1H), 8.31 (dd, J=1.38, 4.64 Hz, 1H), 8.16 (s, 1H), 7.94 (d, J=1.76 Hz, 1H), 7.20 (dd, J=4.77, 7.78 Hz, 1H), 6.86 (s, 1H), 4.22 (t, J=5.77 Hz, 2H), 3.72-3.92 (m, 4H), 2.54-2.60 (m, 2H), 2.24 (s, 6H), 1.78-1.90 (m, 4H), 1.52-1.63 (m, 4H); LCMS (m/z): 425.1 [M+H]⁺.

Example 124 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{[2-(3-methylthienyl)methyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-{[2-(3-methylthienyl)methyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate (0.38 g, 1.4 mmol) which similarly prepared according to the procedure described in the Example 74, Fourth step and 7-azaindole-3-carboxaldehyde (0.20 g, 1.4 mmol) in ethanol (4.0 mL), piperidine (0.014 mL, 0.14 mmol) was added at ambient temperature. The mixture was refluxed for 5 days. The precipitate was collected by filtration, washed with hot ethanol. The solid was washed with hexane then dried to afford the titled compound as solid (0.12 g, y. 21%).

¹H NMR (DMSO-d₆) δ (ppm) 12.38 (br. s, 1H), 9.47 (t, J=6.27 Hz, 1H), 8.35 (d, J=8.03 Hz, 1H), 8.30 (dd, J=1.38, 4.64 Hz, 1H), 7.97 (d, J=2.26 Hz, 1H), 7.32 (d, J=5.02 Hz, 1H), 7.15 (dd, J=4.64, 7.91 Hz, 1H), 6.92 (s, 1H), 6.88 (d, J=5.02 Hz, 1H), 4.95 (d, J=6.27 Hz, 2H), 4.22 (q, J=7.03 Hz, 2H), 2.28 (s, 3H), 1.26 (t, J=7.03 Hz, 3H); LCMS (m/z): 410.0 [M+H]⁺.

Example 125 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-({4-[(2-hydroxyethyl)-N-methylamino]-2-methylphenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate

First Step

A solution of 5-fluoro-2-nitrotoluene (1.5 g, 9.6 mmol), N-methylethanolamine (0.90 mL, 0.012 mol) and triethylamine (1.6 mL, 0.012 mol) in N-methyl-2-pyrrolidone (15 mL) was stirred at 60° C. for 12 h. Cooled to ambient temperature, the reaction mixture was poured into ice water, extracted with ethyl acetate. The organic layer was washed with water and brine, dried over sodium sulfate and concentrated to afford 2-[(3-methyl-4-nitrophenyl)-N-methylamino]ethanol as solid (2.0 g, y. 98%).

¹H NMR (DMSO-d₆) δ (ppm) 7.99 (d, J=9.32 Hz, 1H), 6.58-6.71 (m, 2H), 4.78 (t, J=5.22 Hz, 1H), 3.46-3.63 (m, 4H), 3.06 (s, 3H), 2.56 (s, 3H); LCMS (m/z): 211.2 [M+H]⁺.

Second Step

2-[(3-Methyl-4-nitrophenyl)-N-methylamino]ethanol (0.70 g, 3.3 mmol) was dissolved in ethanol (10 mL) and 10% palladium on carbon (0.050 g) was added at ambient temperature. The reaction mixture was agitated under a hydrogen atmosphere at ambient temperature for 4 h. Palladium on carbon was removed by filtration with Celite and the solvent was removed under reduced pressure to afford 2-[(4-amino-3-methylphenyl)-N-methylamino]ethanol as solid (0.58 g, y. 97%).

¹H NMR (DMSO-d₆) δ (ppm) 6.49 (d, J=8.44 Hz, 1H), 6.43 (d, J=2.60 Hz, 1H), 6.38 (q, J=6.13 Hz, 1H), 4.50 (t, J=5.38 Hz, 1H), 4.12 (br. s, 2H), 3.48 (dd, J=6.38, 12.00 Hz, 2H), 3.16 (t, J=6.58 Hz, 2H), 2.73 (s, 3H), 2.02 (s, 3H); LCMS (m/z): 181.1 [M+H]⁺.

Third Step

A solution of ethyl 2-ethoxy-4-oxo-4,5-dihydrofuran-3-carboxylate (0.70 g, 3.5 mmol) which afforded in the Example 74, Third step and 2-[(4-amino-3-methylphenyl)-N-methylamino]ethanol (0.60 g, 3.5 mmol) in ethanol (8.3 mL) was stirred at ambient temperature for 16 h. The solvent was removed under reduced pressure, and ethanol was added to precipitate the product. The precipitate was collected by filtration, washed with ethanol then dried to afford 2-({4-[(2-hydroxyethyl)-N-methylamino]-2-methylphenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate as solid (0.60 g, y. 60%).

¹H NMR (DMSO-d₆) δ (ppm) 9.86 (br. s, 1H), 7.12 (d, J=8.76 Hz, 1H), 6.60 (d, J=2.60 Hz, 1H), 5.54 (dd, J=2.74, 8.80 Hz, 1H), 4.65 (br. s, 1H), 4.57 (s, 2H), 4.20 (q, J=7.06 Hz, 2H), 3.53 (t, J=6.14 Hz, 2H), 3.38 (t, J=6.22 Hz, 2H), 2.92 (s, 3H), 2.17 (s, 3H); LCMS (m/z): 335.0 [M+H]⁺.

Fourth Step

To a solution of 2-({4-[(2-hydroxyethyl)-N-methylamino]-2-methylphenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.20 g, 0.60 mmol) and 7-azaindole-3-carboxaldehyde (0.097 g, 0.60 mmol) in ethanol (11 mL), piperidine (0.10 mL, 1.0 mmol) was added at ambient temperature. The mixture was refluxed for 2 days. Cooled to ambient temperature, the precipitate was collected by filtration, washed with diethyl ether then dried to afford the titled compound as solid (0.055 g, y. 19%).

¹H NMR DMSO-d₆) δ (ppm) 12.22 (br. s, 1H), 10.12 (br. s, 1H), 8.15 (d, J=4.40 Hz, 1H), 7.77 (br. s, 1H), 7.68 (d, J=7.82 Hz, 1H), 7.22 (d, J=8.80 Hz, 1H), 6.77 (s, 1H), 6.67-6.73 (m, 2H), 6.63 (d, J=8.31 Hz, 1H), 4.69 (t, J=5.14 Hz, 1H), 4.23 (q, J=6.85 Hz, 2H), 3.55-3.63 (m, 2H), 3.43-3.50 (m, 2H), 3.01 (s, 3H), 2.15 (s, 3H), 1.26 (t, J=6.85 Hz, 3H); LCMS (m/z): 463.2 [M+H]⁺.

Example 126 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-({4-[(2-hydroxyethyl)amino]-2-methylphenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 125.

¹H NMR (DMSO-d₆) δ (ppm) 12.25 (br. s, 1H), 10.11 (s 1H), 8.18 (d, J=4.40 Hz, 1H), 7.81-7.87 (m, 1H), 7.70 (d, J=7.83 Hz, 1H), 7.16 (d, J=8.31 Hz, 1H), 6.75-6.87 (m, 2H), 6.53-6.67 (m, 2H), 5.85 (t, J=5.38 Hz, 1H), 4.75 (t, J=5.38 Hz, 1H), 4.26 (q, J=6.85 Hz, 2H), 3.63 (q, J=5.87 Hz, 2H), 3.20 (q, J=5.71 Hz, 2H), 2.13 (s, 3H), 1.29 (t, J=7.09 Hz, 3H); LCMS (m/z): 449.2 [M+H]⁺.

Example 127 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{[4-(2,3-dihydroxypropoxy)-2-methylphenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate

To a stirred solution of ethyl 2-{[4-(2,3-dihydroxypropoxy)-2-methylphenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate (0.17 g, 0.50 mmol) which similarly prepared according to the procedure described in the Example 74, First step to Fourth step and 7-azaindole-3-carboxaldehyde (0.070 g, 0.50 mmol) in ethanol (5.0 mL), piperidine (0.10 mL, 1.0 mmol) was added at ambient temperature. The mixture was refluxed for 2 days. Cooled to ambient temperature, the precipitate was collected by filtration, washed with diethyl ether then dried to afford the titled compound as solid (0.054 g, y. 23%).

¹H NMR (DMSO-d₆) δ (ppm) 12.28 (br. s, 1H), 10.30 (br. s, 1H), 8.18 (d, J=2.93 Hz, 1H), 7.78 (br. s, 1H), 7.64 (d, J=7.34 Hz, 1H), 7.41 (d, J=8.31 Hz, 1H), 7.03 (br. s, 1H), 6.94 (d, J=7.34 Hz, 1H), 6.82 (s, 1H), 6.70-6.79 (m, 1H), 5.06 (d, J=4.40 Hz, 1H), 4.76 (t, J=5.04 Hz, 1H), 4.20-4.32 (m, 2H), 4.05-4.15 (m, 1H), 3.93-4.02 (m, 1H), 3.83-3.92 (m, 1H), 3.47-3.57 (m, 2H), 2.22 (br. s, 3H), 1.29 (t, J=6.60 Hz, 3H); LCMS (m/z): 480.2 [M+H]⁺.

Example 128 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-({2-methyl-4-[2-(methylthio)ethoxy]phenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 127.

¹H NMR (DMSO-d₆) δ (ppm) 12.28 (br. s, 1H), 10.30 (br. s, 1H), 8.19 (d, J=4.40 Hz, 1H), 7.77 (s, 1H), 7.65 (d, J=7.82 Hz, 1H), 7.42 (d, J=8.80 Hz, 1H), 7.05 (br. s, 1H), 6.95 (dd, J=2.69, 8.56 Hz, 1H), 6.82 (br. s, 1H), 6.76 (dd, J=4.40, 7.83 Hz, 1H), 4.21-4.32 (m, 4H), 2.94 (t, J=6.36 Hz, 2H), 2.23 (s, 3H), 2.22 (s, 3H), 1.29 (t, J=7.09 Hz, 3H); LCMS (m/z): 480.2 [M+H]⁺.

Example 129 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-({4-[(dimethylamino)methyl]-2-methylphenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate

First Step

A solution of 3-methyl-4-nitrobenzyl bromide (1.0 g, 4.3 mmol), anhydrous dimethylamine (0.34 mL, 5.1 mmol) and potassium carbonate (1.5 g, 0.011 mol) in N,N-dimethylformamide (4.5 mL) was stirred at 70° C. for 12 h. Cooled to ambient temperature, the reaction mixture was poured into ice water, extracted with ethyl acetate. The organic layer was washed with water and brine, dried over sodium sulfate and concentrated to afford N,N-dimethyl-1-(3-methyl-4-nitrophenyl)methanamine as oil (0.80 g, y. 95%).

¹H NMR (DMSO-d₆) δ (ppm) 7.65 (d, J=8.32 Hz, 1H), 7.41 (s, 1H), 7.36 (d, J=8.36 Hz, 1H), 3.44 (s, 2H), 2.52 (s, 3H), 2.15 (s, 6H); LCMS (m/z): 195.4 [M+H]⁺.

Second Step

N,N-Dimethyl-1-(3-methyl-4-nitrophenyl)methanamine (0.80 g, 4.1 mmol) was dissolved in ethanol (5.0 mL) and 10% palladium on carbon (0.18 g) was added at ambient temperature. The reaction mixture was agitated under a hydrogen atmosphere at ambient temperature for 4 h. Palladium on carbon was removed by filtration with Celite and the solvent was removed under reduced pressure to afford 4-[(dimethylamino)methyl]-2-methylaniline (0.65 g, y. 96%).

¹H NMR (DMSO-d₆) δ (ppm) 6.81 (s, 1H), 6.77 (d, J=7.92 Hz, 1H), 6.52 (d, J=7.96 Hz, 1H), 4.68 (s, 2H), 3.16 (s, 2H), 2.07 (s, 6H), 2.02 (s, 3H).

Third Step

A solution of ethyl 2-ethoxy-4-oxo-4,5-dihydrofuran-3-carboxylate (0.47 g, 2.3 mmol) which afforded in the Example 74, Third step and 4-[(dimethylamino)methyl]-2-methylaniline (0.35 g, 2.1 mmol) in ethanol (10 mL) was stirred at ambient temperature for 16 h. The solvent was removed under reduced pressure, and ethanol was added to precipitate the product. The precipitate was collected by filtration to afford ethyl 2-({4-[(dimethylamino)methyl]-2-methylphenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.26 g, crude material) as solid.

¹H NMR (DMSO-d₆) δ (ppm) 10.18 (br. s, 1H), 7.48 (d, J=6.96 Hz, 1H), 7.25-7.40 (m, 2H), 4.65 (s, 2H), 4.23 (q, J=7.04 Hz, 2H), 2.40-2.60 (m, 9H), 1.26 (t, J=7.04 Hz, 3H); LCMS (m/z): 319.2 [M+H]⁺.

Fourth Step

To a solution of ethyl 2-({4-[(dimethylamino)methyl]-2-methylphenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.26 g, 0.82 mmol) and 7-azaindole-3-carboxaldehyde (0.12 g, 0.82 mmol) in ethanol (5.0 mL), piperidine (0.16 mL, 1.6 mmol) was added at ambient temperature. The mixture was refluxed for 2 days. Cooled to ambient temperature, the precipitate was collected by filtration, washed with diethyl ether then dried to afford the titled compound as solid (0.015 g, y. 4%).

¹H NMR (DMSO-d₆) δ (ppm) 12.26 (br. s, 1H), 8.20 (d, J=3.91 Hz, 1H), 8.15 (s, 1H), 7.82 (d, J=7.34 Hz, 1H), 7.72 (s, 1H), 7.47 (d, J=7.83 Hz, 1H), 7.37 (s, 1H), 7.30 (d, J=8.31 Hz, 1H), 6.83 (s, 1H), 6.79 (dd, J=4.89, 7.82 Hz, 1H), 4.26 (q, J=7.17 Hz, 2H), 3.54 (s, 2H), 2.22-2.29 (m, 9H), 1.29 (t, J=6.85 Hz, 3H); LCMS (m/z): 447.4 [M+H]⁺

Example 130 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-4-oxo-2-{[(1R,2S)-2-phenylcyclopropyl]amino}-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 4-oxo-2-{[(1R,2S)-2-phenylcyclopropyl]amino}-4,5-dihydrofuran-3-carboxylate (0.090 g, 0.31 mmol) which similarly prepared according to the procedure described in the Example 74, Fourth step and 7-azaindole-3-carboxaldehyde (0.045 g, 0.30 mmol) in ethanol (5.0 mL), L-proline (0.0035 g, 0.031 mmol) was added at ambient temperature. The mixture was refluxed for 2 days. Cooled to ambient temperature, the solvent was removed under reduced pressure then the residue was purified by preparative HPLC to afford the titled compound as solid (0.018 g, y. 14%).

¹H NMR (DMSO-d₆) δ (ppm) 12.11 (br. s, 1H), 9.10 (br. s, 1H), 8.41 (d, J=7.34 Hz, 1H), 8.26 (d, J=3.91 Hz, 1H), 7.81 (s, 1H), 7.28-7.36 (m, 2H), 7.19-7.27 (m, 3H), 7.03 (dd, J=4.40, 7.82 Hz, 1H), 6.88 (s, 1H), 4.21 (q, J=6.85 Hz, 2H), 3.40-3.48 (m, 1H), 2.88-3.10 (m, 1H), 1.63-1.72 (m, 1H), 1.35-1.43 (m, 1H), 1.26 (t, J=7.08 Hz, 3H); LCMS (m/z): 415.8 [M+H]⁺.

Example 131 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-({4-[2-(2-hydroxyethoxy)ethoxy]-2-methylphenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-({4-[2-(2-hydroxyethoxy)ethoxy]-2-methylphenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.15 g, 0.50 mmol) which similarly prepared according to the procedure described in the Example 74, First step to Fourth step and 7-azaindole-3-carboxaldehyde (0.070 g, 0.50 mmol) in ethanol (10 mL), L-proline (0.010 g, 0.087 mmol) was added at ambient temperature. The mixture was refluxed for 2 days. Cooled to ambient temperature, the precipitate was collected by filtration, washed with diethyl ether then dried to afford the titled compound (0.031 g, y. 15%) as solid.

¹H NMR (DMSO-d₆) δ (ppm) 12.28 (br. s, 1H), 10.29 (br. s, 1H), 8.20 (d, J=3.91 Hz, 1H), 7.75 (br. s, 1H), 7.68 (d, J=7.82 Hz, 1H), 7.42 (d, J=8.80 Hz, 1H), 7.05 (br. s, 1H), 6.95 (dd, J=2.40, 8.60 Hz, 1H), 6.83 (s, 1H), 6.77 (dd, J=4.40, 7.83 Hz, 1H), 4.64-4.70 (m, 1H), 4.26 (q, J=6.85 Hz, 2H), 4.17-4.23 (m, 2H), 3.79-3.87 (m, 2H), 3.52-3.62 (m, 4H), 2.23 (s, 3H), 1.29 (t, J=6.85 Hz, 3H); LCMS (m/z): 494.0 [M+H]⁺.

Example 132 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{[4-(3-hydroxypropoxy)-2-methylphenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 131.

¹H NMR (DMSO-d₆) δ (ppm) 12.25 (br. s, 1H), 8.19 (d, J=4.40 Hz, 1H), 7.74 (s, 1H), 7.67 (d, J=7.82 Hz, 1H), 7.36 (d, J=8.80 Hz, 1H), 7.00 (d, J=2.4 Hz, 1H), 6.91 (d, J=8.31 Hz, 1H), 6.71-6.79 (m, 2H), 4.59-4.65 (m, 1H), 4.25 (q, J=6.85 Hz, 2H), 4.14 (t, J=6.11 Hz, 2H), 3.60-3.67 (m, J=5.38 Hz, 2H), 2.21 (s, 3H), 1.94 (t, J=6.11 Hz, 2H), 1.28 (t, J=7.06 Hz, 3H); LCMS (m/z): 464.2 [M+H]⁺.

Example 133 Ethyl 2-({4-[2-(1H-pyrrol-1-yl)ethoxy]-2-methylphenyl}amino)-5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 131.

¹H NMR (DMSO-d₆) δ (ppm) 12.04 (br. s, 1H), 8.08 (d, J=3.91 Hz, 1H), 7.60-7.76 (m, 2H), 7.16 (br. s, 1H), 6.88-6.94 (m, 3H), 6.84 (d, J=7.82 Hz, 1H), 6.60-6.68 (m, 1H), 6.48-6.57 (m, 1H), 6.02-6.07 (m, 2H), 4.22-4.37 (m, 4H), 4.13-4.22 (m, 2H), 2.14 (s, 3H), 1.25 (t, J=6.85 Hz, 3H); LCMS (m/z): 499.2 [M+H]⁺.

Example 134 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{[2-methyl-4-(1-pyrrolidinylmethyl)phenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of 2-{[2-methyl-4-(1-pyrrolidinylmethyl)phenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate (0.25 g, 0.70 mmol) which similarly prepared according to the procedure described in the Example 129, First step to Third step and 7-azaindole-3-carboxaldehyde (0.10 g, 0.70 mmol) in ethanol (10 mL), piperidine (0.014 mL, 0.14 mmol) was added at ambient temperature. The mixture was refluxed for 2 days. Cooled to ambient temperature then the reaction mixture was purified by preparative HPLC to afford the titled compound as solid (0.035 g, y. 10%).

¹H NMR (DMSO-d₆) δ (ppm) 12.27 (br. s, 1H), 8.20 (d, J=3.91 Hz, 1H), 8.14 (s, 1H), 7.72-7.82 (m, 2H), 7.48 (d, J=7.83 Hz, 1H), 7.41 (s, 1H), 7.34 (d, J=7.34 Hz, 1H), 6.76-6.86 (m, 2H), 4.26 (q, J=6.85 Hz, 2H), 3.81 (br. s, 2H), 2.57-2.69 (m, 4H), 2.27 (s, 3H), 1.70-1.85 (m, 4H), 1.29 (t, J=6.85 Hz, 3H); LCMS (m/z): 473.2 [M+H]⁺.

Example 135 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-({2-methyl-4-[(4-methylpiperazinyl)methyl]phenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 134.

¹H NMR (DMSO-d₆) δ (ppm) 12.43 (br. s, 1H), 8.18 (br. s, 1H), 8.01 (br. s, 1H), 7.41 (br. s, 1H), 6.99-7.17 (m, 3H), 6.87 (br. s, 2H), 6.40 (d, J=12.72 Hz, 1H), 4.26 (br. s, 2H), 3.57 (br. s, 2H), 2.42 (br. s, 8H), 2.28 (br. s, 3H), 2.08 (br. s, 3H), 1.12-1.39 (m, 3H); LCMS (m/z): 502.4 [M+H]⁺.

Example 136 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{[2-methyl-4-(morpholinomethyl)phenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 134.

¹H NMR (DMSO-d₆) δ (ppm) 12.29 (br. s, 1H), 10.38 (br. s, 1H), 8.21 (d, J=4.40 Hz, 1H), 7.72-7.83 (m, 2H), 7.46-7.54 (m, 1H), 7.28-7.44 (m, 2H), 6.86 (s, 1H), 6.75-6.81 (m, 1H), 4.27 (q, J=7.17 Hz, 2H), 3.51-3.74 (m, 6H), 2.35-2.59 (m, 4H), 2.27 (s, 3H), 1.30 (t, J=7.09 Hz, 3H); LCMS (m/z): 489.2 [M+H]⁺.

Example 137 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-{[2-(dimethylamino)ethyl]amino}-2-methylphenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate formate

To a solution of ethyl 2-[(4-{[2-(dimethylamino)ethyl]amino}-2-methylphenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.27 g, 0.70 mmol) which similarly prepared according to the procedure described in the Example 125, First step to Third step and 7-azaindole-3-carboxaldehyde (0.11 g, 0.70 mmol) in ethanol (5.0 mL), piperidine (0.10 mL, 1.0 mmol) was added at ambient temperature. The mixture was refluxed for 16 h. Cooled to ambient temperature, the solvent was removed under reduced pressure then the residue was purified by preparative HPLC to afford the titled compound as solid (0.015 g, y. 8%).

¹H NMR (DMSO-d₆) δ (ppm) 12.29 (br. s, 1H), 10.12 (br. s, 1H), 8.11-8.23 (m, 2H), 7.86 (s, 1H), 7.67 (d, J=7.82 Hz, 1H), 7.17 (d, J=8.31 Hz, 1H), 6.75-6.83 (m, 2H), 6.51-6.69 (m, 2H), 5.79 (br. s, 1H), 4.25 (q, J=6.85 Hz, 2H), 3.16-3.28 (m, 2H), 2.58 (t, J=6.11 Hz, 2H), 2.29 (s, 6H), 2.13 (s, 3H), 1.29 (t, J=6.85 Hz, 3H); LCMS (m/z): 476.0 [M+H]⁺.

Example 138 2-Methoxyethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-azepinyl-4-oxo-4,5-dihydrofuran-3-carboxylate

First Step

A solution of ethyl malonyl chloride (0.10 mL, 0.78 mmol) and 2-methoxyethanol (0.061 mL, 0.78 mmol) in dichloromethane (1.5 mL) was cooled with ice bath. To this solution, triethylamine (0.13 mL, 0.94 mmol) was added and stirred at ambient temperature for 1.5 h. The reaction mixture was diluted with water, extracted with dichloromethane. The organic layer was washed with brine, dried over magnesium sulfate and concentrated. The residue was purified by chromatography on silica gel(hexane/ethyl acetate) to afford ethyl 2-methoxyethyl malonate (0.10 g, y. 69%) as oil.

¹H NMR (CDCl₃) δ (ppm) 4.28-4.34 (m, 2H), 4.21 (q, J=7.11 Hz, 2H), 3.59-3.64 (m, 2H), 3.42 (s, 2H), 3.39 (s, 3H), 1.29 (t, J=7.15 Hz, 3H)

Second Step

A solution of ethyl 2-methoxyethyl malonate (0.10 g, 0.53 mmol) in anhydrous tetrahydrofuran (1.5 mL) was added dropwise to a solution of sodium hydride (60% w/w in oil, 0.043 g, 1.1 mmol) in anhydrous tetrahydrofuran (2.0 mL) that cooled with ice bath. The mixture was refluxed for 5 min. The reaction mixture was cooled with ice bath, chloroacetyl chloride (0.042 mL, 0.53 mmol) was added dropwise to the reaction mixture and the mixture was stirred for 1 h then stirred with heating at 45° C. for 1 h. The reaction mixture was cooled to ambient temperature then hexamethyleneimine (0.071 mL, 0.63 mmol) was added dropwise and the mixture was stirred at ambient temperature for further 1 h. The reaction mixture was diluted with water, and extracted with chloroform. The organic layer was dried over magnesium sulfate and concentrated. The residue was purified by chromatography on silica gel, eluted with chloroform/methanol to afford 2-methoxyethyl 2-azepinyl-4-oxo-4,5-dihydrofuran-3-carboxylate as oil (0.065 g, y. 43%).

¹H NMR (CDCl₃) δ (ppm) 4.51 (s, 2H), 4.35-4.39 (m, 2H), 3.56-3.75 (m, 6H), 3.40 (s, 3H), 1.80 (br. s, 4H), 1.61 (br. s, 4H); LCMS (m/z): 284.1 [M+H]⁺.

Third Step

To a solution of 2-methoxyethyl 2-azepinyl-4-oxo-4,5-dihydrofuran-3-carboxylate (0.065 g, 0.23 mmol) and 7-azaindole-3-carboxaldehyde (0.033 g, 0.23 mmol) in ethanol (0.5 mL), hexamethyleneimine (0.026 mL, 0.023 mmol) was added at ambient temperature. The mixture was refluxed for 3 days. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol and hexane then dried to afford the titled compound as solid (0.019 g, y. 20%).

¹H NMR (DMSO-d₆) δ (ppm) 12.31 (br. s, 1H), 8.39 (dd, J=1.38, 7.91 Hz, 1H), 8.31 (dd, J=1.38, 4.64 Hz, 1H), 7.94 (s, 1H), 7.20 (dd, J=4.64, 7.91 Hz, 1H), 6.85 (s, 1H), 4.21-4.28 (m, 2H), 3.70-3.90 (m, 4H), 3.54-3.62 (m, 2H), 3.29 (s, 3H), 1.78-1.91 (m, 4H), 1.51-1.63 (m, 4H); LCMS (m/z): 412.1 [M+H]⁺.

Example 139 Isopropyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-azepinyl-4-oxo-4,5-dihydrofuran-3-carboxylate

To a stirred solution of isopropyl 2-azepinyl-4-oxo-4,5-dihydrofuran-3-carboxylate (0.033 g, 0.12 mmol) which similarly prepared according to the procedure described in the Example 4, First step and 7-azaindole-3-carboxaldehyde (0.019 g, 0.13 mmol) in 2-propanol (1.0 mL), hexamethyleneimine (0.0014 mL, 0.012 mmol) was added at ambient temperature. The mixture was refluxed for 12 days. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC to afford the titled compound as solid (0.024 g, y. 49%).

¹H NMR (DMSO-d₆) δ (ppm) 12.30 (br. s, 1H), 8.37 (dd, J=1.38, 7.91 Hz, 1H), 8.31 (dd, J=1.38, 4.64 Hz, 1H), 7.93 (s, 1H), 7.20 (dd, J=4.77, 7.78 Hz, 1H), 6.81 (s, 1H), 4.94-5.05 (m, 1H), 3.70-3.90 (m, 4H), 1.75-1.91 (m, 4H), 1.52-1.62 (m, 4H), 1.25 (d, J=6.27 Hz, 6H); LCMS (m/z): 396.1 [M+H]⁺.

Example 140 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{[2-methyl-4-(2-pyridinylmethoxy)phenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound was similarly prepared as solid according to the procedure described in the Example 1, using ethyl 2-{[2-methyl-4-(2-pyridinylmethoxy)phenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate which similarly prepared according to the procedure described in the Example 74, First step to Fourth step and 7-azaindole-3-carboxaldehyde as starting materials.

¹H NMR (DMSO-d₆) δ (ppm) 12.29 (br. s, 1H), 10.30 (br. s, 1H), 8.63 (d, J=3.91 Hz, 1H), 8.17 (d, J=3.91 Hz, 1H), 7.89 (t, J=7.09 Hz, 1H), 7.67-7.79 (m, 2H), 7.62 (d, J=7.34 Hz, 1H), 7.34-7.49 (m, 2H), 7.16 (br. s, 1H), 7.04 (d, J=8.31 Hz, 1H), 6.83 (s, 1H), 6.70-6.77 (m, 1H), 5.29 (s, 2H), 4.26 (q, J=6.85 Hz, 2H), 2.23 (s, 3H), 1.29 (t, J=7.09 Hz, 3H); LCMS (m/z): 497.0 [M+H]⁺.

Example 141 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{[2-methyl-4-(3-pyridinylmethoxy)phenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-{[2-methyl-4-(3-pyridinylmethoxy)phenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate (0.080 g, 0.20 mmol) which similarly prepared according to the procedure described in the Example 74, First step to Fourth step and 7-azaindole-3-carboxaldehyde (0.032 g, 0.20 mmol) in ethanol (5.0 mL), L-proline (0.0050 g, 0.043 mmol) was added at ambient temperature. The mixture was refluxed for 2 days. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC to afford the titled compound as solid (0.010 g, y. 9%).

¹H NMR (DMSO-d₆) δ (ppm) 12.25 (br. s, 1H), 10.25 (br. s, 1H), 8.76 (s, 1H), 8.59 (d, J=3.42 Hz, 1H), 8.18 (d, J=4.40 Hz, 1H), 7.96 (d, J=7.83 Hz, 1H), 7.69-7.79 (m, 2H), 7.45-7.53 (m, 1H), 7.41 (d, J=8.80 Hz, 1H), 7.14 (br. s, 1H), 7.04 (d, J=8.31 Hz, 1H), 6.79 (br. s, 1H), 6.68-6.76 (m, 1H), 5.27 (s, 2H), 4.20-4.30 (m, 2H), 2.23 (s, 3H), 1.29 (t, J=7.09 Hz, 3H); LCMS (m/z): 497.4 [M+H]⁺.

Example 142 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(morpholinoamino)-4-oxo-4,5-dihydrofuran-3-carboxylate

A solution of ethyl 2-ethoxy-4-oxo-4,5-dihydrofuran-3-carboxylate (0.10 g, 0.50 mmol) which afforded in the Example 74, Third step and 4-aminomorpholine (0.046 mL, 0.48 mmol) in ethanol (2.0 mL) was stirred at ambient temperature for 15 h. To this reaction mixture, 7-azaindole-3-carboxaldehyde (0.061 g, 0.42 mmol) and piperidine (0.0048 mL, 0.048 mmol) were added. The mixture was refluxed for 3 days. The precipitate was collected by filtration, washed with hot ethanol. The solid was washed with hexane then dried to afford the titled compound (0.045 g, y. 24%) as solid.

¹H NMR (DMSO-d₆) δ (ppm) 12.34 (br. s, 1H), 10.07 (s, 1H), 8.93 (d, J=7.78 Hz, 1H), 8.33 (d, J=3.51 Hz, 1H), 8.12 (br. s, 1H), 7.20-7.26 (m, 1H), 6.88 (s, 1H), 4.21 (q, J=6.78 Hz, 2H), 3.74-3.88 (m, 4H), 3.02-3.14 (br. s, 4H), 1.26 (t, J=6.90 Hz, 3H); LCMS (m/z): 385.1 [M+H]⁺.

Example 143 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{[2-methyl-4-(4-pyridinylmethoxy)phenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 141.

¹H NMR (DMSO-d₆) δ (ppm) 12.28 (br. s, 1H), 10.30 (br. s, 1H), 8.62 (d, J=4.40 Hz, 2H), 8.17 (d, J=4.40 Hz, 1H), 7.67-7.79 (m, 2H), 7.52 (d, J=4.89 Hz, 2H), 7.45 (d, J=8.31 Hz, 1H), 7.15 (br. s, 1H), 7.03 (d, J=8.31 Hz, 1H), 6.83 (s, 1H), 6.68-6.75 (m, 1H), 5.31 (s, 2H), 4.26 (q, J=7.17 Hz, 2H), 2.23 (s, 3H), 1.29 (t, J=6.85 Hz, 3H); LCMS (m/z): 496.8 [M+H]⁺.

Example 144 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-({4-[2-(diethylamino)ethoxy]-2-methylphenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate Acetate

The crude material, which similarly prepared according to the procedure described in the Example 141, was purified by preparative HPLC (aqueous ammonium acetate/acetonitrile) to afford the titled compound (solid).

¹H NMR (DMSO-d₆) δ (ppm) 12.17 (br. s, 1H), 8.16 (d, J=3.91 Hz, 1H), 7.75 (s, 1H), 7.68 (d, J=7.34 Hz, 1H), 7.34 (d, J=8.31 Hz, 1H), 7.00 (br. s, 1H), 6.91 (d, J=8.80 Hz, 1H), 6.70-6.79 (m, 2H), 4.19-4.30 (m, 2H), 4.10 (t, J=5.87 Hz, 2H), 2.86 (t, J=5.87 Hz, 2H), 2.61 (q, J=6.85 Hz, 4H), 2.20 (s, 3H), 1.90 (s, 3H), 1.28 (t, J=6.85 Hz, 3H), 1.02 (t, J=7.09 Hz, 6H); LCMS (m/z): 505.6 [M+H]⁺.

Example 145 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-({2-methyl-4-[2-(4-methylpiperazinyl)ethoxy]phenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate acetate

First Step

A solution of 4-nitro-m-cresol (2.0 g, 0.013 mol), 1-bromo-2-chloroethane (1.7 mL, 0.020 mol) and potassium carbonate (4.7 g, 0.034 mol) in N,N-dimethylformamide (21 mL) was stirred at 70° C. for 18 h. Cooled to ambient temperature, the reaction mixture was poured into ice water, extracted with ethyl acetate. The organic layer was washed with water and brine, dried over sodium sulfate and concentrated. The residue was purified by chromatography on silica gel(hexane/ethyl acetate) to afford 4-(2-chloroethoxy)-2-methyl-1-nitrobenzene as solid (2.1 g, y. 73%).

¹H NMR (DMSO-d₆) δ (ppm) 8.05 (d, J=9.12 Hz, 1H), 7.08 (d, J=2.48 Hz, 1H), 7.01 (dd, J=2.72, 9.08 Hz, 1H), 4.38 (t, J=5.04 Hz, 2H), 3.98 (t, J=5.08 Hz, 2H), 2.55 (s, 3H); LCMS (m/z): 216.2 [M+H]⁺.

Second Step

A solution of 4-(2-chloroethoxy)-2-methyl-1-nitrobenzene (2.1 g, 9.7 mmol), N-methylpiperazine (1.5 g, 15 mmol) and potassium carbonate (3.4 g, 0.025 mol) in N,N-dimethylformamide (15 mL) was stirred with heating at 70° C. for 18 h. Cooled to ambient temperature, the reaction mixture was poured into ice water, extracted with ethyl acetate. The organic layer was washed with water and brine, dried over sodium sulfate and concentrated. The residue was purified by chromatography on silica gel(dichloromethane/methanol) to afford 1-methyl-4-[2-(3-methyl-4-nitrophenoxy)ethyl]piperazine as solid (1.8 g, y. 64%).

¹H NMR (DMSO-d₆) δ (ppm) 8.03 (d, J=9.12 Hz, 1H), 7.05 (d, J=2.68 Hz, 1H), 6.98 (dd, J=2.76, 9.08 Hz, 1H), 4.18 (t, J=5.78 Hz, 2H), 2.69 (t, J=5.74 Hz, 2H), 2.54 (s, 3H), 2.39-2.52 (m, 4H), 2.22-2.37 (m, 4H), 2.14 (s, 3H); LCMS (m/z): 280.2 [M+H]⁺.

Third Step

1-Methyl-4-[2-(3-methyl-4-nitrophenoxy)ethyl]piperazine (0.69 g, 2.5 mmol) was dissolved in ethanol (10 mL) and 10% wt palladium on carbon (0.055 g) was added at ambient temperature. The reaction mixture was agitated under a hydrogen atmosphere at ambient temperature for 4 h. Palladium on carbon was removed by filtration with Celite and the solvent was removed under reduced pressure to afford 2-methyl-4-[2-(4-methylpiperazinyl)ethoxy]aniline as oil (0.60 g, y. 97%).

¹H NMR (DMSO-d₆) δ (ppm) 6.57 (br. s, 1H), 6.51 (br. s, 2H), 4.35 (br. s, 2H), 3.90 (t, J=5.90 Hz, 2H), 3.17 (br. s, 3H), 2.60 (t, J=5.88 Hz, 2H), 2.38-2.54 (m, 4H), 2.30 (br. s, 4H), 2.14 (s, 3H); LCMS (m/z): 250.4 [M+H]⁺.

Fourth Step

A solution of ethyl 2-ethoxy-4-oxo-4,5-dihydrofuran-3-carboxylate (0.32 g, 1.6 mmol) which afforded in the Example 74, Third step and 2-methyl-4-[2-(4-methylpiperazinyl)ethoxy]aniline (0.40 g, 1.6 mmol) in ethanol (5.0 mL) was stirred at 60° C. for 30 min. The solvent was removed under reduced pressure, then the residue was purified by chromatography on silica gel(dichloromethane/methanol) to afford 2-({2-methyl-4-[2-(4-methylpiperazinyl)ethoxy]phenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate as solid (0.30 g, y. 46%).

¹H NMR (DMSO-d₆) δ (ppm) 9.99 (br. s, 1H), 7.26 (d, J=8.56 Hz, 1H), 6.91 (d, J=2.28 Hz, 1H), 6.78-6.86 (m, 1H), 4.58 (s, 2H), 4.21 (q, J=7.04 Hz, 2H), 4.03-4.12 (m, 2H), 3.31 (s, 3H), 2.27-2.80 (m, 10H), 2.20 (s, 3H), 1.25 (t, J=7.04 Hz, 3H); LCMS (m/z): 404.2 [M+H]⁺.

Fifth Step

To a solution of 2-({2-methyl-4-[2-(4-methylpiperazinyl)ethoxy]phenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.22 g, 0.55 mmol) and 7-azaindole-3-carboxaldehyde (0.080 g, 0.55 mmol) in ethanol (14 mL), L-proline (0.014 g, 0.12 mmol) was added at ambient temperature. The mixture was refluxed for 2 days. The solvent was removed under reduced pressure, and then the residue was purified by preparative HPLC (aqueous ammonium acetate/acetonitrile) to afford the titled compound as solid. (0.032 g, y. 11%).

¹H NMR (DMSO-d₆) δ (ppm) 12.16 (br. s, 1H), 8.17 (d, J=4.40 Hz, 1H), 7.64-7.79 (m, 2H), 7.31 (d, J=8.31 Hz, 1H), 7.00 (br. s, 1H), 6.90 (d, J=8.31 Hz, 1H), 6.65-6.80 (m, 2H), 4.10-4.30 (m, 4H), 2.76 (t, J=5.62 Hz, 2H), 2.25-2.65 (m, 9H), 2.19-2.21 (m, 5H), 1.86 (s, 3H), 1.28 (t, J=6.85 Hz, 3H); LCMS (m/z): 532.3 [M+H]⁺.

Example 146 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{[4-(2-hydroxy-2-methylpropoxy)-2-methylphenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 141.

¹H (DMSO-d₆) δ (ppm) 12.28 (br. s, 1H), 10.30 (br. s, 1H), 8.16 (d, J=3.91 Hz, 1H), 7.80 (s, 1H), 7.60 (d, J=7.82 Hz, 1H), 7.40 (d, J=8.31 Hz, 1H), 7.03 (br. s, 1H), 6.94 (d, J=8.31 Hz, 1H), 6.81 (s, 1H), 6.73 (dd, J=4.40, 7.82 Hz, 1H), 4.72 (s, 1H), 4.27 (q, J=7.17 Hz, 2H), 3.81 (s, 2H), 2.22 (s, 3H), 1.25-1.33 (m, 9H); LCMS (m/z): 478.2 [M+H]⁺.

Example 147 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(2-methyl-4-{[2-(pyrrolidino)ethyl]amino}phenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate acetate

To a solution of 2-[(2-methyl-4-{[2-(pyrrolidino)ethyl]amino}phenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.32 g, 0.85 mmol) which similarly prepared according to the procedure described in the Example 125, First step to Third step and 7-azaindole-3-carboxaldehyde (0.13 g, 0.85 mmol) in ethanol (5.0 mL), piperidine (0.10 mL, 1.0 mmol) was added at ambient temperature. The mixture was refluxed for 24 h. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC (aqueous ammonium acetate/acetonitrile) to afford the titled compound as solid (0.010 g, y. 3%).

¹H NMR (DMSO-d₆) δ (ppm) 12.05 (br. s, 1H), 8.11-8.20 (m, 1H), 7.71-7.84 (m, 2H), 7.01-7.10 (m, 1H), 6.77-6.85 (m, 1H), 6.49-6.68 (m, 3H), 5.57 (br. s, 1H), 4.15-4.25 (m, 2H), 3.21 (br. s, 2H), 2.63-2.73 (m, 2H), 2.52-2.61 (m, 4H), 2.10 (s, 3H), 1.85 (s, 3H), 1.65-1.78 (m, 4H), 1.27 (t, J=6.85 Hz, 3H); LCMS (m/z): 502.4 [M+H]⁺.

Example 148 Isopropyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[N-methyl-N-(2-thienylmethyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

A solution of isopropyl 2-isopropoxy-4-oxo-4,5-dihydrofuran-3-carboxylate (0.10 g, 0.44 mmol) which similarly prepared according to the procedure described in the Example 74, Third step using diisopropyl malonate and chloroacetyl chloride, N-methyl-N-(2-thienylmethyl)amine (0.061 mg, 0.048 mmol) and triethylamine (0.18 mL, 1.3 mmol) in 2-propanol (2.0 mL) was stirred for 7 days then refluxed for further 24 h. To this reaction mixture, 7-azaindole-3-carboxaldehyde (0.065 g, 0.44 mmol) was added then the mixture was refluxed for 24 h. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC to afford the titled compound as solid (0.0038 g, y. 2%).

¹H NMR (DMSO-d₆) δ (ppm) 12.33 (br. s, 1H), 8.35 (d, J=8.03 Hz, 1H), 8.28-8.32 (m, 1H), 8.02 (s, 1H), 7.53 (dd, J=1.13, 5.14 Hz, 1H), 7.14-7.22 (m, 2H), 7.04 (dd, J=3.51, 5.02 Hz, 1H), 6.88 (s, 1H), 5.19 (s, 2H), 4.97-5.05 (m, 1H), 3.26 (s, 3H), 1.24 (d, J=6.27 Hz, 6H); LCMS (m/z): 424.1 [M+H]⁺.

Example 149 Isopropyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(cyclohexylamino)-4-oxo-4,5-dihydrofuran-3-carboxylate

A solution of isopropyl 2-isopropoxy-4-oxo-4,5-dihydrofuran-3-carboxylate (0.10 g, 0.44 mmol) which similarly prepared according to the procedure described in the Example 74, Third step using diisopropyl malonate and chloroacetyl chloride, and cyclohexylamine (0.055 mL, 0.48 mmol) in 2-propanol (2.0 mL) was stirred at ambient temperature for 14 h. To this reaction mixture, 7-azaindole-3-carboxaldehyde (0.065 g, 0.44 mmol) and piperidine (0.0044 mL, 0.044 mmol) were added then the mixture was refluxed for further 8 days. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC to afford the titled compound as solid (0.0079 g, y. 4%).

¹H NMR (DMSO-d₆) δ (ppm) 12.35 (br. s, 1H), 8.51 (d, J=8.28 Hz, 1H), 8.41 (d, J=7.78 Hz, 1H), 8.32 (dd, J=1.38, 4.64 Hz, 1H), 7.99 (d, J=2.51 Hz, 1H), 7.20 (dd, J=4.64, 7.91 Hz, 1H), 6.86 (s, 1H), 5.00-5.12 (m, 1H), 3.96-4.05 (m, 1H), 1.93-2.04 (m, 2H), 1.72-1.84 (m, 2H), 1.54-1.69 (m, 3H), 1.35-1.49 (m, 2H), 1.18-1.31 (m, 7H); LCMS (m/z): 396.2 [M+H]⁺.

Example 150 Isopropyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(cyclohexylmethyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

A solution of isopropyl 2-isopropoxy-4-oxo-4,5-dihydrofuran-3-carboxylate (0.10 g, 0.44 mmol) which similarly prepared according to the procedure described in the Example 74, Third step using diisopropyl malonate and chloroacetyl chloride, and aminomethylcyclohexane (0.063 mL, 0.48 mmol) in 2-propanol (2.0 mL) was stirred at ambient temperature for 14 h. To this reaction mixture, 7-azaindole-3-carboxaldehyde (0.065 g, 0.44 mmol) and piperidine (0.0044 mL, 0.044 mmol) were added then the mixture was refluxed for further 8 days. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC to afford the titled compound as solid (0.036 g, y. 19%).

¹H NMR (DMSO-d₆) δ (ppm) 12.35 (br. s, 1H), 8.90-8.98 (m, 1H), 8.41 (d, J=6.78 Hz, 1H), 8.32 (dd, J=1.38, 4.64 Hz, 1H), 7.99 (s, 1H), 7.19 (dd, J=4.77, 8.03 Hz, 1H), 6.84 (s, 1H), 5.00-5.13 (m, 1H), 3.45-3.53 (m, 2H), 1.56-1.81 (m, 5H), 1.27 (d, J=6.27 Hz, 6H), 1.08-1.24 (m, 4H), 0.94-1.07 (m, 2H); LCMS (m/z): 410.2 [M+H]⁺.

Example 151 Isopropyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(trans-4-hydroxycyclohexyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

A solution of isopropyl 2-[(trans-4-hydroxycyclohexyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.10 g, 0.44 mmol) which similarly prepared according to the procedure described in the Example 74, Third step using diisopropyl malonate and chloroacetyl chloride, and trans-4-aminomethylcyclohexanol (0.056 mg, 0.49 mmol) in 2-propanol (1.0 mL) was stirred at ambient temperature for 20 h. To this reaction mixture, 7-azaindole-3-carboxaldehyde (0.065 g, 0.44 mmol) and L-proline (0.0050 g, 0.044 mmol) were added then the mixture was refluxed for further 1.5 days. The precipitate was collected by filtration, washed with hot ethanol. The solid was washed with hexane then dried to afford isopropyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(trans-4-hydroxycyclohexyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate as solid (0.0076 g, y. 4%).

¹H NMR (DMSO-d₆) δ (ppm) 12.36 (br. s, 1H), 8.46 (d, J=8.28 Hz, 1H), 8.42 (d, J=7.53 Hz, 1H), 8.33 (dd, J=1.38, 4.64 Hz, 1H), 7.99 (d, J=2.51 Hz, 1H), 7.20 (dd, J=4.64, 7.91 Hz, 1H), 6.85 (s, 1H), 5.01-5.09 (m, 1H), 4.69 (d, J=4.27 Hz, 1H), 3.93-3.99 (m, 1H), 3.43-3.51 (m, 1H), 1.86-2.03 (m, 4H), 1.60-1.73 (m, 2H), 1.29-1.42 (m, 2H), 1.26 (d, J=6.27 Hz, 6H); LCMS (m/z): 412.1 [M+H]⁺.

Example 152 Cyclopropylmethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-({4-[2-(dimethylamino)ethoxy]-2-methylphenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate formate

A solution of ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-({4-[2-(dimethylamino)ethoxy]-2-methylphenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.033 g, 0.070 mmol) which similarly prepared according to the procedure described in the Example 111, cyclopropyl carbinol (0.10 mL, 1.3 mmol) and zinc cluster catalyst (Zn₄(OCOCF₃)₆O) (0.0033 g, 0.0035 mmol) in N,N-dimethylacetamide (0.9 mL) was stirred with the microwave synthesizer (Biotage Initiator™) at 150° C. for 30 min. Cooled to ambient temperature, the precipitate was removed by filtration. The filtrate was purified by preparative HPLC to afford the titled compound as solid (0.0029 g, y. 8%).

¹H NMR (DMSO-d₆) δ (ppm) 12.25 (br. s, 1H), 8.04-8.29 (m, 2H), 7.75 (s, 1H), 7.68 (d, J=7.78 Hz, 1H), 7.38 (d, J=8.28 Hz, 1H), 7.03 (d, J=2.51 Hz, 1H), 6.93 (d, J=8.78 Hz, 1H), 6.70-6.83 (m, 2H), 6.55 (br. s, 1H), 4.15 (t, J=5.65 Hz, 2H), 4.06 (d, J=6.78 Hz, 2H), 2.70 (t, J=5.80 Hz, 2H), 2.28 (s, 6H), 2.22 (s, 3H), 1.15-1.32 (m, 1H), 0.49-0.62 (m, 2H), 0.32-0.39 (m, 2H); LCMS (m/z): 503.2 [M+H]⁺.

Example 153 Cyclopropylmethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{[4-(2-hydroxyethoxy)-2-methylphenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate

A solution of the compound (0.032 g, 0.070 mmol) of Example 74, cyclopropyl carbinol (0.10 mL, 1.3 mmol) and zinc cluster catalyst (Zn₄(OCOCF₃)₆O) (0.0033 g, 0.0035 mmol) in N,N-dimethylacetamide (0.9 mL) was stirred with the microwave synthesizer (Biotage Initiator™) at 150° C. for 30 min. Cooled to ambient temperature, the precipitate was removed by filtration. The filtrate was purified by preparative HPLC to afford the titled compound as solid (0.0040 g, y. 11%).

¹H NMR (DMSO-d₆) δ (ppm) 12.23 (br. s, 1H), 10.36 (br. s, 1H), 8.19 (d, J=3.51 Hz, 1H), 7.65-7.80 (m, 2H), 7.38 (d, J=8.53 Hz, 1H), 7.01 (d, J=2.26 Hz, 1H), 6.92 (dd, J=2.51, 8.53 Hz, 1H), 6.72-6.84 (m, 2H), 4.95 (t, J=5.40 Hz, 1H), 4.01-4.12 (m, 4H), 3.79 (q, J=5.02 Hz, 2H), 2.22 (s, 3H), 1.14-1.26 (m, 1H), 0.51-0.58 (m, 2H), 0.31-0.39 (m, 2H); LCMS (m/z): 476.1 [M+H]⁺.

Example 154 Isopropyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-({4-[2-(dimethylamino)ethoxy]-2-methylphenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate formate

A solution of ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-({4-[2-(dimethylamino)ethoxy]-2-methylphenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.033 g, 0.070 mmol) which similarly prepared according to the procedure described in the Example 111, and zinc cluster catalyst (Zn₄(OCOCF₃)₆O) (0.0033 g, 0.0035 mmol) in 2-propanol (0.5 mL) and N,N-dimethylacetamide (0.5 mL) was stirred with the microwave synthesizer (Biotage Initiator™) at 150° C. for 30 min. Cooled to ambient temperature, the precipitate was removed by filtration. The filtrate was purified by preparative HPLC to afford the titled compound as solid (0.0046 g, y. 11%).

¹H NMR (DMSO-d₆) δ (ppm) 12.28 (br. s, 1H), 10.20 (br. s, 1H), 8.18 (dd, J=1.60, 4.40 Hz, 1H), 8.14 (s, 1H), 7.78 (s, 1H), 7.67 (d, J=8.03 Hz, 1H), 7.42 (d, J=8.53 Hz, 1H), 7.04 (d, J=2.51 Hz, 1H), 6.94 (dd, J=2.76, 8.78 Hz, 1H), 6.72-6.81 (m, 2H), 5.10-5.17 (m, 1H), 4.16 (t, J=5.65 Hz, 2H), 2.75 (t, J=5.52 Hz, 2H), 2.31 (s, 6H), 2.23 (s, 3H), 1.31 (d, J=6.27 Hz, 6H); LCMS (m/z): 491.2 [M+H]⁺.

Example 155 n-Butyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-({4-[2-(dimethylamino)ethoxy]-2-methylphenyl}amino)-4-oxo-4,5-dihydrofuran-3carboxylate formate

A solution of ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-({4-[2-(dimethylamino)ethoxy]-2-methylphenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.033 g, 0.070 mmol) which similarly prepared according to the procedure described in the Example 111, and zinc cluster catalyst (Zn₄(OCOCF₃)₆O) (0.0033 g, 0.0035 mmol) in n-butanol (0.5 mL) and N,N-dimethylacetamide (0.5 mL) was stirred with the microwave synthesizer (Biotage Initiator™) at 150° C. for 30 min. Cooled to ambient temperature, the precipitate was removed by filtration. The filtrate was purified by preparative HPLC to afford the titled compound as solid (0.011 g, y. 31%).

¹H NMR (DMSO-d₆) δ (ppm) 12.28 (br. s, 1H), 10.32 (br. s, 1H), 8.18 (dd, J=1.38, 4.64 Hz, 1H), 8.15 (s, 1H), 7.76 (s, 1H), 7.67 (d, J=7.78 Hz, 1H), 7.41 (d, J=8.78 Hz, 1H), 7.04 (d, J=2.51 Hz, 1H), 6.94 (dd, J=2.76, 8.78 Hz, 1H), 6.81 (s, 1H), 6.75 (dd, J=4.77, 8.03 Hz, 1H), 4.22 (t, J=6.78 Hz, 2H), 4.16 (t, J=5.65 Hz, 2H), 2.74 (t, J=5.65 Hz, 2H), 2.30 (s, 6H), 2.22 (s, 3H), 1.60-1.72 (m, 2H), 1.34-1.51 (m, 2H), 0.94 (t, J=7.40 Hz, 3H); LCMS (m/z): 505.2 [M+H]⁺.

Example 156 n-Butyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{[4-(2-hydroxyethoxy)-2-methylphenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate

A solution of the compound (0.032 g, 0.070 mmol) of Example 74, and zinc cluster catalyst (Zn₄(OCOCF₃)₆O) (0.0033 g, 0.0035 mmol) in n-butanol (0.5 mL) and N,N-dimethylacetamide (0.5 mL) was stirred with the microwave synthesizer (Biotage Initiator™) at 150° C. for 30 min. Cooled to ambient temperature, the precipitate was removed by filtration. The filtrate was purified by preparative HPLC to afford the titled compound as solid (0.015 g, y. 44%).

¹H NMR (DMSO-d₆) δ (ppm) 12.27 (br. s, 1H), 10.27 (s, 1H), 8.19 (dd, J=1.13, 4.64 Hz, 1H), 7.74 (d, J=2.26 Hz, 1H), 7.69 (d, J=7.78 Hz, 1H), 7.42 (d, J=8.78 Hz, 1H), 7.03 (d, J=2.76 Hz, 1H), 6.94 (dd, J=2.64, 8.66 Hz, 1H), 6.74-6.85 (m, 2H), 4.88-5.05 (m, 1H), 4.22 (t, J=6.65 Hz, 2H), 4.09 (t, J=4.89 Hz, 2H), 3.75-3.85 (m, 2H), 2.23 (s, 3H), 1.59-1.73 (m, 2H), 1.36-1.49 (m, 2H), 0.94 (t, J=7.40 Hz, 3H); LCMS (m/z): 478.2 [M+H]⁺.

Example 157 Isopropyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{[4-(2-hydroxyethoxy)-2-methylphenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate

A solution of the compound (0.032 g, 0.070 mmol) of Example 74, and zinc cluster catalyst (Zn₄(OCOCF₃)₆O) (0.0033 g, 0.0035 mmol) in 2-propanol (0.5 mL) and N,N-dimethylacetamide (0.5 mL) was stirred with the microwave synthesizer (Biotage Initiator™) at 150° C. for 30 min. Cooled to ambient temperature, the precipitate was removed by filtration. The filtrate was purified by preparative HPLC to afford the titled compound as solid (0.010 g, y. 28%).

¹H NMR (DMSO-d₆) δ (ppm) 12.27 (br. s, 1H), 10.22 (s, 1H), 8.19 (dd, J=1.26, 4.77 Hz, 1H), 7.76 (d, J=2.51 Hz, 1H), 7.69 (d, J=7.78 Hz, 1H), 7.42 (d, J=8.53 Hz, 1H), 7.03 (d, J=2.51 Hz, 1H), 6.94 (dd, J=2.76, 8.78 Hz, 1H), 6.73-6.84 (m, 2H), 5.08-5.18 (m, 1H), 4.95 (t, J=5.40 Hz, 1H), 4.09 (t, J=4.89 Hz, 2H), 3.80 (q, J=5.27 Hz, 2H), 2.23 (s, 3H), 1.31 (d, J=6.27 Hz, 6H); LCMS (m/z): 464.1 [M+H]⁺.

Example 158 Isopropyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(cyclopropylamino)-4-oxo-4,5-dihydrofuran-3-carboxylate

A solution of isopropyl 2-isopropoxy-4-oxo-4,5-dihydrofuran-3-carboxylate (0.10 g, 0.44 mmol) which similarly prepared according to the procedure described in the Example 74, Third step using diisopropyl malonate and chloroacetyl chloride, and cyclopropylamine (0.034 mL, 0.48 mmol) in 2-propanol (2.0 mL) was stirred at ambient temperature for 14 h. To this reaction mixture, 7-azaindole-3-carboxaldehyde (0.065 g, 0.44 mmol) and piperidine (0.0044 mL, 0.044 mmol) were added then the mixture was refluxed for further 12 days. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC to afford the titled compound as solid (0.0020 g, y. 1%).

¹H NMR (DMSO-d₆) δ (ppm) 12.33 (br. s, 1H), 8.81 (br. s, 1H), 8.56 (d, J=7.28 Hz, 1H), 8.31 (d, J=3.51 Hz, 1H), 8.06 (d, J=2.26 Hz, 1H), 7.21 (dd, J=4.64, 7.91 Hz, 1H), 6.88 (s, 1H), 4.99-5.10 (m, 1H), 3.09-3.18 (m, 1H), 1.26 (d, J=6.27 Hz, 6H), 0.89-0.97 (m, 4H); LCMS (m/z): 354.1 [M+H]⁺.

Example 159 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(cyclohexylmethyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

A solution of ethyl 2-ethoxy-4-oxo-4,5-dihydrofuran-3-carboxylate (0.10 g, 0.51 mmol) which afforded in the Example 74, Third step and aminomethylcyclohexane (0.072 mL, 0.55 mmol) in ethanol (2.0 mL) was stirred at ambient temperature for 1.5 h. To this reaction mixture, 7-azaindole-3-carboxaldehyde (0.073 g, 0.50 mmol) and L-proline (0.012 mg, 0.10 mmol) were added then the mixture was refluxed for further 5 days. The precipitate was collected by filtration, washed with hot ethanol. The solid was washed with hexane then dried to afford the titled compound as solid (0.024 g, y. 12%).

¹H NMR (DMSO-d₆) δ (ppm) 12.35 (br. s, 1H), 8.98 (t, J=6.27 Hz, 1H), 8.42 (d, J=7.78 Hz, 1H), 8.32 (dd, J=1.38, 4.64 Hz, 1H), 7.99 (d, J=2.51 Hz, 1H), 7.19 (dd, J=4.64, 7.91 Hz, 1H), 6.88 (s, 1H), 4.21 (q, J=7.03 Hz, 2H), 3.48 (t, J=6.40 Hz, 2H), 1.65-1.81 (m, 5H), 1.62 (d, J=9.29 Hz, 1H), 1.09-1.30 (m, 6H), 0.95-1.06 (m, 2H); LCMS (m/z): 396.2 [M+H]⁺.

Example 160 2-Hydroxyethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[N-methyl-N-(2-thienylmethyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

A solution of the compound (0.050 g, 0.12 mmol) of Example 108, ethylene glycol (0.20 mL, 3.6 mmol), 4-dimethylaminopyridine (0.0030 g, 0.024 mmol) and zinc cluster catalyst (Zn₄(OCOCF₃)₆O) (0.0058 g, 0.0061 mmol) in N,N-dimethylacetamide (0.5 mL) was stirred with the microwave synthesizer (Biotage Initiator™) at 150° C. for 30 min. Cooled to ambient temperature, the precipitate was removed by filtration. The filtrate was purified by preparative HPLC to afford the titled compound as solid (0.0061 g, y. 10%).

¹H NMR (DMSO-d₆) δ (ppm) 12.34 (br. s, 1H), 11.7 (br. s, 1H), 8.35-8.44 (m, 1H), 8.30 (dd, J=1.38, 4.64 Hz, 1H), 8.04 (s, 1H), 7.53 (dd, J=1.13, 5.14 Hz, 1H), 7.21 (d, J=3.01 Hz, 1H), 7.17 (dd, J=4.64, 7.91 Hz, 1H), 7.04 (dd, J=3.51, 5.02 Hz, 1H), 6.94 (s, 1H), 5.20 (s, 2H), 4.17 (t, J=5.14 Hz, 2H), 3.62 (q, J=5.52 Hz, 2H), 3.23-3.37 (m, 3H); LCMS (m/z): 426.1 [M+H]⁺.

Example 161 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{[2-methyl-4-(2-pyrimidinylmethoxy)phenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate

First Step

A solution of 4-nitro-m-cresol (0.25 g, 1.6 mmol), 2-(chloromethyl)pyrimidine hydrochloride (0.40 g, 2.4 mmol) and potassium carbonate (0.50 g, 4.0 mmol) in N,N-dimethylformamide (6.0 mL) was stirred at 70° C. for 18 h. Cooled to ambient temperature, the reaction mixture was poured into ice water, extracted with ethyl acetate. The organic layer was washed with water and brine, dried over sodium sulfate, filtered and concentrated to afford 2-[(3-methyl-4-nitrophenoxy)methyl]pyrimidine as crude material (0.32 g, y. 81%) as solid.

¹H NMR (DMSO-d₆) δ (ppm) 8.84 (d, J=4.92 Hz, 2H), 8.03 (d, J=9.08 Hz, 1H), 7.48 (t, J=4.90 Hz, 1H), 7.10 (d, J=2.68 Hz, 1H), 7.00 (dd, J=2.80, 9.12 Hz, 1H), 5.43 (s, 2H), 2.53 (s, 3H); LCMS (m/z): 246.4 [M+H]⁺.

Second Step

To a solution of 2-[(3-methyl-4-nitrophenoxy)methyl]pyrimidine (0.18 g, 0.7 mmol) in ethanol (4.0 mL) and water (1.0 mL), iron (0.40 g, 7.2 mmol) and ammonium chloride (0.020 g, 0.37 mmol) were added. The mixture was refluxed for 1.5 h. Cooled to ambient temperature, the reaction mixture was filtered over Celite to remove unneeded materials and the filtrate was concentrated to afford 2-methyl-4-(2-pyrimidinylmethoxy)aniline (0.15 g, crude material) as solid.

¹H NMR (DMSO-d₆) δ (ppm) 8.82 (d, J=4.88 Hz, 2H), 7.45 (t, J=4.90 Hz, 1H), 6.62 (d, J=2.64 Hz, 1H), 6.55 (dd, J=2.74, 8.60 Hz, 1H), 6.50 (d, J=8.52 Hz, 1H), 5.08 (s, 2H), 4.40 (br. s, 2H), 2.01 (s, 3H); LCMS (m/z): 216.0 [M+H]⁺.

Third Step

A solution of ethyl 2-ethoxy-4-oxo-4,5-dihydrofuran-3-carboxylate (0.14 g, 0.60 mmol) which afforded in the Example 74, Third step and 2-methyl-4-(2-pyrimidinylmethoxy)aniline (0.13 g, 0.6 mmol) in ethanol (5.0 mL) was stirred at 60° C. for 30 h. The solvent was removed under reduced pressure, then the residue was purified by chromatography on silica gel(hexane/ethyl acetate) to afford ethyl 2-{[2-methyl-4-(2-pyrimidinylmethoxy)phenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate as solid (0.035 g, y. 15%).

¹H NMR (DMSO-d₆) δ (ppm) 9.98 (br. s, 1H), 8.84 (d, J=4.88 Hz, 2H), 7.47 (t, J=4.90 Hz, 1H), 7.25 (d, J=8.76 Hz, 1H), 6.96 (d, J=2.72 Hz, 1H), 6.84 (dd, J=2.82, 8.70 Hz, 1H), 5.28 (s, 2H), 4.57 (s, 2H), 4.21 (q, J=7.07 Hz, 2H), 2.19 (s, 3H), 1.24 (t, J=7.04 Hz, 3H); LCMS (m/z): 370.0 [M+H]⁺.

Fourth Step

To a solution of ethyl 2-{[2-methyl-4-(2-pyrimidinylmethoxy)phenyl]amino}-4-oxo-4,5-dihydrofuran-3-carboxylate (0.035 g, 0.10 mmol) and 7-azaindole-3-carboxaldehyde (0.014 g, 0.10 mmol) in ethanol (5.0 mL), piperidine (0.050 mL, 0.50 mmol) was added at ambient temperature then the mixture was refluxed for 3 days. Cooled to ambient temperature, the solvent was removed under reduced pressure. The residue was suspended in ethanol, then the precipitate was collected by filtration, washed with ethanol then dried to afford the titled compound as solid (9.0 mg, y. 19%).

¹H NMR (DMSO-d₆) δ (ppm) 12.28 (br. s, 1H), 10.27 (br. s, 1H), 8.88 (d, J=4.40 Hz, 2H), 8.19 (d, J=3.42 Hz, 1H), 7.77 (br. s, 1H), 7.68 (d, J=7.34 Hz, 1H), 7.50 (t, J=4.60 Hz, 1H), 7.42 (d, J=8.80 Hz, 1H), 7.10 (br. s, 1H), 6.98 (d, J=8.31 Hz, 1H), 6.74-6.86 (m, 2H), 5.39 (s, 2H), 4.19-4.32 (m, J=6.80 Hz, 2H), 2.22 (br. s, 3H), 1.18-1.47 (m, 3H); LCMS (m/z): 498.4 [M+H]⁺.

Example 162 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-({2-methyl-4-[2-(1-pyrrolidinyl)ethoxy]phenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate acetate

The mixture, which similarly prepared according to the procedure described in the Example 161, was purified by preparative HPLC (aqueous ammonium acetate/acetonitrile were used as eluents) to afford the titled compound (solid).

¹H NMR (DMSO-d₆) δ (ppm) 12.25 (br. s, 1H), 8.16 (d, J=3.91 Hz, 1H), 7.74 (s, 1H), 7.68 (d, J=7.82 Hz, 1H), 7.35 (d, J=7.82 Hz, 1H), 7.02 (br. s, 1H), 6.86-6.96 (m, 2H), 6.70-6.80 (m, 1H), 4.20-4.35 (m, 2H), 4.16 (t, J=5.62 Hz, 2H), 2.87 (t, J=5.62 Hz, 2H), 2.50-2.72 (m, 4H), 2.21 (s, 3H), 1.90 (s, 3H), 1.68-1.78 (m, 4H), 1.28 (t, J=7.09 Hz, 3H); LCMS (m/z): 503.2 [M+H]⁺.

Example 163 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-({2-methyl-4-[2-(methylsulfonyl)ethoxy]phenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate

To a mixed solution of the compound (0.070 g, 0.14 mmol) of Example 128 and saturated sodium bicarbonate solution (0.80 mL) in methanol (0.4 mL) and dichloromethane (1.6 mL) that cooled with ice bath, 3-chloroperoxybenzoic acid (0.050 g, 0.28 mmol) was added at 0° C. The mixture was stirred for 1 h. The reaction mixture was extracted with dichloromethane, and the organic layer was washed with saturated sodium bicarbonate solution, dried over sodium sulfate and concentrated. The residue was purified by preparative HPLC to afford the titled compound as solid (0.010 g, y. 13%).

¹H NMR (DMSO-d₆) δ (ppm) 12.18 (br. s, 1H), 10.4 (br. s, 1H), 8.19 (d, J=4.40 Hz, 1H), 7.74 (br. s, 1H), 7.64 (d, J=7.34 Hz, 1H), 7.36 (d, J=8.31 Hz, 1H), 7.06 (br. s, 1H), 6.97 (d, J=8.31 Hz, 1H), 6.66-6.80 (m, 2H), 4.44 (t, J=5.38 Hz, 2H), 4.18-4.30 (m, J=6.80 Hz, 2H), 3.66-3.76 (m, 2H), 3.15 (s, 3H), 2.21 (s, 3H), 1.28 (t, J=7.09 Hz, 3H); LCMS (m/z): 512.2 [M+H]⁺.

Example 164 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(7,8-dihydro-1,6-naphthyridin-6(5H)-yl)-4-oxo-4,5-dihydrofuran-3-carboxylate formate

To a solution of 5,6,7,8-tetrahydro-1,6-naphthyridine dihydrochloride (0.049 g, 0.24 mmol) in ethanol (1.0 mL), 4M sodium hydroxide solution (0.13 mL, 0.52 mmol) was added at ambient temperature and the mixture was stirred for 5 min. To this reaction mixture, ethyl 2-ethoxy-4-oxo-4,5-dihydrofuran-3-carboxylate (0.049 g, 0.24 mmol), which afforded in the Example 74, Third step was added and stirred for 1 h. The solvent was removed under reduced pressure, then chloroform and water were added to the residue and two phases were separated. The organic layer was washed with brine, dried over magnesium sulfate and concentrated. To this residue, 7-azaindole-3-carboxaldehyde (0.027 g, 0.18 mmol), 5,6,7,8-tetrahydro-1,6-naphthyridine (0.0063 g, 0.047 mmol) and ethanol (1.0 mL) were added and the mixture was refluxed for further 14 days. The reaction mixture was filtered and washed with hot ethanol. The solid was purified by preparative HPLC to afford the titled compound as solid (0.0058 g, y. 5%).

¹H NMR (DMSO-d₆) δ (ppm) 12.38 (br. s, 1H), 8.46 (d, J=3.51 Hz, 1H), 8.42 (d, J=7.78 Hz, 1H), 8.33 (dd, J=1.13, 4.64 Hz, 1H), 8.10 (s, 1H), 7.71 (d, J=7.78 Hz, 1H), 7.31 (dd, J=4.77, 7.78 Hz, 1H), 7.22 (dd, J=4.64, 7.91 Hz, 1H), 6.91 (s, 1H), 5.01-5.07 (m, 2H), 4.22 (q, J=7.03 Hz, 2H), 4.07-4.13 (m, 2H), 3.19 (t, J=5.90 Hz, 2H), 1.27 (t, J=7.03 Hz, 3H); LCMS (m/z): 417.1 [M+H]⁺.

Example 165 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(5,6-dihydro-1,7-naphthyridin-7(8H)-yl)-4-oxo-4,5-dihydrofuran-3-carboxylate formate

To a solution of 5,6,7,8-tetrahydro-1,7-naphthyridine dihydrochloride (0.050 g, 0.24 mmol) in ethanol (1.3 mL), 4M sodium hydroxide solution (0.13 mL, 0.52 mmol) was added at ambient temperature and the mixture was stirred for 5 min. To this reaction mixture, ethyl 2-ethoxy-4-oxo-4,5-dihydrofuran-3-carboxylate (0.049 g, 0.24 mmol), which afforded in the Example 74, Third step was added and stirred for 1 h. The solvent was removed under reduced pressure, then chloroform and water were added to the residue and two phases were separated. The organic layer was washed with brine, dried over magnesium sulfate and concentrated. To this residue, 7-azaindole-3-carboxaldehyde (0.023 g, 0.15 mmol), 5,6,7,8-tetrahydro-1,7-naphthyridine dihydrochloride (0.0056 g, 0.042 mmol) and ethanol (1.0 mL) were added and refluxed for further 14 days. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC to afford the titled compound as solid (0.0033 g, y. 3%).

¹H NMR (DMSO-d₆) δ (ppm) 12.39 (br. s, 1H), 8.39-8.49 (m, 2H), 8.33 (d, J=4.52 Hz, 1H), 8.05 (d, J=2.26 Hz, 1H), 7.70 (d, J=7.03 Hz, 1H), 7.31 (dd, J=4.77, 7.78 Hz, 1H), 7.21 (dd, J=4.77, 7.78 Hz, 1H), 6.92 (s, 1H), 5.00 (br. s, 2H), 4.22 (q, J=7.03 Hz, 2H), 4.02-4.10 (m, 2H), 3.12 (t, J=5.52 Hz, 2H), 1.27 (t, J=7.03 Hz, 3H); LCMS (m/z): 417.1 [M+H]⁺.

Example 166 2-[(tert-Butoxycarbonyl)oxy]ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-azepinyl-4-oxo-4,5-dihydrofuran-3-carboxylate

First Step

To a solution of triethylamine (0.65 mL, 4.7 mmol) in ethylene glycol (1.8 mL, 0.031 mol) that cooled with ice bath, ethyl malonyl chloride (0.40 mL, 3.1 mmol) was added dropwise and the mixture was stirred for 2.5 h. The reaction was terminated by addition of aqueous 10% citric acid solution, the reaction mixture was extracted with chloroform. The organic layer was dried over magnesium sulfate, filtered and concentrated. The residue was purified by chromatography on silica gel(chloroform/methanol) to afford ethyl (2-hydroxyethyl)malonate as oil (0.37 g, y. 66%).

¹H NMR (CDCl₃) δ (ppm) 4.28-4.35 (m, 2H), 4.22 (q, J=7.19 Hz, 2H), 3.80-3.88 (m, 2H), 3.43 (s, 2H), 2.18 (br. s, 1H), 1.29 (t, J=7.15 Hz, 3H)

Second Step

To a solution of ethyl 2-hydroxyethyl malonate (0.36 mg, 2.1 mmol) and di-tert-butyl dicarbonate (0.57 mL, 2.5 mmol) in tetrahydrofuran (2.0 mL) that cooled with ice bath, triethylamine (0.43 mL, 3.1 mmol) was added dropwise and 4-dimethylaminopyridine (0.019 mg, 0.16 mmol) was added then the mixture was stirred at ambient temperature for 2 weeks. The reaction mixture was diluted with water, and extracted with ethyl acetate. The organic layer was washed with brine, dried over magnesium sulfate and concentrated. The residue was purified by chromatography on silica gel(hexane/ethyl acetate) to afford 2-[(tert-butoxycarbonyl)oxy]ethyl ethyl malonate as oil (0.37 g, y. 43%).

¹H NMR (CDCl₃) δ (ppm) 4.33-4.39 (m, 2H), 4.26-4.31 (m, 2H), 4.21 (q, J=7.03 Hz, 2H), 3.41 (s, 2H), 1.49 (s, 9H), 1.28 (t, J=7.15 Hz, 3H)

Third Step

A solution of 2-[(tert-butoxycarbonyl)oxy]ethyl ethyl malonate (0.36 g, 1.3 mmol) in anhydrous tetrahydrofuran (1.0 mL) was added dropwise to a solution of sodium hydride (60% w/w in oil, 0.10 g, 2.6 mmol) in anhydrous tetrahydrofuran (4.0 mL) that cooled with ice bath. The mixture was refluxed for 14 min. The reaction mixture was cooled with ice bath, chloroacetyl chloride (0.11 mL, 1.3 mmol) was added dropwise to the reaction mixture and the mixture was stirred for 1 h then stirred at 45° C. for further 1 h. The reaction mixture was diluted with water, extracted with chloroform for 2 times. The organic layer was dried over magnesium sulfate and concentrated. The residue was purified by chromatography on silica gel(ethyl acetate/methanol) then concentrated. To this residue (ca. 0.11 g) in ethanol (3.0 mL), hexamethyleneimine (0.046 mL, 0.41 mmol) was added dropwise and the mixture was stirred at ambient temperature for 30 min. Then to the reaction mixture, 7-azaindole-3-carboxaldehyde (0.043 g, 0.30 mmol) was added at ambient temperature then the mixture was refluxed for further 4 days. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC to afford the titled compound as solid (0.020 g, y. 3%).

¹H NMR (DMSO-d₆) δ (ppm) 12.31 (br. s, 1H), 8.39 (d, J=8.03 Hz, 1H), 8.31 (dd, J=1.38, 4.64 Hz, 1H), 7.95 (d, J=2.51 Hz, 1H), 7.20 (dd, J=4.64, 7.91 Hz, 1H), 6.85 (s, 1H), 4.30-4.36 (m, 2H), 4.21-4.27 (m, 2H), 3.70-3.90 (m, 4H), 1.80-1.90 (m, 4H), 1.53-1.62 (m, 4H), 1.42 (s, 9H); LCMS (m/z): 498.2 [M+H]⁺.

Example 167 2-Methoxyethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[N-methyl-N-(2-thienylmethyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

A solution of the compound (0.050 g, 0.12 mmol) of Example 108, 2-methoxyethanol (0.20 mL, 2.6 mmol), 4-dimethylaminopyridine (0.0030 g, 0.024 mmol) and zinc cluster catalyst (Zn₄(OCOCF₃)₆O) (0.0058 g, 0.0061 mmol) in N,N-dimethylacetamide (0.5 mL) was stirred with the microwave synthesizer (Biotage Initiator™) at 150° C. for 3.5 h. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC to afford the titled compound as solid (0.0029 g, y. 5%).

¹H NMR (DMSO-d₆) δ (ppm) 12.34 (br. s, 1H), 8.38 (d, J=8.03 Hz, 1H), 8.30 (dd, J=1.38, 4.64 Hz, 1H), 8.03 (d, J=2.26 Hz, 1H), 7.53 (dd, J=1.00, 5.02 Hz, 1H), 7.13-7.22 (m, 2H), 7.04 (dd, J=3.51, 5.02 Hz, 1H), 6.93 (s, 1H), 5.20 (s, 2H), 4.23-4.28 (m, 2H), 3.55-3.60 (m, 2H), 3.22-3.32 (m, 6H); LCMS (m/z): 440.1 [M+H]⁺.

Example 168 n-Butyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[N-methyl-N-(2-thienylmethyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

A solution of the compound (0.050 g, 0.12 mmol) of Example 108, 1-butanol (0.50 mL, 5.5 mmol), 4-dimethylaminopyridine (0.0030 g, 0.024 mmol) and zinc cluster catalyst (Zn₄(OCOCF₃)₆O) (0.0058 g, 0.0061 mmol) in N,N-dimethylacetamide (0.5 mL) was stirred with the microwave synthesizer (Biotage Initiator™) at 150° C. for 1.5 h. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC to afford the titled compound as solid (0.0044 g, y. 6%).

¹H NMR (DMSO-d₆) δ (ppm) 12.33 (br. s, 1H), 8.37 (d, J=7.78 Hz, 1H), 8.30 (dd, J=1.25, 4.52 Hz, 1H), 8.01-8.04 (m, 1H), 7.53 (dd, J=1.25, 5.02 Hz, 1H), 7.14-7.22 (m, 2H), 7.04 (dd, J=3.51, 5.02 Hz, 1H), 6.91 (s, 1H), 5.21 (s, 2H), 4.13 (t, J=6.53 Hz, 2H), 3.24-3.31 (m, 3H), 1.55-1.65 (m, 2H), 1.36-1.47 (m, 2H), 0.90 (t, J=7.40 Hz, 3H); LCMS (m/z): 438.1 [M+H]⁺.

Example 169 2-Hydroxyethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-azepinyl-4-oxo-4,5-dihydrofuran-3-carboxylate hydrochloride

To a mixed solution of the compound (0.018 g, 0.037 mmol) of Example 166 in dioxane (0.2 mL) and chloroform (1.0 mL) that cooled with ice bath, 4M hydrochloric acid in dioxane (0.25 mL, 1.0 mmol) was added dropwise. The mixture was stirred at ambient temperature for 24 h. The precipitate was collected by filtration, washed with chloroform and hexane then dried to afford the titled compound as solid (6.3 mg, y. 37%).

¹H NMR (DMSO-d₆) δ (ppm) 12.39 (br. s, 1H), 8.44 (d, J=7.78 Hz, 1H), 8.33 (dd, J=1.38, 4.64 Hz, 1H), 7.96 (s, 1H), 7.23 (dd, J=4.77, 8.03 Hz, 1H), 6.89 (s, 1H), 4.16 (t, J=5.27 Hz, 2H), 3.67-3.95 (m, 6H), 3.62 (t, J=5.14 Hz, 2H), 1.75-1.93 (m, 4H), 1.50-1.65 (m, 4H); LCMS (m/z): 398.1 [M+H]⁺.

Example 170 2-(Dimethylamino)ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[N-methyl-N-(2-thienylmethyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

A solution of the compound (0.10 g, 0.25 mmol) of Example 108, 2-(dimethylamino)ethanol (0.40 mL, 4.0 mmol), 4-dimethylaminopyridine (0.0060 g, 0.049 mmol) and zinc cluster catalyst (Zn₄(OCOCF₃)₆O) (0.012 g, 0.012 mmol) in N,N-dimethylacetamide (0.5 mL) was stirred with the microwave synthesizer (Biotage Initiator™) at 150° C. for 30 min. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC to afford the titled compound as solid (0.0023 g, y. 2%).

¹H NMR (DMSO-d₆) δ (ppm) 12.33 (br. s, 1H), 8.38 (d, J=8.03 Hz, 1H), 8.28-8.32 (m, 1H), 8.03 (s, 1H), 7.53 (dd, J=1.00, 5.02 Hz, 1H), 7.14-7.22 (m, 2H), 7.04 (dd, J=3.51, 5.02 Hz, 1H), 6.92 (s, 1H), 5.20 (s, 2H), 4.21 (t, J=5.90 Hz, 2H), 3.51 (s, 3H), 3.25-3.29 (m, 2H), 2.18 (s, 6H); LCMS (m/z): 453.1 [M+H]⁺.

Example 171 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-({2-methyl-4-[2-(2-pyridinyl)ethoxy]phenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate

First Step

To a solution of 4-nitro-m-cresol (3.0 g, 0.020 mmol) and triphenylphosphine (7.7 g, 0.029 mmol) in tetrahydrofuran (50 mL) cooled with ice bath, diisopropyl azodicarboxylate (5.8 mL, 0.030 mmol) was added dropwise. The mixture was stirred at ambient temperature for 30 min. The reaction mixture was cooled with ice bath, and a solution of 2-pyridineethanol (2.6 mL, 0.024 mmol) in tetrahydrofuran (5.0 mL) was added dropwise to the reaction mixture then the mixture was stirred at ambient temperature for further 16 h. The solvent was removed under reduced pressure, then the residue was purified by chromatography on silica gel(hexane/ethyl acetate) to afford 2-methyl-4-[2-(2-pyridinyl)ethoxy]-1-nitrobenzene as solid (2.0 g, y. 40%).

¹H NMR (DMSO-d₆) δ (ppm) 8.43-8.59 (m, 1H), 8.03 (d, J=9.08 Hz, 1H), 7.73 (t, J=7.62 Hz, 1H), 7.37 (d, J=7.80 Hz, 1H), 7.25 (t, J=6.12 Hz, 1H), 7.03 (s, 1H), 6.98 (d, J=9.20 Hz, 1H), 4.49 (t, J=6.46 Hz, 2H), 3.22 (t, J=6.54 Hz, 2H), 2.53 (s, 3H); LCMS (m/z): 259.0 [M+H]⁺.

Second Step

Under a nitrogen atmosphere, 2-methyl-4-[2-(2-pyridinyl)ethoxy]-1-nitrobenzene (2.0 g, 7.7 mmol) was dissolved in tetrahydrofuran (40 mL) and 10% palladium on carbon (0.20 g) was added at ambient temperature. The reaction mixture was agitated under a hydrogen atmosphere at ambient temperature for 4 h. Palladium on carbon was removed by filtration with Celite and the solvent was removed under reduced pressure to afford 2-methyl-4-[2-(2-pyridinyl)ethoxy]aniline as solid (1.4 g, y. 80%).

¹H NMR (DMSO-d₆) δ (ppm) 8.49 (d, J=4.36 Hz, 1H), 7.71 (t, J=7.64 Hz, 1H), 7.33 (d, J=7.76 Hz, 1H), 7.22 (t, J=6.10 Hz, 1H), 6.45-6.58 (m, 3H), 4.35 (br. s, 2H), 4.18 (t, J=6.66 Hz, 2H), 3.10 (t, J=6.62 Hz, 2H), 2.00 (s, 3H); LCMS (m/z): 229.4 [M+H]⁺.

Third Step

A solution of ethyl 2-ethoxy-4-oxo-4,5-dihydrofuran-3-carboxylate (0.35 g, 1.8 mmol) which afforded in the Example 74, Third step and 2-methyl-4-[2-(2-pyridinyl)ethoxy]aniline (0.40 g, 1.8 mmol) in ethanol (5.0 mL) was stirred at ambient temperature for 1.5 days. The solvent was removed under reduced pressure, then the residue was purified by preparative HPLC to afford ethyl 2-({2-methyl-4-[2-(2-pyridinyl)ethoxy]phenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate as solid (0.15 g, y. 22%).

¹H NMR (DMSO-d₆) δ (ppm) 9.98 (br. s, 1H), 8.51 (d, J=4.52 Hz, 1H), 7.70-7.78 (m, 1H), 7.37 (d, J=7.92 Hz, 1H), 7.22-7.28 (m, 2H), 6.87-6.92 (m, 1H), 6.77-6.84 (m, 1H), 4.57 (s, 2H), 4.36 (t, J=6.62 Hz, 2H), 4.21 (q, J=7.06 Hz, 2H), 3.13-3.25 (m, 2H), 2.19 (s, 3H), 1.21 (s, 3H); LCMS (m/z): 383.2 [M+H]⁺.

Fourth Step

To a stirred solution of ethyl 2-({2-methyl-4-[2-(2-pyridinyl)ethoxy]phenyl}amino)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.15 g, 0.39 mmol) and 7-azaindole-3-carboxaldehyde (0.057 g, 0.39 mmol) in ethanol (5.0 mL), L-proline (5.0 mg, 0.043 mmol) was added at ambient temperature. The mixture was refluxed for 3 days. The solvent was removed under reduced pressure and the residue was purified by preparative HPLC to afford the titled compound as solid (0.015 g, y. 8%).

¹H NMR (DMSO-d₆) δ (ppm) 8.57 (d, J=3.91 Hz, 1H), 8.11 (d, J=3.91 Hz, 1H), 7.78 (t, J=7.58 Hz, 1H), 7.65-7.73 (m, 2H), 7.44 (d, J=7.82 Hz, 1H), 7.24-7.33 (m, 2H), 6.97 (br. s, 1H), 6.88 (d, J=8.31 Hz, 1H), 6.63-6.72 (m, 2H), 4.45 (t, J=6.36 Hz, 2H), 4.21 (d, J=6.85 Hz, 2H), 3.23-3.27 (m, 2H), 2.17 (s, 3H), 1.27 (t, J=6.85 Hz, 3H); LCMS (m/z): 511.2 [M+H]⁺.

Example 172 2-Hydroxyethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(2-chlorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

A solution of the compound (0.10 g, 0.24 mmol) of Example 15, ethylene glycol (0.14 mL, 2.5 mmol) and zinc cluster catalyst (Zn₄(OCOCF₃)₆O) (0.012 g, 0.012 mmol) in N,N-dimethylacetamide (2.0 mL) was stirred with the microwave synthesizer (Biotage Initiator™) at 130° C. for 1 h. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC to afford the titled compound as solid (0.030 g, y. 29%).

¹H NMR (DMSO-d₆) δ (ppm) 12.11 (br. s, 1H), 8.18 (d, J=4.24 Hz, 1H), 7.82 (d, J=7.34 Hz, 1H), 7.59-7.66 (m, 2H), 7.29-7.55 (m, 3H), 6.79 (dd, J=4.65, 7.58 Hz, 1H), 6.62 (br. s, 1H), 4.12-4.22 (m, 2H), 3.65 (t, J=4.89 Hz, 2H); LCMS (m/z): 426 [M+H]⁺.

Example 173 2-Methoxyethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(2-chlorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 172.

¹H NMR (DMSO-d₆) δ (ppm) 12.12 (br. s, 1H), 8.18 (d, J=4.04 Hz, 1H), 7.82 (d, J=7.34 Hz, 1H), 7.58-7.67 (m, 2H), 7.32-7.54 (m, 3H), 6.79 (dd, J=4.65, 7.58 Hz, 1H), 6.63 (br. s, 1H), 4.22-4.32 (m, 2H), 3.56-3.66 (m, 2H), 3.30 (s, 3H); LCMS (m/z): 440.2 [M+H]⁺.

Example 174 3-Hydroxypropyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(2-chlorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 172.

¹H NMR (DMSO-d₆) δ (ppm) 12.23 (br. s, 1H), 10.70 (br. s, 1H), 8.20 (d, J=3.42 Hz, 1H), 7.62-7.85 (m, 4H), 7.43-7.58 (m, 2H), 6.73-6.83 (m, 2H), 4.72 (br. s, 1H), 4.20-4.30 (m, 2H), 3.58 (t, J=5.38 Hz, 2H), 1.75-1.89 (m, 2H); LCMS (m/z): 440.2 [M+H]⁺.

Example 175 (1R,2S)-2-Hydroxycyclopentyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

A solution of the compound (0.10 g, 0.25 mmol) of Example 21, (1R,2S)-1,2-cyclopentanediol (0.26 g, 2.5 mmol) and zinc cluster catalyst (Zn₄(OCOCF₃)₆O) (0.012 g, 0.012 mmol) in N,N-dimethylacetamide (1.5 mL) was stirred with the microwave synthesizer (Biotage Initiator™) at 130° C. for 2 h. Cooled to ambient temperature, the precipitate was removed by filtration. The filtrate was purified by preparative HPLC to afford the titled compound as solid (0.025 g, y. 20%).

¹H NMR (DMSO-d₆) δ (ppm) 12.26 (br. s, 1H), 10.60 (br. s, 1H), 8.26 (d, J=4.40 Hz, 1H), 8.05 (d, J=7.82 Hz, 1H), 7.77 (br. s, 1H), 7.52-7.61 (m, 2H), 7.36 (t, J=8.31 Hz, 2H), 6.94 (dd, J=4.65, 7.58 Hz, 1H), 6.87 (br. s, 1H), 5.37 (br. s, 1H), 4.88-4.98 (m, 1H), 4.09-4.18 (m, 1H), 1.94-2.06 (m, 1H), 1.70-1.90 (m, 3H), 1.43-1.69 (m, 2H); LCMS (m/z): 450.2 [M+H]⁺.

Example 176 4-Hydroxycyclohexyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 175.

¹H NMR (DMSO-d₆) δ (ppm) 12.26 (br. s, 1H), 10.65 (br. s, 1H), 8.25 (d, J=2.93 Hz, 1H), 7.95 (d, J=7.34 Hz, 1H), 7.75 (br. s, 1H), 7.55-7.65 (br. s, 2H), 7.36 (t, J=8.07 Hz, 2H), 6.87-6.94 (m, 1H), 6.82 (br. s, 1H), 4.94 (br. s, 1H), 4.53 (br. s, 1H), 3.56-3.68 (m, 1H), 1.79-1.95 (m, 2H), 1.46-1.77 (m, 6H); LCMS (m/z): 463.8 [M+H]⁺.

Example 177 3-Hydroxy-2,2-dimethylpropyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

A solution of the compound (0.10 g, 0.25 mmol) of Example 21, 2,2-dimethyl-1,3-propanediol (0.40 g, 3.8 mmol) and zinc cluster catalyst (Zn₄(OCOCF₃)₆O) (0.018 g, 0.019 mmol) in N,N-dimethylacetamide (1.5 mL) was stirred with the microwave synthesizer (Biotage Initiator™) at 130° C. for 24 h. Cooled to ambient temperature, the precipitate was removed by filtration. The filtrate was purified by preparative HPLC to afford the titled compound as solid (0.020 g, y. 17%).

¹H NMR (DMSO-d₆) δ (ppm) 12.27 (br. s, 1H), 10.68 (br. s, 1H), 8.25 (d, J=3.91 Hz, 1H), 7.97 (d, J=7.82 Hz, 1H), 7.74 (br. s, 1H), 7.57-7.65 (m, 2H), 7.37 (t, J=8.31 Hz, 2H), 6.83-6.97 (m, 2H), 4.88 (br. s, 1H), 3.93-4.02 (m, 2H), 3.26-3.36 (m, 2H), 0.93 (s, 6H); LCMS (m/z): 452.4 [M+H]⁺.

Example 178 2-(1-Pyrrolidinyl)ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate formate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 177.

¹H NMR (DMSO-d₆) δ (ppm) 12.11 (br. s, 1H), 8.21 (d, J=4.12 Hz, 1H), 8.17 (s, 1H), 7.96 (d, J=7.83 Hz, 1H), 7.65 (s, 1H), 7.34-7.43 (m, 2H), 7.24-7.33 (m, 2H), 6.87 (dd, J=4.89, 7.34 Hz, 1H), 6.65 (s, 1H), 4.25-4.38 (m, 2H), 3.03-3.12 (m, 2H), 2.87-3.00 (m, 4H), 1.65-1.80 (m, 4H); LCMS (m/z): 463.2 [M+H]⁺.

Example 179 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-methyl-1-piperazinyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-[(4-methyl-1-piperazinyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.080 g, 0.30 mmol) which similarly prepared according to the procedure described in the Example 74, Fourth step and 7-azaindole-3-carboxaldehyde (0.040 g, 0.30 mmol) in ethanol (2.0 mL), L-proline (0.020 g, 0.17 mmol) was added at ambient temperature. The mixture was refluxed for 1.5 days. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol and hexane then dried to afford the titled compound as solid (0.017 g, y. 14%).

¹H NMR (DMSO-d₆) δ (ppm) 12.36 (br. s, 1H), 9.95 (br. s, 1H), 8.96 (d, J=7.34 Hz, 1H), 8.35 (d, J=3.42 Hz, 1H), 8.11 (br. s, 1H), 7.23 (dd, J=4.65, 7.58 Hz, 1H), 6.85 (br. s, 1H), 4.21 (q, J=7.30 Hz, 2H), 3.06 (br. s, 3H), 2.43-2.70 (m, 6H), 2.24-2.35 (m, 2H), 1.26 (t, J=6.85 Hz, 3H); LCMS (m/z): 398.2 [M+H]⁺.

Example 180 1-Methyl-4-piperidinyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate formate

A solution of the compound (0.10 g, 0.25 mmol) (of Example 21, 4-hydroxy-1-methylpiperidine (0.44 g, 3.8 mmol) and zinc cluster catalyst (Zn₄(OCOCF₃)₆O) (0.018 g, 0.019 mmol) in N,N-dimethylacetamide (1.0 mL) was stirred at 180° C. for 16 h then at 130° C. for further 16 h. Cooled to ambient temperature, the precipitate was removed by filtration. The filtrate was purified by preparative HPLC to afford the titled compound as solid (0.016 g, y. 13%).

¹H NMR (DMSO-d₆) δ (ppm) 12.15 (br. s, 1H), 8.23 (d, J=3.91 Hz, 1H), 8.18 (s, 1H), 7.96 (d, J=7.82 Hz, 1H), 7.69 (s, 1H), 7.42-7.49 (m, 2H), 7.25-7.36 (m, 2H), 6.89 (dd, J=4.89, 7.83 Hz, 1H), 6.68 (s, 1H), 4.83-4.93 (m, 1H), 2.77-2.87 (m, 2H), 2.35-2.45 (m, 2H), 2.30 (s, 3H), 1.85-1.95 (m, 2H), 1.68-1.80 (m, 2H); LCMS (m/z): 463.2 [M+H]⁺.

Example 181 Isobutyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate formate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 180.

¹H NMR (DMSO-d₆) δ (ppm) 12.17 (br. s, 1H), 8.23 (d, J=3.91 Hz, 1H), 8.19 (s, 1H), 7.96 (d, J=7.82 Hz, 1H), 7.70 (s, 1H), 7.43-7.54 (m, 2H), 7.31 (t, J=8.07 Hz, 2H), 6.84-6.96 (m, 1H), 6.72 (br. s, 1H), 3.96 (d, J=5.87 Hz, 2H), 1.90-2.05 (m, 1H), 0.97 (d, J=6.36 Hz, 6H); LCMS (m/z): 422.2 [M+H]⁺.

Example 182 2-(Dimethylamino)ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(2-chlorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate acetate

A solution of the compound (0.10 g, 0.24 mmol) of Example 15, 2-(dimethylamino)ethanol (0.40 mL, 4.0 mmol), 4-dimethylaminopyridine (0.010 g, 0.082 mmol) and zinc cluster catalyst (Zn₄(OCOCF₃)₆O) (0.018 g, 0.019 mmol) in N,N-dimethylacetamide (2.0 mL) was stirred at 130° C. for 24 h. Cooled to ambient temperature, the precipitate was removed by filtration. The filtrate was purified by preparative HPLC (aqueous ammonium acetate/acetonitrile as eluents) to afford the titled compound as solid (0.012 g, y. 11%).

¹H NMR (DMSO-d₆) δ (ppm) 11.92 (br. s, 1H), 8.15 (d, J=3.91 Hz, 1H), 7.86 (d, J=7.82 Hz, 1H), 7.48-7.55 (m, 2H), 7.30-7.37 (m, 1H), 7.15-7.23 (m, 2H), 6.73-6.79 (m, 1H), 6.39 (s, 1H), 4.30-4.39 (m, 2H), 3.12-3.22 (m, 2H), 2.73 (br. s, 6H), 1.91 (s, 3H); LCMS (m/z): 453.0 [M+H]⁺.

Example 183 3-Hydroxycyclohexyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

A solution of the compound (0.10 g, 0.25 mmol) of Example 21, 1,3-cyclohexanediol (0.44 g, 3.8 mmol) and zinc cluster catalyst (Zn₄(OCOCF₃)₆O) (0.018 g, 0.019 mmol) in N,N-dimethylacetamide (1.0 mL) was stirred at 130° C. for 1.5 days. Cooled to ambient temperature, the precipitate was removed by filtration. The filtrate was purified by preparative HPLC to afford the titled compound as solid (0.010 g, y. 13%).

¹H NMR (DMSO-d₆) δ (ppm) 12.25 (br. s, 1H), 8.24 (d, J=3.91 Hz, 1H), 7.92-8.00 (m, 1H), 7.74 (br. s, 1H), 7.55-7.65 (m, 2H), 7.31-7.42 (m, 2H), 6.90 (dd, J=4.89, 7.82 Hz, 1H), 6.78-6.86 (m, 1H), 5.19-5.28 (m, 1H), 4.92-5.02 (m, 1H), 4.55 (d, J=3.42 Hz, 1H), 1.15-2.08 (m, 8H); LCMS (m/z): 463.8 [M+H]⁺.

Example 184 (1-Methyl-2-piperidinyl)methyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate formate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 183.

¹H NMR (DMSO-d₆) δ (ppm) 12.11 (br. s, 1H), 8.22 (d, J=4.40 Hz, 1H), 8.16 (s, 1H), 7.98 (d, J=7.82 Hz, 1H), 7.66 (s, 1H), 7.34-7.42 (m, 2H), 7.25-7.33 (m, 2H), 6.88 (dd, J=4.65, 7.58 Hz, 1H), 6.65 (s, 1H), 4.23-4.35 (m, 2H), 2.90-3.00 (m, 1H), 2.62-2.72 (m, 1H), 2.46-2.56 (m, 4H), 1.18-1.83 (m, 6H); LCMS (m/z): 477.4 [M+H]⁺.

Example 185 2-(4-Methyl-1-piperazinyl)ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate formate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 183.

¹H NMR (DMSO-d₆) δ (ppm) 12.14 (br. s, 1H), 8.16-8.25 (m, 2H), 7.95 (d, J=6.85 Hz, 1H), 7.67 (br. s, 1H), 7.38-7.48 (m, 2H), 7.25-7.36 (m, 2H), 6.84-6.91 (m, 1H), 6.68 (br. s, 1H), 4.20-4.34 (m, 2H), 2.22-2.71 (m, 10H), 2.13 (br. s, 3H); LCMS (m/z): 492.4 [M+H]⁺.

Example 186 2-(N,N-Dimethylamino)-2-methylpropyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate formate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 183.

¹H NMR (DMSO-d₆) δ (ppm) 12.01 (br. s, 1H), 8.15-8.29 (m, 2H), 7.98 (d, J=7.34 Hz, 1H), 7.60 (br. s, 1H), 7.05-7.33 (m, 4H), 6.83-6.93 (m, 1H), 6.47 (s, 1H), 4.20 (br. s, 2H), 2.55 (br. s, 6H), 1.22 (br. s, 6H); LCMS (m/z): 465.0 [M+H]⁺.

Example 187 (1-Methyl-4-piperidinyl)methyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(4-fluorophenyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate formate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 183.

¹H NMR (DMSO-d₆) δ (ppm) 12.09 (br. s, 1H), 8.18-8.24 (m, 2H), 7.96 (d, J=7.82 Hz, 1H), 7.66 (s, 1H), 7.33-7.43 (m, 2H), 7.22-7.32 (m, 2H), 6.89 (dd, J=4.89, 7.83 Hz, 2H), 6.59 (s, 1H), 4.01 (d, J=6.36 Hz, 2H), 2.88-2.96 (m, 2H), 2.29 (s, 3H), 2.03-2.15 (m, 2H), 1.62-1.82 (m, 3H), 1.26-1.41 (m, 2H); LCMS (m/z): 477.3 [M+H]⁺.

Example 188 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(4-morpholinopiperidin-1-yl)-4-oxo-4,5-dihydrofuran-3-carboxylate

To a stirred solution of ethyl 2-(4-morpholinopiperidin-1-yl)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.10 g, 0.31 mmol) which similarly prepared according to the procedure described in the Example 74, Fourth step and 7-azaindole-3-carboxaldehyde (0.045 g, 0.31 mmol) in ethanol (10 mL), piperidine (0.020 mL, 0.20 mmol) was added at ambient temperature. The mixture was refluxed for 16 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol and hexane then dried to afford the titled compound (0.018 g, y. 13%) as solid.

¹H NMR (DMSO-d₆) δ (ppm) 12.31 (br. s, 1H), 8.37 (d, J=7.34 Hz, 1H), 8.31 (d, J=3.42 Hz, 1H), 7.97 (br. s, 1H), 7.16-7.23 (m, 1H), 6.85 (s, 1H), 4.11-4.30 (m, 4H), 3.52-3.64 (m, 4H), 3.23-3.43 (m, 7H), 1.90-2.02 (m, 2H), 1.54-1.76 (m, 2H), 1.24 (t, J=6.60 Hz, 3H); LCMS (m/z): 453.2 [M+H]⁺.

Example 189 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(3-carbamoylpiperidino)-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-(3-carbamoylpiperidino)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.15 g, 0.53 mmol) which similarly prepared according to the procedure described in the Example 74, Fourth step and 7-azaindole-3-carboxaldehyde (0.078 g, 0.53 mmol) in ethanol (10 mL), piperidine (0.020 mL, 0.20 mmol) was added at ambient temperature. The mixture was refluxed for 16 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol and hexane then dried to afford the titled compound as solid (0.018 g, y. 9%).

¹H NMR (DMSO-d₆) δ (ppm) 12.34 (br. s, 1H), 8.38 (d, J=7.52 Hz, 1H), 8.31 (d, J=3.72 Hz, 1H), 7.97 (br. s, 1H), 7.44 (br. s, 1H), 7.13-7.23 (m, 1H), 6.99 (br. s, 1H), 6.85 (s, 1H), 4.10-4.22 (m, 4H), 3.25-3.46 (m, 2H), 2.54-2.64 (m, 1H), 1.96-2.06 (m, 1H), 1.82-1.92 (m, 1H), 1.60-1.74 (m, 2H), 1.24 (t, J=6.85 Hz, 3H); LCMS (m/z): 411.4 [M+H]⁺.

Example 190 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-4-oxo-2-(3-oxopiperazino)-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 189.

¹H NMR (DMSO-d₆) δ (ppm) 12.38 (br. s, 1H), 8.29-8.44 (m, 2H), 8.01 (br. s, 1H), 7.15-7.25 (m, 1H), 6.92 (br. s, 1H), 4.30-4.40 (m, 2H), 4.11-4.23 (m, 2H), 3.92-4.04 (m, 2H), 3.41-3.49 (m, 2H), 1.18-1.31 (m, 3H); LCMS (m/z): 383.0 [M+H]⁺.

Example 191 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(4-acetyl-1,4-diazepanyl)-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 189.

¹H NMR (DMSO-d₆) δ (ppm) 12.36 (br. s, 1H), 8.41 (d, J=7.68 Hz, 1H), 8.35 (d, J=4.36 Hz, 1H), 7.99 (br. s, 1H), 7.17-7.29 (m, 1H), 6.89 (d, J=3.91 Hz, 1H), 4.15-4.27 (m, 2H), 3.92-4.10 (m, 3H), 3.73-3.90 (m, 3H), 3.51-3.64 (m, 2H), 1.83-2.08 (m, 5H), 1.28 (t, J=6.60 Hz, 3H); LCMS (m/z): 425.3 [M+H]⁺.

Example 192 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(4-methyl-1,4-diazepanyl)-4-oxo-4,5-dihydrofuran-3-carboxylate acetate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 189. Further, preparative HPLC (aqueous ammonium acetate/acetonitrile) was used as purification procedure.

¹H NMR (DMSO-d₆) δ (ppm) 12.48 (br. s, 1H), 8.36 (d, J=7.82 Hz, 1H), 8.30 (d, J=4.40 Hz, 1H), 7.96 (s, 1H), 7.18 (dd, J=4.89, 7.82 Hz, 1H), 6.85 (s, 1H), 4.16 (q, J=7.34 Hz, 2H), 3.70-3.92 (m, 4H), 2.72-2.82 (m, 2H), 2.53-2.63 (m, 2H), 2.28 (s, 3H), 1.92-2.02 (m, 2H), 1.62 (s, 3H), 1.24 (t, J=7.09 Hz, 3H); LCMS (m/z): 397.2 [M+H]⁺.

Example 193 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(2-methyl-2-phenylhydrazinyl)-4-oxo-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 2-(2-methyl-2-phenylhydrazinyl)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.10 g, 0.36 mmol) which similarly prepared according to the procedure described in the Example 74, Fourth step and 7-azaindole-3-carboxaldehyde (0.053 g, 0.36 mmol) in ethanol (10 mL), L-proline (0.020 g, 0.17 mmol) was added at ambient temperature. The mixture was refluxed for 2 days. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol and hexane then dried to afford the titled compound as solid (0.015 g, y. 10%).

¹H NMR (DMSO-d₆) δ (ppm) 12.28 (br. s, 1H), 10.79 (s, 1H), 8.48 (d, J=8.31 Hz, 1H), 8.22 (d, J=3.91 Hz, 1H), 7.90 (br. s, 1H), 7.32 (t, J=7.82 Hz, 2H), 6.83-7.05 (m, 5H), 4.25 (q, J=6.85 Hz, 2H), 3.34 (s, 3H), 1.29 (t, J=6.85 Hz, 3H); LCMS (m/z): 405.2 [M+H]⁺.

Example 194 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[4-(diethylamino)piperidino]-4-oxo-4,5-dihydrofuran-3-carboxylate

First Step

Under a nitrogen atmosphere, to a solution of tert-butyl 4-oxopiperidine-1-carboxylate (5.0 g, 0.025 mol) and diethylamine (9.1 mL, 0.090 mol) in methanol (40 mL), 10% palladium on carbon (0.50 g) was added at ambient temperature. The reaction mixture was agitated under a hydrogen atmosphere at ambient temperature for 16 h. Palladium on carbon was removed by filtration with Celite and the solvent was removed under reduced pressure, then the residue was purified by chromatography on silica gel(dichloromethane/methanol) to afford tert-butyl 4-(diethylamino)piperidine-1-carboxylate as solid (6.1 g, y. 95%).

¹H NMR (CDCl₃) δ (ppm) 4.12 (br. s, 2H), 3.76-3.89 (m, 1H), 2.45-2.72 (m, 6H), 1.30-1.53 (m, 13H), 0.90-1.10 (m, 6H)

Second Step

Cooled with ice bath, 4M hydrochloric acid in dioxane (25 mL) was added to tert-butyl 4-(N,N-diethylamino)piperidine-1-carboxylate (6.1 g, 0.024 mol) and the mixture was stirred at ambient temperature for 2 h. The solvent was removed under reduced pressure, and the residue was suspended in diethyl ether, then the precipitate was collected by filtration, washed with diethyl ether and then dried to afford 4-(diethylamino)piperidine dihydrochloride as solid (3.9 g, y. 99%).

Third Step

To a solution of 4-(diethylamino)piperidine dihydrochloride (3.0 g, 0.019 mmol) in water (20 mL), 2M sodium hydroxide solution (5.0 mL) was added at ambient temperature. The mixture was stirred for 30 min. The solvent was removed under reduced pressure, and methanol (10 mL) was added to the crude material, then the residue was removed by filtration. The filtrate was concentrated and dried to afford 4-(diethylamino)piperidine (1.7 g, y. 77%) as solid.

¹H NMR (DMSO-d₆) δ (ppm) 3.20-3.29 (m, 2H), 3.09-3.14 (m, 1H), 2.77-2.93 (m, 3H), 2.52-2.62 (m, 4H), 1.64-1.90 (m, 4H), 1.00 (t, J=7.04 Hz, 6H)

Fourth Step

A solution of 4-(diethylamino)piperidine (0.50 g, 3.2 mmol), ethyl 2-ethoxy-4-oxo-4,5-dihydrofuran-3-carboxylate (0.76 g, 3.8 mmol) which afforded in the Example 74, Third step and triethylamine (0.60 mL, 4.3 mmol) in tetrahydrofuran (10 mL) was stirred at ambient temperature for 24 h. The solvent was removed under reduced pressure, then the crude material was purified by chromatography on silica gel (dichloromethane/methanol) to afford ethyl 2-[4-(diethylamino)piperidino]-4-oxo-4,5-dihydrofuran-3-carboxylate as oil (0.29 g, y. 30%).

¹H NMR (DMSO-d₆) δ (ppm) 4.53 (s, 2H), 4.03-4.15 (m, 4H), 3.05-3.19 (m, 2H), 2.82 (br. s, 1H), 2.42-2.58 (m, 4H), 1.73-1.86 (m, 2H), 1.40-1.59 (m, 2H), 1.20 (t, J=7.08 Hz, 3H), 0.91-1.02 (m, 6H); LCMS (m/z): 311.0 [M+H]⁺

Fifth Step

To a solution of ethyl 2-[4-(diethylamino)piperidino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.10 g, 0.30 mmol) and 7-azaindole-3-carboxaldehyde (0.047 g, 0.30 mmol) in ethanol (5.0 mL), L-proline (0.0040 mg, 0.035 mmol) was added at ambient temperature. The mixture was refluxed for 16 h. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol and hexane then dried to afford the titled compound as solid (0.035 g, y. 25%).

¹H NMR (DMSO-d₆) δ (ppm) 12.30 (br. s, 1H), 8.37 (d, J=7.82 Hz, 1H), 8.31 (d, J=3.91 Hz, 1H), 7.97 (s, 1H), 7.19 (dd, J=4.89, 7.82 Hz, 1H), 6.84 (s, 1H), 4.11-4.33 (m, 4H), 3.27-3.37 (m, 2H), 2.82-2.92 (m, 1H), 2.41-2.60 (m, 4H), 1.82-1.92 (m, 2H), 1.56-1.71 (m, 2H), 1.24 (t, J=6.85 Hz, 3H), 0.98 (t, J=7.09 Hz, 6H); LCMS (m/z): 439.4 [M+H]⁺.

Example 195 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-4-oxo-2-(N-piperidinoamino)-4,5-dihydrofuran-3-carboxylate

To a solution of ethyl 4-oxo-2-(N-piperidinoamino)-4,5-dihydrofuran-3-carboxylate (0.25 g, 0.98 mmol) which similarly prepared according to the procedure described in the Example 74, Fourth step and 7-azaindole-3-carboxaldehyde (0.14 g, 0.98 mmol) in ethanol (10 mL), L-proline (0.011 mg, 0.096 mmol) was added at ambient temperature. The mixture was refluxed for 2 days. Cooled to ambient temperature, the precipitate was collected by filtration, washed with ethanol and diethyl ether then dried to afford the titled compound as solid (0.015 g, y. 4%).

¹H NMR (DMSO-d₆) δ (ppm) 12.34 (br. s, 1H), 9.86 (br. s, 1H), 9.00 (d, J=7.82 Hz, 1H), 8.33 (d, J=3.91 Hz, 1H), 8.10 (d, J=2.45 Hz, 1H), 7.18 (dd, J=4.65, 8.07 Hz, 1H), 6.85 (s, 1H), 4.20 (q, J=6.85 Hz, 2H), 2.95-3.05 (m, 4H), 1.69-1.79 (m, 4H), 1.42-1.53 (m, 2H), 1.26 (t, J=6.85 Hz, 3H); LCMS (m/z): 383.2 [M+H]⁺.

Example 196 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(2,2-dimethylhydrazinyl)-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 195.

¹H NMR (DMSO-d₆) δ (ppm) 11.85 (br. s, 1H), 8.52 (d, J=7.82 Hz, 1H), 8.23 (d, J=3.91 Hz, 1H), 7.83 (s, 1H), 7.10 (dd, J=4.89, 7.82 Hz, 1H), 6.14 (s, 1H), 4.02 (q, J=6.85 Hz, 2H), 1.70 (s, 6H), 1.18 (t, J=7.09 Hz, 3H); LCMS (m/z): 342.8 [M+H]⁺.

Example 197 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{[2-(dimethylamino)ethyl]-N-methylamino}-4-oxo-4,5-dihydrofuran-3-carboxylate formate

To a solution of ethyl 2-{[2-(dimethylamino)ethyl]-N-methylamino}-4-oxo-4,5-dihydrofuran-3-carboxylate (0.20 g, 0.78 mmol) which similarly prepared according to the procedure described in the Example 74, Fourth step and 7-azaindole-3-carboxaldehyde (0.11 g, 0.78 mmol) in ethanol (6.0 mL), L-proline (0.0093 mg, 0.078 mmol) was added at ambient temperature. The mixture was refluxed for 20 h. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC to afford the titled compound as solid (0.035 g, y. 12%).

¹H NMR (DMSO-d₆) δ (ppm) 12.35 (br. s, 1H), 8.38 (d, J=7.83 Hz, 1H), 8.27-8.34 (m, 1H), 8.18 (s, 1H), 7.98 (s, 1H), 7.16-7.22 (m, 1H), 6.85 (s, 1H), 4.17 (q, J=6.85 Hz, 2H), 3.83 (t, J=6.60 Hz, 2H), 3.29 (br. s, 3H), 2.54-2.61 (m, 2H), 2.19 (s, 6H), 1.21-1.28 (m, 3H); LCMS (m/z): 385.0 [M+H]⁺.

Example 198 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(1,4-oxoazepan-4-yl)-4-oxo-4,5-dihydrofuran-3-carboxylate acetate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 197. Further, preparative HPLC (aqueous ammonium acetate/acetonitrile) was used as purification procedure.

¹H NMR (DMSO-d₆) δ (ppm) 8.35 (d, J=7.82 Hz, 1H), 8.30 (d, J=4.40 Hz, 1H), 7.97 (s, 1H), 7.18 (dd, J=4.89, 7.82 Hz, 1H), 6.87 (s, 1H), 4.17 (q, J=7.17 Hz, 2H), 3.80-4.01 (m, 6H), 3.74 (t, J=5.14 Hz, 2H), 1.95-2.07 (m, 2H), 1.63 (s, 3H), 1.24 (t, J=7.09 Hz, 3H); LCMS (m/z): 383.8 [M+H]⁺.

Example 199 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(cis-4-hydroxycyclohexyl)amino]-4-oxo-4,5-dihydrofuran-3-carboxylate

First Step

Cooled with ice bath, to a mixed solution of cyclohexanone oxime (4.0 g, 0.035 mol) in dichloromethane (40 mL) and ethanol (5.0 mL), tert-butyl hypochlorite (4.7 mL, 0.038 mol) was added dropwise and the reaction mixture was cooled to −20° C. then stirred for 30 min. To this reaction mixture, 1,3-cyclohexadiene (5.0 mL, 0.052 mol) was added dropwise and the reaction mixture was allowed to warm to 0° C. then stirred for 4 days. The reaction mixture was concentrated under reduced pressure to half the solvents, and then diethyl ether (80 mL) was added at ambient temperature then the mixture was stirred for further 1 day. To this reaction mixture, ethanol (10 mL) was added and the mixture was stirred for further 4 h, then the precipitate was collected by filtration. The solid was washed with mixed solution of diethyl ether/ethanol (10/1), diethyl ether and hexane then dried to afford 2-oxa-3-azabicyclo[2.2.2]-5-octene hydrochloride as solid (4.2 g, y. 82%).

¹H NMR (CDCl₃) δ (ppm) 12.43 (br. s, 1H), 11.29 (br. s, 1H), 6.81-6.88 (m, 1H), 6.64-6.71 (m, 1H), 4.87-4.93 (m, 1H), 4.58-4.65 (m, 1H), 2.54-2.66 (m, 1H), 2.31-2.47 (m, 1H), 1.45-1.59 (m, 2H)

Second Step

Under a nitrogen atmosphere, to a mixed solution of 2-oxa-3-azabicyclo[2.2.2]-5-octene hydrochloride (0.20 g, 1.4 mol) in ethanol (2.0 mL) and methanol (1.0 mL), platinum oxide (0.12 g, 0.53 mmol) was added at ambient temperature. The reaction mixture was agitated under a hydrogen atmosphere at ambient temperature for 6.5 h. Platinum oxide was removed by filtration with Celite, cooled this filtrate with ice bath, and 2M hydrochloric acid in ethanol (1.6 mL, 3.2 mmol) was added then the mixture was stirred for 10 min. The solvent was removed under reduced pressure, then 2-propanol (2.0 mL) and ethanol (0.20 mL) were added to the residue then the mixture was refluxed for 40 min and filtered. The filtrate was refluxed for further 10 min, stirred at ambient temperature for 14 h, then cooled with ice bath and stirred for further 30 min. The precipitate was collected by filtration, washed with ice-cold 2-propylalcohol and hexane then dried to afford cis-4-aminocyclohexanol hydrochloride as solid (0.056 g, y. 27%).

¹H NMR (DMSO-d₆) δ (ppm) 7.92 (br. s, 3H), 4.47 (br. s, 1H), 3.70-3.82 (m, 1H), 2.89-3.04 (m, 1H), 1.57-1.76 (m, 6H), 1.37-1.51 (m, 2H)

Third Step

To a solution of cis-4-aminocyclohexanol hydrochloride (0.051 g, 0.34 mmol) and ethyl 2-ethoxy-4-oxo-4,5-dihydrofuran-3-carboxylate (0.073 g, 0.37 mmol) which afforded in the Example 74, Third step in ethanol (0.8 mL), triethylamine (0.052 mL, 0.37 mmol) was added. The mixture was stirred at ambient temperature for 1 h. To this reaction mixture, 7-azaindole-3-carboxaldehyde (0.044 g, 0.30 mmol) and piperidine (0.0067 mL, 0.067 mmol) was added at ambient temperature then the mixture was refluxed for 4 days. Ethanol (2.0 mL) was added then the mixture was refluxed for further 1 h, the precipitate was collected by filtration, washed with hot ethanol. The solid was washed with hexane then dried to afford the titled compound as solid (0.013 g, y. 11%).

¹H NMR (DMSO-d₆) δ (ppm) 12.33 (br. s, 1H), 8.50 (d, J=8.03 Hz, 1H), 8.42 (d, J=7.78 Hz, 1H), 8.32 (dd, J=1.38, 4.64 Hz, 1H), 8.02 (d, J=2.51 Hz, 1H), 7.20 (dd, J=4.64, 7.91 Hz, 1H), 6.90 (s, 1H), 4.55 (d, J=3.26 Hz, 1H), 4.22 (q, J=7.03 Hz, 2H), 4.03-4.14 (m, 1H), 3.74-3.82 (m, 1H), 1.90-2.02 (m, 2H), 1.72-1.81 (m, 2H), 1.61-1.69 (m, 4H), 1.26 (t, J=7.15 Hz, 3H); LCMS (m/z): 396.1 [M+H]⁺.

Example 200 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[4-(dimethylamino)piperidino]-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 194.

¹H NMR (DMSO-d₆) δ (ppm) 12.37 (br. s, 1H), 8.39 (d, J=7.00 Hz, 1H), 8.33 (d, J=5.16 Hz, 1H), 8.01 (s, 1H), 7.21 (dd, J=5.12, 7.72 Hz, 1H), 6.89 (s, 1H), 4.35-4.45 (m, 2H), 4.13-4.23 (m, 2H), 3.88-3.98 (m, 1H), 3.61-3.71 (m, 1H), 3.51-3.61 (m, 1H), 2.50 (s, 6H), 2.18-2.28 (m, 1H), 1.95-2.05 (m, 1H), 1.80-1.95 (m, 1H), 1.25 (t, J=6.96 Hz, 3H); LCMS (m/z): 411.2 [M+H]⁺.

Example 201 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-({2-[(2-methoxyethoxy)methoxy]ethyl}-N-methylamino)-4-oxo-4,5-dihydrofuran-3-carboxylate

First Step

To a solution of ethyl 2-[(2-hydroxyethyl)-N-methylamino]-4-oxo-4,5-dihydrofuran-3-carboxylate (0.5 g, 2.18 mmol) which similarly prepared according to the procedure described in the Example 74, Fourth step in dichloromethane (6.0 mL) that cooled with ice bath, diisopropylethylamine (0.75 mL, 4.36 mmol) then 2-methoxyethoxymethyl chloride (0.5 mL, 4.36 mmol) were added. The reaction mixture was allowed to warm to ambient temperature and stirred for 16 h. The reaction mixture was diluted with water, extracted with ethyl acetate for 3 times. The organic layer was dried over sodium sulfate and concentrated. The residue was purified by chromatography on silica gel(hexane/ethyl acetate) to afford ethyl 2-({2-[(2-methoxyethoxy)methoxy]ethyl}-N-methylamino)-4-oxo-4,5-dihydrofuran-3-carboxylate as oil (0.31 g, y. 46%).

¹H NMR (DMSO-d₆) δ (ppm) 4.62 (s, 2H), 4.54 (s, 2H), 4.10 (q, J=7.04 Hz, 2H), 3.63-3.80 (m, 4H), 3.52-3.62 (m, 2H), 3.38-3.48 (m, 2H), 3.24 (s, 3H), 3.12 (s, 3H), 1.20 (t, J=7.04 Hz, 3H); LCMS (m/z): 230.0 [M+H]⁺

Second Step

To a solution of ethyl 2-({2-[(2-methoxyethoxy)methoxy]ethyl}-N-methylamino)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.146 g, 0.46 mmol) and 7-azaindole-3-carboxaldehyde (0.067 g, 0.46 mmol) in ethanol (6.0 mL), L-proline (0.005 g, 0.046 mmol) was added at ambient temperature. The mixture was refluxed for 16 h. Cooled to ambient temperature, the reaction mixture was purified by chromatography on silica gel(hexane/ethyl acetate) to afford the titled compound as solid (0.035 g, y. 17%).

¹H NMR (DMSO-d₆) δ (ppm) 12.33 (br. s, 1H), 8.39 (d, J=7.34 Hz, 1H), 8.31 (d, J=4.40 Hz, 1H), 7.98 (s, 1H), 7.19 (dd, J=4.65, 7.58 Hz, 1H), 6.85 (s, 1H), 4.64 (s, 2H), 4.16 (q, J=7.17 Hz, 2H), 3.93-4.00 (m, 2H), 3.75-3.83 (m, 2H), 3.51-3.59 (m, 2H), 3.36-3.42 (m, 2H), 3.25-3.37 (m, 3H), 3.17 (s, 3H), 1.24 (t, J=7.09 Hz, 3H); LCMS (m/z): 446.2 [M+H]⁺

Example 202 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-[(2-hydroxyethyl)-N-methylamino]-4-oxo-4,5-dihydrofuran-3-carboxylate

Cooled with ice bath, 4M hydrochloric acid in dioxane (4.0 mL) was added to ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-({2-[(2-methoxyethoxy)methoxy]ethyl}-N-methylamino)-4-oxo-4,5-dihydrofuran-3-carboxylate (0.020 mg, 0.046 mmol) which afforded in the Example 201. The mixture was stirred at ambient temperature for 3 h. The solvent was removed under reduced pressure, and the precipitate was suspended in diethyl ether, then collected by filtration. The solid was washed with diethyl ether then dried to afford the titled compound as solid (0.012 g, y. 75%).

¹H NMR (DMSO-d₆) δ (ppm) 12.35 (br. s, 1H), 8.42 (d, J=7.82 Hz, 1H), 8.32 (d, J=3.91 Hz, 1H), 7.99 (s, 1H), 7.21 (dd, J=4.89, 7.82 Hz, 1H), 6.84 (s, 1H), 4.16 (q, J=6.85 Hz, 2H), 3.80-3.86 (m, 2H), 3.65-3.78 (m, 2H), 3.31 (br. s., 3H), 1.24 (t, J=7.09 Hz, 4H); LCMS (m/z): 358.2 [M+H]⁺

Example 203 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-{4-[(2-hydroxyethyl)-N-methylamino]piperidino}-4-oxo-4,5-dihydrofuran-3-carboxylate

The titled compound (solid) was similarly prepared according to the procedure described in the Example 194.

¹H NMR (DMSO-d₆) δ (ppm) 12.30 (br. s, 1H), 8.37 (d, J=7.82 Hz, 1H), 8.31 (d, J=4.40 Hz, 1H), 7.97 (s, 1H), 7.20 (dd, J=4.65, 7.58 Hz, 1H), 6.85 (s, 1H), 4.22-4.38 (m, 4H), 4.17 (q, J=6.85 Hz, 2H), 3.45 (q, J=5.87 Hz, 2H), 3.25-3.40 (m, 2H), 2.69-2.81 (m, 1H), 2.23 (s, 3H), 1.85-1.95 (m, 2H), 1.58-1.72 (m, 2H), 1.25 (t, J=6.85 Hz, 3H); LCMS (m/z): 441.2 [M+H]⁺.

Example 204 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-(phenylamino)furan-3(2H)-one

A solution of the compound (0.050 g, 0.13 mmol) of Example 1 in N,N-dimethylformamide (1.0 mL) was refluxed for 6 h. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC to afford the titled compound as solid (0.010 g, y. 25%).

¹H NMR (DMSO-d₆) δ (ppm) 12.36 (br. s, 1H), 10.74 (s, 1H), 8.42 (d, J=7.53 Hz, 1H), 8.31 (dd, J=1.25, 4.52 Hz, 1H), 8.10 (d, J=2.26 Hz, 1H), 7.41-7.49 (m, 2H), 7.34 (d, J=7.53 Hz, 2H), 7.13-7.25 (m, 2H), 6.81 (s, 1H), 5.25 (s, 1H); LCMS (m/z): 304.0 [M+H]⁺.

Example 205 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(4-methoxyphenyl)amino]furan-3(2H)-one

To a solution of the compound (0.31 g, 0.76 mmol) of Example 2 in ethanol (6.0 mL), 18M potassium hydroxide solution (0.41 mL, 7.4 mmol) was added at ambient temperature. The mixture was refluxed for 24 h. Cooled to ambient temperature, the precipitate was removed by filtration. The filtrate was purified by preparative HPLC to afford the titled compound as solid (0.031 g, y. 12%).

¹H NMR (DMSO-d₆) δ (ppm) 12.31 (br. s, 1H), 10.56 (s, 1H), 8.39 (d, J=7.53 Hz, 1H), 8.30 (dd, J=1.51, 4.52 Hz, 1H), 8.05 (s, 1H), 7.25-7.32 (m, 2H), 7.14 (dd, J=4.64, 7.91 Hz, 1H), 6.99-7.04 (m, 2H), 6.76 (s, 1H), 5.05 (s, 1H), 3.79 (s, 3H); LCMS (m/z): 334.0 [M+H]⁺.

Example 206 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(4-chlorophenyl)amino]furan-3(2H)-one

A solution of the compound (0.050 g, 0.12 mmol) of Example 8 in N,N-dimethylformamide (1.0 mL) was refluxed for 6 h. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC to afford the titled compound as solid (0.0072 g, y. 17%).

¹H NMR (DMSO-d₆) δ (ppm) 12.36 (br. s, 1H), 10.87 (br. s, 1H), 8.41 (d, J=7.28 Hz, 1H), 8.31 (dd, J=1.38, 4.64 Hz, 1H), 8.08 (d, J=2.26 Hz, 1H), 7.48 (d, J=8.78 Hz, 2H), 7.36 (d, J=8.78 Hz, 2H), 7.17 (dd, J=4.64, 7.91 Hz, 1H), 6.82 (s, 1H), 5.27 (s, 1H); LCMS (m/z): 337.9 [M+H]⁺.

Example 207 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(3-chlorophenyl)amino]furan-3(2H)-one

A solution of the compound (0.040 g, 0.098 mmol) of Example 9 in N,N-dimethylformamide (1.0 mL) was refluxed for 6 h. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC to afford the titled compound as solid (0.0075 g, y. 23%).

¹H NMR (DMSO-d₆) δ (ppm) 12.40 (br. s, 1H), 10.85 (s, 1H), 8.41 (d, J=7.28 Hz, 1H), 8.32 (dd, J=1.38, 4.64 Hz, 1H), 8.09 (d, J=2.76 Hz, 1H), 7.42-7.51 (m, 1H), 7.36-7.41 (m, 1H), 7.32 (d, J=8.03 Hz, 1H), 7.26 (dd, J=1.00, 8.03 Hz, 1H), 7.17 (dd, J=4.64, 7.91 Hz, 1H), 6.85 (s, 1H), 5.31 (s, 1H); LCMS (m/z): 337.9 [M+H]⁺.

Example 208 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(2-chlorophenyl)amino]furan-3(2H)-one

A solution of the compound (0.050 g, 0.12 mmol) of Example 15 in N,N-dimethylformamide (1.0 mL) was stirred at 150° C. for 6 h. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC to afford the titled compound as solid (0.0050 g, y. 11%).

¹H NMR (DMSO-d₆) δ (ppm) 12.29 (br. s, 1H), 10.63 (br. s, 1H), 8.21-8.32 (m, 2H), 8.01 (br. s, 1H), 7.63 (d, J=8.03 Hz, 1H), 7.57 (d, J=7.28 Hz, 1H), 7.45 (t, J=7.40 Hz, 1H), 7.32-7.39 (m, 1H), 7.06 (dd, J=4.77, 7.53 Hz, 1H), 6.75 (s, 1H), 4.89 (br. s, 1H); LCMS (m/z): 337.9 [M+H]⁺.

Example 209 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(2,4-dimethoxyphenyl)amino]furan-3(2H)-one

A solution of the compound (0.10 g, 0.23 mmol) of Example 24 in N,N-dimethylformamide (3.0 mL) was refluxed for 7 h. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC to afford the titled compound as solid (0.015 g, y. 18%).

¹H NMR (DMSO-d₆) δ (ppm) 12.27 (br. s, 1H), 10.11 (br. s, 1H), 8.25-8.36 (br. s, 2H), 8.03 (br. s, 1H), 7.30 (d, J=8.31 Hz, 1H), 7.08 (br. s, 1H), 6.72 (d, J=17.12 Hz, 2H), 6.60 (d, J=8.31 Hz, 1H), 4.74 (br. s, 1H), 3.75-3.92 (m, 6H); LCMS (m/z): 364.2 [M+H]⁺.

Example 210 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(4-isopropylphenyl)amino]furan-3(2H)-one

A solution of the compound (0.12 g, 0.29 mmol) of Example 37 in N,N-dimethylformamide (3.0 mL) was refluxed for 6 h. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC to afford 2-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(4-isopropylphenyl)amino]furan-3(2H)-one as solid (0.0050 g, y. 5%).

¹H NMR (DMSO-d₆) δ (ppm) 12.34 (br. s, 1H), 10.68 (br. s, 1H), 8.41 (d, J=7.82 Hz, 1H), 8.30 (d, J=3.91 Hz, 1H), 8.09 (br. s, 1H), 7.21-7.37 (m, 4H), 7.15 (d, J=4.40 Hz, 1H), 6.77 (s, 1H), 5.18 (br. s, 1H), 2.84-3.00 (m, 1H), 1.22 (d, J=6.85 Hz, 6H); LCMS (m/z): 346.2 [M+H]⁺.

Example 211 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-(3-pyridinylamino)furan-3(2H)-one

The titled compound (solid) was similarly prepared according to the procedure described in the Example 210, using the compound of Example 27.

¹H NMR (DMSO-d₆) δ (ppm) 12.35 (br. s, 1H), 10.95 (br. s, 1H), 8.58 (br. s, 1H), 8.35-8.43 (m, 2H), 8.27-8.33 (m, 1H), 8.06 (br. s, 1H), 7.76 (d, J=6.85 Hz, 1H), 7.38-7.53 (m, 1H), 7.09-7.24 (m, 1H), 6.81 (br. s, 1H), 5.27 (br. s, 1H); LCMS (m/z): 305.2 [M+H]⁺.

Example 212 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(4-carbamoylphenyl)amino]furan-3(2H)-one

The titled compound (solid) was similarly prepared according to the procedure described in the Example 210, using the compound of Example 25.

¹H NMR (DMSO-d₆) δ (ppm) 12.37 (br. s, 1H), 8.42 (d, J=7.34 Hz, 1H), 8.32 (br. s, 1H), 8.12 (br. s, 1H), 7.85-8.04 (m, 4H), 7.28-7.44 (m, 3H), 7.14-7.21 (m, 1H), 6.83 (br. s, 1H), 5.38 (br. s, 1H); LCMS (m/z): 347.2 [M+H]⁺.

Example 213 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(2,4-dimethylphenyl)amino]furan-3(2H)-one

The titled compound (solid) was similarly prepared according to the procedure described in the Example 210, using the compound of Example 26.

¹H NMR (DMSO-d₆) δ (ppm) 12.22 (br. s, 1H), 10.21 (br. s, 1H), 8.11-8.34 (m, 2H), 7.94 (br. s, 1H), 7.25 (d, J=7.92 Hz, 1H), 7.17 (br. s, 1H), 6.99-7.14 (m, 2H), 6.74 (s, 1H), 4.78 (br. s, 1H), 2.31 (s, 3H), 2.22 (s, 3H); LCMS (m/z): 332.2 [M+H]⁺.

Example 214 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-(p-tolylamino)furan-3(2H)-one

The titled compound (solid) was similarly prepared according to the procedure described in the Example 210, using the compound of Example 20.

¹H NMR (DMSO-d₆) δ (ppm) 12.32 (br. s, 1H), 8.40 (d, J=7.34 Hz, 1H), 8.30 (br. s, 1H), 8.06 (br. s, 1H), 7.10-7.36 (m, 5H), 6.74 (br. s, 1H), 5.12 (br. s, 1H), 2.32 (s, 3H); LCMS (m/z): 318.4 [M+H]⁺.

Example 215 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(4-bromophenyl)amino]furan-3(2H)-one

The titled compound (solid) was similarly prepared according to the procedure described in the Example 210, using the compound of Example 19.

¹H NMR (DMSO-d₆) δ (ppm) 12.31 (br. s, 1H), 8.39 (d, J=7.76 Hz, 1H), 8.23-8.35 (m, 1H), 8.05 (s, 1H), 7.58 (d, J=8.31 Hz, 2H), 7.27 (d, J=8.31 Hz, 2H), 7.18 (dd, J=4.65, 7.58 Hz, 1H), 6.80 (s, 1H), 5.20 (br. s, 1H); LCMS (m/z): 382.5 [M+H]⁺.

Example 216 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(3,4-dimethoxyphenyl)amino]furan-3(2H)-one

A solution of the compound (0.043 g, 0.099 mmol) of Example 28 in N,N-dimethylformamide (1.2 mL) was refluxed for 6 h. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC to afford the titled compound (0.0050 g, y. 12%).

¹H NMR (DMSO-d₆) δ (ppm) 12.35 (br. s, 1H), 10.56 (br. s, 1H), 8.40 (d, J=7.34 Hz, 1H), 8.30 (d, J=3.91 Hz, 1H), 8.06 (br. s, 1H), 7.08-7.22 (m, 1H), 7.01 (d, J=8.31 Hz, 1H), 6.92 (br. s, 1H), 6.87 (d, J=8.31 Hz, 1H), 6.77 (s, 1H), 5.12 (s, 1H), 3.78 (s, 6H); LCMS (m/z): 364.0 [M+H]⁺.

Example 217 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(4-fluorophenyl)amino]furan-3(2H)-one

The titled compound (solid) was similarly prepared according to the procedure described in the Example 216, using the compound of Example 21.

¹H NMR (DMSO-d₆) δ (ppm) 12.34 (br. s, 1H), 10.72 (br. s, 1H), 8.40 (d, J=7.34 Hz, 1H), 8.31 (d, J=3.91 Hz, 1H), 8.06 (br. s, 1H), 7.33-7.46 (m, 2H), 7.22-7.33 (m, 2H), 7.16 (dd, J=4.65, 7.58 Hz, 1H), 6.80 (s, 1H), 5.18 (s, 1H); LCMS (m/z): 322.0 [M+H]⁺.

Example 218 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(1,1′-biphenyl)-4-ylamino]furan-3(2H)-one

The titled compound (solid) was similarly prepared according to the procedure described in the Example 216, using the compound of Example 39.

¹H NMR (DMSO-d₆) δ (ppm) 12.29 (br. s, 1H), 8.24-8.49 (m, 3H), 8.07 (br. s, 1H), 7.70 (br. s, 3H), 7.48 (br. s, 2H), 7.38 (br. s, 3H), 7.15 (br. s, 1H), 6.72 (br. s, 1H), 5.19 (br. s, 1H); LCMS (m/z): 380.2 [M+H]⁺.

Example 219 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-{[2-(2-hydroxyethoxy)phenyl]amino}furan-3(2H)-one

A solution of the compound (0.015 g, 0.034 mmol) of Example 29 in N,N-dimethylformamide (0.50 mL) was refluxed for 4 h. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC to afford the titled compound as solid (0.0013 g, y. 11%).

¹H NMR (DMSO-d₆) δ (ppm) 12.24 (br. s, 1H), 8.44 (br. s, 1H), 8.24-8.35 (m, 2H), 8.03 (br. s, 1H), 7.32-7.41 (m, 1H), 6.96-7.25 (m, 4H), 6.67 (br. s, 1H), 4.90 (br. s, 1H), 4.01-4.12 (m, 2H), 3.67-3.80 (m, 2H); LCMS (m/z): 363.9 [M+H]⁺.

Example 220 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-(6-quinolinylamino)furan-3(2H)-one

A solution of the compound (0.075 g, 0.18 mmol) of Example 44 in N,N-dimethylformamide (4.0 mL) was refluxed for 6 h. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC to afford the titled compound as solid (0.0080 g, y. 13%).

¹H NMR (DMSO-d₆) δ (ppm) 12.41 (br. s, 1H), 11.07 (br. s, 1H), 8.81-8.87 (m, 1H), 8.42 (t, J=8.56 Hz, 2H), 8.31 (d, J=3.91 Hz, 1H), 8.15 (br. s, 1H), 8.07 (d, J=8.80 Hz, 1H), 7.88 (br. s, 1H), 7.73 (d, J=8.80 Hz, 1H), 7.55 (dd, J=3.91, 7.82 Hz, 1H), 7.15 (d, J=4.40 Hz, 1H), 6.85 (br. s, 1H), 5.56 (br. s, 1H); LCMS (m/z): 355.2 [M+H]⁺.

Example 221 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(2-methoxyphenyl)amino]furan-3(2H)-one

A solution of the compound (0.099 g, 0.24 mmol) of Example 7 in N,N-dimethylformamide (2.0 mL) was refluxed for 9 h. Cooled to ambient temperature, the reaction mixture was diluted with 2-butanol and washed with 4M sodium hydroxide solution. The organic layer was concentrated then purified by chromatography on silica gel(chloroform/methanol) to afford the titled compound as solid (0.0097 g, y. 12%).

¹H NMR (DMSO-d₆) δ (ppm) 12.31 (br. s, 1H), 10.29 (s, 1H), 8.33 (d, J=7.78 Hz, 1H), 8.27-8.30 (m, 1H), 8.12 (s, 1H), 7.42 (d, J=7.78 Hz, 1H), 7.26-7.33 (m, 1H), 7.18 (d, J=7.28 Hz, 1H), 7.10 (dd, J=4.77, 8.03 Hz, 1H), 7.04 (t, J=7.65 Hz, 1H), 6.75 (s, 1H), 4.90-4.94 (m, 1H), 3.88 (s, 3H); LCMS (m/z): 333.9 [M+H]⁺.

Example 222 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(2,4-difluorophenyl)amino]furan-3(2H)-one

A solution of the compound (0.099 g, 0.24 mmol) of Example 53 in N,N-dimethylformamide (1.0 mL) was refluxed for 4 h. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC to afford the titled compound as solid (0.0030 g, y. 4%).

¹H NMR (DMSO-d₆) δ (ppm) 12.31 (br. s, 1H), 10.60 (br. s, 1H), 8.26-8.36 (m, 2H), 8.01 (br. s, 1H), 7.55-7.65 (m, 1H), 7.44-7.54 (m, 1H), 7.16-7.25 (m, 1H), 7.06-7.14 (m, 1H), 6.79 (br. s, 1H), 4.95 (br. s, 1H); LCMS (m/z): 339.8 [M+H]⁺.

Example 223 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(4-morpholinophenyl)amino]furan-3(2H)-one

A solution of the compound (0.080 g, 0.17 mmol) of Example 42 in N,N-dimethylacetamide (3.0 mL) was stirred at 160° C. for 4 h. Cooled to ambient temperature, the reaction mixture was diluted with water and the precipitate was collected by filtration. The solid was washed with water, tetrahydrofuran and diethyl ether then dried to afford the titled compound as solid (0.015 g, y. 22%).

¹H NMR (DMSO-d₆) δ (ppm) 12.32 (br. s, 1H), 10.53 (br. s, 1H), 8.41 (d, J=8.12 Hz, 1H), 8.31 (d, J=4.28 Hz, 1H), 8.05 (br. s, 1H), 7.23 (d, J=8.31 Hz, 2H), 7.12-7.18 (m, 1H), 7.02 (d, J=8.31 Hz, 2H), 6.75 (s, 1H), 5.05 (s, 1H), 3.70-3.83 (m, 4H), 3.05-3.18 (m, 4H); LCMS (m/z): 389.4 [M+H]⁺.

Example 224 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(4-fluorobenzyl)amino]furan-3(2H)-one

A solution of the compound (0.10 g, 0.31 mmol) of Example 45 in N,N-dimethylacetamide (3.0 mL) was refluxed for 7 h. Cooled to ambient temperature, the reaction mixture was diluted with water and the precipitate was collected by filtration. The solid was washed with water and diethyl ether then dried to afford the titled compound as solid (0.0070 g, y. 9%).

¹H NMR (DMSO-d₆) δ (ppm) 12.23 (br. s, 1H), 9.08 (s, 1H), 8.40 (br. s, 1H), 8.21-8.32 (m, 2H), 7.96 (br. s, 1H), 7.34-7.50 (m, 1H), 7.05-7.32 (m, 3H), 6.67 (s, 1H), 4.91 (s, 1H), 4.35-4.60 (m, 2H); LCMS (m/z): 336.0 [M+H]⁺.

Example 225 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(2-thienylmethyl)amino]furan-3(2H)-one

A solution of the compound (0.10 g, 0.26 mmol) of Example 60 in N,N-dimethylformamide (0.80 mL) was refluxed for 16 h. Cooled to ambient temperature, the reaction mixture was diluted with chloroform and the precipitate was collected by filtration. The solid was washed with chloroform/methanol (10/1) and hexane then dried to afford the titled compound as solid (0.019 g, y. 22%).

¹H NMR (DMSO-d₆) δ (ppm) 12.25 (br. s, 1H), 9.20 (t, J=6.02 Hz, 1H), 8.41 (d, J=7.53 Hz, 1H), 8.29 (dd, J=1.26, 4.52 Hz, 1H), 7.99 (d, J=2.01 Hz, 1H), 7.49 (dd, J=1.00, 5.02 Hz, 1H), 7.12-7.20 (m, 2H), 7.02 (dd, J=3.51, 5.02 Hz, 1H), 6.67 (s, 1H), 4.96 (br. s, 1H), 4.69 (br. s, 2H); LCMS (m/z): 324.0 [M+H]⁺.

Example 226 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-(cycloheptylamino)furan-3(2H)-one

A solution of the compound (0.040 g, 0.10 mmol) of Example 59 in N,N-dimethylformamide (2.0 mL) was refluxed for 8 h. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC to afford the titled compound as solid (0.0040 g, y. 12%).

¹H NMR (DMSO-d₆) δ (ppm) 12.26 (br. s, 1H), 8.64 (d, J=7.82 Hz, 1H), 8.39 (d, J=7.34 Hz, 1H), 8.29 (d, J=3.91 Hz, 1H), 8.00 (br. s, 1H), 7.17 (dd, J=4.89, 7.82 Hz, 1H), 6.61 (s, 1H), 4.78 (br. s, 1H), 2.83-3.11 (m, 1H), 1.85-2.02 (m, 2H), 1.40-1.78 (m, 10H); LCMS (m/z): 324.2 [M+H]⁺.

Example 227 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-(isopropylamino)furan-3(2H)-one

A solution of the compound (0.15 g, 0.43 mmol) of Example 56 in N,N-dimethylacetamide (1.5 mL) was refluxed for 12 h. Cooled to ambient temperature, the reaction mixture was diluted with water and the precipitate was collected by filtration. The solid was washed with diethyl ether then dried to afford the titled compound as solid (0.015 g, y. 12%).

¹H NMR (DMSO-d₆) δ (ppm) 12.24 (br. s, 1H), 8.58 (d, J=7.82 Hz, 1H), 8.38 (d, J=7.83 Hz, 1H), 8.27 (d, J=4.40 Hz, 1H), 7.97 (br. s, 1H), 7.15 (dd, J=4.89, 7.82 Hz, 1H), 6.60 (s, 1H), 4.79 (br. s, 1H), 3.53-3.69 (m, 1H), 1.22 (d, J=6.36 Hz, 6H); LCMS (m/z): 270.2 [M+H]⁺.

Example 228 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(3-pyrazolyl)amino]furan-3(2H)-one

The titled compound (solid) was similarly prepared according to the procedure described in the Example 227, using the compound of Example 67.

¹H NMR (DMSO-d₆) δ (ppm) 12.52 (br. s, 2H), 11.67 (br. s, 1H), 8.45 (d, J=6.85 Hz, 1H), 8.16 (d, J=3.42 Hz, 1H), 7.72 (br. s, 2H), 7.56 (br. s, 1H), 7.13-7.25 (m, 1H), 6.98-7.09 (m, 1H), 4.69 (s, 1H); LCMS (m/z): 294.2 [M+H]⁺.

Example 229 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(3-fluorobenzyl)amino]furan-3(2H)-one

A solution of the compound (0.050 g, 0.12 mmol) of the Example 58 in N,N-dimethyacetamide (1.5 mL) was refluxed for 12 h. Cooled to ambient temperature, the reaction mixture was diluted with water and the precipitate was collected by filtration. The solid was purified by preparative HPLC to afford the titled compound as solid (0.012 g, y. 30%).

¹H NMR (DMSO-d₆) δ (ppm) 12.26 (br. s, 1H), 9.13-9.23 (m, 1H), 8.41 (br. s, 1H), 8.29 (d, J=3.91 Hz, 1H), 7.98 (br. s, 1H), 7.38-7.53 (m, 1H), 7.20-7.32 (m, 2H), 7.16 (d, J=6.36 Hz, 2H), 6.66 (s, 1H), 4.91 (br. s, 1H), 4.41-4.60 (m, 2H); LCMS (m/z): 336.0 [M+H]⁺.

Example 230 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-(cyclopropylamino)furan-3(2H)-one

To a solution of the compound (0.25 g, 0.76 mmol) of Example 94 in ethanol (7.0 mL), 50% potassium hydroxide solution (4.0 mL, 0.071 mol) was added at ambient temperature. The mixture was refluxed for 2 h. Cooled to ambient temperature, the solvent was removed under reduced pressure. The residue was diluted with water, 1M hydrochloric acid was added to adjust pH to acidic, and then the solvent was removed under reduced pressure. The residue was purified by preparative HPLC to afford the titled compound as solid (0.0090 g, y. 4%).

¹H NMR (DMSO-d₆) δ (ppm) 12.26 (br. s, 1H), 8.88 (br. s, 1H), 8.44 (d, J=6.85 Hz, 1H), 8.22-8.34 (m, 1H), 7.97 (br. s, 1H), 7.12-7.20 (m, 1H), 6.66 (br. s, 1H), 4.87 (br. s, 1H), 2.62-2.80 (m, 1H), 0.72-0.88 (m, 2H), 0.55-0.72 (m, 2H); LCMS (m/z): 268.0 [M+H]⁺.

Example 231 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(2-methoxyethyl)amino]furan-3(2H)-one

To a solution of the compound (0.070 g, 0.20 mmol) of Example 98 in ethanol (2.0 mL), 50% potassium hydroxide solution (1.0 mL, 0.018 mol) was added at ambient temperature. The mixture was refluxed for 1 h. Cooled to ambient temperature, 1M hydrochloric acid was added to adjust pH to acidic, and then the solvent was removed under reduced pressure. To this residue, ethanol (2.0 mL) and water (2.0 mL) were added then refluxed for 1 h. Cooled to ambient temperature, the solvent was removed under reduced pressure, and the residue was purified by preparative HPLC to afford the titled compound as solid (0.0060 g, y. 12%).

¹H NMR (DMSO-d₆) δ (ppm) 12.24 (br. s, 1H), 8.65-8.90 (m, 1H), 8.41 (d, J=6.85 Hz, 1H), 8.29 (br. s, 1H), 8.01 (br. s, 1H), 7.08-7.32 (m, 1H), 6.53-6.70 (m, 1H), 4.72-4.97 (m, 1H), 3.30-3.55 (m, 4H), 3.26 (br. s, 3H); LCMS (m/z): 286.2 [M+H]⁺.

Example 232 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(1-methyl-4-piperidinyl)amino]furan-3(2H)-one

The titled compound (solid) was similarly prepared according to the procedure described in the Example 231, using the compound of Example 65.

¹H NMR (DMSO-d₆) δ (ppm) 12.25 (br. s, 1H), 8.61 (d, J=7.34 Hz, 1H), 8.39 (d, J=7.82 Hz, 1H), 8.23-8.34 (m, 1H), 8.17 (br. s, 1H), 7.99 (br. s, 1H), 7.06-7.24 (m, 1H), 6.62 (s, 1H), 4.77-4.99 (m, 1H), 2.73-2.85 (m, 2H), 2.22 (s, 3H), 2.00-2.14 (m, 2H), 1.82-1.97 (m, 2H), 1.49-1.67 (m, 2H); LCMS (m/z): 325.4 [M+H]⁺.

Example 233 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(4-fluoro-2-methylphenyl)amino]furan-3(2H)-one

To a solution of the compound (0.15 g, 0.37 mmol) of Example 97 in ethanol (4.0 mL), 50% potassium hydroxide solution (2.0 mL, 0.036 mol) was added at ambient temperature. The mixture was refluxed for 2 h. Cooled to ambient temperature, the solvent was removed under reduced pressure. Water was added to this residue, the mixture was washed with ethyl acetate then 1M hydrochloric acid was added to adjust pH to acidic. The precipitate was collected by filtration, washed with water, diethyl ether, ethyl acetate and hexane then dried to afford the titled compound as solid (0.12 g, y. 93%).

¹H NMR (DMSO-d₆) δ (ppm) 12.28 (br. s, 1H), 10.24 (s, 1H), 8.19-8.32 (m, 2H), 7.97 (br. s, 1H), 7.43 (dd, J=5.62, 8.56 Hz, 1H), 7.26 (d, J=7.34 Hz, 1H), 7.11-7.19 (m, 1H), 7.04-7.10 (m, 1H), 6.74 (s, 1H), 4.72 (s, 1H), 2.31 (s, 3H); LCMS (m/z): 335.8 [M+H]⁺.

Example 234 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(2,6-dimethyl-3-pyridinyl)amino]furan-3(2H)-one

To a solution of the compound (0.090 g, 0.20 mmol) of Example 107 in ethanol (4.0 mL), 50% potassium hydroxide solution (2.0 mL, 0.036 mol) was added at ambient temperature. The mixture was refluxed for 2 h. Cooled to ambient temperature, the solvent was removed under reduced pressure. Water was added to this residue, the mixture was washed with ethyl acetate then 1M hydrochloric acid was added to adjust pH to acidic. The precipitate was collected by filtration, washed with water and diethyl ether then dried to afford the titled compound (0.037 g, y. 50%) as solid.

¹H NMR (DMSO-d₆) δ (ppm) 12.26 (br. s, 1H), 10.33 (br. s, 1H), 8.28 (d, J=4.40 Hz, 2H), 7.96 (br. s, 1H), 7.64 (d, J=8.31 Hz, 1H), 7.17 (d, J=7.82 Hz, 1H), 7.02-7.12 (m, 1H), 6.70 (br. s, 1H), 4.73 (br. s, 1H), 2.48 (br. s, 3H), 2.44 (br. s, 3H); LCMS (m/z): 333.3 [M+H]⁺.

Example 235 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(2-fluorobenzyl)amino]furan-3(2H)-one

To a solution of the compound (0.10 g, 0.25 mmol) of Example 101 in ethanol (4.0 mL), 50% potassium hydroxide solution (2.0 mL, 0.036 mol) was added at ambient temperature. The mixture was refluxed for 2 h. Cooled to ambient temperature, the solvent was removed under reduced pressure. Water was added to this residue, the mixture was washed with ethyl acetate then 1M hydrochloric acid was added to adjust pH to acidic, and then extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrate then purified by chromatography on silica gel(dichloromethane/methanol) to afford the titled compound as solid (0.012 g, y. 14%).

¹H NMR (DMSO-d₆) δ (ppm) 12.24 (br. s, 1H), 9.08-9.17 (m, 1H), 8.34-8.46 (m, 1H), 8.22-8.33 (m, 1H), 7.98 (br. s, 1H), 7.45-7.55 (m, 1H), 7.34-7.43 (m, 1H), 7.20-7.31 (m, 2H), 7.10-7.19 (m, 1H), 6.65 (s, 1H), 4.83-4.97 (m, 1H), 4.40-4.64 (m, 2H); LCMS (m/z): 335.8 [M+H]⁺.

Example 236 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(2-phenyl-2-propanyl)amino]furan-3(2H)-one

To a solution of the compound (0.060 g, 0.14 mmol) of Example 112 in ethanol (2.0 mL), 50% potassium hydroxide solution (1.0 mL, 0.018 mol) was added at ambient temperature. The mixture was refluxed for 2 h. Cooled to ambient temperature, the solvent was removed under reduced pressure. Water was added to this residue, the mixture was washed with ethyl acetate then 1M hydrochloric acid was added to adjust pH to acidic. The precipitate was collected by filtration, washed with water and diethyl ether then dried to afford the titled compound as solid (0.010 g, y. 20%).

¹H NMR (DMSO-d₆) δ (ppm) 12.26 (br. s, 1H), 9.05 (br. s, 1H), 8.24-8.38 (m, 2H), 8.0 (br, 1H), 7.36-7.50 (m, 4H), 7.25-7.32 (m, 1H), 7.12-7.18 (m, 1H), 6.57 (s, 1H), 4.06 (br. s, 1H), 1.70 (s, 6H); LCMS (m/z): 346.2 [M+H]⁺.

Example 237 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-(2-adamantylamino)furan-3(2H)-one

The titled compound (solid) was similarly prepared according to the procedure described in the Example 236, using the compound of Example 113.

¹H NMR (DMSO-d₆) δ (ppm) 12.29 (br. s, 1H), 8.55 (br. s, 1H), 8.37 (d, J=7.82 Hz, 1H), 8.30 (d, J=4.40 Hz, 1H), 8.14 (br. s, 1H), 7.17 (dd, J=4.89, 7.83 Hz, 1H), 6.64 (s, 1H), 4.83 (s, 1H), 3.25-3.40 (m, 1H), 1.96-2.11 (m, 4H), 1.80-1.92 (m, 4H), 1.69-1.77 (m, 2H), 1.55-1.63 (m, 2H), 1.09 (t, J=7.00 Hz, 2H); LCMS (m/z): 362.4 [M+H]⁺.

Example 238 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-(cyclopentylamino)furan-3(2H)-one

To a solution of the compound (0.10 g, 0.27 mmol) of Example 103 in ethanol (4.0 mL), 50% potassium hydroxide solution (2.0 mL, 0.036 mol) was added at ambient temperature. The mixture was refluxed for 2 h. Cooled to ambient temperature, the solvent was removed under reduced pressure. Water was added to this residue, the mixture was washed with ethyl acetate then 1M hydrochloric acid was added to adjust pH to acidic, and then the solvent was removed under reduced pressure. The residue was purified by preparative HPLC to afford the titled compound as solid (0.0015 g, y. 5%).

¹H NMR (DMSO-d₆) δ (ppm) 12.25 (br. s, 1H), 8.58-8.70 (m, 1H), 8.39 (d, J=7.83 Hz, 1H), 8.22-8.32 (m, 1H), 7.99 (br. s, 1H), 7.17 (dd, J=4.65, 7.58 Hz, 1H), 6.62 (s, 1H), 6.55 (s, 1H), 4.75-4.96 (m, 1H), 1.90-2.03 (m, 2H), 1.65-1.77 (m, 2H), 1.50-1.65 (m, 4H); LCMS (m/z): 296.2 [M+H]⁺.

Example 239 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(5-indazolyl)amino]furan-3(2H)-one

To a solution of the compound (0.14 g, 0.33 mmol) of Example 118 in ethanol (4.0 mL), 50% potassium hydroxide solution (2.2 mL, 0.039 mol) was added at ambient temperature. The mixture was refluxed for 2 h. Cooled to ambient temperature, 1M hydrochloric acid was added to adjust pH to acidic, and the solvent was removed under reduced pressure. The residue was purified by preparative HPLC to afford the titled compound as solid (0.010 g, y. 9%).

¹H NMR (DMSO-d₆) δ (ppm) 13.16 (br. s, 1H), 12.30 (br. s, 1H), 8.42 (br. s, 1H), 8.37 (d, J=7.76 Hz, 1H), 8.28 (d, J=3.91 Hz, 1H), 8.09 (br. s, 1H), 8.05 (br. s, 1H), 7.71 (s, 1H), 7.61 (d, J=8.80 Hz, 1H), 7.35 (d, J=8.80 Hz, 1H), 7.04-7.11 (m, 1H), 6.73 (s, 1H), 5.10 (s, 1H); LCMS (m/z): 344.2 [M+H]⁺.

Example 240 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(5-benzimidazolyl)amino]furan-3(2H)-one

To a solution of the compound (0.055 g, 0.13 mmol) of Example 119 in ethanol (6.0 mL), 50% potassium hydroxide solution (3.0 mL, 0.053 mol) was added at ambient temperature. The mixture was refluxed for 1.5 h. Cooled to ambient temperature, 1M hydrochloric acid was added to adjust pH to acidic, and the solvent was removed under reduced pressure. The residue was purified by preparative HPLC to afford the titled compound (0.0090 g, y. 20%) as solid.

¹H NMR (DMSO-d₆) δ (ppm) 12.28 (br. s, 1H), 12.10 (br. s, 1H), 8.34 (br. s, 1H), 8.22-8.29 (m, 1H), 8.10-8.20 (m, 1H), 7.95 (br. s, 1H), 7.50-7.62 (m, 1H), 7.25-7.38 (m, 1H), 6.98-7.15 (m, 2H), 6.34-6.67 (m, 2H), 4.84 (s, 1H); LCMS (m/z): 344.0 [M+H]⁺.

Example 241 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(1-phenylethyl)amino]furan-3(2H)-one

The titled compound (solid) was similarly prepared according to the procedure described in the Example 240, using the compound of Example 106.

¹H NMR (DMSO-d₆) δ (ppm) 12.29 (br. s, 1H), 9.15 (d, J=7.83 Hz, 1H), 8.23-8.43 (m, 2H), 7.80 (br. s, 1H), 7.11-7.49 (m, 6H), 6.62 (s, 1H), 5.00-5.10 (m, 1H), 4.60-4.80 (m, 1H), 1.36-1.60 (m, 3H); LCMS (m/z): 332.2 [M+H]⁺.

Example 242 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-{[(3-methyl-2-thienyl)methyl]amino}furan-3(2H)-one

A solution of the compound (0.050 g, 0.12 mmol) of Example 124 in N,N-dimethylformamide (1.0 mL) was refluxed for 16 h. Cooled to ambient temperature, the reaction mixture was purified by preparative HPLC to afford the titled compound as solid (0.012 g, y. 28%).

¹H NMR (DMSO-d₆) δ (ppm) 12.25 (br. s, 1H), 9.09 (t, J=5.77 Hz, 1H), 8.40 (d, J=7.78 Hz, 1H), 8.29 (dd, J=1.38, 4.64 Hz, 1H), 7.99 (d, J=2.26 Hz, 1H), 7.38 (d, J=5.02 Hz, 1H), 7.15 (dd, J=4.77, 8.03 Hz, 1H), 6.90 (d, J=5.02 Hz, 1H), 6.66 (s, 1H), 4.92 (br. s, 1H), 4.59 (br. s, 2H), 2.25 (s, 3H); LCMS (m/z): 338.1 [M+H]⁺.

Example 243 2-[(1H-Pyrrolo[2,3-b]pyridin-3-yl)methylene]-5-[(trans-4-hydroxycyclohexyl)amino]furan-3(2H)-one

The titled compound (solid) was similarly prepared according to the procedure described in the Example 240, using the compound of Example 116.

¹H NMR (DMSO-d₆) δ (ppm) 12.23 (br. s, 1H), 8.51-8.60 (m, 1H), 8.34-8.43 (m, 1H), 8.24-8.32 (m, 1H), 7.92-8.10 (m., 1H), 7.16 (dd, J=4.64, 7.91 Hz, 1H), 6.61 (s, 1H), 4.85 (br. s, 1H), 4.40-4.72 (m, 2H), 1.69-2.07 (m, 4H), 1.02-1.54 (m, 4H); LCMS (m/z): 326.1 [M+H]⁺.

Example 244 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(7-methoxy-3,4-dihydroisoquinolin-2(1H)-yl)-4-oxo-4,5-dihydrofuran-3-carboxylate

First Step

To a suspension of 7-methoxy-1,2,3,4-tetrahydroisoquinoline hydrochloride (2.10 g, 10.5 mmol) in tetrahydrofuran, triethylamine (3.48 mL, 25.0 mmol) was added at ambient temperature and the mixture was stirred for 30 min. To this reaction mixture, ethyl 2-ethoxy-4-oxo-4,5-dihydrofuran-3-carboxylate (2.00 g, 10.0 mmol), which afforded in the Example 74, Third step was added and the mixture was stirred for 5 days. Water was added to the reaction mixture, and the mixture was extracted with ethyl acetate. The organic layer was washed with brine, dried over sodium sulfate and concentrated. The residue was purified by chromatography on silica gel(chloroform/methanol) to afford ethyl 2-[7-methoxy-3,4-dihydroisoquinolin-2(1H)-yl]-4-oxo-4,5-dihydrofuran-3-carboxylate as oil (2.85 g, y. 89%).

¹H NMR (CDCl₃) δ (ppm) 7.09 (d, J=8.4 Hz, 1H), 6.80 (dd, J=8.4, 2.6 Hz, 1H), 6.64 (d, J=2.4 Hz, 1H), 4.78 (s, 2H), 4.54 (s, 2H), 4.33 (q, J=7.1 Hz, 2H), 3.89 (t, J=5.9 Hz, 2H), 3.79 (d, J=2.9 Hz, 3H), 2.86-3.04 (m, 2H), 1.38 (t, J=7.1 Hz, 3H); LCMS (m/z): 318.2 [M+H]⁺.

Second Step

To a solution of ethyl 2-[7-methoxy-3,4-dihydroisoquinolin-2(1H)-yl]-4-oxo-4,5-dihydrofuran-3-carboxylate (2.58 g, 8.98 mmol) and 7-azaindole-3-carboxaldehyde (1.31 g, 8.98 mmol) in ethanol (12 mL), L-proline was added at ambient temperature. The mixture was refluxed for 24 h. The precipitate was collected by filtration, washed with ethanol and diisopropyl ether then dried to afford the titled compound as solid (1.20 g, y. 30%).

¹H NMR (DMSO-d₆) δ (ppm) 12.37 (s, 1H), 8.41 (dd, J=7.9, 1.5 Hz, 1H), 8.33 (dd, J=4.7, 1.5 Hz, 1H), 8.08 (s, 1H), 7.26-7.13 (m, 2H), 6.90 (s, 2H), 6.85 (dd, J=8.3, 2.6 Hz, 1H), 4.95 (br. s, 2H), 4.21 (q, J=7.1 Hz, 2H), 3.85-4.05 (m, 2H), 3.76 (s, 3H), 3.00 (t, J=5.8 Hz, 2H), 1.26 (t, J=7.1 Hz, 3H); LCMS (m/z): 446.2 [M+H]⁺.

Example 245 Isopropyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-2-(morpholinoamino)-4-oxo-4,5-dihydrofuran-3-carboxylate

First Step

To a solution of isopropyl 2-isopropoxy-4-oxo-4,5-dihydrofuran-3-carboxylate (12.2 g, 53.6 mmol), which similarly prepared according to the procedure described in the Example 74, Third step using diisopropyl malonate and chloroacetyl chloride, in ethanol (45 mL), 4-aminomorpholine (5.7 mL, 59.2 mmol) was added at ambient temperature. The mixture was stirred for 18 h. The solvent was removed under reduced pressure, and the crude product was suspended in methyl tert-butyl ether, then the precipitate was collected by filtration, washed with methyl tert-butyl ether and hexane then dried to afford isopropyl 2-(morpholinoamino)-4-oxo-4,5-dihydrofuran-3-carboxylate as solid (11.2 g, y. 75%).

¹H NMR (DMSO-d₆) δ (ppm) 9.60 (s, 1H), 4.95-5.05 (m, 1H), 4.57 (s, 2H), 3.60-3.68 (m, 4H), 2.87-2.95 (m, 4H), 1.22 (d, J=6.40 Hz, 6H); LCMS (m/z): 271.0 [M+H]⁺

Second Step

To a stirred solution of isopropyl 2-(morpholinoamino)-4-oxo-4,5-dihydrofuran-3-carboxylate (5.00 g, 18.5 mmol) and 7-azaindole-3-carboxaldehyde (2.49 g, 17.1 mmol) in ethanol (25 mL), L-proline (0.24 g, 2.04 mmol) was added at ambient temperature. The mixture was refluxed for 3 days. The precipitate was collected by filtration, washed with hot ethanol then hexane, and then dried to afford the titled compound as solid (4.22 g, y. 62%).

¹H NMR (DMSO-d₆) δ (ppm) 12.34 (br. s, 1H), 10.07 (s, 1H), 8.94 (d, J=7.78 Hz, 1H), 8.33 (dd, J=1.38, 4.64 Hz, 1H), 8.12 (d, J=2.76 Hz, 1H), 7.22 (dd, J=4.52, 8.03 Hz, 1H), 6.84 (s, 1H), 4.99-5.12 (m, 1H), 3.75-3.86 (m, 4H), 3.03-3.13 (m, 4H), 1.27 (d, J=6.27 Hz, 6H); LCMS (m/z): 399.1 [M+H]⁺.

Example 246 Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin-3-yl)methylene]-4-oxo-2-{[4-(2,2,2-trifluoroethyl)piperazinyl]amino}-4,5-dihydrofuran-3-carboxylate

First Step

To a solution of 1-(2,2,2-trifluoroethyl)piperazine (4.88 g, 29.0 mmol) in water (4.9 mL) that cooled with ice bath, a solution of sodium nitrite (4.04 g, 58.6 mmol) in water (20 mL) and acetic acid (5.0 mL, 87.0 mmol) were added dropwise slowly, then the mixture was stirred for 1 h. The reaction mixture was all owed to warm to ambient temperature, stirred for further 2 h. The reaction mixture was adjusted to pH9 using sodium carbonate, diluted with ethyl acetate and washed with water and brine. The organic layer was dried over magnesium sulfate and concentrated to afford 1-nitrso-4-(2,2,2-trifluoroethyl)piperazine as oil (5.57 g, y. 97%).

¹H NMR (DMSO-d₆) δ (ppm) 4.18-4.22 (m, 2H), 3.72-3.76 (m, 2H), 3.32 (q, J=10.13 Hz, 2H), 2.84-2.89 (m, 2H), 2.59-2.64 (m, 2H).

Second Step

To a suspension of lithium aluminum hydride (2.22 g, 58.5 mmol) in tetrahydrofuran (100 mL) that cooled with ice bath, a solution of 1-nitroso-4-(2,2,2-trifluoroethyl)piperazine (5.43 g, 27.5 mmol) in tetrahydrofuran (10 mL) was added dropwise and the reaction mixture was allowed to warm to ambient temperature, then stirred for 5 days. The reaction mixture was cooled with ice bath, ethyl acetate was added until cease bubbling up, and water (2.1 mL) was added then the mixture was stirred for 20 min. To this mixture, 15% w/v sodium hydroxide solution (2.1 mL) and water (6.3 mL) were added, then the suspension mixture was allowed to warm to ambient temperature and then the mixture was stirred vigorously for 1 h. Magnesium sulfate was added to the mixture and stirred for a while, then the suspension was filtered. The filtrate was concentrated to afford 4-(2,2,2-trifluoroethyl)piperazine-1-amine as oil (4.72 g, y. 94%).

¹H NMR (DMSO-d₆) δ (ppm) 3.12 (br. s, 1H), 2.98 (q, J=9.6 Hz, 2H), 2.60-2.80 (m, 8H).

Third Step

To a solution of 4-(2,2,2-trifluoroethyl)piperazine-1-amine (4.72 g, 25.8 mmol) in ethanol (43 mL), ethyl 2-ethoxy-4-oxo-4,5-dihydrofuran-3-carboxylate (4.70 g, 23.5 mmol) which similarly prepared according to the procedure described in the Example 74, Third step was added at ambient temperature. The mixture was stirred for 2 h. The precipitate was collected by filtration, washed with diethyl ether and hexane then dried to afford ethyl 4-oxo-2-{[4-(2,2,2-trifluoroethyl)piperazinyl]amino}-4,5-dihydrofuran-3-carboxylate as solid (5.64 g, y. 71%).

¹H NMR (DMSO-d₆) δ (ppm) 8.94 (s, 1H), 4.61 (s, 2H), 4.32 (q, J=7.07 Hz, 2H), 3.03 (q, J=9.47 Hz, 2H), 2.95-2.99 (m, 4H), 2.85-2.90 (m, 4H), 1.37 (t, J=7.20 Hz, 3H); LCMS (m/z): 338.1 [M+H]⁺.

Fourth Step

To a solution of ethyl 4-oxo-2-{[4-(2,2,2-trifluoroethyl)piperazinyl]amino}-4,5-dihydrofuran-3-carboxylate (3.01 g, 8.93 mmol) and 7-azaindole-3-carboxaldehyde (1.25 g, 8.55 mmol) in ethanol (25 mL), L-proline (99 mg, 0.86 mmol) was added at ambient temperature then the mixture was refluxed for 2 days. The precipitate was collected by filtration, washed with hot ethanol then hexane, and then dried to afford the titled compound a solid (2.64 g, y. 66%).

¹H NMR (DMSO-d₆) δ (ppm) 12.32 (s, 1H), 10.09 (s, 1H), 8.95 (d, J=7.6 Hz, 1H), 8.33 (d, J=4.1 Hz, 1H), 8.09 (s, 1H), 7.30-7.16 (m, 1H), 6.80 (s, 1H), 4.19 (q, J=7.1 Hz, 2H), 3.31-3.41 (m, 2H), 3.06 (s, 4H), 2.88 (s, 4H), 1.25 (t, J=7.0 Hz, 3H); LCMS (m/z): 466.0 [M+H]⁺.

Each of the Example compounds shown in the following [Table 1-1] to [Table 1-10] were prepared according to the procedure described in the above Examples or modified procedure well known in the art of organic chemistry if needed, using appropriate starting materials (those materials which are commercially available, or which are derivatized by known methods or their modified methods from commercial compounds). The physicochemical data of each compound were shown in the following [Table 2-1] to [Table 2-6].

TABLE 1-1 Example Chemical Formula Chemical Name 247

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(1,4-diazepan-1- yl)-4-oxo-4,5-dihydrofuran-3- carboxylate 248

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(4,5- dihydrothieno[2,3-c]pyridin-6(7H)-yl)- 4-oxo-4,5-dihydrofuran-3-carboxylate 249

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(4,5-dihydro-1H- benzo[c]azepin-2(3H)-yl)-4-oxo- 4,5-dihydrofuran-3-carboxylate 250

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(2,3-dihydrobenzo- [f][1,4]oxazepin-4(5H)-yl)-4-oxo- 4,5-dihydrofuran-3-carboxylate 251

Carbamoylmethyl 5-[(1H-pyrrolo [2,3-b]pyridin-3-yl)methylene]-2- [(4-fluorophenyl)amino]-4-oxo- 4,5-dihydrofuran-3-carboxylate 252

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-[(1-hydroxypropan- 2-yl)amino]-4-oxo-4,5-dihydrofuran- 3-carboxylate 253

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-[(2,2-dimethyl- 1,3-dioxolan-4-yl)methylamino]-4- oxo-4,5-dihydrofuran-3-carboxylate 254

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-[(2-hydroxypropyl) amino]-4-oxo-4,5-dihydrofuran-3- carboxylate 255

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-4-oxo-2-(prop-2-yn- 1-ylamino)-4,5-dihydrofuran-3- carboxylate 256

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(2- benzoylhydrazinyl)-4-oxo-4,5- dihydrofuran-3-carboxylate 257

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(2,3-dihydrobenzo [f][1,4]thiazepin-4(5H)-yl)-4-oxo- 4,5-dihydrofuran-3-carboxylate 258

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(2,3-dihydro-1H- benzo[e][1,4]diazepin-4(5H)-yl)-4- oxo-4,5-dihydrofuran-3-carboxylate

TABLE 1-2 259

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(5-methoxy-3,4- dihydroisoquinolin-2(1H)-yl)-4-oxo- 4,5-dihydrofuran-3-carboxylate 260

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-[(2S,6R)-2,6- dimethylmorpholino]-4-oxo-4,5- dihydrofuran-3-carboxylate 261

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-4-oxo-2- [(tetrahydro-2H-pyran-4-yl)amino]- 4,5-dihydrofuran-3-carboxylate 262

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-4-oxo-2-(pyrrolidin- 1-ylamino)-4,5-dihydrofuran-3- carboxylate 263

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(azepan-1- 4-oxo-4,5-dihydrofuran-3- carboxylate 264

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-({4-[(4- isopropylpiperazinyl)methyl]-2- methylphenyl}amino)-4-oxo-4,5- dihydrofuran-3-carboxylate 265

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-({4- [(cyclopentylamino)methyl]-2- methylphenyl}amino-4-oxo-4,5- dihydrofuran-3-carboxylate 266

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-({4- [(isopropylamino)methyl]-2- methylphenyl}amino)-4-oxo-4,5- dihydrofuran-3-carboxylate 267

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-{[4-(4- hydroxypiperidinomethyl)-2- methylphenyl]amino}-4-oxo-4,5- dihydrofuran-3-carboxylate 268

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(cyanomethylamino)- 4-oxo-4,5-dihydrofuran-3-carboxylate 269

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-[(1,3- dihydroxypropan-2-yl)amino]-4- oxo-4,5-dihydrofuran-3-carboxylate 270

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-[(2,3-dihydroxypropyl) amino]-4-oxo-4,5-dihydrofuran- 3-carboxylate

TABLE 1-3 271

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(4-{[2- (dimethylamino)ethyl](N-methyl) amino}piperidino)-4-oxo-4,5- dihydrofuran-3-carboxylate 272

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(2-{[(2- methoxyethoxy)methoxy]methyl} piperidino)-4-oxo-4,5-dihydrofuran- 3-carboxylate 273

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(6-methoxy-3,4- dihydroisoquinolin-2(1H)-yl)-4-oxo- 4,5-dihydrofuran-3-carboxylate 274

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-({2-methyl-4- [(methylamino)methyl]phenyl} amino)-4-oxo-4,5-dihydrofuran-3- carboxylate 275

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(3- methylpiperidino)-4-oxo-4,5- dihydrofuran-3-car boxylate 276

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(2,6- dimethylmorpholino)-4-oxo-4,5- dihydrofuran-3-carboxylate 277

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-{[4-(2- hydroxyethyl)piperazinyl]amino}-4- oxo-4,5-dihydrofuran-3-carboxylate 278

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-[(3R,5S)-3,5- dimethylpiperidino]-4-oxo-4,5- dihydrofuran-3-carboxylate 279

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-[(4H-1,2,4- triazol-4-yl)amino]-4-oxo-4,5- dihydrofuran-3-carboxylate 280

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-[8-methoxy-3,4- dihydroisoquinolin-2(1H)-yl]-4-oxo- 4,5-dihydrofuran-3-carboxylate

TABLE1-4 281

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(2,6- dimethylpiperidinoamino)-4-oxo-4,5- dihydrofuran-3-carboxylate 282

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-[(3S,5S)-3,5- dimethylpiperidino]-4-oxo-4,5- dihydrofuran-3-carboxylate 283

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(3,3- dimethylpiperidino)-4-oxo-4,5- dihydrofuran-3-carboxylate 284

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-[2-(hydroxymethyl) piperidino]-4-oxo-4,5- dihydrofuran-3-carboxylate 285

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-{[2-methyl-4-(2- morpholinoethyl)phenyl]aminol-4- oxo-4,5-dihydrofuran-3-carboxylate 286

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-{[4-(2- hydroxyethyl)-2-methylphenyl]amino}- 4-oxo-4,5-dihydrofuran-3-carboxylate 287

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-({2-methy1-4- [3-(pyrrolidinyl)prop-1-yn-1-yl] phenyl}amino)-4-oxo-4,5-dihydrofuran- 3-carboxylate acetate 288

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-{[2-methyl-4- (3-morpholinoprop-1-yn-1-yl)phenyl] amino}-4-oxo-4,5-dihydrofuran-3- carboxylate formate 289

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-({4-[3-(4- hydroxypiperidino)prop-1-yn-1-yl]- 2-methylphenyl}amino)-4-oxo-4,5- dihydrofuran-3-carboxylate 290

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-({2-methyl-4- [2-(4-methylpiperazinyl)ethyl]phenyl} amino)-4-oxo-4,5-dihydrofuran-3- carboxylate 291

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-[ethyl(N-methyl) amino]-4-oxo-4,5-dihydrofuran-3- carboxylate

TABLE 1-5 292

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-[isopropyl(N- methyl)amino]-4-oxo-4,5- dihydrofuran-3-carboxylate 293

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(3- hydroxypiperidinoamino)-4-oxo- 4,5-dihydrofuran-3-carboxylate 294

Isopropyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2-(4- methylpiperidino)-4-oxo-4,5- dihydrofuran-3-carboxylate 295

Isopropyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2-(3,4- dihydroisoquinolin-2(1H)-yl)-4- oxo-4,5-dihydrofuran-3- carboxylate 296

Isopropyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2-(2,2- dimethylhydrazinyl)-4-oxo-4,5- dihydrofuran-3-carboxylate 297

Isopropyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-({4-[(2- hydroxyethyl)amino]-2-methylphenyl} amino)-4-oxo-4,5-dihydrofuran- 3-carboxylate 298

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-({2-methyl-4- [3-(4-methylpiperazinyl)prop-1-yn-1- yl]phenyl}amino)-4-oxo-4,5- dihydrofuran-3-carboxylate 299

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-({2-methyl-4- [2-(pyrrolidinyl)ethyl]phenyl}amino)- 4-oxo-4,5-dihydrofuran-3-carboxylate 300

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-[cyclohexyl(N- methyl)amino]-4-oxo-4,5- dihydrofuran-3-carboxylate 301

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(2-cyclohexyl-2- methylhydraziny1)-4-oxo-4,5- dihydrofuran-3-carboxylate 302

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-[(4- acetylpiperazinyl)amino]-4-oxo-4,5- dihydrofuran-3-carboxylate

TABLE 1-6 303

Isopropyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2-({2-methyl- 4-[2-(pyrrolidinyl)ethoxy]phenyl {amino)-4-oxo-4,5-dihydrofuran- 3-carboxylate 304

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(2-ethyl-2- methylhydrazinyl)-4-oxo-4,5- dihydrofuran-3-carboxylate 305

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(2,2- diethylhydraziny1)-4-oxo-4,5- dihydrofuran-3-carboxylate 306

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(4- hydroxypiperidinoamino)-4-oxo- 4,5-dihydrofuran-3-carboxylate 307

Isopropyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2-{[2-methyl- 4-(2-morpholinoethoxy)phenyl] aminol-4-oxo-4,5-dihydrofuran- 3-carboxylate 308

Isopropyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2-({2-methyl- 4-[2-(4-methylpiperazinyl)ethoxy] phenyl}amino)-4-oxo-4,5- dihydrofuran-3-carboxylate 309

2-Methoxyethyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2- (cyclohexylamino)-4-oxo-4,5- dihydrofuran-3-carboxylate 310

2-Methoxyethyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2-(2,2- dimethylhydrazinyl)-4-oxo-4,5- dihydrofuran-3-carboxylate 311

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-{[4-(3-hydroxyprop- 1-yn-1-yl)-2-methylphenyl]amino}- 4-oxo-4,5-dihydrofuran-3-carboxylate 312

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-({4-[3- (dimethylamino)prop-1-yn-1-yl]- 2-methylphenyl}amino)-4-oxo-4,5- dihydrofuran-3-carboxylate 313

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-({4-[2- (dimethylamino)ethyl]-2-methylphenyl} amino)-4-oxo-4,5-dihydrofuran-3- carboxylate

TABLE 1-7 314

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-({4-[2-(4- hydroxypiperidino)ethyl]-2- methylphenyl}amino)-4-oxo- 4,5-dihydrofuran-3-carboxylate 315

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(2-isopropyl-2- methylhydrazinyl)-4-oxo-4,5- dihydrofuran-3-carboxylate 316

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-4-oxo-2-[(3- oxopiperazinyl)amino]-4,5- dihydrofuran-3-carboxylate 317

2-Methoxyethyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2-(3,4- dihydroisoquinolin-2 (1H)-yl)-4-oxo- 4,5-dihydrofuran-3-carboxylate 318

2-Methoxyethyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2-({4- [(2-hydroxyethyl)amino]-2- methylphenyl}amino)-4-oxo-4,5- dihydrofuran-3-carboxylate 319

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-4-oxo-2-[4-(2,2,2- trifluoroethyl)piperazinyl]-4,5- dihydrofuran-3-carboxylate 320

Isopropyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2-({4- [(dimethylamino)methyl]-2- methylphenyl}amino)-4-oxo- 4,5-dihydrofuran-3- carboxylate acetate 321

2-Methoxyethyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2-(4- methylpiperidino)-4-oxo-4,5- dihydrofuran-3-carboxylate 322

2-Methoxyethyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2-{[2- methyl-4-(2-morpholinoethoxy)phenyl] amino}-4-oxo-4,5-dihydrofuran-3- carboxylate 323

2-Methoxyethyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2-({2- methy1-4-[2-(4-methylpiperazinyl) ethoxy]phenyl}amino)-4-oxo-4,5- dihydrofuran-3-carboxylate acetate 324

2-Methoxyethyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2-({4- [(dimethylamino)methyl]-2- methylphenyl}amino)-4-oxo-4,5- dihydrofuran-3-carboxylate 325

3-Hydroxypropyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2- (cyclohexylamino)-4-oxo-4,5- dihydrofuran-3-carboxylate

TABLE 1-8 326

2-(Dimethylamino)ethyl 5-[(1H- pyrrolo[2,3-b]pyridin-3-yl)methylene]- 2-(cyclohexylamino)-4-oxo-4,5- dihydrofuran-3-carboxylate 327

2-(Dimethylamino)ethyl 5-[(1H- pyrrolo[2,3-b]pyridin-3-yl)methylene]- 2-(4-methylpiperidino)-4-oxo-4,5- dihydrofuran-3-carboxylate 328

3-Hydroxypropyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2-azepinyl- 4-oxo-4,5-dihydrofuran-3-carboxylate 329

3-Hydroxypropyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2-(4- methylpiperidino)-4-oxo-4,5- dihydrofuran-3-carboxylate 330

2-Methoxyethyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2-({2- methyl-4-[2-(pyrrolidinyl)ethoxy] phenyl}amino)-4-oxo-4,5-dihydrofuran- 3-carboxylate acetate 331

3-Hydroxypropyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2-(3,4- dihydroisoquinolin-2(1H)-yl)-4-oxo- 4,5-dihydrofuran-3-carboxylate 332

3-Hydroxypropyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2- (morpholinoamino)-4-oxo-4,5- dihydrofuran-3-carboxylate 333

3-Hydroxypropyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2-(2,2- dimethylhydrazinyl)-4-oxo-4,5- dihydrofuran-3-carboxylate 334

2-Methoxyethyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2- (morpholinoamino)-4-oxo-4,5- dihydrofuran-3-carboxylate 335

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(4,5-dihydrofuro [2, 3-c] pyridin-6(7H)-yl)-4-oxo-4,5- dihydrofuran-3-carboxylate 336

3-Hydroxypropyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2-({4- [(2-hydroxyethyl)amino]-2- methylphenyl}amino)-4-oxo-4,5- dihydrofuran-3-carboxylate 337

3-Hydroxypropyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2-({4- [(dimethylamino)methyl]-2- methylphenyl}amino)-4-oxo-4,5- dihydrofuran-3-carboxylate hydrochloride 338

2-(Dimethylamino)ethyl 5-[(1H-pyrrolo [2,3-b]pyridin-3-yl)methylene]- 2-(2,2-dimethylhydrazinyl)-4-oxo- 4,5-dihydrofuran-3-carboxylate acetate

TABLE 1-9 339

2-(Dimethylamino)ethyl 5-[(1H- pyrrolo[2,3-b]pyridin-3-yl)methylene]- 2-(morpholinoamino)-4-oxo-4,5- dihydrofuran-3-carboxylate acetate 340

2-(Dimethylamino)ethyl 5-[(1H-pyrrolo [2,3-b]pyridin-3-yl)methylene]- 2-({4-[(2-hydroxyethyl) amino]-2- methylphenyl}amino)-4-oxo-4,5- dihydrofuran-3-carboxylate acetate 341

Ethyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-{[4-(cyanomethyl)- 2-methylphenyl]amino}-4-oxo-4,5- dihydrofuran-3-carboxylate 342

2-(Dimethylamino)ethyl 5-[(1H-pyrrolo [2,3-b]pyridin-3-yl)methylene]- 2-({4-[(dimethylamino)methyl]-2- methylphenyl}amino)-4-oxo-4,5- dihydrofuran-3-carboxylate diformate 343

Isopropyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-{[4-(2-hydroxyethoxy)- 2-methylphenyl]amino}-4- oxo-4,5-dihydrofuran-3-carboxylate 344

Propyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(morpholinoamino)- 4-oxo-4,5-dihydrofuran-3-carboxylate 345

Isobutyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(morpholinoamino)- 4-oxo-4,5-dihydrofuran-3-carboxylate 346

2-Hydroxyethyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2- (morpholinoamino)-4-oxo-4,5- dihydrofuran-3-carboxylate 347

Cyclopropylmethyl 5-[(1H-pyrrolo [2,3-b]pyridin-3-yl)methylene]-2- (morpholinoamino)-4-oxo-4,5- dihydrofuran-3-carboxylate 348

Cyclopentyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2- (morpholinoamino)-4-oxo-4,5- dihydrofuran-3-carboxylate 349

(S)-sec-Butyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2- (morpholinoamino)-4-oxo-4,5- dihydrofuran-3-carboxylate 350

(R)-sec-Butyl 5-[(1H-pyrrolo[2,3-b] pyridin-3-yl)methylene]-2- (morpholinoamino)-4-oxo-4,5- dihydrofuran-3-carboxylate 351

Isopropyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-4-oxo-2-{[4- (2,2,2-trifluoroethyl)piperazinyl] amino}-4,5-dihydrofuran-3-carboxylate

TABLE 1-10 352

Isopropyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-4-oxo-2-[(tetrahydro- 2H-pyran-4-yl)amino]-4,5-dihydrofuran- 3-carboxylate 353

Isopropyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-4-oxo-2-[(1,1- dioxidothiomorpholino)amino]-4,5- dihydrofuran-3-carboxylate 354

Isopropyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(7-methoxy- 3,4-dihydroisoquinolin-2(1H)-yl)-4- oxo-4,5-dihydrofuran-3-carboxylate 355

Isopropyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(7-methyl-? 3,4-dihydroisoquinolin-2 (1H)-yl)-4- oxo-4,5-dihydrofuran-3-carboxylate acetate 356

Isopropyl 5-[(1H-pyrrolo[2,3-b]pyrlidin- 3-yl)methylene]-2-(7-chloro- 3,4-dihydroisoquinolin-2(1H)-yl)-4- oxo-4,5-dihydrofuran-3-carboxylate acetate 357

Isopropyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(7-fluoro- 3,4-dihydroisoquinolin-2(1H)-yl)-4- oxo-4,5-dihydrofuran-3-carboxylate 358

Isopropyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(5-methoxy- 3,4-dihydroisoquinolin-2(1H)-yl)-4- oxo-4,5-dihydrofuran-3-carboxylate 359

Isopropyl 5-[(1H-pyrrolo[2,3-b]pyridin- 3-yl)methylene]-2-(6-methoxy- 3,4-dihydroisoquinolin-2(1H)-yl)-4- oxo-4,5-dihydrofuran-3-carboxylate

TABLE 2-1 LCMS m/z Example ¹H-NMR δ (ppm) [M + H]⁺ 247 (DMSO-d6): 12.17-12.43 (m, 1H), 8.18-8.41 (m, 3H), 7.94 (br. s, 1H), 7.12- 383.2 7.23 (m, 1H), 6.85 (br. s, 1H), 4.10-4.23 (m, 2H), 3.65-3.93 (m, 2H), 2.93- 3.13 (m, 2H), 2.77-2.93 (m, 2H), 1.80-1.98 (m, 2H), 1.10-1.35 (m, 5H). 248 (DMSO-d6): 12.37 (s, 1H), 8.40 (d, J = 7.6 Hz, 1H), 8.33 (d, J = 3.7 Hz, 1H), 422.0 8.09 (s, 1H), 7.47 (d, J = 4.9 Hz, 1H), 7.21 (dd, J = 7.7, 4.7 Hz, 1H), 6.87- 6.98 (m, 2H), 5.07 (s, 2H), 4.21 (q, J = 6.9 Hz, 2H), 3.98-4.08 (m, 2H), 2.92- 3.03 (m, 2H), 1.26 (t, J = 7.0 Hz, 3H). 249 (DMSO-d6): 12.37 (s, 1H), 8.28-8.39 (m, 2H), 8.02 (br. s, 1H), 7.10-7.29 430.0 (m, 5H), 6.81 (s, 1H), 5.13 (s, 2H), 4.18-4.30 (m, 2H), 4.00-4.18 (m, 2H), 2.99-3.12 (m, 2H), 1.92-2.07 (m, 2H), 1.28 (t, J = 6.6 Hz, 3H). 250 (DMSO-d6): 12.42 (s, 1H), 8.27-8.38 (m, 2H), 7.97 (s, 1H), 7.30-7.43 (m, 1H), 432.3 7.15-7.27 (m, 2H) 7.03-7.13 (m, 1H), 6.96 (d, J = 7.7 Hz, 1H), 6.85 (s, 1H), 5.18 (s, 2H), 4.40-4.50 (m, 2H), 4.14-4.26 (m, 2H), 4.00-4.14 (m, 2H), 1.26 (t, J = 6.6 Hz, 3H). 251 (DMSO-d6): 12.14 (s, 1H), 10.70-12.05 (m, 1H), 8.23 (d, J = 4.0 Hz, 1H), 8.00 423.2 (d, J = 7.8 Hz, 1H), 7.69 (s, 1H), 7.20-7.50 (m, 5H), 6.91 (dd, J = 7.7, 4.7 Hz, 1H), 4.51 (s, 2H). 252 (DMSO-d6): 12.33 (br. s, 1H), 8.57 (d, J = 6.02 Hz, 1H), 8.42 (dd, J = 1.38, 358.0 7.91 Hz, 1H), 8.31 (dd, J = 1.25, 4.52 Hz, 1H), 8.01 (s, 1H), 7.20 (dd, J = 4.77, 8.03 Hz, 1H), 6.90 (s, 1H), 5.20 (t, J = 5.27 Hz, 1H), 4.16-4.34 (m, 3H), 3.52-3.68 (m, 2H), 1.32 (d, J = 6.53 Hz, 3H), 1.26 (t, J = 7.15 Hz, 3H). 253 (DMSO-d6): 12.38 (br. s, 1H), 8.93 (br. s, 1H), 8.43 (dd, J = 1.38, 7.91 Hz, 414.0 1H), 8.31 (dd, J = 1.38, 4.64 Hz, 1H), 8.06 (s, 1H), 7.19 (dd, J = 4.64, 7.91 Hz, 1H), 6.90 (s, 1H), 4.37-4.46 (m, 1H), 4.22 (q, J = 7.03 Hz, 2H), 4.06 (dd, J = 6.53, 8.78 Hz, 1H) 3.68-3.89 (m, 3H), 1.32 (s, 3H), 1.23-1.29 (m, 6H). 254 (DMSO-d6): 12.35 (br. s, 1H), 8.84 (br. s, 1H), 8.46 (dd, J = 1.38, 7.91 Hz, 358.0 1H), 8.31 (dd, J = 1.38, 4.64 Hz, 1H), 8.04 (s, 1H), 7.19 (dd, J = 4.77, 8.03 Hz, 1H), 6.89 (s, 1H), 5.17 (d, J = 4.52 Hz, 1H), 4.21 (q, J = 7.03 Hz, 2H), 3.8 9-4.00 (m, 1H) 3.57-3.67 (m, 1H), 3.45-3.56 (m, 1H), 1.26 (t, J = 7.15 Hz, 3H), 1.16 (d, J = 6.27 Hz, 3H). 255 (DMSO-d6): 12.41 (br. s, 1H), 9.29 (br. s, 1H), 8.49 (d, J = 7.78 Hz, 1H), 8.32 338.0 (dd, J = 1.13, 4.64 Hz, 1H), 8.12 (s, 1H), 7.19 (dd, J = 4.64, 7.91 Hz, 1H), 6.96 (s, 1H), 4.43 (d, J = 1.51 Hz, 2H), 4.22 (q, J = 7.03 Hz, 2H), 3.42 (t, J = 2.26 Hz, 1H), 1.26 (t, J = 7.15 Hz, 3H). 256 (DMSO-d6): 12.35 (s, 1H), 11.34 (s, 1H), 10.67 (s, 1H), 8.33 (d, J = 7.3 Hz, 419.2 1H), 8.10 (d, J = 4.4 Hz, 1H), 7.94-8.05 (m, 3H), 7.68-7.76 (m, 1H), 7.58- 7.68 (m, 2H), 6.93 (s, 1H), 6.29-6.36 (m, 1H), 4.26 (q, J = 7.0 Hz, 2H), 1.29 (t, J = 7.0 Hz, 3H). 257 (DMSO-d6): 12.20-12.50 (m, 1H), 8.26-8.40 (m, 2H), 7.89 (s, 1H), 7.15-7.59 448.2 (m, 5H), 6.84 (s, 1H), 5.03-5.30 (m, 2H), 4.12-4.31 (m, 4H), 3.10-3.50 (m, 2H), 1.22-1 .32 (m, 3H). 258 (DMSO-d6): 12.10-12.60 (m, 1H), 8.25-8.40 (m, 2H), 7.93 (s, 1H), 7.12-7.24 431.2 (m, 2H), 6.96-7.05 (m, 1H), 6.82 (s, 1H), 6.66-6.78 (m, 2H), 5.85 (s, 1H), 5.05 (s, 2H), 4.15-4.30 (m, 2H), 3.75-4.00 (m, 2H), 3.40-3.55 (m, 2H), 1.26 (t, J = 7.0 Hz, 3H). 259 (DMSO-d6): 12.33 (br. s, 1H), 8.40 (d, J = 7.8 Hz, 1H), 8.32 (d, J = 4.3 Hz, 446.2 1H), 8.07 (s, 1H), 7.17-7.29 (m, 2H), 6.80-6.95 (m 3H), 4.96 (s, 2H), 4.21 (q, J = 7.0 Hz, 2H), 3.90-4.07 (m, 2H), 3.81 (s, 3H), 2.88-3.00 (m, 2H), 1.26 (t, J = 7.1 Hz, 3H). 260 (DMSO-d6): 12.32 (s, 1H), 8.37 (d, J = 7.9 Hz, 1H), 8.28-8.34 (m, 1H), 8.01 398.2 (s, 1H), 7.19 (dd, J = 8.0, 4.5 Hz, 1H), 6.88 (s, 1H), 4.08-4.24 (m, 4H), 3.73- 3.87 (m, 2H), 2.95-3.06 (m, 2H), 1.24 (t, J = 7.0 Hz, 3H), 1.18 (d, J = 6.0 Hz, 6H). 261 (DMSO-d6): 12.35 (s, 1H), 8.66 (br. s, 1H), 8.41 (d, J = 7.9 Hz, 1H), 8.32 (d, 384.2 J = 3.5 Hz, 1H), 8.01 (s, 1H), 7.21 (dd, J = 7.9, 4.7 Hz, 1H), 6.90 (s, 1H), 4.18-4.30 (m, 3H), 3.89-4.00 (m, 2H), 3.40-3.53 (m, 2H), 1.82-1.96 (m, 4H), 1.25 (q, J = 7.1 Hz, 3H). 262 (DMSO-d6): 12.31 (s, 1H), 9.87 (s, 1H), 8.93 (d, J = 7.8 Hz, 1H), 8.30 (d, J = 369.2 3.6 Hz, 1H), 8.06 (d, J = 2.2 Hz, 1H), 7.14 (dd, J = 8.0, 4.6 Hz, 1H), 6.85 (s, 1H), 4.20 (q, J = 7.0 Hz, 2H), 3.04-3.17 (m, 4H), 1.85-1.98 (m, 4H), 1.26 (t, J = 7.1 Hz, 3H). 263 (DMSO-d6): 12.39 (s, 1H), 10.05 (s, 1H), 8.85 (d, J = 7.8 Hz, 1H), 8.33 (d, J = 397.2 3.8 Hz, 1H), 8.12 (s, 1H), 7.17 (dd, J = 7.8, 4.6 Hz, 1H), 6.87 (s, 1H), 4.20 (q, J = 7.0 Hz, 2H), 3.08-3.20 (m, 4H), 1.63-1.84 (m, 8H), 1.26 (t, J = 7.0 Hz, 3H). 264 (DMSO-d6): 12.26 (s, 1H), 8.16-8.24 (m, 2H), 7.85 (d, J = 7.9 Hz, 1H), 7.62 530.4 (s, 1H), 7.38 (d, J = 7.5 Hz, 1H), 7.31 (s, 1H), 7.25 (d, J = 7.6 Hz, 1H), 6.82 (dd, J = 7.8, 4.7 Hz, 1H), 6.75 (s, 1H), 4.20-4.30 (m, 2H), 3.57 (s, 2H), 3.10-3.60 (m, 8H), 2.68-2.77 (m, 1H), 2.24 (s, 3H), 1.29 (t, J = 7.1 Hz, 3H), 0.99 (d, J = 6.5 Hz, 6H). 265 (DMSO-d6): 8.21 (s, 1H), 8.17 (d, J = 3.4 Hz, 1H), 7.83 (d, J = 7.7 Hz, 1H), 487.2 7.62 (s, 1H), 7.40 (s, 1H), 7.33 (s, 2H), 6.81 (dd, J = 8.0, 4.7 Hz, 1H), 6.63 (s, 1H), 4.15-4.30 (m, 3H), 3.95 (s, 2H), 2.43-2.56 (m, 2H), 2.22 (s, 3H), 1.81-1.94 (m, 2H), 1.63-1.75 (m, 2H), 1.43-1.60 (m, 4H), 1.22-1.33 (m, 3H). 266 (DMSO-d6): 8.16 (d, J = 3.8 Hz, 1H), 7.83 (d, J = 7.6 Hz, 1H), 7.60 (s, 1H), 461.2 7.18-7.39 (m, 3H), 6.87 (s, 1H), 6.75-6.84 (m, 1H), 6.54 (s, 1H), 4.10-4.25 (m, 2H), 3.93 (s, 2H), 2.95-3.10 (m, 1H), 2.15-2.20 (m, 3H), 1.20-1.30 (m, 3H), 1.10-1.20 (m, 6H).

TABLE 2-2 267 (DMSO-d6): 12.26 (s, 1H), 10.32 (br. s, 1H), 8.20 (d, J = 3.5 Hz, 1H), 7.71- 503.2 7.82 (m, 2H), 7.46 (d, J = 8.2 Hz, 1H), 7.35 (s, 1H), 7.29 (d, J = 8.5 Hz, 1H), 6.83 (s, 1H), 6.73-6.81 (m, 1H), 4.53-4.61 (m, 1H), 4.20-4.32 (m, 2H), 3.42-3.62 (m, 3H), 2.68-2.80 (m, 2H), 2.26 (s, 3H), 2.03-2.20 (m, 2H), 1.67-1.80 (m, 2H), 1.36-1.51 (m, 2H), 1.29 (t, J = 7.0 Hz, 3H). 268 (DMSO-d6): 12.49 (br. s, 1H), 9.38 (br. s, 1H), 8.44 (d, J = 7.53 Hz, 1H), 8.33 339.0 (dd, J = 1.25, 4.52 Hz, 1H), 8.17 (d, J = 2.51 Hz, 1H), 7.21 (dd, J = 4.77, 8.03 Hz, 1H), 7.02 (s, 1H), 4.79 (s, 2H), 4.23 (q, J = 7.19 Hz, 2H), 1.26 (t, J = 7.03 Hz, 3H). 269 (DMSO-d6): 12.35 (br. s, 1H), 8.60 (d, J = 8.78 Hz, 1H), 8.45 (dd, J = 1.26, 373.9 8.03 Hz, 1H), 8.31 (dd, J = 1.25, 4.52 Hz, 1H), 8.04 (s, 1H), 7.19 (dd, J = 4.77, 8.03 Hz, 1H), 6.90 (s, 1H), 5.18 (t, J = 5.14 Hz, 2H), 4.11-4.27 (m, 3H), 3.67 (t, J = 5.40 Hz, 4H), 1.26 (t, J = 7.15 Hz, 3H). 270 (DMSO-d6): 12.40 (br. s, 1H), 8.84 (t, J = 5.90 Hz, 1H), 8.49 (d, J = 8.03 Hz, 374.0 1H), 8.32 (dd, J = 1.38, 4.64 Hz, 1H), 8.06 (d, J = 2.51 Hz, 1H), 7.21 (dd, J = 4.77, 8.03 Hz, 1H), 6.90 (s, 1H), 4.21 (q, J = 7.03 Hz, 2H), 3.73-3.83 (m, 2H), 3.33-3.66 (m, 5H), 1.26 (t, J = 7.03 Hz, 3H). 271 (DMSO-d6): 12.10-12.20 (m, 1H), 8.20-8.42 (m, 2H), 7.98 (s, 1H), 7.19 (dd, 468.3 J = 7.9, 4.6 Hz, 1H), 6.85 (s, 1H), 4.10-4.40 (m, 4H), 3.85-4.05 (m, 2H), 3.25-3.40 (m, 4H), 2.35-3.45 (m, 3H), 2.65-2.80 (m, 1H), 2.15-2.25 (m, 6H), 1.80-1.95 (m, 2H), 1.53-1.72 (m, 2H), 1.20-1.28 (m, 3H). 272 (DMSO-d6): 12.30 (s, 1H), 8.37 (d, J = 7.9 Hz, 1H), 8.31 (d, J = 4.2 Hz, 1H), 486.2 7.98 (s, 1H), 7.19 (dd, J = 7.8, 4.6 Hz, 1H), 6.84 (s, 1H), 4.58-4.73 (m, 3H), 4.16 (q, J = 7.0 Hz, 2H), 3.93-4.01 (m, 2H), 3.68-3.77 (m, 1H), 3.40- 3.56 (m, 3H), 3.31-3.39 (m, 2H), 3.16 (s, 3H), 1.74-1.90 (m, 3H), 1.58- 1.72 (m, 3H), 1.24 (t, J = 7.1 Hz, 3H). 273 (DMSO-d6): 12.35 (s, 1H), 8.41 (d, J = 6.8 Hz, 1H), 8.32 (d, J = 3.4 Hz, 1H), 446.0 8.06 (s, 1H), 7.22 (dd, J = 7.9, 4.6 Hz, 2H), 6.83-6.93 (m, 3H), 4.90 (s, 2H), 4.20 (q, J = 7.0 Hz, 2H), 3.84-4.00 (m, 2H), 3.76 (s, 3H), 3.01-3.09 (m, 2H), 1.26 (t, J = 7.1 Hz, 3H). 274 (DMSO-d6): 12.24 (s, 1H), 8.26-8.48 (m, 2H), 7.95 (s, 1H), 7.10-7.17 (m, 1H), 433.2 6.84-6.94 (m, 3H), 6.59 (d, J = 8.0 Hz, 1H), 4.90 (s, 2H), 4.77 (s, 2H), 4.15 (q, J = 7.1 Hz, 2H), 3.16 (s, 3H), 2.01 (s, 3H), 1.25-1.19 (m, 3H). 275 (DMSO-d6): 12.31 (s, 1H), 8.37 (d, J = 8.0 Hz, 1H), 8.31 (d, J = 3.6 Hz, 1H), 382.2 7.96 (s, 1H), 7.19 (dd, J = 7.9, 4.6 Hz, 1H), 6.84 (s, 1H), 4.00-4.25 (m, 4H), 3.22-3.32 (m, 1H), 3.05-2.95 (m, 1H), 1.75-1.91 (m, 3H), 1.60-1.74 (m, 1H), 1.17-1.33 (m, 4H), 0.95 (d, J = 6.2 Hz, 3H). 276 (DMSO-d6): 12.32 (s, 1H), 8.37 (d, J = 7.9 Hz, 1H), 8.31 (d, J = 4.6 Hz, 1H), 398.2 8.01 (s, 1H), 7.19 (dd, J = 7.9, 4.7 Hz, 1H), 6.88 (s, 1H), 4.12-4.22 (m, 4H), 3.76-3.86 (m, 2H), 2.96-3.06 (m, 2H), 1.24 (t, J = 7.1 Hz, 3H), 1.18 (d, J = 6.2 Hz, 6H). 277 (DMSO-d6): 12.37 (s, 1H), 9.96 (s, 1H), 8.99 (d, J = 7.8 Hz, 1H), 8.34 (d, 428.4 J = 3.3 Hz, 1H), 8.11 (d, J = 2.5 Hz, 1H), 7.23 (dd, J = 8.0, 4.7 Hz, 1H), 6.85 (s, 1H), 4.49 (t, J = 5.2 Hz, 1H), 4.21 (q, J = 7.1 Hz, 2H), 3.56 (q, J = 6.0 Hz, 2H), 2.98-3.12 (m, 4H), 2.57-2.77 (m, 4H), 2.45-2.55 (m, 2H), 1.26 (t, J = 7.1 Hz, 3H). 278 (DMSO-d6): 12.21 (br. s, 1H), 8.28-8.42 (m, 2H), 7.96 (s, 1H), 7.18 (dd, J = 396.2 7.9, 4.7 Hz, 1H), 6.84 (s, 1H), 4.06-4.23 (m, 4H), 2.72-2.88 (m, 2H), 1.75- 1.92 (m, 3H), 1.24 (t, J = 7.0 Hz, 3H), 0.86-1.00 (m, 7H). 279 (DMSO-d6): 12.27 (s, 1H), 9.22 (br. s, 1H), 6.4-8.6 (m, 7H), 4.15-4.25 (m, 367.2 2H), 1.18-1.30 (m, 3H). 280 (DMSO-d6): 12.10-12.65 (m, 1H), 8.25-8.53 (m, 2H), 8.01 (s, 1H), 7.14-7.29 446.0 (m, 2H), 6.80-6.95 (m, 3H), 4.68-4.93 (m, 2H), 4.15-4.27 (m, 2H), 3.93- 4.05 (m, 2H), 3.80-3.92 (m, 3H), 2.98-3.10 (m, 2H), 1.23-1.32 (m, 3H). 281 (DMSO-d6): 12.34 (s, 1H), 9.51 (br. s, 1H), 9.17 (d, J = 7.3 Hz, 1H), 8.33 (d, 411.2 J = 3.3 Hz, 1H), 8.12 (s, 1H), 7.18 (dd, J = 8.0, 4.6 Hz, 1H), 6.82 (s, 1H), 4.22 (q, J = 7.0 Hz, 2H), 2.95-3.10 (m, 2H), 1.70-1.85 (m, 3H), 1.37-1.53 (m, 3H), 1.27 (t, J = 7.1 Hz, 3H), 0.98 (d, J = 6.0 Hz, 6H). 282 (DMSO-d6): 12.25 (br. s, 1H), 8.34-8.39 (m, 1H), 8.32 (d, J = 4.6 Hz, 1H), 7.97 396.2 (s, 1H), 7.19 (dd, J = 7.9, 4.6 Hz, 1H), 6.84 (s, 1H), 4.10-4.27 (m, 2H), 3.73 (dd, J = 13.1, 3.5 Hz, 2H), 3.43-3.59 (m, 2H), 2.06-2.20 (m, 2H), 1.54 (t, J = 5.7 Hz, 2H), 1.25 (t, J = 6.7 Hz, 3H), 0.95 (d, J = 7.3 Hz, 6H). 283 (DMSO-d6): 12.30 (s, 1H), 8.37 (d, J = 7.9 Hz, 1H), 8.32 (d, J = 4.7 Hz, 1H), 396.2 7.97 (s, 1H), 7.19 (dd, J = 7.8, 4.7 Hz, 1H), 6.84 (s, 1H), 4.18 (q, J = 7.1 Hz, 2H), 3.62-3.71 (m, 2H), 3.54 (s, 2H), 1.71-1.82 (m, 2H), 1.45-1.56 (m, 2H), 1.25 (t, J = 7.1 Hz, 3H), 0.97 (s, 6H). 284 (DMSO-d6): 12.30 (s, 1H), 8.38 (d, J = 8.2 Hz, 1H), 8.31 (d, J = 4.2 Hz, 1H), 398.0 8.00 (s, 1H), 7.19 (dd, J = 8.0, 4.6 Hz, 1H), 6.83 (s, 1H), 4.97-5.10 (m, 1H), 4.42-4.56 (m, 1H), 4.10-4.23 (m, 2H), 3.80-3.95 (m, 2H), 3.57-3.72 (m, 2H), 1.70-1.90 (m, 3H), 1.55-1.70 (m, 3H), 1.24 (t, J = 7.0 Hz, 3H). 285 (DMSO-d6): 12.28 (br. s, 1H), 10.34 (br. s, 1H), 8.20 (dd, J = 1.38, 4.64 Hz, 503.3 1H), 7.73-7.79 (m, 2H), 7.42 (d, J = 7.78 Hz, 1H), 7.31 (s, 1H), 7.25 (d, J = 8.03 Hz, 1H), 6.84 (s, 1H), 6.76 (dd, J = 4.64, 7.91 Hz, 1H), 4.27 (q, J = 7.03 Hz, 2H), 3.61 (t, J = 4.52 Hz, 4H), 3.28-3.34 (m, 4H), 2.82-2.90 (m, 2H), 2.58-2.64 (m, 2H), 2.24 (s, 3H), 1.30 (t, J = 7.03 Hz, 3H). 286 (DMSO-d6): 12.25 (s, 1H), 10.37 (s, 1H), 8.20 (d, J = 3.7 Hz, 1H), 7.78 (d, J = 434.0 7.8 Hz, 1H), 7.72 (s, 1H), 7.42 (d, J = 8.0 Hz, 1H), 7.30 (s, 1H), 7.23 (d, J = 7.7 Hz, 1H), 6.85 (s, 1H), 6.80 (dd, J = 7.8, 4.7 Hz, 1H), 4.77 (t, J = 5.1 Hz, 1H), 4.27 (q, J = 6.9 Hz, 2H), 3.72 (q, J = 6.7 Hz, 2H), 2.85 (t, J = 6.9 Hz, 2H), 2.24 (s, 3H), 1.30 (t, J = 7.0 Hz, 3H).

TABLE 2-3 287 (DMSO-d6): 12.11 (br. s, 1H), 8.15 (d, J = 3.8 Hz, 1H), 7.68-7.78 (m, 2H), 7.43 497.2 (s, 1H), 7.26-7.38 (m, 2H), 6.80 (dd, J = 7.7, 4.8 Hz, 1H), 6.60 (s, 1H), 4.15- 4.25 (m, 2H), 3.66 (s, 2H), 2.60-2.70 (m, 4H), 2.19 (s, 3H), 1.91 (s, 3H), 1.70- 1.82 (m, 4H), 1.27 (t, J = 7.0 Hz, 3H). 288 (DMSO-d6): 11.80-12.15 (m, 1H), 8.22-8.37 (m, 2H), 8.12-8.18 (m, 1H), 7.74- 513.0 7.83 (m, 1H), 7.54-7.72 (m, 1H), 7.33 (s, 1H), 7.26 (d, J = 8.9 Hz, 1H), 6.9- 7.05 (m, 1H), 6.77-6.84 (m, 1H), 6.27-6.39 (m, 1H), 4.04-4.23 (m, 2H), 3.60- 3.70 (m, 4H), 3.56 (s, 2H), 2.53-2.62 (m, 4H), 2.10 (s, 3H), 1.20-1.30 (m, 3H). 289 (DMSO-d6): 8.05-8.15 (m, 1H), 7.82 (d, J = 7.4 Hz, 1H), 7.52 (s, 1H), 7.27 (s, 1H), 527.0 7.20 (d, J = 7.8 Hz, 1H), 6.91 (d, J = 7.9 Hz, 1H), 6.75-6.84 (m, 1H), 6.18 (s, 1H), 4.02-4.13 (m, 2H), 3.30-3.70 (m, 3H), 2.75-2.85 (m, 2H), 2.36-2.26 (m, 2H), 2.07 (s, 3H), 1.72-1.82 (m, 2H), 1.40-1.50 (m, 2H), 1.15-1.30 (m, 3H). 290 (DMSO-d6): 12.27 (br. s, 1H), 8.20 (dd, J = 1.25, 4.77 Hz, 1H), 7.72-7.80 (m, 2H), 516.3 7.40 (d, J = 8.03 Hz, 1H), 7.30 (s, 1H), 7.23 (d, J = 7.78 Hz, 1H), 6.82 (s, 1H), 6.76 (dd, J = 4.64, 7.91 Hz, 1H), 4.26 (q, J = 7.03 Hz, 2H), 3.25-3.50 (m, 4H), 2.79-2.89 (m, 2H), 2.56-2.66 (m, 2H), 2.31-3.47 (m, 4H), 2.23 (s, 3H), 2.20 (s, 3H), 1.29 (t, J = 7.15 Hz, 3H). 291 (DMSO-d6): 12.31 (s, 1H), 8.39 (d, J = 6.9 Hz, 1H), 8.31 (d, J = 4.6 Hz, 1H), 7.95 342.2 (s, 1H), 7.20 (dd, J = 7.9, 4.7 Hz, 1H), 6.84 (s, 1H), 4.17 (q, J = 7.1 Hz, 2H), 3.73 (q, J = 7.0 Hz, 2H), 3.26 (s, 3H), 1.20-1.33 (m, 6H). 292 (DMSO-d6): 8.38 (d, J = 7.9 Hz, 1H), 8.31 (d, J = 3.3 Hz, 1H), 7.95 (s, 1H), 7.19 356.0 (dd, J = 7.9, 4.7 Hz, 1H), 6.84 (s, 1H), 4.50-4.62 (m, 1H), 4.17 (d, J = 7.1 Hz, 2H), 3.09 (s, 3H), 1.36-1.20 (m, 9H). 293 (DMSO-d6): 12.31 (br. s, 1H), 10.01 (br. s, 1H), 8.92 (d, J = 7.8 Hz, 1H), 8.31 399.2 (d, J = 3.7 Hz, 1H), 8.08 (s, 1H), 7.18 (dd, J = 7.9, 4.7 Hz, 1H), 6.83 (s, 1H), 4.97 (br. s, 1H), 4.20 (q, J = 7.0 Hz, 2H), 3.72-3.83 (m, 1H), 3.12-3.20 (m, 1H), 2.97-3.06 (m, 1H), 2.71-2.91 (m, 2H), 1.80-1.93 (m, 2H), 1.56-1.68 (m, 1H), 1.18-1.31 (m, 4H). 294 (DMSO-d6): 12.29 (s, 1H), 8.29-8.38 (m, 2H), 7.96 (s, 1H), 7.19 (dd, J = 7.9, 396.4 4.7 Hz, 1H), 6.82 (s, 1H), 4.95-5.10 (m, 1H), 4.15-4.25 (m, 2H), 3,24-3.34 (m, 2H), 1.68-1.88 (m, 3H), 1.25-1.40 (m, 2H), 1.25 (d, J = 6.2 Hz, 6H), 0.97 (d, J = 6.3 Hz, 3H). 295 (DMSO-d6): 12.35 (s, 1H), 8.40 (d, J = 6.6 Hz, 1H), 8.33 (d, J = 4.6 Hz, 1H), 8.07 430.2 (s, 1H), 7.24-7.32 (m, 4H), 7.22 (dd, J = 7.9, 4.7 Hz, 1H), 6.87 (s, 1H), 5.00- 5.12 (m, 1H), 4.97 (s, 2H), 3.90-4.02 (m, 2H), 3.03-3.13 (m, 2H), 1.27 (t, J = 8.9 Hz, 6H). 296 (DMSO-d6): 12.31 (s, 1H), 9.86 (s, 1H), 8.84 (d, J = 7.8 Hz, 1H), 8.30 (d, J = 3.3 357.2 Hz, 1H), 8.05 (d, J = 2.5 Hz, 1H), 7.20 (dd, J = 8.0, 4.6 Hz, 1H), 6.83 (s, 1H), 4.99-5.10 (m, 1H), 2.77 (s, 6H), 1.27 (d, J = 6.3 Hz, 6H). 297 (DMSO-d6): 12.26 (s, 1H), 10.07 (s, 1H), 8.17 (s, 1H), 7.85 (s, 1H), 7.69 (d, J = 462.8 7.4 Hz, 1H), 7.17 (d, J = 8.3 Hz, 1H), 6.72-6.88 (m, 2H), 6.52-6.67 (m, 2H), 5.80-5.92 (m, 1H), 5.07-5.18 (m, 1H), 4.71-4.81 (m, 1H), 3.58-3.69 (m, 2H), 3.15-3.25 (m, 2H), 2.13 (s, 3H), 1.30 (d, J = 5.9 Hz, 6H). 298 (MeOD): 8.15-8.24 (m, 1H), 7.86 (d, J = 7.5 Hz, 1H), 7.65 (br. s, 1H), 7.40-7.55 526.4 (m, 3H), 6.90-7.03 (m, 2H), 6.61 (s, 1H), 4.34-4.43 (m, 2H), 3.66 (s, 2H), 2.70-2.98 (m, 8H), 2.49 (s, 3H), 2.32 (s, 3H), 1.35-1.42 (m, 3H). 299 (DMSO-d6): 12.31 (s, 1H), 10.42 (br. s, 1H), 8.23 (dd, J = 4.6, 1.4 Hz, 1H), 7.79 487.0 (d, J = 7.3 Hz, H), 7.72 (d, J = 2.7 Hz, 1H), 7.51 (d, J = 8.0 Hz, 1H), 7.37 (s, 1H), 7.30 (d, J = 7.9 Hz, 1H), 6.87 (s, 1H), 6.78 (dd, J = 7.9, 4.8 Hz, 1H), 4.27 (q, J = 7.1 Hz, H), 3.35-3.70 (m, 4H), 3.03-3.15 (m, 4H), 2.27 (s, 3H), 1.85- 2.10 (s, 4H), 1.30 (t, J = 7.1 Hz, 3H). 300 (DMSO-d6): 12.30 (br. s, 1H), 8.39 (d, J = 7.6 Hz, 1H), 8.31 (d, J = 4.4 Hz, 1H), 396.0 7.93 (s, 1H), 7.19 (dd, J = 7.9, 4.7 Hz, 1H), 6.85 (s, 1H), 4.16 (q, J = 7.1 Hz, 3H), 3.11 (s, 3H), 1.79-1.92 (m, 4H), 1.60-1.75 (m, 3H), 1.28-1.44 (m, 2H), 1.11-1.28 (m, 4H). 301 (DMSO-d6): 12.30 (br. s, 1H), 9.62 (br. s, 1H), 8.91 (d, J = 8.0 Hz, 1H), 8.31 (d, 411.0 J = 3.8 Hz, 1H), 8.07 (s, 1H), 7.16 (dd, J = 8.0, 4.6 Hz, 1H), 6.84 (s, 1H), 4.21 (q, J = 7.0 Hz, 2H), 2.78-2.88 (m, 1H), 2.77 (s, 3H), 1.88-2.00 (m, 2H), 1.68- 1.80 (m, 2H), 1.48-1.59 (m, 1H), 1.16-1.31 (m, 7H), 1.00-1.12 (m, 1H). 302 (DMSO-d6): 12.31 (s, 1H), 10.07 (s, 1H), 8.94 (d, J = 7.5 Hz, 1H), 8.32 (d, J = 426.0 3.4 Hz, 1H), 8.12 (s, 1H), 7.21 (dd, J = 8.0, 4.7 Hz, 1H), 6.88 (s, 1H), 4.21 (q, J = 7.1 Hz, 2H), 3.57-3.76 (m, 4H), 2.97-3.12 (m, 4H), 2.08 (s, 3H), 1.26 (t, J = 7.1 Hz, 3H). 303 (DMSO-d6): 12.30 (br. s, 1H), 8.15-8.21 (m, 2H), 7.77 (s, 1H), 7.68 (d, J = 7.8 517.4 Hz, 1H), 7.42 (d, J = 8.7 Hz, 1H), 7.04 (s, 1H), 6.94 (d, J = 8.7 Hz, 1H), 6.73- 6.81 (m, 2H), 5.08-5.19 (m, 1H), 4.19 (t, J = 5.6 Hz, 2H), 2.94 (t, J = 5.6 Hz, 2H), 2.62-2.71 (m, 4H), 2.23 (s, 3H), 1.70-1.80 (m, 4H), 1.31 (d, J = 6.2 Hz, 6H). 304 (DMSO-d6): 12.25 (br. s, 1H), 9.80 (br. s, 1H), 8.85 (d, J = 7.4 Hz, 1H), 8.29 (d, 357.2 J = 3.6 Hz, 1H), 8.04 (s, 1H), 7.17 (dd, J = 8.0, 4.7 Hz, 1H), 6.78 (br. s, 1H), 4.19 (q, J = 6.9 Hz, 2H), 2.88-3.00 (m, 2H), 2.74 (s, 3H), 1.25 (t, J = 7.1 Hz, 3H), 1.05 (t, J = 7.1 Hz, 3H). 305 (DMSO-d6): 12.31 (br. s, 1H), 9.49 (br. s, 1H), 8.96 (d, J = 7.8 Hz, 1H), 8.31 (d, 371.2 J = 3.3 Hz, 1H), 8.08 (s, 1H), 7.18 (dd, J = 8.0, 4.6 Hz, 1H), 6.86 (s, 1H), 4.22 (q, J = 7.1 Hz, 2H), 3.00 (q, J = 7.0 Hz, 4H), 1.27 (t, J = 7.1 Hz, 3H), 1.04 (t, J = 7.1 Hz, 6H).

TABLE 2-4 306 (DMSO-d6): 12.09 (br. s, 1H), 8.98 (d, J = 7.6 Hz, 1H), 8.32 (d, J = 3.4 Hz, 1H), 399.2 8.05 (s, 1H), 7.21 (dd, J = 8.0, 4.7 Hz, 1H), 6.73 (s, 1H), 4.80 (s, 1H), 4.17 (q, J = 7.0 Hz, 2H), 3.54-3.69 (m, 1H), 3.04-3.15 (m, 2H), 2.82-2.95 (m, 2H), 1.83-1.94 (m, 2H), 1.63-1.77 (m, 2H), 1.24 (t, J = 7.0 Hz, 3H). 307 (DMSO-d6): 12.29 (s, 1H), 10.23 (s, 1H), 8.18 (d, J = 3.4 Hz, 1H), 7.78 (d, J = 533.4 2.5 Hz, 1H), 7.66 (d, J = 7.9 Hz, 1H), 7.42 (d, J = 8.6 Hz, 1H), 7.05 (d, J = 2.4 Hz, 1H), 6.94 (dd, J = 8.6, 2.7 Hz, 1H), 6.79 (s, 1H), 6.74 (dd, J = 7.9, 4.7 Hz, 1H), 5.09-5.18 (m, 1H), 4.19 (t, J = 5.6 Hz, 2H), 3.58-3.67 (m, 4H), 2.78 (t, J = 5.6 Hz, 2H), 2.49-2.57 (m, 4H), 2.23 (s, 3H), 1.31 (d, J = 6.3 Hz, 6H). 308 (DMSO-d6): 12.20 (br. s, 1H), 10.26 (br. s, 1H), 8.18 (d, J = 3.9 Hz, 1H), 7.66- 546.6 7.77 (m, 2H), 7.26-7.39 (m, 1H), 7.00 (s, 1H), 6.86-6.93 (m, 1H), 6.61-6.78 (s, 2H), 5.06-5.17 (m, 1H), 4.11-4.18 (m, 2H), 2.76 (t, J = 5.5 Hz, 2H), 2.30- 2.50 (m, 8H), 2.20 (s, 3H), 2.17 (s, 3H), 1.29 (d, J = 6.2 Hz, 6H). 309 (DMSO-d6): 12.30 (br. s, 1H), 8.42 (d, J = 8.0 Hz, 1H), 8.32 (d, J = 3.3 Hz, 1H), 412.2 7.98 (s, 1H), 7.20 (dd, J = 8.0, 4.7 Hz, 1H), 6.89 (s, 1H), 4.23-4.35 (m, 2H), 3.96-4.07 (m, 1H), 3.54-3.64 (m, 2H), 3.30 (s, 3H), 1.94-2.03 (m, 2H), 1.73- 1.84 (m, 2H), 1.51-1.69 (m, 3H), 1.35-1.49 (m, 2H), 1.15-1.28 (m, 1H). 310 (DMSO-d6): 12.32 (s, 1H), 9.84 (s, 1H), 8.83 (d, J = 8.2 Hz, 1H), 8.30 (d, J = 3.4 373.4 Hz, 1H), 8.05 (s, 1H), 7.20 (dd, J = 8.0, 4.5 Hz, 1H), 6.88 (s, 1H), 4.25-4.32 (m, 2H), 3.57-3.63 (m, 2H), 3.30 (s, 3H), 2.78 (s, 6H). 311 (DMSO-d6): 12.31 (s, 1H), 10.43 (s, 1H), 8.23 (d, J = 3.8 Hz, 1H), 7.73-7.81 (m, 444.4 2H), 7.58 (d, J = 8.1 Hz, 1H), 7.53 (s, 1H), 7.44 (d, J = 7.9 Hz, 1H), 6.88 (s, 1H), 6.83 (dd, J = 7.9, 4.7 Hz, 1H), 5.42 (t, J = 5.9 Hz, 1H), 4.38 (d, J = 5.9 Hz, 2H), 4.27 (q, J = 7.0 Hz, 2H), 2.27 (s, 3H), 1.30 (t, J = 7.0 Hz, 3H). 312 (DMSO-d6): 12.27 (s, 1H), 10.41 (s, 1H), 8.16 (d, J = 3.4 Hz, 1H), 7.79 (d, J = 1.9 471.1 Hz, 1H), 7.64 (d, J = 7.5 Hz, 1H), 7.48-755 (m, 2H), 7.42 (d, J = 7.9 Hz, 1H), 6.82 (s, 1H), 6.79 (dd, J = 7.9, 4.6 Hz, 1H), 4.24 (q, J = 7.0 Hz, 2H), 3.53 (s, 2H), 2.30 (s, 6H), 2.23 (s, 3H), 1.27 (t, J = 7.0 Hz, 3H). 313 (DMSO-d6): 12.28 (s, 1H), 10.17 (br. s, 1H), 8.21 (d, J = 3.3 Hz, 1H), 7.71-7.80 461.1 (m, 2H), 7.44 (d, J = 8.1 Hz, 1H), 7.32 (s, 1H), 7.25 (d, J = 8.0 Hz, 1H), 6.84 (s, 1H), 6.75-6.81 (m, 1H), 4.23-4.32 (m, 2H), 2.85-2.95 (m, 2H), 2.75-2.85 (m, 2H), 2.42 (s, 6H), 2.24 (s, 3H), 1.30 (t, J = 7.1 Hz, 3H). 314 (DMSO-d6): 12.28 (s, 1H), 10.27 (br. s, 1H), 8.21 (d, J = 4.6 Hz, 1H), 7.72-7.78 517.1 (m, 2H), 7.42 (d, J = 8.0 Hz, 1H), 7.30 (s, 1H), 7.24 (d, J = 8.3 Hz, 1H), 6.84 (s, 1H), 6.76 (dd, J = 7.9, 4.6 Hz, 1H), 4.58-4.72 (m, 1H), 4.22-4.32 (m, 2H), 3.44-3.60 (m, 1H), 2.80-3.00 (m, 4H), 2.40-2.60 (m, 4H), 2.24 (s, 3H), 1.72- 1.85 (m, 2H), 1.38-1.53 (m, 2H), 1.29 (t, J = 7.1 Hz, 3H). 315 (DMSO-d6): 12.26 (br. s, 1H), 9.72 (br. s, 1H), 8.91 (d, J = 7.6 Hz, 1H), 8.30 (d, 371.0 J = 3.7 Hz, 1H), 8.05 (s, 1H), 7.16 (dd, J = 7.8, 4.6 Hz, 1H), 6.77 (br. s, 1H), 4.13-4.24 (m, 2H), 3.08-3.20 (m, 1H), 2.72 (s, 3H), 1.25 (t, J = 7.1 Hz, 3H), 1.10 (d, J = 6.3 Hz, 6H). 316 (DMSO-d6): 12.33 (br. s, 1H), 10.25 (br. s, 1H), 8.87-8.99 (m, 1H), 8.30 (d, J = 398.0 3.4 Hz, 1H), 8.01-8.18 (m, 2H), 7.07 (dd, J = 8.0, 4.6 Hz, 1H), 6.85 (br. s, 1H), 4.15-4.26 (m, 2H), 3.60-3.72 (m, 2H), 3.20-3.30 (m, 4H), 1.26 (t, J = 7.0 Hz, 3H). 317 (DMSO-d6): 12.36 (s, 1H), 8.42 (d, J = 7.3 Hz, 1H), 8.33 (d, J = 3.4 Hz, 1H), 8.07 446.1 (s, 1H), 7.24-7.32 (m, 4H), 7.22 (dd, J = 7.9, 4.7 Hz, 1H), 6.91 (s, 1H), 4.98 (br. s, 2H), 4.25-4.33 (m, 2H), 3.89-4.03 (m, 2H), 3.56-3.64 (m, 2H), 3.30 (s, 3H), 3.08 (t, J = 5.7 Hz, 2H). 318 (DMSO-d6): 12.26 (s, 1H), 10.09 (s, 1H), 8.18 (d, J = 3.3 Hz, 1H), 7.83 (s, 1H), 479.1 7.70 (d, J = 7.7 Hz, 1H), 7.16 (d, J = 8.5 Hz, 1H), 6.75-6.86 (m, 2H), 6.61 (s, 1H), 6.57 (d, J = 8.5 Hz, 1H), 5.81-5.89 (m, 1H), 4.76 (t, J = 5.4 Hz, 1H), 4.28- 4.36 (m, 2H), 3.58-3.67 (m, 4H), 3.32 (s, 1H), 3.20 (q, J = 5.9 Hz, 2H), 2.13 (s, 3H). 319 (DMSO-d6): 12.24 (br. s, 1H), 8.36 (d, J = 8.0 Hz, 1H), 8.31 (d, J = 4.5 Hz, 1H), 451.2 8.00 (s, 1H), 7.20 (dd, J = 7.9, 4.6 Hz, 1H), 6.88 (s, 1H), 4.16 (q, J = 7.1 Hz, 2H), 3.76-3.86 (s, 4H), 3.26-3.32 (m, 2H), 2.82-2.90 (m, 4H), 1.25 (q, J = 7.0 Hz, 3H). 320 (DMSO-d6): 12.22 (br. s, 1H), 8.19 (s, 1H), 7.83 (d, J = 7.7 Hz, 1H), 7.72 (s, 461.4 1H), 7.21-7.47 (m, 3H), 6.70-6.85 (m, 2H), 5.06-5.18 (m, 1H), 3.49 (s, 2H), 2.25 (s, 3H), 2.22 (s, 6H), 1.91 (s, 3H), 1.30 (d, J = 5.8 Hz, 6H). 321 (DMSO-d6): 8.27-8.35 (m, 2H), 7.95 (s, 1H), 7.24-7.18 (m, 1H), 6.84 (s, 1H), 412.1 4.21-4.27 (m, 2H), 3.54-3.62 (m, 4H), 3.23-3.32 (m, 5H), 1.70-1.85 (m, 3H), 1.23-1.37 (m, 2H), 0.94 (d, J = 6.2 Hz, 3H). 322 (DMSO-d6): 12.27 (s, 1H), 10.24 (s, 1H), 8.16-8.23 (m, 1H), 7.76 (s, 1H), 7.68 548.8 (d, J = 7.9 Hz, 1H), 7.42 (d, J = 8.6 Hz, 1H), 7.05 (s, 1H), 6.95 (d, J = 8.1 Hz, 1H), 6.83 (s, 1H), 6.71-6.79 (m, 1H), 4.30-4.38 (m, 2H), 4.15-4.23 (m, 2H), 3.56-3.69 (m, 6H), 3.33 (s, 3H), 2.74-2.82 (m, 2H), 2.48-2.58 (m, 4H), 2.23 (s, 3H). 323 (DMSO-d6): 11.18 (br. s, 1H), 8.18 (d, J = 3.4 Hz, 1H), 7.66-7.75 (m, 2H), 7.32 562.2 (d, J = 8.6 Hz, 1H), 7.00 (s, 1H), 6.91 (d, J = 8.6 Hz, 1H), 6.70-6.78 (m, 2H), 4.27-4.35 (m, 2H), 4.16 (t, J = 5.7 Hz, 2H), 3.60-3.66 (m, 2H), 3.33 (s, 3H), 2.76 (t, J = 5.6 Hz, 2H), 2.48-2.58 (s, 4H), 2.31-2.42 (m, 4H), 2.20 (s, 3H), 2.17 (s, 3H), 1.90 (s, 3H).

TABLE 2-5 324 (MeOD): 8.16-8.23 (m, 1H), 7.97 (d, J = 7.7 Hz, 1H), 7.30-7.60 (m, 4H), 6.90- 477.0 6.71 (m, 2H), 6.60 (s, 1H), 4.42-4.53 (m, 2H), 3.65-3.80 (m, 4H), 3.41 (s, 3H), 2.41 (s, 6H), 2.35 (s, 3H). 325 (DMSO-d6): 12.39 (s, 1H), 8.59 (d, J = 8.4 Hz, 1H), 8.44 (d, J = 7.8 Hz, 1H), 8.34 412.2 (d, J = 4.6 Hz, 1H), 7.98 (d, J = 2.4 Hz, 1H), 7.22 (dd, J = 7.9, 4.7 Hz, 1H), 6.89 (s, 1H), 4.21 (t, J = 6.3 Hz, 2H), 3.94-4.06 (m, 1H), 3.56 (t, J = 5.9 Hz, 3H), 1.93-2.03 (m, 2H), 1.74-1.84 (m, 4H), 1.53-1.70 (m, 3H), 1.34-1.48 (m, 2H), 1.15-1.28 (m, 1H). 326 (DMSO-d6): 12.35 (s, 1H), 8.50 (br. s, 1H), 8.41 (d, J = 7.0 Hz, 1H), 8.30-8.35 425.2 (m, 1H), 7.98 (s, 1H), 7.20 (dd, J = 7.9, 4.7 Hz, 1H), 6.89 (s, 1H), 4.22 (t, J = 5.8 Hz, 2H), 3.94-4.06 (m, 1H), 2.51-2.58 (m, 2H), 2.23 (s, 6H), 1.96-2.05 (m, 2H), 1.75-1.86 (m, 2H), 1.62-1.72 (m, 1H), 1.34-1.59 (m, 4H), 1.16-1.29 (m, 1H). 327 (DMSO-d6): 12.30 (s, 1H), 8.37 (d, J = 7.8 Hz, 1H), 8.31 (d, J = 4.2 Hz, 1H), 7.96 425.2 (s, 1H), 7.19 (dd, J = 7.9, 4.4 Hz, 1H), 6.85 (s, 1H), 4.21 (t, J = 5.7 Hz, 2H), 2.40-2.60 (m, 6H), 2.22 (s, 6H), 1.73-1.87 (m, 2H), 1.15-1.42 (m, 3H), 0.97 (d, J = 6.1 Hz, 3H). 328 (DMSO-d6): 8.37 (d, J = 7.8 Hz, 1H), 8.31 (d, J = 3.6 Hz, 1H), 7.93 (s, 1H), 7.20- 412.2 7.27 (m, 1H), 6.85 (s, 1H), 4.17 (t, J = 6.2 Hz, 2H), 3.70-3.90 (m, 4H), 3.50- 3.56 (m, 2H), 1.70-1.88 (m, 6H), 1.50-1.60 (m, 4H). 329 (DMSO-d6): 12.42 (s, 1H), 8.43 (d, J = 7.9 Hz, 1H), 8.34 (d, J = 4.2 Hz, 1H), 7.98 412.2 (s, 1H), 7.24 (dd, J = 7.9, 4.8 Hz, 1H), 6.88 (s, 1H), 4.35-4.13 (m, 5H), 3.56 (t, J = 6.0 Hz, 2H), 3.25-3.37 (m, 2H), 1.70-1.88 (m, 5H), 1.26-1.40 (m, 2H), 0.96 (d, J = 6.2 Hz, 3H). 330 (DMSO-d6): 8.12-8.17 (m, 1H), 7.72-7.79 (m, 1H), 7.65 (s, 1H), 7.14-7.22 (m, 533.6 1H), 6.94 (s, 1H), 6.82-6.88 (m, 1H), 6.73-6.80 (m, 1H), 6.55 (s, 1H), 4.21- 4.30 (m, 2H), 4.09-4.16 (m, 2H), 3.55-3.66 (m, 2H), 3.32 (s, 3H), 2.85 (t, J = 5.7 Hz, 2H), 2.54-2.61 (m, 4H), 2.16 (s, 3H), 1.90 (s, 3H), 1.68-1.76 (m, 4H). 331 (DMSO-d6): 12.47 (s, 1H), 8.47 (d, J = 7.9 Hz, 1H), 8.36 (br. s, 1H), 8.09 (s, 446.0 1H), 7.16-7.32 (m, 6H), 6.93 (s, 1H), 5.00 (br. s, 2H), 4.22 (t, J = 6.4 Hz, 2H), 3.80-4.10 (m, 2H), 3.57 (t, J = 6.0 Hz, 2H), 3.08 (t, J = 5.6 Hz, 2H), 1.75-1.85 (m, 2H). 332 (DMSO-d6): 12.39 (s, 1H), 10.14 (s, 1H), 8.95 (d, J = 8.0 Hz, 1H), 8.34 (d, J = 415.1 4.5 Hz, 1H), 8.12 (d, J = 2.6 Hz, 1H), 7.24 (dd, J = 8.0, 4.7 Hz, 1H), 6.88 (s, 1H), 4.21 (t, J = 6.3 Hz, 2H), 3.76-3.86 (m, 4H), 3.53-3.61 (m, 3H), 3.02-3.12 (m, 4H), 1.87-1.74 (m, 2H). 333 (DMSO-d6): 12.38 (s, 1H), 9.95 (br. s, 1H), 8.87 (d, J = 7.7 Hz, 1H), 8.32 (d, J = 373.2 3.4 Hz, 1H), 8.03-8.09 (m, 1H), 7.23 (dd, J = 8.0, 4.7 Hz, 1H), 6.88 (s, 1H), 4.21 (t, J = 6.3 Hz, 2H), 3.57 (t, J = 5.8 Hz, 2H), 3.39 (s, 1H), 2.77 (s, 6H), 1.75- 1.85 (m, 2H). 334 (DMSO-d6): 12.31 (br. s, 1H), 8.91 (d, J = 7.53 Hz, 1H), 8.33 (d, J = 3.51 Hz, 415.2 1H), 8.09 (br. s, 1H), 7.22 (dd, J = 4.64, 7.91 Hz, 1H), 6.83 (br. s, 1H), 4.27 (t, J = 4.64 Hz, 2H), 3.77-3.85 (m, 4H), 3.56-3.62 (m, 2H), 3.30 (s, 3H), 3.00- 3.10 (m, 4H). 335 (DMSO-d6): 12.37 (br. s, 1H), 8.40 (dd, J = 1.26, 8.03 Hz, 1H), 8.33 (dd, J = 1.38, 406.2 4.64 Hz, 1H), 8.06 (s, 1H), 7.63 (d, J = 1.51 Hz, 1H), 7.21 (dd, J = 4.64, 7.91 Hz, 1H), 6.92 (s, 1H), 6.47 (d, J = 1.76 Hz, 1H), 4.90 (br. s, 2H), 4.21 (q, J = 7.19 Hz, 2H), 3.95-4.05 (m, 2H), 2.74-2.83 (m, 2H), 1.26 (t, J = 7.03 Hz, 3H). 336 (DMSO-d6): 12.27 (s, 1H), 10.19 (s, 1H), 8.18 (d, J = 3.5 Hz, 1H), 7.80-7.86 (m, 479.2 1H), 7.68 (d, J = 7.8 Hz, 1H), 7.16 (d, J = 8.4 Hz, 1H), 6.76-6.84 (m, 2H), 6.62 (s, 1H), 6.58 (d, J = 8.5 Hz, 1H), 4.26 (t, J = 6.3 Hz, 2H), 3.54-3.66 (m, 4H), 3.20 (t, J = 6.2 Hz, 3H), 2.13 (s, 3H), 1.78-1.87 (m, 2H). 337 (DMSO-d6): 12.36 (s, 1H), 10.61 (br. s, 1H), 10.54 (s, 1H), 8.24 (d, J = 3.8 Hz, 477.4 1H), 7.97 (d, J = 8.6 Hz, 1H), 7.55-7.70 (m, 4H), 6.89-6.95 (m, 2H), 4.38 (d, J = 4.8 Hz, 2H), 4.29 (t, J = 6.3 Hz, 2H), 3.62-3.55 (m, 2H), 3.39 (s, 1H), 2.76 (d, J = 4.6 Hz, 6H), 2.32 (s, 3H), 1.80-1.89 (m, 2H). 338 (DMSO-d6): 12.23 (s, 1H), 8.78 (d, J = 7.5 Hz, 1H), 8.29 (d, J = 4.1 Hz, 1H), 8.01 386.4 (s, 1H), 7.19 (dd, J = 7.9, 4.7 Hz, 1H), 6.77 (s, 1H), 4.20 (t, J = 5.8 Hz, 2H), 2.73 (s, 6H), 2.53-2.58 (m, 2H), 2.24 (s, 6H), 1.90 (s, 3H). 339 (DMSO-d6): 8.71 (d, J = 7.1 Hz, 1H), 8.27 (d, J = 4.6 Hz, 1H), 7.89 (s, 1H), 7.15 428.4 (dd, J = 7.8, 4.6 Hz, 1H), 6.37 (s, 1H), 4.10 (t, J = 5.9 Hz, 3H), 3.76-3.82 (m, 4H), 2.81-2.88 (m, 4H), 2.45-2.55 (m, 2H), 2.24 (s, 6H), 1.88 (s, 3H). 340 (DMSO-d6): 12.23 (br. s, 1H), 8.17 (d, J = 3.7 Hz, 1H), 7.80 (s, 1H), 7.67-7.74 492.4 (m, 1H), 7.08-7.17 (m, 1H), 6.78-6.84 (m, 1H), 6.70-6.78 (m, 1H), 6.60 (s, 1H), 6.53-6.58 (m, 1H), 5.81 (br. s, 1H), 4.71-4.79 (m, 1H), 4.21-4.30 (m, 2H), 3.59-3.67 (m, 2H), 3.15-3.25 (m, 2H), 2.55-2.60 (m, 2H), 2.21 (s, 6H), 2.12 (s, 3H), 1.90 (s, 3H). 341 (DMSO-d6): 12.28 (br. s, 1H), 10.45 (s, 1H), 8.18 (dd, J = 1.38, 4.64 Hz, 1H), 7.70- 429.1 7.76 (m, 2H), 7.55 (d, J = 8.03 Hz, 1H), 7.43 (s, 1H), 7.36 (d, J = 7.78 Hz, 1H), 6.85 (s, 1H), 6.77 (dd, J = 4.64, 7.91 Hz, 1H), 4.27 (q, J = 7.03 Hz, 2H), 4.17 (s, 2H), 2.28 (s, 3H), 1.30 (t, J = 7.15 Hz, 3H) 342 (DMSO-d6): 12.19 (br. s., 1H), 9.17 (s, 1H), 8.12-8.24 (m, 3H), 7.82 (d, J = 8.03 490.2 Hz, 1H), 7.66 (s, 1H), 7.21-7.40 (m, 3H), 6.78 (dd, J = 4.64, 7.91 Hz, 1H), 6.72 (s, 1H), 4.29 (t, J = 5.77 Hz, 2H), 3.52 (s, 2H), 2.65-2.73 (m, 2H), 2.30 (s, 6H), 2.23 (s, 9H). 343 (DMSO-d6): 12.39 (br. s, 1H), 9.93 (br. s, 1H), 8.28 (dd, J = 1.26, 4.52 Hz, 1H), 463.9 7.86 (s, 1H), 7.33 (d, J = 8.53 Hz, 2H), 7.10-7.19 (m, 2H), 7.00 (dd, J = 2.76, 8.53 Hz, 1H), 5.49 (br. s, 1H), 5.04-5.17 (m, 1H), 4.99 (t, J = 5.40 Hz, 1H), 4.12 (t, J = 4.89 Hz, 2H), 3.81 (q, J = 4.94 Hz, 2H), 2.23 (s, 3H), 1.28 (d, J = 6.27 Hz, 6H)

TABLE 2-6 344 (DMSO-d6): 12.33 (s, 1H), 10.07 (s, 1H), 8.93 (d, J = 7.8 Hz, 1H), 8.33 (d, J = 399.0 3.6 Hz, 1H), 8.11 (s, 1H), 7.22 (dd, J = 8.0, 4.6 Hz, 1H), 6.86 (s, 1H), 4.11 (t, J = 6.7 Hz, 2H), 3.76-3.86 (s, 4H), 3.02-3.13 (s, 4H), 1.60-1.72 (m, 2H), 0.94 (t, J = 7.4 Hz, 3H). 345 (DMSO-d6): 12.34 (s, 1H), 10.07 (s, 1H), 8.92 (d, J = 7.5 Hz, 1H), 8.33 (d, J = 413.4 3.3 Hz, 1H), 8.11 (d, J = 2.5 Hz, 1H), 7.22 (dd, J = 8.0, 4.7 Hz, 1H), 6.86 (s, 1H), 3.94 (d, J = 6.6 Hz, 2H), 3.75-3.87 (m, 4H), 3.00-3.15 (m, 4H), 1.88-2.02 (m, 1H), 0.95 (d, J = 6.7 Hz, 6H). 346 (DMSO-d6): 12.02 (br. s, 1H), 8.89 (d, J = 7.7 Hz, 1H), 8.32 (d, J = 3.4 Hz, 1H), 401.4 8.07 (s, 1H), 7.21 (dd, J = 8.0, 4.7 Hz, 1H), 6.77 (s, 1H), 4.15 (t, J = 5.4 Hz, 2H), 3.73-3.87 (m, 4H), 3.63 (t, J = 5.4 Hz, 2H), 3.17 (s, 1H), 2.95-3.07 (m, 4H). 347 (DMSO-d6): 12.35 (s, 1H), 10.09 (s, 1H), 8.94 (d, J = 7.9 Hz, 1H), 8.33 (d, J = 411.3 3.5 Hz, 1H), 8.12 (d, J = 2.4 Hz, 1H), 7.22 (dd, J = 8.0, 4.7 Hz, 1H), 6.87 (s, 1H), 4.02 (d, J = 7.1 Hz, 2H), 3.72-3.90 (m, 4H), 3.00-3.16 (m, 4H), 1.12-1.23 (m, 1H), 0.48-0.58 (m, 2H), 0.26-0.37 (m, 2H). 348 (DMSO-d6): 12.34 (s, 1H), 10.08 (s, 1H), 8.93 (d, J = 7.7 Hz, 1H), 8.33 (d, J = 425.4 4.5 Hz, 1H), 8.12 (d, J = 2.6 Hz, 1H), 7.22 (dd, J = 8.0, 4.7 Hz, 1H), 6.82 (s, 1H), 5.15-5.22 (m, 1H), 3.75-3.87 (m, 4H), 3.02-3.13 (m, 4H), 1.83-1.96 (m, 2H), 1.63-1.78 (m, 4H), 1.50-1.62 (m, 2H). 349 (DMSO-d6): 12.24 (br. s, 1H), 10.20 (br. s, 1H), 8.88 (d, J = 7.9 Hz, 1H), 8.32 413.6 (d, J = 3.5 Hz, 1H), 8.07 (s, 1H), 7.21 (dd, J = 8.0, 4.7 Hz, 1H), 6.72 (s, 1H), 4.94-4.79 (m, 1H), 3.74-3.88 (m, 4H), 2.90-3.14 (m, 4H), 1.48-1.70 (m, 2H), 1.23 (d, J = 6.2 Hz, 3H), 0.91 (t, J = 7.4 Hz, 3H). 350 (DMSO-d6): 12.48-12.19 (m, 1H), 10.04 (br. s, 1H), 8.93 (s, 1H), 8.33 (d, J = 413.4 3.7 Hz, 1H), 8.10 (s, 1H), 7.22 (dd, J = 7.9, 4.7 Hz, 1H), 6.81 (s, 1H), 4.80-4.96 (m, 1H), 3.74-3.87 (m, 4H), 3.01-3.14 (m, 4H), 1.50-1.68 (m, 2H), 1.24 (d, J = 6.2 Hz, 3H), 0.91 (t, J = 7.3 Hz, 3H). 351 (DMSO-d6): 12.34 (br. s, 1H), 9.86 (br. s, 1H), 8.97 (d, J = 7.8 Hz, 1H), 8.33 (d, 480.2 J = 3.4 Hz, 1H), 8.11 (s, 1H), 7.25 (dd, J = 8.0, 4.7 Hz, 1H), 6.80 (s, 1H), 5.00- 5.09 (m, 1H), 3.30-3.40 (m, 2H), 3.01-3.13 (m, 4H), 2.81-2.94 (m, 4H), 1.27 (d, J = 6.3 Hz, 6H). 352 (DMSO-d6): 12.35 (br. s, 1H), 8.62 (br. s, 1H), 8.40 (dd, J = 8.0, 1.3 Hz, 1H), 398.0 8.32 (d, J = 4.6 Hz, 1H), 8.01 (s, 1H), 7.21 (dd, J = 7.9, 4.7 Hz, 1H), 6.86 (s, 1H), 4.99-5.13 (m, 1H), 4.18-4.31 (m, 1H), 3.87-3.99 (m, 2H), 3.39-3.54 (m, 2H), 1.80-1.97 (m, 4H), 1.27 (d, J = 6.3 Hz, 6H). 353 (DMSO-d6): 12.36 (s, 1H), 10.43 (br. s, 1H), 8.88 (d, J = 6.5 Hz, 1H), 8.35 (d, 447.2 J = 3.5 Hz, 1H), 8.14 (s, 1H), 7.17 (dd, J = 8.0, 4.7 Hz, 1H), 6.86 (s, 1H), 4.99- 5.11 (m, 1H), 3.47-3.62 (m, 4H), 3.35-3.42 (m, 4H), 1.27 (d, J = 6.2 Hz, 6H). 354 (DMSO-d6): 12.36 (br. s, 1H), 8.39 (d, J = 7.9 Hz, 1H), 8.33 (d, J = 4.7 Hz, 1H), 460.2 8.07 (s, 1H), 7.16-7.26 (m, 2H), 6.80-6.92 (m, 3H), 5.01-5.12 (m, 1H), 4.94 (br. s, 2H), 3.83-4.02 (m, 2H), 3.76 (s, 3H), 3.00 (t, J = 5.7 Hz, 2H), 1.27 (d, J = 6.2 Hz, 6H). 355 (DMSO-d6): 13.04 (br. s, 1H), 8.40 (dd, J = 7.9, 1.4 Hz, 1H), 8.33 (dd, J = 4.6, 444.2 1.3 Hz, 1H), 8.07 (s, 1H), 7.22 (dd, J = 7.9, 4.7 Hz, 1H), 7.16 (d, J = 7.7 Hz, 1H), 7.08 (d, J = 7.1 Hz, 2H), 6.87 (s, 1H), 5.00-5.12 (m, 1H), 4.93 (br. s, 2H), 3.88-3.98 (m, 2H), 3.03 (t, J = 5.5 Hz, 2H), 2.30 (s, 3H), 1.89 (s, 3H), 1.28 (d, J = 6.2 Hz, 6H). 356 (DMSO-d6): 11.33 (br. s, 1H), 8.39 (dd, J = 7.9, 1.4 Hz, 1H), 8.33 (dd, J = 4.6, 464.2 1.3 Hz, 1H), 8.09 (s, 1H), 7.42 (s, 1H), 7.32 (s, 2H), 7.22 (dd, J = 7.9, 4.7 Hz, 1H), 6.88 (s, 1H), 4.94-5.11 (m, 3H), 3.87-4.00 (m, 2H), 3.07 (t, J = 5.7 Hz, 2H), 1.89 (s, 3H), 1.27 (t, J = 6.0 Hz, 6H). 357 (MeOD): 9.17 (s, 1H), 8.16-8.34 (m, 3H), 7.19-7.27 (m, 3H), 6.94-7.04 (m, 448.0 2H), 5.14-5.28 (m, 1H), 4.93 (br. s, 2H), 3.99 (t, J = 5.8 Hz, 2H), 3.05 (t, J = 5.5 Hz, 2H), 1.37 (d, J = 6.3 Hz, 6H). 358 (DMSO-d6): 12.36 (s, 1H), 8.39 (d, J = 7.9 Hz, 1H), 8.33 (d, J = 4.6 Hz, 1H), 8.06 460.2 (s, 1H), 7.18-7.28 (m, 2H), 6.81-6.94 (m, 3H), 5.00-5.11 (m, 1H), 4.96 (s, 2H), 3.91-4.06 (m, 2H), 3.81 (s, 3H), 2.94 (t, J = 5.7 Hz, 2H), 1.28 (d, J = 6.2 Hz, 6H). 359 (DMSO-d6): 12.34 (br. s, 1H), 8.39 (dd, J = 7.9, 1.1 Hz, 1H), 8.33 (dd, J = 4.6, 460.4 1.1 Hz, 1H), 8.06 (s, 1H), 7.17-7.25 (m, 2H), 6.82-6.90 (m, 3H), 5.00-5.10 (m, 1H), 4.89 (br. s, 2H), 3.84-4.00 (m, 2H), 3.76 (s, 3H), 3.05 (t, J = 5.7 Hz, 2H), 1.27 (d, J = 6.2 Hz, 6H).

Biological activities of several compounds of the present invention were measured according to the methods mentioned blow.

[Preparation and Storage of Solutions of Test Compounds]

A test compound was dissolved or suspended in DMSO so as to be at 10 mM or 1 mM, thereby making a stock solution, which was stored at −20° C. in the dark before being tested.

Test Example 1

[Inhibitory Effect on Cdc7 Protein Kinase]

Measurement of kinase activity was carried out using an MSA assay kit (QuickScout Screening Assist™ Kit, Carna Biosciences, Inc.).

Assay buffer (20 mM HEPES, 0.01% Triton X-100™, 2 mM dithiothreitol, pH 7.5) was used to prepare a substrate mixture solution comprising 4 μM of a kinase-reaction substrate (FITC-labeled MCM2 peptide), 40 mM MgCl₂, and 20 μM ATP.

The enzyme supplied in the kit (human Cdc7/human ASK complex protein) was diluted in the assay buffer so as to be at 7 nM, thereby preparing an enzyme solution.

The stock solution of each test compound was diluted in DMSO to prepare diluted DMSO solutions of 10 concentrations (0.00003 mM, 0.0001 mM, 0.0003 mM, 0.001 mM, 0.003 mM, 0.01 mM, 0.03 mM, 0.1 mM, 0.3 mM, 1 mM), each of which was further diluted 25 times in the assay buffer to make a drug solution (a solution containing 4% DMSO).

The kinase reaction was performed in wells of a polypropylene 384-well plate in which 5 μl of each of the drug solutions or of a solvent (assay buffer containing 4% DMSO), 5 μl of the substrate mixture solution, and 10 μl of the enzyme solution were mixed. The assay buffer, instead of the enzyme solution, was added to blank wells. After the reaction at room temperature for 5 hours, 60 μl of the termination buffer supplied in the kit was added to each well to stop the reaction. The percent inhibition of each reaction was determined using a LabChip EZ Reader II system (manufactured by Caliper Life Sciences), according to the protocol provided with the assay kit. In this case, the peak heights of the separated substrate and the phosphorylated substrate were represented as S and P, respectively, and the percent inhibition (%) by a tested compound was calculated according to the following formula: Percent inhibition(%)=(1−(C−A)/(B−A))×100 wherein A denotes P/(P+S) for a blank well, B denotes P/(P+S) for a solvent well, and C denotes P/(P+S) for a compound-added well.

The IC₅₀ value of a tested compound was calculated by regression analysis of the percent inhibition values versus the (logarithmic) concentrations of the tested compound.

The IC₅₀ values of several compounds of the present invention against Cdc7 were all less than 1 μM, as shown in Table 4 below.

Test Example 2

[Inhibitory Effect on Phosphorylation Using Cells]

Cultured Cells

COLO205 cells derived from human colon adenocarcinoma (RCB2127, RIKEN BRC) were cultured in 10 cm dishes using RPMI-1640 medium (SIGMA, R8758) supplemented with 10% FCS (Equitech-bio) and 5% penicillin/streptomycin (GIBCO, 15140). After the cells were cultured to a confluence of 70 to 90%, the medium was removed, and the cells were treated with trypsin (2 mL, TrypLE Express, GIBCO, 12604-021) and then harvested using a new medium.

Addition of Drugs to Cultured Cells

COLO205 cells were seeded in 6-well plates (FALCON, 35046) to 2.5×10⁵ cells (1 ml) per well and cultured overnight in a 5% CO₂ incubator. A drug solution, which was prepared by adding 1.5 μl of a 0.1-mM stock solution in DMSO of a test compound to 500 μl of medium, was added to each well (to a final drug concentration of 0.1 μM), and then cultured for another 24 hours.

Determination of Inhibitory Effect on MCM2 Phosphorylation

Cells were cultured for 24 hours in the presence of a test compound, harvested using a similar way as described above, washed with PBS, and then pelletized and stored at −80° C. The frozen cells were thawed, subsequently suspended immediately in 2×SDS-PAGE electrophoresis buffer (1×10⁵ cells per 10 μl), and heated at 95° C. for 10 minutes, leading to the dissolution and denaturation of the proteins. The resulting sample solutions were subjected to SDS-PAGE using a 5-20% gradient acrylamide gel (e-PAGEL (5-20%), ATTO, E-T520L). After the electrophoresis was completed, the gel was immersed in tris-glycine buffer supplemented with 20% methanol, and the proteins in the gel were transferred onto a PVDF membrane (MILLIPORE, Immobilon-P, IPVH00010) using a semi-dry transfer apparatus (TRNS-BLOT SD SEMI-DRY TRANSFER CELL, BIO RAD).

The transferred PVDF membrane was blocked with 5% skim milk (Difco Skim Milk, BD, 232100), and then subjected to reaction with an anti-MCM2 goat antibody (N-19, Santa Cruz, sc-9839) or anti-phosphorylated MCM2 rabbit antibody (S53) (Bethyl, A300-756A) as a primary antibody and additionally with an anti-α tubulin mouse antibody (Clone DM 1A, SIGMA, 9026) as an internal control. Each band was detected by chemiluminescence using an HRP-labeled anti-goat IgG donkey antibody (Santa Cruz, sc2020), anti-rabbit IgG sheep antibody (Roche, 12015218001), or anti-mouse IgG donkey antibody (Jackson ImmunoResearch, 715-035-151) as a secondary antibody. The combinations of and the diluted concentrations of the primary and secondary antibodies were as indicated below.

TABLE 3 Primary antibody Secondary antibody (diluted concentration) (diluted concentration) 1 Anti-MCM2 goat antibody Anti-goat IgG donkey (1/500) antibody (1/10000) 2 Anti-phosphorylated MCM2 Anti-rabbit IgG sheep rabbit antibody (S53) antibody (1/5000) (1/500) 3 Anti-α-tubulin mouse Anti-mouse IgG donkey antibody (1/1000) antibody (1/100000)

The bands which were detected were normalized using the amount of endogenous α-tubulin as a reference, and the percent phosphorylation of MCM2 was calculated for each of the tested compounds. Their inhibitory effects on MCM2 phosphorylation were indicated by a triple asterisk (***) in the case where the percent phosphorylation of MCM2 was less than 20%, by a dual asterisk (**) in the case where the percent phosphorylation of MCM2 was not less than 20% and less than 50%, and by a single asterisk (*) in the case where the percent phosphorylation of MCM2 was not less than 50% and less than 70%.

It was found from this testing that as shown in Table-4, the tested compounds of the present invention inhibited the phosphorylation of MCM2 at a concentration of 0.1 μM.

TABLE 4 Test Example 2 Test Example 1 Inhibitory effect on Example Cdc7 IC₅₀ value (μM) MCM2 phosphorylation 15 0.002 ** 22 0.010 * 63 0.006 *** 68 0.004 *** 78 0.011 *** 81 0.005 *** 89 0.003 *** 91 0.004 *** 102 0.004 *** 126 0.003 *** 129 0.009 *** 142 0.003 ** 157 0.003 ** 173 0.003 *** 226 0.004 *** 233 0.002 ** 239 0.001 ** 242 0.001 *** 244 0.004 *** 245 0.008 *** 246 0.008 ***

INDUSTRIAL APPLICABILITY

The compounds provided by the present invention are capable of controlling the growth of cells through their inhibitory effects on Cdc7. Therefore, the compounds of the present invention which have an inhibitory effect on Cdc7 will be useful as a medicine, especially an agent for the treatment of diseases derived from abnormal growth of cells, such as cancers. 

The invention claimed is:
 1. A furanone derivative or a pharmaceutically acceptable salt thereof, represented by the formula (I): [Chemical Formula 1]

the formula (I-Z) or the formula (I-E):

wherein A represents —COOR1 or a hydrogen atom; R1 represents a hydrogen atom, an optionally substituted hydrocarbon group, or an optionally substituted heterocycle; R2 and R3 are the same or different and each independently represent a hydrogen atom, an optionally substituted hydrocarbon group, an optionally substituted phenyl group, an optionally substituted heterocycle, an optionally substituted heterocyclic fused ring, or an optionally substituted amino group; or alternatively, R2 and R3, taken together with the nitrogen atom to which they are attached, form an optionally substituted heterocycle or an optionally substituted heterocyclic fused ring; and R4 represents a hydrogen atom or a halogen atom; with the proviso that when A represents —COOR1, R2 and R3 are not optionally substituted amino groups at the same time, and when A represents a hydrogen atom, R3 represents a hydrogen atom.
 2. The furanone derivative or a pharmaceutically acceptable salt thereof according to claim 1, wherein A is —COOR1.
 3. The furanone derivative or a pharmaceutically acceptable salt thereof according to claim 1, wherein A is a hydrogen atom.
 4. The furanone derivative or a pharmaceutically acceptable salt thereof according to claim 1, wherein the furanone derivative or a pharmaceutically acceptable salt thereof is represented by the formula (II-Z) or formula (II-E):

wherein A represents —COOR1 or a hydrogen atom; R1 is selected from the group consisting of a hydrogen atom, an optionally substituted 1 to 6 carbon hydrocarbon group, and an optionally substituted 3 to 8 membered heterocycle; R2 and R3, taken together with the nitrogen atom to which they are attached, form an optionally substituted 3 to 8 membered heterocycle or an optionally substituted 3 to 8 membered heterocyclic fused ring; R4 represents a hydrogen atom or a halogen atom; wherein any and all heterocyclic groups contain up to four heteroatoms selected from the group consisting of nitrogen, sulfur, and oxygen; with the proviso that when A represents —COOR1, R2 and R3 are not optionally substituted amino groups at the same time, and when A represents a hydrogen atom, R3 represents a hydrogen atom.
 5. The furanone derivative or a pharmaceutically acceptable salt thereof according to claim 1, wherein any and all heterocyclic groups are optionally substituted 3 to 8 membered heterocycle groups that contain up to two heteroatoms selected from the group consisting of nitrogen, sulfur, and oxygen.
 6. The furanone derivative or a pharmaceutically acceptable salt thereof according to claim 1, wherein any and all hydrocarbon groups are 1 to 6 carbon hydrocarbon groups; and wherein any and all heterocyclic groups are optionally substituted 3 to 8 membered heterocycle groups that contain up to two heteroatoms selected from the group consisting of nitrogen, sulfur, and oxygen.
 7. The furanone derivative or a pharmaceutically acceptable salt thereof according to claim 4, wherein A and R4 are both a hydrogen atom and R2 and R3 form a 3 to 8 membered heterocycle.
 8. The furanone derivative or a pharmaceutically acceptable salt thereof according to claim 4, wherein A is —COOR1 where R1 is an optionally substituted 1 to 6 carbon hydrocarbon group, R4 is a hydrogen atom and R2 and R3 form a 3 to 8 membered heterocycle containing up to two heteroatoms selected from the group consisting of nitrogen, sulfur, and oxygen.
 9. The furanone derivative or a pharmaceutically acceptable salt thereof according to claim 1, wherein A is —COOR1 where R1 is an optionally substituted 1 to 6 carbon hydrocarbon group, R4 is a hydrogen atom and one of either of R2 and R3 is a hydrogen atom.
 10. The furanone derivative or a pharmaceutically acceptable salt thereof according to claim 1, wherein A is —COOR1 where R1 is an optionally substituted 1 to 6 carbon hydrocarbon group, R4 is a fluorine atom and one of either of R2 and R3 is a hydrogen atom.
 11. The furanone derivative or a pharmaceutically acceptable salt thereof according to claim 1, wherein A and R4 are both a hydrogen atom, and one of either of R2 and R3 is a hydrogen atom.
 12. The furanone derivative or a pharmaceutically acceptable salt thereof according to claim 1, wherein A is —COOR1 where R1 is an optionally substituted 1 to 6 carbon hydrocarbon group, R4 is a hydrogen atom, one of either of R2 and R3 is a hydrogen atom and one of either of R2 and R3 is an optionally substituted amino group.
 13. The furanone derivative or a pharmaceutically acceptable salt thereof according to claim 1, wherein A is —COOR1 where R1 is an optionally substituted 1 to 6 carbon hydrocarbon group, R4 is a hydrogen atom, one of either of R2 and R3 is an optionally substituted 1 to 6 carbon hydrocarbon group and one of either of R2 and R3 is a 3 to 8 membered heterocycle containing up to two heteroatoms selected from the group consisting of nitrogen, sulfur, and oxygen.
 14. The furanone derivative or a pharmaceutically acceptable salt thereof according to claim 1, wherein A is —COOR1 where R1 is an optionally substituted 1 to 6 carbon hydrocarbon group, R4 is a hydrogen atom, one of either of R2 and R3 is a hydrogen atom and one of either of R2 and R3 is an optionally substituted 3 to 8 membered heterocyclic fused ring.
 15. The furanone derivative or a pharmaceutically acceptable salt thereof according to claim 1, wherein A is —COOR1 where R1 is a 3 to 8 membered heterocycle containing up to two heteroatoms selected from the group consisting of nitrogen, sulfur, and oxygen, R4 is a hydrogen atom and one of either of R2 and R3 is a hydrogen atom.
 16. The furanone derivative or a pharmaceutically acceptable salt thereof according to claim 1, wherein the furanone derivative or pharmaceutically acceptable salt thereof is selected from the group consisting of:


17. The furanone derivative or a pharmaceutically acceptable salt thereof according to claim 1, wherein the furanone derivative or pharmaceutically acceptable salt thereof is selected from the group consisting of:


18. The furanone derivative or a pharmaceutically acceptable salt thereof according to claim 1, wherein the furanone derivative or pharmaceutically acceptable salt thereof is selected from the group consisting of:


19. The furanone derivative or a pharmaceutically acceptable salt thereof according to claim 1, wherein the pharmaceutically acceptable salt is the hydrochloride or p-toluenesulfonate salt.
 20. The furanone derivative or a pharmaceutically acceptable salt thereof according to claim 16, wherein the pharmaceutically acceptable salt is the hydrochloride or p-toluenesulfonate salt. 